Objective:To investigate the anti-obesity activity and the action mechanism of the roots of Adenophora triphylla var.japonica extract(ATE) in high-fat diet(HFD)-induced obese mice and 3T3-LI adipocytes.Methods:The roo...Objective:To investigate the anti-obesity activity and the action mechanism of the roots of Adenophora triphylla var.japonica extract(ATE) in high-fat diet(HFD)-induced obese mice and 3T3-LI adipocytes.Methods:The roots of Ademption triphylla were extracted with 70%ethanol.To demonstrate the compounds,linoleic acid was analyzed by using gas chromatography:and the anti-obesity effects and possible mechanisms of ATE were examined in 3T3-L1 adipocytes and HFD-induced obese mice.Results:Treatment with ATE inhibited the lipid accumulation without cytotoxicity in 3T3-L1 adipocytes.Furthermore,200 and 400 mg/kg ATE treatment significantly decreased the body weight gain,white adipose tissues(WATs) weight and plasma triglyceride level,while 100 and 200 mg/kg ATE treatment increased the plasma high-density lipoprotein cholesterol level in the HFD-induced obese mice,as compared with the HFD group.Treatment with 200 and 400 mg/kg ATE also lowered the size of adipocytes in adipose tissue and reduced the lipid accumulation in liver.ATE treatment showed significantly lower expression level of adipogenesis-relatcd proteins,such as peroxisome proliferator-activated receptor y,fatty acid binding protein(aP2),fatty acid synthase in 3T3-L1 adipocytes;and furthermore,decreased peroxisome proliferatoractivated receptor γ,aP2,fatty acid synthase,sterol regulatory element binding protein-1c,and lipoprotein lipase mRNA expression levels in WAT of the HFD-induced obese mice.Conclusions:These results suggested that the ATE has an anti-obesity effect,which may be elicited by regulating the expression of adipogenesis and lipogenesis-related genes and proteins in adipocytes and WAT of the HFD-induced obese mice.展开更多
Objective:To assess the anticancer activity of eight cyanobacterial hydrophilic extracts on Ehrlich ascites carcinoma cell line.Methods:The cyanobacterial strains used in the investigation were collected from diverse ...Objective:To assess the anticancer activity of eight cyanobacterial hydrophilic extracts on Ehrlich ascites carcinoma cell line.Methods:The cyanobacterial strains used in the investigation were collected from diverse habitats in Egypt.The initial cytotoxicity test of cyanobacterial hydrophilic extracts was carried out by MTT assay.The in vitro anticancer activity of the four most active extracts was performed on MCF-7 cells using sulforhodamine B assay.Morphological and molecular techniques were used to characterise identity of the isolate from which the most potent cytotoxic extract was obtained.Results:Extracts from four cyanobacterial strains had higher cytotoxic activities scoring76.68%,77.70%,76.70%and 74.45%,respectively.A considerable anticancer effect was only detected when the concentrated extracts were used.One cyanobacterial extract gave the highest anticancer activity on human breast adenocarcinoma cell line(57.6%of inhibition)as compared to control.The isolate was best-matched to Cyanothece sp.with sequence resemblance 98%to Cyanothece sp.strain PCC7564 and the phylogenetic analysis confirmed its close identity to the Cyanothece genus.Conclusions:This is the first study to report the anticancer effect of aqueous extracts derived from the unicellular Cyanothece sp.from Egypt and its potential as a plausible candidate for future mass biotechnological applications.展开更多
A novel MEMS inductor consisting of a planar single crystalline silicon spiral with a copper surface coating as the conductor is presented. Using a silicon-glass anodic bonding and deep etching formation-and-release p...A novel MEMS inductor consisting of a planar single crystalline silicon spiral with a copper surface coating as the conductor is presented. Using a silicon-glass anodic bonding and deep etching formation-and-release process,a 40μm-thick silicon spiral is formed, which is suspended on a glass substrate to eliminate substrate loss. The surfaces of the silicon spiral are coated with highly conformal copper by electroless plating to reduce the resis- tive loss in the conductor,with thin nickel film plated on the surface of the copper layer for final surface passivation. The fabricated inductor exhibits a self-resonance frequency higher than 15GHz,with a quality factor of about 40 and an inductance of over 5nil at 11.3GHz. Simulations based on a compact equivalent circuit model of the inductor and parameter extraction using a characteristic-function approach are carried out,and good agreement with measurements is obtained.展开更多
针对SAR图像数据集的特点,提出了一种基于像元级图像时间序列相似性的水体提取方法。其基本思想是:构建像元级SAR图像时间序列,选取动态时间归整(dynamic time warping,DTW)算法作为时间序列相似度的度量方法,计算所有像元与标准水体像...针对SAR图像数据集的特点,提出了一种基于像元级图像时间序列相似性的水体提取方法。其基本思想是:构建像元级SAR图像时间序列,选取动态时间归整(dynamic time warping,DTW)算法作为时间序列相似度的度量方法,计算所有像元与标准水体像元时间序列的相似性;将水体边缘混合像元的DTW距离值设定为参考阈值,采用阈值法提取相似性较高的时间序列数据,其对应的像元即被识别为水体像元;最后利用每个像元的DTW距离值代替其后向散射值,采用8邻域搜索方法提高水体识别的精度。以2008年1—12月获取的25景分辨率为150 m的ENVISAT ASAR图像进行水体像元提取试验,结果表明,该方法的完整率和正确率均较高,能够应用于大范围区域水体的提取与制图。展开更多
