objective To study the clonal evolution of domestic children with lymphoid malignancies inorder to choose the optimal method for the detection of the minimal residual disease. methods To use the PCRwhich employs 22pri...objective To study the clonal evolution of domestic children with lymphoid malignancies inorder to choose the optimal method for the detection of the minimal residual disease. methods To use the PCRwhich employs 22primers and SSCP, investigating the matched samples obtained at diagnosis and at relapse of 13children with lymphoid malignancies. Results The clonal evolution occurred in 54%, 23%, 38%, 46%, 54%, 62%and 54% of 13 cases by PCR employing IgH, TCRγ, TCRVδ2, - Dδ3, TCRPVJ1, TCRPVJ2, TCRPD1,J2, TCRβD2J2primers respectively. NO change occurred only in one of those cases. Clonal evolution at relapse occurred at 50% ofsamples which had only one band PCR product at diagnosis and 78% of those had multiple bands PCR product atdiagnosis. No differences had been detected between diagnosis and relapse by SSCP analyzing those samples inwhich no band change occurred between that at diagnosis and at relapse in PAGE. Conclusion It should beoptimal that the one band rearranged gene at diagnosis acts as main monitoring marker, simultaneously referringto the main band in multiple bands rearranged gene in order to avoid false negative.展开更多
Nonlinear time series prediction is studied by using an improved least squares support vector machine （LSSVM） regression based on chaotic mutation evolutionary programming （CMEP） approach for parameter optimizatio...Nonlinear time series prediction is studied by using an improved least squares support vector machine （LSSVM） regression based on chaotic mutation evolutionary programming （CMEP） approach for parameter optimization. We analyze how the prediction error varies with different parameters （σ, γ） in LS-SVM. In order to select appropriate parameters for the prediction model, we employ CMEP algorithm. Finally, Nasdaq stock data are predicted by using this LS-SVM regression based on CMEP, and satisfactory results are obtained.展开更多
Haematococcus pluvialis(H.pluvialis)is a critical natural source of astaxanthin,recognized for its potent antioxidantcapacity,anti-inflammatory properties,and ability to suppress the proliferation of breast and skin c...Haematococcus pluvialis(H.pluvialis)is a critical natural source of astaxanthin,recognized for its potent antioxidantcapacity,anti-inflammatory properties,and ability to suppress the proliferation of breast and skin cancer cells.The sizedifference between microalgae such as Chlorella vulgaris(C.vulgaris)and Haematococcus pluvialis(H.pluvialis),coupledwith their high research value,makes size-based separation of microalgae essential for efficient extraction of valuablespecies and advancing directed algal evolution.In this study,we introduce an innovative method utilizing microfluidicdevices that integrate spiral channels with contraction-expansion channels.We systematically examine how variablessuch as flow rate,cell concentration,cell size,and three distinct coupling configurations impact cell sorting.Our resultshighlight that each coupling configuration of the spiral and contraction-expansion channels exerts unique controlover sorting performance,offering promising new approaches for optimizing microchannel design.展开更多
This paper, based on the study results of a subproject under the Seventh Five-year-plan National Major Scientific and Technological Research Project, will concentrate on the application of the mixed integer goal progr...This paper, based on the study results of a subproject under the Seventh Five-year-plan National Major Scientific and Technological Research Project, will concentrate on the application of the mixed integer goal programming in the studies on the optimization and comprehensive evaluation of investment decision and production planning in Yichang phosphate rock district in Hubei Province and in Dianchi district in Yunnan Province. The optimization models for mineral district development planning system are formulated through system analysis. A microcomputer software based on the combination of mixed integer programming and goal programming is developed to continuously implement the calculation for the mathematical model with arbitrary objective priority levels and for a group of such models. And a system economic evaluation model is developed to measure the profitability of optimal planning system scenarios. Finally, a large amount of output information are provided for the reference of decision makers.展开更多
The regime of plate tectonics on early Earth is one of the fundamental problems in Earth sciences.Precambrian era takes the majority(ca.88%)of Earth’s history and thus plays a key role in understanding the onset of p...The regime of plate tectonics on early Earth is one of the fundamental problems in Earth sciences.Precambrian era takes the majority(ca.88%)of Earth’s history and thus plays a key role in understanding the onset of plate tectonics and the mechanism,distribution and process of Precambrian subduction zones.This paper presents a review on the progresses of subduction and subduction zones in different stages of Precambrian era,and sorts out some key issues and fields that merits further attention.We suggest that there was progressive onset and evolution of subduction and plate tectonics from Archean to Proterozoic eras.We emphasize the importance of comprehensive studies on subduction mechanism,metamorphic type,plate tectonics regime,the compositional evolution of continental crust,and petrogenesis of diverse granitoids formed in the Archean.It is proposed that innovative analytical techniques,big data,experimental petrology and numerical geodynamic modeling will facilitate future studies of Precambrian subduction zones.展开更多
文摘objective To study the clonal evolution of domestic children with lymphoid malignancies inorder to choose the optimal method for the detection of the minimal residual disease. methods To use the PCRwhich employs 22primers and SSCP, investigating the matched samples obtained at diagnosis and at relapse of 13children with lymphoid malignancies. Results The clonal evolution occurred in 54%, 23%, 38%, 46%, 54%, 62%and 54% of 13 cases by PCR employing IgH, TCRγ, TCRVδ2, - Dδ3, TCRPVJ1, TCRPVJ2, TCRPD1,J2, TCRβD2J2primers respectively. NO change occurred only in one of those cases. Clonal evolution at relapse occurred at 50% ofsamples which had only one band PCR product at diagnosis and 78% of those had multiple bands PCR product atdiagnosis. No differences had been detected between diagnosis and relapse by SSCP analyzing those samples inwhich no band change occurred between that at diagnosis and at relapse in PAGE. Conclusion It should beoptimal that the one band rearranged gene at diagnosis acts as main monitoring marker, simultaneously referringto the main band in multiple bands rearranged gene in order to avoid false negative.
