This study presents a comprehensive phylogenetic analysis on Batrachospermaceae based on key taxonomic identifiers(rbcL,psaA,psbA,and COI-5P)from some genera.To systematically explore the phylogenetic relationships an...This study presents a comprehensive phylogenetic analysis on Batrachospermaceae based on key taxonomic identifiers(rbcL,psaA,psbA,and COI-5P)from some genera.To systematically explore the phylogenetic relationships and taxonomy within Batrachospermaceae,we integrated molecular and morphological data,and explored the phylogeny,character evolution,and ancestral geographical origin and provided a theoretical support for the classification and geographic origination of Batrachospermaceae.Our findings reveal distinct relationships within the phylogenetic tree.Notably,10 genera(Sirodotia,Batrachospermum,Tuomeya,Volatus,Lympha,Nothocladus,Torularia,Sheathia,Nocturama,and Petrohua)are closely associated in the rbcL phylogenetic tree.Additionally,four genera(Kumanoa,Hoefkenia,Notohesperus,and Virescentia)exhibit high support ratios,indicating their close interrelations.Other genera,including Paludicola,Visia,Acarposporophycos,Macrosporophycos,Visioidea,Balliopsis,and Psilosiphon,exhibit clustering traits.Furthermore,the multigene sequences provide a robust support for Montagnia that forms a monophyletic group.Ancestral reconstruction of morphological characters identifies nine primitive character states,including whorl,fascicle length,cortical cells,secondary fascicles,the shape of carpogonical branch,spermatangia,carposporophyte,carpogonium and trichogyne,with Visia likely representing ancestral traits in Batrachospermaceae.Furthermore,geographical origin maps suggest a potential common ancestral of Batrachospermaceae origin in the American continent.Additional to conventional analyses,including evolutionary and ancestral reconstruction investigations into key morphological characters,we attempt to reconstruct the biogeography within the Batrachospermaceae,thus contributing to a nuanced understanding of its origin.展开更多
NiMo-based catalysts show significant potential for the hydrogen evolution reaction(HER).Optimizing the electronic structure and enhancing mass transfer are two critical factors for improving catalytic performance,but...NiMo-based catalysts show significant potential for the hydrogen evolution reaction(HER).Optimizing the electronic structure and enhancing mass transfer are two critical factors for improving catalytic performance,but they remain significant challenges.Herein,we present a route for synthesizing two-dimensional(2D)porous Mo_(2)N-Ni heterojunction nanosheets with tuned Ni-Mo ratio for enhanced alkaline HER performance.A precursor can be easily synthesized by assembling polyoxometalate clusters(PMo_(12))with layered hydroxy oxides(Ni(OH)_(2)).It is found that the interaction between PMo_(12)and Ni(OH)_(2)can effectively protect the particles from significant agglomeration during pyrolysis,resulting in the formation of 2D porous sheets composed of small Mo_(2)N-Ni units.The transfer of electrons from Ni to Mo_(2)N results in the redistribution of electrons at the heterojunction,optimizing the adsorption and desorption of intermediates.Moreover,the 2D porous structure comprised of small particles enhances mass transfer,thereby reducing the impedance of the catalyst.Consequently,the catalyst with an optimized Mo/Ni ratio exhibits an overpotential of 19 mV at 10 mA cm^(-2),being comparable to that of commercial Pt/C catalyst.The anion exchange membrane(AEM)electrolyzer,consisting of optimized Mo_(2)N-Ni and NiFe-LDH,achieves a current density of 500 mA cm^(-2)at 1.80 V and can operate stably for 300 h.This assembly method offers an effective strategy for the large-scale preparation of efficient catalysts.展开更多
