Background:Si-Jun-Zi-Tang(SJZT),a traditional Chinese herbal formula,is designed to boost vitality and promote spleen health,bringing numerous benefits to the body.It has indicated that herbal remedies have a potentia...Background:Si-Jun-Zi-Tang(SJZT),a traditional Chinese herbal formula,is designed to boost vitality and promote spleen health,bringing numerous benefits to the body.It has indicated that herbal remedies have a potential link with enhanced exercise performance,reducing the risk of various chronic diseases.In this study,we will observe the impact of SJZT on the exercise performance of mice and explore the underlying mechanism.Methods:In this study,we used the steam distillation method to obtain the extract of SJZT.Mice were given pure water,low and high doses of SJZT respectively for 4 weeks,followed by the assessment of exercise performance(Forelimb Grip Strength Test,Exhausted Swimming Training,Exhausted Treadmill Running Test,and 10-min Swimming Test).Subsequently,serum and urine biochemical indices,glycogen content,liver mRNA,and protein expression were analyzed after the mice completed 6 weeks of treadmill training.In the cellular experiments,we used the HepG2 cell line treated with SJZT post-gavage serum to mimic the in-vivo SJZT-administered mouse model,and used Western blot,qPCR,and other experimental techniques to thoroughly investigate the potential mechanism of SJZT to enhance exercise performance.Results:Compared with the Vehicle group,the SJZT groups showed increased lactate clearance and elevated liver glycogen levels.In terms of exercise performance,grip strength,exercise capacity,and lactate clearance were increased,and lactate production was decreased in the SJZT groups.In comparison to the Vehicle group,the SJZT groups exhibited decreased RNA and protein expression of p-p38 MAPK,increased RNA and protein expression of SIRT1 and PGC-1α,elevated protein expression of p-AMPK,and reduced protein expression of NF-κB.Consistent with the in-vivo results,the in-vitro results indicated that SJZT improved exercise performance by modulating p38 MAPK signaling.Conclusion:These results suggest that SJZT improves exercise performance in mice by a mechanism that may be related to the inhibition of the p38 MAPK signaling pathway.展开更多
Physical performance is determined both by biophysical and physiological limitations and behav- ioral characteristic, specifically motivation. We applied an experimental evolution approach com- bined with pharmacologi...Physical performance is determined both by biophysical and physiological limitations and behav- ioral characteristic, specifically motivation. We applied an experimental evolution approach com- bined with pharmacological manipulation to test the hypothesis that evolution of increased aerobic exercise performance can be triggered by evolution of motivation to undertake physical activity. We used a unique model system： bank voles from A lines, selected for high swim-induced aerobic metabolism （VO2swim）, which achieved a 61% higher mass-adjusted VO2swim than those from un- selected C lines. Because the voles could float on the water surface with only a minimum activity, the maximum rate of metabolism achieved in that test depended not only on their aerobic capacity, but also on motivation to undertake intensive activity. Therefore, we hypothesized that signaling of neurotransmitters putatively involved in regulating physical activity （dopamine and noradrenaline） had changed in response to selection. We measured VO2swim after intraperitoneal injections of sa- line or the norepinephrine and dopamine reuptake inhibitor bupropion （20 mg/kg or 30 mg/kg）. Additionally, we measured forced-exercise VO2 （VO2max）. In C lines, VO2swim （mass-adjusted mean ± standard error （SE）： 4.0 ± 0.1 mLO2/min） was lower than VO2max （5.0 ± 0.1 mLO2/min）, but in A lines VO2swim （6.0 ± 0.1 mLO2/min） was as high as VO2max （6.0 ± 0.1 mLO2/min）. Thus, the selection effectively changed both the physiological-physical performance limit and mechanisms responsible for the willingness to undertake vigorous locomotor activity. Surprisingly, the drug had no effect on the achieved level of VO2swim. Thus, the results did not allow firm conclusions concerning involvement of these neurotransmitters in evolution of increased aerobic exercise performance in the experimental evolution model system.展开更多
