This study examined ionizing radiation-induced tumor necrosis factor （ligand） superfamily, member 4 （TNFSF4） mRNA expression changes in human peripheral blood cells and their distribution in a normal population. T...This study examined ionizing radiation-induced tumor necrosis factor （ligand） superfamily, member 4 （TNFSF4） mRNA expression changes in human peripheral blood cells and their distribution in a normal population. The results showed that expression level of TNFSF4 mRNA exhibited a dose- dependent response after different irradiation doses, but that was independent of incubation time post-irradiation. Moreover, it was not affected by age and gender in 51 healthy donors. Our studies indicate that TNFSF4 can be considered as a candidate gene to develop a new biodosimeter.展开更多
Wallerian degeneration （WD） remains an important research topic. Many genes are differentially expressed during the process of WD, but the precise mechanisms responsible for these differentiations are not completely...Wallerian degeneration （WD） remains an important research topic. Many genes are differentially expressed during the process of WD, but the precise mechanisms responsible for these differentiations are not completely understood. In this study, we used microarrays to analyze the expression changes of the distal nerve stump at 0, 1, 4, 7, 14, 21 and 28 days after sciatic nerve injury in rats. The data revealed 6 076 differentiatly-expressed genes, with 23 types of expression, specifically enriched in genes associated with nerve development and axonogenesis, cytokine biosynthesis, cell differentiation, cytokine/chemokine production, neuron differentiation, cytokinesis, phosphorylation and axon regeneration. Kyoto Encyclopedia of Genes and Genomes pathway analysis gave findings related mainly to the MAPK signaling pathway, the Jak-STAT signaling pathway, the cell cycle, cytokine-cytokine receptor interaction, the p53 signaling pathway and the Wnt signaling pathway. Some key factors were NGF, MAG, CNTF, CTNNA2, p53, JAK2, PLCB1, STAT3, BDNF, PRKC, collagen II, FGF, THBS4, TNC and c-Src, which were further validated by real-time quantitative PCR, Western blot, and immunohistochemistry. Our findings contribute to a better understanding of the functional analysis of differentially-expressed genes in WD and may shed light on the molecular mechanisms of nerve degeneration and regeneration.展开更多
Trichloroethylene （TCE） is a major pollutant that affects both occupational and general environments. The liver is an important target organ of TCEE. Substantial efforts and remarkable progress into understanding TC...Trichloroethylene （TCE） is a major pollutant that affects both occupational and general environments. The liver is an important target organ of TCEE. Substantial efforts and remarkable progress into understanding TCE cytotoxicity have been made in cultured liver cells. However, the molecular mechanisms by which TCE induces hepatotoxicity are not well understood. SET （also known as protein phosphatase 2A inhibitor, 12PP2A, or template-activating factor-I, TAF-D is a nuclear protein that regulates histone modification, gene transcription, DNA replication, nucleosome assembly,展开更多
Dear Editor,Rhesus macaques are valuable nonhuman animal models for studying the aging process,but the molecular dynamics of aging remain elusive.Although previous studies have examined gene expression changes in huma...Dear Editor,Rhesus macaques are valuable nonhuman animal models for studying the aging process,but the molecular dynamics of aging remain elusive.Although previous studies have examined gene expression changes in human and rodent aging models(Huan et al.,2018;Li et al.,2023;Schaum et al.,2020;Tumasian Iii et al.,2021),a longitudinal analysis of the aging transcriptome in non-human primates,which are evolutionarily close relatives to humans,is scarce.In this study,we conducted a time series analysis by sequencing the transcriptomes of peripheral blood from healthy female rhesus macaques,aged 7 to 29,to explore age-associated changes in gene expression(Detailed in supplementary methods).展开更多
Dear Editor,Post-traumatic stress disorder(PTSD)is a chronic neuropsychiatric disorder triggered by severe traumatic events,characterized by persistent intrusive memories,emotional dysregulation,hyperarousal,and avoid...Dear Editor,Post-traumatic stress disorder(PTSD)is a chronic neuropsychiatric disorder triggered by severe traumatic events,characterized by persistent intrusive memories,emotional dysregulation,hyperarousal,and avoidance behaviors[1,2].PTSD is associated with significant gene expression changes in key brain regions,including the ventral tegmental area(VTA),which may underlie dysregulation of dopaminergic signaling and stress-related behaviors[3].展开更多
AIM: To investigate the role of claudin 1 in the regulation of genes involved in cell migration and tumor necrosis factor alpha (TNF-α)-induced gene expression in human gastric adenocarcinoma cells.
基金supported by the National Key Clinical Special Department of China(Grant No.2011-17)the Medical Science and Technology Foundation of Henan Province(Grant No.201003146 and No.201303202)
文摘This study examined ionizing radiation-induced tumor necrosis factor （ligand） superfamily, member 4 （TNFSF4） mRNA expression changes in human peripheral blood cells and their distribution in a normal population. The results showed that expression level of TNFSF4 mRNA exhibited a dose- dependent response after different irradiation doses, but that was independent of incubation time post-irradiation. Moreover, it was not affected by age and gender in 51 healthy donors. Our studies indicate that TNFSF4 can be considered as a candidate gene to develop a new biodosimeter.