Portschinskia Semenov is a rare genus of bot fies whose larvae are obligate parasites of pikas and murine rodents,crucial for understanding the evolutionary biology of Oestridae.However,limited information on their ad...Portschinskia Semenov is a rare genus of bot fies whose larvae are obligate parasites of pikas and murine rodents,crucial for understanding the evolutionary biology of Oestridae.However,limited information on their adult biology and early immature stages has hindered the progress.Here,we provided the frst documentation of adult oviposition,behavior,and morphology of newly hatched frst instars of P.magnifca Pleske.Using confocal laser scanning microscopy and scanning electron microscopy,we characterized the ultrastructure of egg and frst-instar larva,identifying key traits facilitating attachment.Eggs were deposited individually or in groups,and glued on non-host surfaces with white adhesive substances,without specialized attachment organ.Newly hatched frst instars were circled with an anterior spinose band on each body segment except the anal division,awaited hosts in an upright position,and anchored to the egg shell by their anal division,likely supported by curved spines originating from the peritreme of the posterior spiracles.Evolutionary analyses of reproductive behavior across the 4 bot fy subfamilies reveal at least 3 times independent evolution of oviposition on non-host surfaces in Portschinskia or the Hypodermatinae clade as a sister group to Ochotonia,Gasterophilus pecorum(Fabricius)(Gasterophilinae),and Cuterebrinae.In contrast,species in the Oestrinae are larviparous,depositing frst instars directly onto hosts.Our fndings shed light on oviposition behavior and early immature morphology of the rare genus Portschinskia,offering insights into reproductive strategies and evolutionary adaptations of bot fies.展开更多
Chromosomal rearrangements(CRs)often cause phenotypic variations.Although several major rearrangements have been identified in Triticeae,a comprehensive study of the order,timing,and breakpoints of CRs has not been co...Chromosomal rearrangements(CRs)often cause phenotypic variations.Although several major rearrangements have been identified in Triticeae,a comprehensive study of the order,timing,and breakpoints of CRs has not been conducted.Here,we reconstruct high-quality ancestral genomes for the most recent common ancestor(MRCA)of the Triticeae,and the MRCA of the wheat lineage(Triticum and Aegilops).The protogenes of MRCA of the Triticeae and the wheat lineage are 22,894 and 29,060,respectively,which were arranged in their ancestral order.By partitioning modern Triticeae chromosomes into sets of syntenic regions and linking each to the corresponding protochromosomes,we revisit the rye chromosome structural evolution and propose alternative evolutionary routes.The previously identified 4L/5L reciprocal translocation in rye and Triticum urartu is found to have occurred independently and is unlikely to be the result of chromosomal introgression following distant hybridization.We also clarify that the 4AL/7BS translocation in tetraploid wheat was a bidirectional rather than unidirectional translocation event.Lastly,we identify several breakpoints in protochromosomes that independently reoccur following Triticeae evolution,representing potential CR hotspots.This study demonstrates that these reconstructed ancestral genomes can serve as special comparative references and facilitate a better understanding of the evolution of structural rearrangements in Triticeae.展开更多
Saccharomyces cerevisiae is not naturally capable of efficiently utilizing xylose as a carbon source.When cultured with lignocellulosic hydrolysates containing pretreatment-derived inhibitors,S.cerevisiae suffers from...Saccharomyces cerevisiae is not naturally capable of efficiently utilizing xylose as a carbon source.When cultured with lignocellulosic hydrolysates containing pretreatment-derived inhibitors,S.cerevisiae suffers from much lower sugar uptake,ethanol yield and fermentation efficiency.Thus,considering efficient xylose conversion into ethanol during non-detoxified hydrolysate culture,genetic engineering and adaptive evolution of S.cerevisiae might be a promising joint strategy for improving xylose uptake and ethanol production.In this study,an inhibitor-tolerant strain S.cerevisiae SPSC01-TAF94 was genetically engineered by overexpressing both xylose transport-and metabolism-related genes(N360F,Ru-xyl A,TAL1,TKL1,RKI1 and RPE1),yielding the xylose-utilizing strain TAF94-X,followed by three-stage