基金carried out with the support of Cooperative Reycarch Program for Agriculture Science&Technology Development(Project No.P1134801)Rural Development Administration.Republic of Kores
文摘Objective:To investigate the anti-obesity activity and the action mechanism of the roots of Adenophora triphylla var.japonica extract(ATE) in high-fat diet(HFD)-induced obese mice and 3T3-LI adipocytes.Methods:The roots of Ademption triphylla were extracted with 70%ethanol.To demonstrate the compounds,linoleic acid was analyzed by using gas chromatography:and the anti-obesity effects and possible mechanisms of ATE were examined in 3T3-L1 adipocytes and HFD-induced obese mice.Results:Treatment with ATE inhibited the lipid accumulation without cytotoxicity in 3T3-L1 adipocytes.Furthermore,200 and 400 mg/kg ATE treatment significantly decreased the body weight gain,white adipose tissues(WATs) weight and plasma triglyceride level,while 100 and 200 mg/kg ATE treatment increased the plasma high-density lipoprotein cholesterol level in the HFD-induced obese mice,as compared with the HFD group.Treatment with 200 and 400 mg/kg ATE also lowered the size of adipocytes in adipose tissue and reduced the lipid accumulation in liver.ATE treatment showed significantly lower expression level of adipogenesis-relatcd proteins,such as peroxisome proliferator-activated receptor y,fatty acid binding protein(aP2),fatty acid synthase in 3T3-L1 adipocytes;and furthermore,decreased peroxisome proliferatoractivated receptor γ,aP2,fatty acid synthase,sterol regulatory element binding protein-1c,and lipoprotein lipase mRNA expression levels in WAT of the HFD-induced obese mice.Conclusions:These results suggested that the ATE has an anti-obesity effect,which may be elicited by regulating the expression of adipogenesis and lipogenesis-related genes and proteins in adipocytes and WAT of the HFD-induced obese mice.
基金the University of Helwan,Egypt granting them analyses funds for anticancer bioassays and molecular work(grant application approved at Departmental meeting number 240,March 2014)
文摘Objective:To assess the anticancer activity of eight cyanobacterial hydrophilic extracts on Ehrlich ascites carcinoma cell line.Methods:The cyanobacterial strains used in the investigation were collected from diverse habitats in Egypt.The initial cytotoxicity test of cyanobacterial hydrophilic extracts was carried out by MTT assay.The in vitro anticancer activity of the four most active extracts was performed on MCF-7 cells using sulforhodamine B assay.Morphological and molecular techniques were used to characterise identity of the isolate from which the most potent cytotoxic extract was obtained.Results:Extracts from four cyanobacterial strains had higher cytotoxic activities scoring76.68%,77.70%,76.70%and 74.45%,respectively.A considerable anticancer effect was only detected when the concentrated extracts were used.One cyanobacterial extract gave the highest anticancer activity on human breast adenocarcinoma cell line(57.6%of inhibition)as compared to control.The isolate was best-matched to Cyanothece sp.with sequence resemblance 98%to Cyanothece sp.strain PCC7564 and the phylogenetic analysis confirmed its close identity to the Cyanothece genus.Conclusions:This is the first study to report the anticancer effect of aqueous extracts derived from the unicellular Cyanothece sp.from Egypt and its potential as a plausible candidate for future mass biotechnological applications.
文摘A novel MEMS inductor consisting of a planar single crystalline silicon spiral with a copper surface coating as the conductor is presented. Using a silicon-glass anodic bonding and deep etching formation-and-release process,a 40μm-thick silicon spiral is formed, which is suspended on a glass substrate to eliminate substrate loss. The surfaces of the silicon spiral are coated with highly conformal copper by electroless plating to reduce the resis- tive loss in the conductor,with thin nickel film plated on the surface of the copper layer for final surface passivation. The fabricated inductor exhibits a self-resonance frequency higher than 15GHz,with a quality factor of about 40 and an inductance of over 5nil at 11.3GHz. Simulations based on a compact equivalent circuit model of the inductor and parameter extraction using a characteristic-function approach are carried out,and good agreement with measurements is obtained.
文摘针对SAR图像数据集的特点,提出了一种基于像元级图像时间序列相似性的水体提取方法。其基本思想是:构建像元级SAR图像时间序列,选取动态时间归整(dynamic time warping,DTW)算法作为时间序列相似度的度量方法,计算所有像元与标准水体像元时间序列的相似性;将水体边缘混合像元的DTW距离值设定为参考阈值,采用阈值法提取相似性较高的时间序列数据,其对应的像元即被识别为水体像元;最后利用每个像元的DTW距离值代替其后向散射值,采用8邻域搜索方法提高水体识别的精度。以2008年1—12月获取的25景分辨率为150 m的ENVISAT ASAR图像进行水体像元提取试验,结果表明,该方法的完整率和正确率均较高,能够应用于大范围区域水体的提取与制图。
