Ubiquitin (UBI) plays a very important role in regulated non-lysosoma l ATP dependent protein degradation. In the present work, the coding sequence of Spodoptera litura UBI gene was isolated (GenBank Accession No. AF4...Ubiquitin (UBI) plays a very important role in regulated non-lysosoma l ATP dependent protein degradation. In the present work, the coding sequence of Spodoptera litura UBI gene was isolated (GenBank Accession No. AF436066). Th e le ngth of this ORF is 228bp, encoding a protein with Mr of 8.56 kD and isoelectri c point of 6.56. Multiple sequence alignment indicated that S.litura UBI is very similar to the homologous proteins of other eukaryotic species and it has 84% id entity with S.litura nucleopolyhedrovirus (SpltMNPV) UBI at amino acid level . RT -PCR analysis showed that S.litura UBI gene is ubiquitously expressed in la rva t issues which are susceptible to SpltMNPV infection. By constructing E.coli e xpre ssion vector, S.litura UBI was highly expressed and the recombinant protein was purified using Ni-NTA resin column, and currently further study on the function of S.litura UBI in SpltMNPV infection is underway.展开更多
RNA-binding proteins(RBPs)are key regulators in post-transcriptional control of gene expression.Mutations that alter their activity or abundance have been implicated in numerous diseases such as neurodegenerative diso...RNA-binding proteins(RBPs)are key regulators in post-transcriptional control of gene expression.Mutations that alter their activity or abundance have been implicated in numerous diseases such as neurodegenerative disorders and various types of cancer.This highlights the importance of RBP proteostasis and the necessity to tightly control the expression levels and activities of RBPs.In many cases,RBPs engage in an auto-regulatory feedback by directly binding to and influencing the fate oftheirown mRNAs,exerting control over their own expression.For this feedback control,RBPs employ a variety of mechanisms operating at all levels of posttranscriptional regulation of gene expression.Here we review RBP-mediated autogenous feedback regulation that either serves to maintain protein abundance within a physiological range(by negative feedback)or generates binary,genetic on/off switches important for e.g.cell fate decisions(by positive feedback).展开更多
文摘Ubiquitin (UBI) plays a very important role in regulated non-lysosoma l ATP dependent protein degradation. In the present work, the coding sequence of Spodoptera litura UBI gene was isolated (GenBank Accession No. AF436066). Th e le ngth of this ORF is 228bp, encoding a protein with Mr of 8.56 kD and isoelectri c point of 6.56. Multiple sequence alignment indicated that S.litura UBI is very similar to the homologous proteins of other eukaryotic species and it has 84% id entity with S.litura nucleopolyhedrovirus (SpltMNPV) UBI at amino acid level . RT -PCR analysis showed that S.litura UBI gene is ubiquitously expressed in la rva t issues which are susceptible to SpltMNPV infection. By constructing E.coli e xpre ssion vector, S.litura UBI was highly expressed and the recombinant protein was purified using Ni-NTA resin column, and currently further study on the function of S.litura UBI in SpltMNPV infection is underway.
基金This work was supported by the German Research Foundation(SFB902/2,B13 and CEF-MC to M.M.-M.,GRK 2355 to O.R.,SFB 960/2,Bll to J.M.)the German Federal Ministry of Education and Research(BMBF,01ZX1401D to J.M.)+2 种基金the LOEWE program Medical RNomics(to O.R.)the National Natural Science Foundation of China(31570820,31661143035,and 31770881 to J.H.)the National Key Research and Development Program of China(2017YFA0504400 to J.H.).
文摘RNA-binding proteins(RBPs)are key regulators in post-transcriptional control of gene expression.Mutations that alter their activity or abundance have been implicated in numerous diseases such as neurodegenerative disorders and various types of cancer.This highlights the importance of RBP proteostasis and the necessity to tightly control the expression levels and activities of RBPs.In many cases,RBPs engage in an auto-regulatory feedback by directly binding to and influencing the fate oftheirown mRNAs,exerting control over their own expression.For this feedback control,RBPs employ a variety of mechanisms operating at all levels of posttranscriptional regulation of gene expression.Here we review RBP-mediated autogenous feedback regulation that either serves to maintain protein abundance within a physiological range(by negative feedback)or generates binary,genetic on/off switches important for e.g.cell fate decisions(by positive feedback).