adaptation in non-detoxified corn stover hydrolysate containing 5 g·L^(-1)acetic acid,0.32 g·L^(-1)furfural,0.17 g·L(-1)HMF and 0.19 g·L^(-1)vanillin as the major inhibitors as well as 20,40 and 60 g·L^(-1)xylose adjusted as the major carbon source,respectively.Finally,an active xylose-utilizing and ethanolproducing strain TAF94-X60 was obtained,which achieved 44.9 g·L^(-1)ethanol with yield of0.41 g·g^(-1),productivity of 0.62 g·L^(-1)·h^(-1)and xylose consumption rate of 0.42 g·L^(-1)·h^(-1)during hydrolysate culture,compared to those of 36.5 g·L^(-1),0.38 g·g^(-1),0.50 g·L^(-1)·h^(-1)and 0.20 g·L^(-1)·h^(-1)obtained with the control strain TAF94-X.The proposed joint strategy effectively utilizes hydrolyzed sugars while eliminating the need for conventional detoxification or water washing processes,thus enhancing the economic feasibility of large-scale lignocellulosic ethanol production.展开更多
The Malvaceae family,the most diverse family in the order Malvales,consists of nine subfamilies.Within the Firmiana genus of the Sterculioideae subfamily,most species are considered globally vulnerable,yet their genom...The Malvaceae family,the most diverse family in the order Malvales,consists of nine subfamilies.Within the Firmiana genus of the Sterculioideae subfamily,most species are considered globally vulnerable,yet their genomes remain unexplored.Here,we present a chromosome-level genome assembly for a representative Firmiana species,F.hainanensis,2n=40,totaling 1536 Mb.Phylogenomic analysis shows that F.hainanensis and Durio zibethinus have the closest evolutionary relationship,with an estimated divergence time of approximately 21 millions of years ago(MYA)and distinct polyploidization events in their histories.Evolutionary trajectory analyses indicate that fissions and fusions may play a crucial role in chromosome number variation(2n=14 to 2n=96).Analysis of repetitive elements among Malvaceae reveals that the Tekay subfamily(belonging to the Gypsy group)contributes to variation in genome size(ranging from 324 Mb to 1620 Mb).Additionally,genes associated with P450,peroxidase,and microtubules,and thereby related to cell wall biosynthesis,are significantly contracted in F.hainanensis,potentially leading to its lower wood density relative to Hopea hainanensis.Overall,our study provides insights into the evolution of chromosome number,genome size,and the genetic basis of cell wall biosynthesis in Malvaceae species.展开更多
A composite electrocatalyst,CoMoNiO-S/NF-110(NF is nickel foam),was synthesized through electrodeposition,followed by pyrolysis and then the vulcanization process.CoMoNiO-S/NF-110 exhibited a structure where Ni3S2 and...A composite electrocatalyst,CoMoNiO-S/NF-110(NF is nickel foam),was synthesized through electrodeposition,followed by pyrolysis and then the vulcanization process.CoMoNiO-S/NF-110 exhibited a structure where Ni3S2 and Mo2S3 nanoparticles were integrated at the edges of Co3O4 nanosheets,creating a rich,heterogeneous interface that enhances the synergistic effects of each component.In an alkaline electrolyte,the synthesized CoMoNiO-S/NF-110 exhibited superior electrocatalytic performance for oxygen evolution reaction(OER),achieving current densities of 100 and 200 mA·cm^(-2) with low overpotentials of 199.4 and 224.4 mV,respectively,outperforming RuO2 and several high-performance Mo and Ni-based catalysts.This excellent performance is attributed to the rich interface formed between the components and active sites exposed by the defect structure.展开更多
The fungal order Botryosphaeriales includes numerous ecologically and economically important plant-associated taxa,yet its genomic diversity and evolutionary mechanisms remain poorly understood.Here,we present high-qu...The fungal order Botryosphaeriales includes numerous ecologically and economically important plant-associated taxa,yet its genomic diversity and evolutionary mechanisms remain poorly understood.Here,we present high-quality de novo genome assemblies for three representative species—Botryosphaeria dothidea,Neofusicoccum parvum,and Phyllosticta capitalensis—and perform integrative analyses using comparative genomics,population genetics,and