Exploration of novel genetic resources against root-knot nematode(RKN)is necessary to strengthen the resistance breeding program in cultivated rice,and investigations on the role of genotype-specific root anatomy in c...Exploration of novel genetic resources against root-knot nematode(RKN)is necessary to strengthen the resistance breeding program in cultivated rice,and investigations on the role of genotype-specific root anatomy in conferring a structural barrier against nematode invasion are largely underexplored.Here,we reported a highly-resistant rice germplasm Phule Radha that conferred remarkably lower RKN parasitic fitness in terms of reduced penetration and delayed development and reproduction when compared with susceptible cultivar PB1121.Using histological and biochemical analyses,we demonstrated that an enhanced suberin deposition in the exodermal root tip tissue of Phule Radha compared to PB1121 can effectively form a penetrative barrier against RKN infection,and this preformed barrier in the control tissue did not necessarily alter to a greater extent when challenged with RKN stress.Using qRT-PCR analysis,we showed that a number of suberin biosynthesis genes were greatly expressed in the exodermis of Phule Radha compared to PB1121.In sum,the present study established the role of rice exodermal barrier system in defense against an important soil-borne pathogen.展开更多
Jatropha curcas has been expected as a biodiesel plant which can be grown in degraded lands. The structure of roots at the seedling stage, in particular cell wall modification in exodermis and endodermis, was microsco...Jatropha curcas has been expected as a biodiesel plant which can be grown in degraded lands. The structure of roots at the seedling stage, in particular cell wall modification in exodermis and endodermis, was microscopically observed. In addition, it was discussed if the first four peripheral roots that emerge from the base of the primary root (taproot) are lateral roots or adventitious roots. The primary root and the first-order lateral roots formed diarchy stele, in which two protoxylem poles present in primary xylem of root. Consequently, the first four peripheral roots cannot be lateral roots, but should be adventitious roots formed at the base of hypocotyl. In both the primary and first-order lateral roots, exodermis and endodermis formed highly lignified cell walls. Moreover, the exodermal and endodermal cell walls formed Casparian strips, which could be observed without special staining by fluorescent dye under ultraviolet microscopy. Such cell-wall modification in root exodermis and endodermis may play an important role for J. curcas under soil stresses in degraded lands.展开更多
基金supported by the grant from the Department of Biotechnology,Ministry of Science and Technology,India(Grant No.BT/PR18924/COE/34/48/2017).
文摘Exploration of novel genetic resources against root-knot nematode(RKN)is necessary to strengthen the resistance breeding program in cultivated rice,and investigations on the role of genotype-specific root anatomy in conferring a structural barrier against nematode invasion are largely underexplored.Here,we reported a highly-resistant rice germplasm Phule Radha that conferred remarkably lower RKN parasitic fitness in terms of reduced penetration and delayed development and reproduction when compared with susceptible cultivar PB1121.Using histological and biochemical analyses,we demonstrated that an enhanced suberin deposition in the exodermal root tip tissue of Phule Radha compared to PB1121 can effectively form a penetrative barrier against RKN infection,and this preformed barrier in the control tissue did not necessarily alter to a greater extent when challenged with RKN stress.Using qRT-PCR analysis,we showed that a number of suberin biosynthesis genes were greatly expressed in the exodermis of Phule Radha compared to PB1121.In sum,the present study established the role of rice exodermal barrier system in defense against an important soil-borne pathogen.
文摘Jatropha curcas has been expected as a biodiesel plant which can be grown in degraded lands. The structure of roots at the seedling stage, in particular cell wall modification in exodermis and endodermis, was microscopically observed. In addition, it was discussed if the first four peripheral roots that emerge from the base of the primary root (taproot) are lateral roots or adventitious roots. The primary root and the first-order lateral roots formed diarchy stele, in which two protoxylem poles present in primary xylem of root. Consequently, the first four peripheral roots cannot be lateral roots, but should be adventitious roots formed at the base of hypocotyl. In both the primary and first-order lateral roots, exodermis and endodermis formed highly lignified cell walls. Moreover, the exodermal and endodermal cell walls formed Casparian strips, which could be observed without special staining by fluorescent dye under ultraviolet microscopy. Such cell-wall modification in root exodermis and endodermis may play an important role for J. curcas under soil stresses in degraded lands.