基金supported by grants from the National Natural Science Foundation of China (81130080, 30870811)the Scientific Research Foundation for Returned Overseas Chinese Scholars, Ministry of Education of China+1 种基金the Natural Science Foundation of Jiangsu Province, China (BK2010282)the Priority Academic Program Development of Higher Education Institutions of Jiangsu Province, China
文摘Wallerian degeneration （WD） remains an important research topic. Many genes are differentially expressed during the process of WD, but the precise mechanisms responsible for these differentiations are not completely understood. In this study, we used microarrays to analyze the expression changes of the distal nerve stump at 0, 1, 4, 7, 14, 21 and 28 days after sciatic nerve injury in rats. The data revealed 6 076 differentiatly-expressed genes, with 23 types of expression, specifically enriched in genes associated with nerve development and axonogenesis, cytokine biosynthesis, cell differentiation, cytokine/chemokine production, neuron differentiation, cytokinesis, phosphorylation and axon regeneration. Kyoto Encyclopedia of Genes and Genomes pathway analysis gave findings related mainly to the MAPK signaling pathway, the Jak-STAT signaling pathway, the cell cycle, cytokine-cytokine receptor interaction, the p53 signaling pathway and the Wnt signaling pathway. Some key factors were NGF, MAG, CNTF, CTNNA2, p53, JAK2, PLCB1, STAT3, BDNF, PRKC, collagen II, FGF, THBS4, TNC and c-Src, which were further validated by real-time quantitative PCR, Western blot, and immunohistochemistry. Our findings contribute to a better understanding of the functional analysis of differentially-expressed genes in WD and may shed light on the molecular mechanisms of nerve degeneration and regeneration.
基金supported by NSFC (the National Natural Science Foundation of China) [81273126, 30972454]the Key Project of Guangdong Natural Science Foundation [S2012020010903]+2 种基金the Project of Shenzhen Basic Research Plan [JCYJ20120616 154222545]the Upgrade Scheme of Shenzhen Municipal Key Laboratory [CXB201005260068A]Medical Scientific Research Foundation of Guangdong Province (A2012577)
文摘Trichloroethylene （TCE） is a major pollutant that affects both occupational and general environments. The liver is an important target organ of TCEE. Substantial efforts and remarkable progress into understanding TCE cytotoxicity have been made in cultured liver cells. However, the molecular mechanisms by which TCE induces hepatotoxicity are not well understood. SET （also known as protein phosphatase 2A inhibitor, 12PP2A, or template-activating factor-I, TAF-D is a nuclear protein that regulates histone modification, gene transcription, DNA replication, nucleosome assembly,
基金supported by the National Key Research and Development Program of China(2023YFC3603400,2022YFF0710900)the CAS Project for Young Scientists in Basic Research(YSBR-076)+8 种基金Digitalization,Development,and Application of Biotic Resource Program(202002AA100007)High-level Talent Promotion and Training Project of Kunming(Spring City Plan2020SCP001)Yunnan Revitalization Talent Support Program Yunling Scholar Project(Q.-P.K.)Yunnan Revitalization Talent Support Program Young Talent Project(G.-H.L.)Yunnan Fundamental Research Projects(202101AS070058,202201AS070080)the National Natural Science Foundation of China(82071595)Academician Expert Workstation of Yunnan Kunming(YSZJGZZ2022063)Technology Talent and Platform Plan of Yunnan Province(202305AF150160).
文摘Dear Editor,Rhesus macaques are valuable nonhuman animal models for studying the aging process,but the molecular dynamics of aging remain elusive.Although previous studies have examined gene expression changes in human and rodent aging models(Huan et al.,2018;Li et al.,2023;Schaum et al.,2020;Tumasian Iii et al.,2021),a longitudinal analysis of the aging transcriptome in non-human primates,which are evolutionarily close relatives to humans,is scarce.In this study,we conducted a time series analysis by sequencing the transcriptomes of peripheral blood from healthy female rhesus macaques,aged 7 to 29,to explore age-associated changes in gene expression(Detailed in supplementary methods).
基金supported by grants from the National Natural Science Foundation of China(81871062)Guangdong Basic and Applied Basic Research Foundation(2024A1515012913)+1 种基金the Key Research Foundation of Guangdong Provincial Education Bureau(2023ZDZX2037)the Special Fund of Science and Technology Innovation Cultivation of Guangdong University Students(pdjh2024a238,pdjh2025ak133)。
文摘Dear Editor,Post-traumatic stress disorder(PTSD)is a chronic neuropsychiatric disorder triggered by severe traumatic events,characterized by persistent intrusive memories,emotional dysregulation,hyperarousal,and avoidance behaviors[1,2].PTSD is associated with significant gene expression changes in key brain regions,including the ventral tegmental area(VTA),which may underlie dysregulation of dopaminergic signaling and stress-related behaviors[3].
基金Supported by Grants-in-Aid for Young Scientists(B)No.22791295,No.23791557 and No.24791440Grant-in-Aid for Scientific Research(C)from the Japan Society for the Promotion of Science,No.22591464 and No.24591957
文摘AIM: To investigate the role of claudin 1 in the regulation of genes involved in cell migration and tumor necrosis factor alpha (TNF-α)-induced gene expression in human gastric adenocarcinoma cells.