pan-genome frameworks.Pathogenic species(B.dothidea and N.parvum)exhibit significant expansions in gene families related to membrane transport and metabolism,suggesting enhanced adaptability and virulence potential.Selective sweep analyses highlight population-level divergence in metabolic and stress-response pathways,reflecting natural selection in host and environmental adaptation.Cross-species pan-genome comparisons of six Phyllosticta species reveal a conserved core genome,dynamic gene family turnover,and extensive horizontal gene transfer from bacterial,and archaeal sources—potentially driving ecological diversification.Furthermore,effector proteins display striking domain variation across genera,particularly in regions associated with host cell wall targeting,indicating convergent strategies for host adaptation.Together,these findings provide comprehensive insights into the genomic evolution,adaptation,and virulence mechanisms of Botryosphaeriales fungi,laying a foundation for future studies on plant–fungal interactions.展开更多
The poor electrical conductivity of metal-organic frameworks(MOFs)limits their electrocatalytic performance in the oxygen evolution reaction(OER).In this study,a Py@Co-MOF composite material based on pyrene(Py)molecul...The poor electrical conductivity of metal-organic frameworks(MOFs)limits their electrocatalytic performance in the oxygen evolution reaction(OER).In this study,a Py@Co-MOF composite material based on pyrene(Py)molecules and{[Co2(BINDI)(DMA)_(2)]·DMA}_(n)(Co-MOF,H4BINDI=N,N'-bis(5-isophthalic acid)naphthalenediimide,DMA=N,N-dimethylacetamide)was synthesized via a one-pot method,leveragingπ-πinteractions between pyrene and Co-MOF to modulate electrical conductivity.Results demonstrate that the Py@Co-MOF catalyst exhibited significantly enhanced OER performance compared to pure Co-MOF or pyrene-based electrodes,achieving an overpotential of 246 mV at a current density of 10 mA·cm^(-2) along with excellent stability.Density functional theory(DFT)calculations reveal that the formation of O*in the second step is the rate-determining step(RDS)during the OER process on Co-MOF,with an energy barrier of 0.85 eV due to the weak adsorption affinity of the OH*intermediate for Co sites.CCDC:2419276.展开更多
基金Supported by the National Natural Science Foundation of China(No.32170204)。
文摘This study presents a comprehensive phylogenetic analysis on Batrachospermaceae based on key taxonomic identifiers(rbcL,psaA,psbA,and COI-5P)from some genera.To systematically explore the phylogenetic relationships and taxonomy within Batrachospermaceae,we integrated molecular and morphological data,and explored the phylogeny,character evolution,and ancestral geographical origin and provided a theoretical support for the classification and geographic origination of Batrachospermaceae.Our findings reveal distinct relationships within the phylogenetic tree.Notably,10 genera(Sirodotia,Batrachospermum,Tuomeya,Volatus,Lympha,Nothocladus,Torularia,Sheathia,Nocturama,and Petrohua)are closely associated in the rbcL phylogenetic tree.Additionally,four genera(Kumanoa,Hoefkenia,Notohesperus,and Virescentia)exhibit high support ratios,indicating their close interrelations.Other genera,including Paludicola,Visia,Acarposporophycos,Macrosporophycos,Visioidea,Balliopsis,and Psilosiphon,exhibit clustering traits.Furthermore,the multigene sequences provide a robust support for Montagnia that forms a monophyletic group.Ancestral reconstruction of morphological characters identifies nine primitive character states,including whorl,fascicle length,cortical cells,secondary fascicles,the shape of carpogonical branch,spermatangia,carposporophyte,carpogonium and trichogyne,with Visia likely representing ancestral traits in Batrachospermaceae.Furthermore,geographical origin maps suggest a potential common ancestral of Batrachospermaceae origin in the American continent.Additional to conventional analyses,including evolutionary and ancestral reconstruction investigations into key morphological characters,we attempt to reconstruct the biogeography within the Batrachospermaceae,thus contributing to a nuanced understanding of its origin.