Objectives:Aerobic exercise training can increase skeletal muscle mitochondrial content.Supine exercise training with legs above the heart potentially augments these increases.However,the impact of supine exercise tra...Objectives:Aerobic exercise training can increase skeletal muscle mitochondrial content.Supine exercise training with legs above the heart potentially augments these increases.However,the impact of supine exercise training on mitochondrial biogenesis and cardiovascular adaptations remains unclear.Methods:In this single-centred,randomized,parallel arm trial,19 recreationally active individuals underwent seven sessions of either supine with legs up(SUP;n=9,6 females)or upright with legs down(UP;n=10,7 females)aerobic training on a recumbent bike at 71±7%and 71±2%of peak work rate(WRpeak),respectively.The study aimed to test the effects of training with decreased muscle oxygenation on indices of muscle mitochondrial remodelling.Secondary outcomes included exercise performance,muscle oxygenation,and cardiovascular responses.Results:Secondary outcomes revealed significant interaction effects for time to fatigue(TTF)and WRpeak in the SUP group during supine testing,suggesting enhanced exercise tolerance and performance.No between group interaction effects were observed for upright testing.No clear effects on mitochondrial biogenesis were observed based on expression of mitochondrial protein subunits and transcriptional regulators.Acutely,HRpeak was lower during the SUP Test compared to the UP Test.No central cardiovascular adaptations were observed following training.Conclusions:Our exploratory analyses showed that supine aerobic training more effectively improves supine exercise tolerance and performance compared with upright training,despite no differences in measured proteins related to mitochondrial biogenesis.Further research is needed to elucidate the mechanisms underlying these postural-specific training effects.Registration:clinicaltrials.gov:NCT04151095.展开更多
The gut microbiota refers to the collection of trillions of intestinal microorganisms that modulate central aspects of health and disease through influential effects on host physiology.Recently,a connection has been m...The gut microbiota refers to the collection of trillions of intestinal microorganisms that modulate central aspects of health and disease through influential effects on host physiology.Recently,a connection has been made between the gut microbiota and exercise.Initial investigations demonstrated the beneficial effects of exercise on the gut microbiota,with cross-sectional studies revealing positive correlations between exerciseassociated states,and healthy gut microbiota and exercise interventions showed post-intervention increases in the abundance of beneficial bacterial taxa.More recent investigations have focused on exploring the reverse relationship:the influence of the gut microbiota on exercise performance.Murine investigations have revealed that certain bacterial taxa may enhance endurance exercise performance by augmenting various aspects of lactate metabolism.Further,short-chain fatty acids—which modulate metabolism at various organ sites,including within skeletal muscle—have been shown to enhance endurance exercise capacity in mice.This review highlights what is currently known about the connection between the gut microbiota and exercise,with a particular focus on the ergogenic potential of the gut microbiota and how it may be leveraged to enhance endurance exercise performance.展开更多
Youth athletes are ideal candidates for novel therapeutic motor learning interventions that leverage the plasticity of the central nervous system to promote desirable biomechanical adaptions.We summarize the empirical...Youth athletes are ideal candidates for novel therapeutic motor learning interventions that leverage the plasticity of the central nervous system to promote desirable biomechanical adaptions.We summarize the empirical data supporting the three pillars of the Optimizing Performance Through Intrinsic Motivation and Attention for Learning(OPTIMAL)theory of motor learning and expand on potential neurophysiologic mechanisms that will support enhanced movement mechan-ics in youth to optimize prevention programs for reduced injury risk,injury rehabilitation,exercise performance,and play(Prevention Rehabilitation Exercise Play;PREP).Specifically,we highlight the role of motivational factors to promote the release of dopamine that could accelerate motor performance and learning adaptations.Further,we detail the potential for an external focus of attention to shift attentional allocation and increase brain activity in regions important for sensorimotor integration to facilitate primary motor cortex efficiency.This manuscript serves to provide the most current data in support of the application of OPTIMAL PREP training strategies of the future.展开更多