基金The project supported by National Natural Science Foundation of China under Grant No. 90203008 and the Doctoral Foundation of the Ministry of Education of China
文摘Nonlinear time series prediction is studied by using an improved least squares support vector machine （LSSVM） regression based on chaotic mutation evolutionary programming （CMEP） approach for parameter optimization. We analyze how the prediction error varies with different parameters （σ, γ） in LS-SVM. In order to select appropriate parameters for the prediction model, we employ CMEP algorithm. Finally, Nasdaq stock data are predicted by using this LS-SVM regression based on CMEP, and satisfactory results are obtained.
基金supported by National Natural Science Foundation of China(grant numbers 52075138,61964006 and 62364011)Hainan Province Science and Technology Special Fund(grant numbers ZDYF2022SHFZ301).
文摘Haematococcus pluvialis(H.pluvialis)is a critical natural source of astaxanthin,recognized for its potent antioxidantcapacity,anti-inflammatory properties,and ability to suppress the proliferation of breast and skin cancer cells.The sizedifference between microalgae such as Chlorella vulgaris(C.vulgaris)and Haematococcus pluvialis(H.pluvialis),coupledwith their high research value,makes size-based separation of microalgae essential for efficient extraction of valuablespecies and advancing directed algal evolution.In this study,we introduce an innovative method utilizing microfluidicdevices that integrate spiral channels with contraction-expansion channels.We systematically examine how variablessuch as flow rate,cell concentration,cell size,and three distinct coupling configurations impact cell sorting.Our resultshighlight that each coupling configuration of the spiral and contraction-expansion channels exerts unique controlover sorting performance,offering promising new approaches for optimizing microchannel design.
文摘This paper, based on the study results of a subproject under the Seventh Five-year-plan National Major Scientific and Technological Research Project, will concentrate on the application of the mixed integer goal programming in the studies on the optimization and comprehensive evaluation of investment decision and production planning in Yichang phosphate rock district in Hubei Province and in Dianchi district in Yunnan Province. The optimization models for mineral district development planning system are formulated through system analysis. A microcomputer software based on the combination of mixed integer programming and goal programming is developed to continuously implement the calculation for the mathematical model with arbitrary objective priority levels and for a group of such models. And a system economic evaluation model is developed to measure the profitability of optimal planning system scenarios. Finally, a large amount of output information are provided for the reference of decision makers.
基金the National Natural Science Foundation of China(Grant Nos.42025202,41890833&41430210).
文摘The regime of plate tectonics on early Earth is one of the fundamental problems in Earth sciences.Precambrian era takes the majority(ca.88%)of Earth’s history and thus plays a key role in understanding the onset of plate tectonics and the mechanism,distribution and process of Precambrian subduction zones.This paper presents a review on the progresses of subduction and subduction zones in different stages of Precambrian era,and sorts out some key issues and fields that merits further attention.We suggest that there was progressive onset and evolution of subduction and plate tectonics from Archean to Proterozoic eras.We emphasize the importance of comprehensive studies on subduction mechanism,metamorphic type,plate tectonics regime,the compositional evolution of continental crust,and petrogenesis of diverse granitoids formed in the Archean.It is proposed that innovative analytical techniques,big data,experimental petrology and numerical geodynamic modeling will facilitate future studies of Precambrian subduction zones.