基金supported by the National Key R&D Program of China(2022YFA1503002,2022YFA1503003)the National Natural Science Foundation of China(22271081)+2 种基金the Natural Science Foundation of Heilongjiang Province(PL2024B017)the Postdoctoral Science Foundation of Heilongjiang Province(LBH-Z22240)the Heilongjiang University Excellent Youth Foundation。
文摘NiMo-based catalysts show significant potential for the hydrogen evolution reaction(HER).Optimizing the electronic structure and enhancing mass transfer are two critical factors for improving catalytic performance,but they remain significant challenges.Herein,we present a route for synthesizing two-dimensional(2D)porous Mo_(2)N-Ni heterojunction nanosheets with tuned Ni-Mo ratio for enhanced alkaline HER performance.A precursor can be easily synthesized by assembling polyoxometalate clusters(PMo_(12))with layered hydroxy oxides(Ni(OH)_(2)).It is found that the interaction between PMo_(12)and Ni(OH)_(2)can effectively protect the particles from significant agglomeration during pyrolysis,resulting in the formation of 2D porous sheets composed of small Mo_(2)N-Ni units.The transfer of electrons from Ni to Mo_(2)N results in the redistribution of electrons at the heterojunction,optimizing the adsorption and desorption of intermediates.Moreover,the 2D porous structure comprised of small particles enhances mass transfer,thereby reducing the impedance of the catalyst.Consequently,the catalyst with an optimized Mo/Ni ratio exhibits an overpotential of 19 mV at 10 mA cm^(-2),being comparable to that of commercial Pt/C catalyst.The anion exchange membrane(AEM)electrolyzer,consisting of optimized Mo_(2)N-Ni and NiFe-LDH,achieves a current density of 500 mA cm^(-2)at 1.80 V and can operate stably for 300 h.This assembly method offers an effective strategy for the large-scale preparation of efficient catalysts.
基金supported by the National Natural Science Foundation of China(grant numbers 32170450 and 31872964)Science&Technology Fundamental Resources Investigation Program(grant number 2022FY202100).
文摘Portschinskia Semenov is a rare genus of bot fies whose larvae are obligate parasites of pikas and murine rodents,crucial for understanding the evolutionary biology of Oestridae.However,limited information on their adult biology and early immature stages has hindered the progress.Here,we provided the frst documentation of adult oviposition,behavior,and morphology of newly hatched frst instars of P.magnifca Pleske.Using confocal laser scanning microscopy and scanning electron microscopy,we characterized the ultrastructure of egg and frst-instar larva,identifying key traits facilitating attachment.Eggs were deposited individually or in groups,and glued on non-host surfaces with white adhesive substances,without specialized attachment organ.Newly hatched frst instars were circled with an anterior spinose band on each body segment except the anal division,awaited hosts in an upright position,and anchored to the egg shell by their anal division,likely supported by curved spines originating from the peritreme of the posterior spiracles.Evolutionary analyses of reproductive behavior across the 4 bot fy subfamilies reveal at least 3 times independent evolution of oviposition on non-host surfaces in Portschinskia or the Hypodermatinae clade as a sister group to Ochotonia,Gasterophilus pecorum(Fabricius)(Gasterophilinae),and Cuterebrinae.In contrast,species in the Oestrinae are larviparous,depositing frst instars directly onto hosts.Our fndings shed light on oviposition behavior and early immature morphology of the rare genus Portschinskia,offering insights into reproductive strategies and evolutionary adaptations of bot fies.
基金CAs Youth Interdisciplinary Team(JCTD-2022-06)the National Nature Science Foundation of China(31870209).
文摘Chromosomal rearrangements(CRs)often cause phenotypic variations.Although several major rearrangements have been identified in Triticeae,a comprehensive study of the order,timing,and breakpoints of CRs has not been conducted.Here,we reconstruct high-quality ancestral genomes for the most recent common ancestor(MRCA)of the Triticeae,and the MRCA of the wheat lineage(Triticum and Aegilops).The protogenes of MRCA of the Triticeae and the wheat lineage are 22,894 and 29,060,respectively,which were arranged in their ancestral order.By partitioning modern Triticeae chromosomes into sets of syntenic regions and linking each to the corresponding protochromosomes,we revisit the rye chromosome structural evolution and propose alternative evolutionary routes.The previously identified 4L/5L reciprocal translocation in rye and Triticum urartu is found to have occurred independently and is unlikely to be the result of chromosomal introgression following distant hybridization.We also clarify that the 4AL/7BS translocation in tetraploid wheat was a bidirectional rather than unidirectional translocation event.Lastly,we identify several breakpoints in protochromosomes that independently reoccur following Triticeae evolution,representing potential CR hotspots.This study demonstrates that these reconstructed ancestral genomes can serve as special comparative references and facilitate a better understanding of the evolution of structural rearrangements in Triticeae.