Purpose The safety of creatine supplementation has been investigated,however,the safety of a high-dose/short-term or low-dose/long-term supplementation dosing scheme,combined with a resistance training program,a commo...Purpose The safety of creatine supplementation has been investigated,however,the safety of a high-dose/short-term or low-dose/long-term supplementation dosing scheme,combined with a resistance training program,a commonly used dosing scheme,has not yet studied.The aim of this study was to evaluate physical performance and the safety of the cited creatine supplementation dosing scheme in a double-blind placebo-controlled study by assessing blood and urine health indicators in subjects undertaking resistance training.Method Thirty-four healthy male subjects were randomly assigned to creatine supplement(CREA/7 days,0.3 g/kg body mass+21 days,0.03 g/kg)or placebo(PLA)group and both groups participated in a resistance training intervention(6 exercises,3 sets of 8-12 repetitions per exercise at 85%of 1RM,3 d/week).Blood and urine samples were collected pre-,7 and 30 days post-supplementation.For blood(26 parameters)and urine markers(15),between-group differences were calculated by using 2 groups(CREA and PLA)×2 measures(“POST7 minus PRE”and“POST30 minus PRE”)terms.Results The CREA group exhibited significant body weight and 1RM increase(POST30 minus PRE)of all evaluated exercises,when compared to PLA group.Regarding the blood and urine health markers,only hematocrit,LDL,Uric Acid,Phosphatase Alkaline and Creatinine exhibited between-group differences,but with small magnitude of differences and maintained into the range of clinical reference values.Conclusion Our data suggest that creatine supplementation the used dosing scheme,concomitantly with resistance training,improves physical performance with no evident risk or protection to health for young weightlifters.展开更多
Youth may be particularly responsive to motor learning training strategies that support injury-resistant movement mechanics in youth for prevention programs that reduce injury risk,injury rehabilitation,exercise perfo...Youth may be particularly responsive to motor learning training strategies that support injury-resistant movement mechanics in youth for prevention programs that reduce injury risk,injury rehabilitation,exercise performance,and play more gener-ally(Optimizing Performance Through Intrinsic Motivation and Attention for Learning Prevention Rehabilitation Exercise Play;OPTIMAL PREP)One purpose of the present manuscript was to provide clinical applications and tangible examples of how to implement the proposed techniques derived from OPTIMAL theory into PREP strategies for youth.A secondary purpose was to review recent advances in technology that support the clinical application of OPTIMAL PREP strategies without extensive resources/programming knowledge to promote evidence-driven tools that will support practitioner feedback delivery.The majority of examples provided are within the context of anterior cruciate ligament(ACL)injury rehabilitation,but we emphasize the potential for OPTIMAL PREP strategies to be applied to a range of populations and training scenarios that will promote injury resistance and keep youth active and healthy.展开更多
基金supported by Basic Scientific Research Fund of Education Department of Liaoning Province,China(LJ212410161100 to Z-L.L.)Natural Science Foundation of Liaoning Province,China(2022-MS-326 to Z-L.L.and 2021-BS-294 to B.W.)+1 种基金Dengfeng project of Dalian medical discipline priority(2022ZZ258 and 2023ZZ025 to B.W.)China Foundation For Youth Entrepreneurship And Employment(P24041687730 to L-N.X.).
文摘Background:Si-Jun-Zi-Tang(SJZT),a traditional Chinese herbal formula,is designed to boost vitality and promote spleen health,bringing numerous benefits to the body.It has indicated that herbal remedies have a potential link with enhanced exercise performance,reducing the risk of various chronic diseases.In this study,we will observe the impact of SJZT on the exercise performance of mice and explore the underlying mechanism.Methods:In this study,we used the steam distillation method to obtain the extract of SJZT.Mice were given pure water,low and high doses of SJZT respectively for 4 weeks,followed by the assessment of exercise performance(Forelimb Grip Strength Test,Exhausted Swimming Training,Exhausted Treadmill Running Test,and 10-min Swimming Test).Subsequently,serum and urine biochemical indices,glycogen content,liver mRNA,and protein expression were analyzed after the mice completed 6 weeks of treadmill training.In the cellular experiments,we used the HepG2 cell line treated with SJZT post-gavage serum to mimic the in-vivo SJZT-administered mouse model,and used Western blot,qPCR,and other experimental techniques to thoroughly investigate the potential mechanism of SJZT to enhance exercise performance.Results:Compared with the Vehicle group,the SJZT groups showed increased lactate clearance and elevated liver glycogen levels.In terms of exercise performance,grip strength,exercise capacity,and lactate clearance were increased,and lactate production was decreased in the SJZT groups.In comparison to the Vehicle group,the SJZT groups exhibited decreased RNA and protein expression of p-p38 MAPK,increased RNA and protein expression of SIRT1 and PGC-1α,elevated protein expression of p-AMPK,and reduced protein expression of NF-κB.Consistent with the in-vivo results,the in-vitro results indicated that SJZT improved exercise performance by modulating p38 MAPK signaling.Conclusion:These results suggest that SJZT improves exercise performance in mice by a mechanism that may be related to the inhibition of the p38 MAPK signaling pathway.
