The effects of Ce （Ⅳ） on callus growth, anthocyanin content, and expression of anthocyanin biosynthetic genes in callus suspension cultures of Solanum tuberosum cv. Chieftain were studied by the measurement of fres...The effects of Ce （Ⅳ） on callus growth, anthocyanin content, and expression of anthocyanin biosynthetic genes in callus suspension cultures of Solanum tuberosum cv. Chieftain were studied by the measurement of fresh weight, spectrophotometric assays, and semiquantitative RT-PCR. The results indicate that 0.1 mmol·L^- 1 Ce （ Ⅳ ） can promote callus growth, increase the accumulation of anthocyanins, and enhance the expression of five anthocyanin biosynthetic genes （ CHS, F3H, F3＇5＇H, DFR, and 3 GT） most efficiently. At high concentrations of 1 mmol·L^- 1, Ce （Ⅳ） partially inhibits callus growth and at 2 mmol· L^-1 eventually lends to cell death. The results show that Ce（ Ⅳ ） can induce the expression of anthocyanin biosynthetic genes to produce and accumulate anthocyanins and increase the yield of anthocyanins.展开更多
Cotton(Goss y pium hirsutum L.)is the leading fiber crop and one of the mainstays of the econo-my in the world.Cotton fibers,as the main product of cotton plants,are unicellular,linear structures derived from the epid...Cotton(Goss y pium hirsutum L.)is the leading fiber crop and one of the mainstays of the econo-my in the world.Cotton fibers,as the main product of cotton plants,are unicellular,linear structures derived from the epidermis of the ovule.Cotton fiber develop ment consists of fo ur discrete yet over-lapping developmentalstages:initiation,elongation,secondary wall deposition,and maturation.Therefore,it is regard as an ideal experimental model for studying plant cell elo ngation,cellulose syn-thesis,and cell wall deposition.Furt hermore,the fiber quantity and quality are established during fi-ber development.展开更多
The strategy of isolating the band-specific expression fragments from a probe pool generated by humanchromosome microdissection was reported. A cliromosome 14q24.3 band-specific single copy DNA pool was constructed ba...The strategy of isolating the band-specific expression fragments from a probe pool generated by humanchromosome microdissection was reported. A cliromosome 14q24.3 band-specific single copy DNA pool was constructed based on this probe pool. Using total DNA of the pool as probe to hybridize the human marrow cDNA library, 68 primary positive clones were selected from 5 ×105 cDNA clones. Among these primary clones, 32 secondary clones were obtained after second-round screening and designed as cFD14-1～32. Finally, 24 band-specific expression fragments were identified from these 32 positive clones by DNA hybridization. Those band-specific clones can hybridize to both 14q24.3 DNA and human genomic DNA but cann’t hybridize to 17q11～12 DNA. Partial sequences of 13 fragments of them were sequenced and identified as novel cDNA sequences , and these sequences were proved to have some homology with known genes in NCBI database. Analysis of expression spectrum of cFD14-1 suggested that the cDNA fragments thus obtained should be used to isolate the genes can not been cloned in 14q24.3region.展开更多
基金Project Supported bythe International Cooperation Research of Jiangsu Province (BZ2003041)
文摘The effects of Ce （Ⅳ） on callus growth, anthocyanin content, and expression of anthocyanin biosynthetic genes in callus suspension cultures of Solanum tuberosum cv. Chieftain were studied by the measurement of fresh weight, spectrophotometric assays, and semiquantitative RT-PCR. The results indicate that 0.1 mmol·L^- 1 Ce （ Ⅳ ） can promote callus growth, increase the accumulation of anthocyanins, and enhance the expression of five anthocyanin biosynthetic genes （ CHS, F3H, F3＇5＇H, DFR, and 3 GT） most efficiently. At high concentrations of 1 mmol·L^- 1, Ce （Ⅳ） partially inhibits callus growth and at 2 mmol· L^-1 eventually lends to cell death. The results show that Ce（ Ⅳ ） can induce the expression of anthocyanin biosynthetic genes to produce and accumulate anthocyanins and increase the yield of anthocyanins.
基金This work was supported by the National Natural Science Foundation of China(No 30370904and No 30671258)the National High Technology Research and Development Program(863 project)of China(No 2006AA10Z121)the Program for New Century Excellent Talents in University(No NCET-07-0712)
文摘Cotton(Goss y pium hirsutum L.)is the leading fiber crop and one of the mainstays of the econo-my in the world.Cotton fibers,as the main product of cotton plants,are unicellular,linear structures derived from the epidermis of the ovule.Cotton fiber develop ment consists of fo ur discrete yet over-lapping developmentalstages:initiation,elongation,secondary wall deposition,and maturation.Therefore,it is regard as an ideal experimental model for studying plant cell elo ngation,cellulose syn-thesis,and cell wall deposition.Furt hermore,the fiber quantity and quality are established during fi-ber development.
文摘The strategy of isolating the band-specific expression fragments from a probe pool generated by humanchromosome microdissection was reported. A cliromosome 14q24.3 band-specific single copy DNA pool was constructed based on this probe pool. Using total DNA of the pool as probe to hybridize the human marrow cDNA library, 68 primary positive clones were selected from 5 ×105 cDNA clones. Among these primary clones, 32 secondary clones were obtained after second-round screening and designed as cFD14-1～32. Finally, 24 band-specific expression fragments were identified from these 32 positive clones by DNA hybridization. Those band-specific clones can hybridize to both 14q24.3 DNA and human genomic DNA but cann’t hybridize to 17q11～12 DNA. Partial sequences of 13 fragments of them were sequenced and identified as novel cDNA sequences , and these sequences were proved to have some homology with known genes in NCBI database. Analysis of expression spectrum of cFD14-1 suggested that the cDNA fragments thus obtained should be used to isolate the genes can not been cloned in 14q24.3region.