基金supported by the National Key Research and Development Program of China(2021YFC2101303)the National Natural Science Foundation of China(U22A20424 and 22378048)+5 种基金the Major scientific and technological projects of Sinopecthe Dalian Technology Talents Project for Distinguished Young Scholars(2021RJ03)the Fundamental Research Funds for the Central Universities(DUT25LAB104)the Liaoning Revitalization Talents Program(XLYC2202049)the Ningbo Natural Science Foundation(2022J013)the Ningbo Municipal Public Welfare Science and Technology Foundation(2024S004)。
文摘Saccharomyces cerevisiae is not naturally capable of efficiently utilizing xylose as a carbon source.When cultured with lignocellulosic hydrolysates containing pretreatment-derived inhibitors,S.cerevisiae suffers from much lower sugar uptake,ethanol yield and fermentation efficiency.Thus,considering efficient xylose conversion into ethanol during non-detoxified hydrolysate culture,genetic engineering and adaptive evolution of S.cerevisiae might be a promising joint strategy for improving xylose uptake and ethanol production.In this study,an inhibitor-tolerant strain S.cerevisiae SPSC01-TAF94 was genetically engineered by overexpressing both xylose transport-and metabolism-related genes(N360F,Ru-xyl A,TAL1,TKL1,RKI1 and RPE1),yielding the xylose-utilizing strain TAF94-X,followed by three-stage adaptation in non-detoxified corn stover hydrolysate containing 5 g·L^(-1)acetic acid,0.32 g·L^(-1)furfural,0.17 g·L(-1)HMF and 0.19 g·L^(-1)vanillin as the major inhibitors as well as 20,40 and 60 g·L^(-1)xylose adjusted as the major carbon source,respectively.Finally,an active xylose-utilizing and ethanolproducing strain TAF94-X60 was obtained,which achieved 44.9 g·L^(-1)ethanol with yield of0.41 g·g^(-1),productivity of 0.62 g·L^(-1)·h^(-1)and xylose consumption rate of 0.42 g·L^(-1)·h^(-1)during hydrolysate culture,compared to those of 36.5 g·L^(-1),0.38 g·g^(-1),0.50 g·L^(-1)·h^(-1)and 0.20 g·L^(-1)·h^(-1)obtained with the control strain TAF94-X.The proposed joint strategy effectively utilizes hydrolyzed sugars while eliminating the need for conventional detoxification or water washing processes,thus enhancing the economic feasibility of large-scale lignocellulosic ethanol production.
基金supported by the National Key R&D Program of China(2022YFF1001400)postdoctoral innovative talents support program(517000-X92308)+2 种基金the specific research fund of The Innovation Platform for Academicians of Hainan Province(YSPTZX202154,YSPTZX202139)the Research Startup Funding from Hainan Institute of Zhejiang University(0202-6602-A12201)the Distinguished Discipline Support Program of Zhejiang University(226-2024-00205,226-2022-00100).
文摘The Malvaceae family,the most diverse family in the order Malvales,consists of nine subfamilies.Within the Firmiana genus of the Sterculioideae subfamily,most species are considered globally vulnerable,yet their genomes remain unexplored.Here,we present a chromosome-level genome assembly for a representative Firmiana species,F.hainanensis,2n=40,totaling 1536 Mb.Phylogenomic analysis shows that F.hainanensis and Durio zibethinus have the closest evolutionary relationship,with an estimated divergence time of approximately 21 millions of years ago(MYA)and distinct polyploidization events in their histories.Evolutionary trajectory analyses indicate that fissions and fusions may play a crucial role in chromosome number variation(2n=14 to 2n=96).Analysis of repetitive elements among Malvaceae reveals that the Tekay subfamily(belonging to the Gypsy group)contributes to variation in genome size(ranging from 324 Mb to 1620 Mb).Additionally,genes associated with P450,peroxidase,and microtubules,and thereby related to cell wall biosynthesis,are significantly contracted in F.hainanensis,potentially leading to its lower wood density relative to Hopea hainanensis.Overall,our study provides insights into the evolution of chromosome number,genome size,and the genetic basis of cell wall biosynthesis in Malvaceae species.