文摘Physical performance is determined both by biophysical and physiological limitations and behav- ioral characteristic, specifically motivation. We applied an experimental evolution approach com- bined with pharmacological manipulation to test the hypothesis that evolution of increased aerobic exercise performance can be triggered by evolution of motivation to undertake physical activity. We used a unique model system： bank voles from A lines, selected for high swim-induced aerobic metabolism （VO2swim）, which achieved a 61% higher mass-adjusted VO2swim than those from un- selected C lines. Because the voles could float on the water surface with only a minimum activity, the maximum rate of metabolism achieved in that test depended not only on their aerobic capacity, but also on motivation to undertake intensive activity. Therefore, we hypothesized that signaling of neurotransmitters putatively involved in regulating physical activity （dopamine and noradrenaline） had changed in response to selection. We measured VO2swim after intraperitoneal injections of sa- line or the norepinephrine and dopamine reuptake inhibitor bupropion （20 mg/kg or 30 mg/kg）. Additionally, we measured forced-exercise VO2 （VO2max）. In C lines, VO2swim （mass-adjusted mean ± standard error （SE）： 4.0 ± 0.1 mLO2/min） was lower than VO2max （5.0 ± 0.1 mLO2/min）, but in A lines VO2swim （6.0 ± 0.1 mLO2/min） was as high as VO2max （6.0 ± 0.1 mLO2/min）. Thus, the selection effectively changed both the physiological-physical performance limit and mechanisms responsible for the willingness to undertake vigorous locomotor activity. Surprisingly, the drug had no effect on the achieved level of VO2swim. Thus, the results did not allow firm conclusions concerning involvement of these neurotransmitters in evolution of increased aerobic exercise performance in the experimental evolution model system.
基金supported by funding provided to BJG from the Natural Sciences and Engineering Research Council of Canada(NSERCgrant no.402635).
文摘Objectives:Aerobic exercise training can increase skeletal muscle mitochondrial content.Supine exercise training with legs above the heart potentially augments these increases.However,the impact of supine exercise training on mitochondrial biogenesis and cardiovascular adaptations remains unclear.Methods:In this single-centred,randomized,parallel arm trial,19 recreationally active individuals underwent seven sessions of either supine with legs up(SUP;n=9,6 females)or upright with legs down(UP;n=10,7 females)aerobic training on a recumbent bike at 71±7%and 71±2%of peak work rate(WRpeak),respectively.The study aimed to test the effects of training with decreased muscle oxygenation on indices of muscle mitochondrial remodelling.Secondary outcomes included exercise performance,muscle oxygenation,and cardiovascular responses.Results:Secondary outcomes revealed significant interaction effects for time to fatigue(TTF)and WRpeak in the SUP group during supine testing,suggesting enhanced exercise tolerance and performance.No between group interaction effects were observed for upright testing.No clear effects on mitochondrial biogenesis were observed based on expression of mitochondrial protein subunits and transcriptional regulators.Acutely,HRpeak was lower during the SUP Test compared to the UP Test.No central cardiovascular adaptations were observed following training.Conclusions:Our exploratory analyses showed that supine aerobic training more effectively improves supine exercise tolerance and performance compared with upright training,despite no differences in measured proteins related to mitochondrial biogenesis.Further research is needed to elucidate the mechanisms underlying these postural-specific training effects.Registration:clinicaltrials.gov:NCT04151095.
基金supported by a Canadian Institutes of Health Research Grant(PJT-159626).
文摘The gut microbiota refers to the collection of trillions of intestinal microorganisms that modulate central aspects of health and disease through influential effects on host physiology.Recently,a connection has been made between the gut microbiota and exercise.Initial investigations demonstrated the beneficial effects of exercise on the gut microbiota,with cross-sectional studies revealing positive correlations between exerciseassociated states,and healthy gut microbiota and exercise interventions showed post-intervention increases in the abundance of beneficial bacterial taxa.More recent investigations have focused on exploring the reverse relationship:the influence of the gut microbiota on exercise performance.Murine investigations have revealed that certain bacterial taxa may enhance endurance exercise performance by augmenting various aspects of lactate metabolism.Further,short-chain fatty acids—which modulate metabolism at various organ sites,including within skeletal muscle—have been shown to enhance endurance exercise capacity in mice.This review highlights what is currently known about the connection between the gut microbiota and exercise,with a particular focus on the ergogenic potential of the gut microbiota and how it may be leveraged to enhance endurance exercise performance.
文摘Youth athletes are ideal candidates for novel therapeutic motor learning interventions that leverage the plasticity of the central nervous system to promote desirable biomechanical adaptions.We summarize the empirical data supporting the three pillars of the Optimizing Performance Through Intrinsic Motivation and Attention for Learning(OPTIMAL)theory of motor learning and expand on potential neurophysiologic mechanisms that will support enhanced movement mechan-ics in youth to optimize prevention programs for reduced injury risk,injury rehabilitation,exercise performance,and play(Prevention Rehabilitation Exercise Play;PREP).Specifically,we highlight the role of motivational factors to promote the release of dopamine that could accelerate motor performance and learning adaptations.Further,we detail the potential for an external focus of attention to shift attentional allocation and increase brain activity in regions important for sensorimotor integration to facilitate primary motor cortex efficiency.This manuscript serves to provide the most current data in support of the application of OPTIMAL PREP training strategies of the future.
文摘Purpose The safety of creatine supplementation has been investigated,however,the safety of a high-dose/short-term or low-dose/long-term supplementation dosing scheme,combined with a resistance training program,a commonly used dosing scheme,has not yet studied.The aim of this study was to evaluate physical performance and the safety of the cited creatine supplementation dosing scheme in a double-blind placebo-controlled study by assessing blood and urine health indicators in subjects undertaking resistance training.Method Thirty-four healthy male subjects were randomly assigned to creatine supplement(CREA/7 days,0.3 g/kg body mass+21 days,0.03 g/kg)or placebo(PLA)group and both groups participated in a resistance training intervention(6 exercises,3 sets of 8-12 repetitions per exercise at 85%of 1RM,3 d/week).Blood and urine samples were collected pre-,7 and 30 days post-supplementation.For blood(26 parameters)and urine markers(15),between-group differences were calculated by using 2 groups(CREA and PLA)×2 measures(“POST7 minus PRE”and“POST30 minus PRE”)terms.Results The CREA group exhibited significant body weight and 1RM increase(POST30 minus PRE)of all evaluated exercises,when compared to PLA group.Regarding the blood and urine health markers,only hematocrit,LDL,Uric Acid,Phosphatase Alkaline and Creatinine exhibited between-group differences,but with small magnitude of differences and maintained into the range of clinical reference values.Conclusion Our data suggest that creatine supplementation the used dosing scheme,concomitantly with resistance training,improves physical performance with no evident risk or protection to health for young weightlifters.
文摘Youth may be particularly responsive to motor learning training strategies that support injury-resistant movement mechanics in youth for prevention programs that reduce injury risk,injury rehabilitation,exercise performance,and play more gener-ally(Optimizing Performance Through Intrinsic Motivation and Attention for Learning Prevention Rehabilitation Exercise Play;OPTIMAL PREP)One purpose of the present manuscript was to provide clinical applications and tangible examples of how to implement the proposed techniques derived from OPTIMAL theory into PREP strategies for youth.A secondary purpose was to review recent advances in technology that support the clinical application of OPTIMAL PREP strategies without extensive resources/programming knowledge to promote evidence-driven tools that will support practitioner feedback delivery.The majority of examples provided are within the context of anterior cruciate ligament(ACL)injury rehabilitation,but we emphasize the potential for OPTIMAL PREP strategies to be applied to a range of populations and training scenarios that will promote injury resistance and keep youth active and healthy.