文摘A composite electrocatalyst,CoMoNiO-S/NF-110(NF is nickel foam),was synthesized through electrodeposition,followed by pyrolysis and then the vulcanization process.CoMoNiO-S/NF-110 exhibited a structure where Ni3S2 and Mo2S3 nanoparticles were integrated at the edges of Co3O4 nanosheets,creating a rich,heterogeneous interface that enhances the synergistic effects of each component.In an alkaline electrolyte,the synthesized CoMoNiO-S/NF-110 exhibited superior electrocatalytic performance for oxygen evolution reaction(OER),achieving current densities of 100 and 200 mA·cm^(-2) with low overpotentials of 199.4 and 224.4 mV,respectively,outperforming RuO2 and several high-performance Mo and Ni-based catalysts.This excellent performance is attributed to the rich interface formed between the components and active sites exposed by the defect structure.
基金supported by the Biological Breeding-Major Projects(2023ZD04076)the Joint Fund of the National Natural Science Foundation of China and the Karst Science Research Center of Guizhou Province(Grant No.U1812401)+1 种基金the National Natural Science Foundation of China(Grant No.31600032)the Start-Up Funding from the Chengdu Institute of Biology,Chinese Academy of Sciences.
文摘The fungal order Botryosphaeriales includes numerous ecologically and economically important plant-associated taxa,yet its genomic diversity and evolutionary mechanisms remain poorly understood.Here,we present high-quality de novo genome assemblies for three representative species—Botryosphaeria dothidea,Neofusicoccum parvum,and Phyllosticta capitalensis—and perform integrative analyses using comparative genomics,population genetics,and pan-genome frameworks.Pathogenic species(B.dothidea and N.parvum)exhibit significant expansions in gene families related to membrane transport and metabolism,suggesting enhanced adaptability and virulence potential.Selective sweep analyses highlight population-level divergence in metabolic and stress-response pathways,reflecting natural selection in host and environmental adaptation.Cross-species pan-genome comparisons of six Phyllosticta species reveal a conserved core genome,dynamic gene family turnover,and extensive horizontal gene transfer from bacterial,and archaeal sources—potentially driving ecological diversification.Furthermore,effector proteins display striking domain variation across genera,particularly in regions associated with host cell wall targeting,indicating convergent strategies for host adaptation.Together,these findings provide comprehensive insights into the genomic evolution,adaptation,and virulence mechanisms of Botryosphaeriales fungi,laying a foundation for future studies on plant–fungal interactions.
文摘The poor electrical conductivity of metal-organic frameworks(MOFs)limits their electrocatalytic performance in the oxygen evolution reaction(OER).In this study,a Py@Co-MOF composite material based on pyrene(Py)molecules and{[Co2(BINDI)(DMA)_(2)]·DMA}_(n)(Co-MOF,H4BINDI=N,N'-bis(5-isophthalic acid)naphthalenediimide,DMA=N,N-dimethylacetamide)was synthesized via a one-pot method,leveragingπ-πinteractions between pyrene and Co-MOF to modulate electrical conductivity.Results demonstrate that the Py@Co-MOF catalyst exhibited significantly enhanced OER performance compared to pure Co-MOF or pyrene-based electrodes,achieving an overpotential of 246 mV at a current density of 10 mA·cm^(-2) along with excellent stability.Density functional theory(DFT)calculations reveal that the formation of O*in the second step is the rate-determining step(RDS)during the OER process on Co-MOF,with an energy barrier of 0.85 eV due to the weak adsorption affinity of the OH*intermediate for Co sites.CCDC:2419276.
