Two new indole alkaloids,evodiagenine 1 and dievodiamine 2 were isolated from the fruits of Evodia rutaecarpa(Juss.) Benth. The structure of compounds 1 and 2 were elucidated by comprehensive spectroscopic analysis an...Two new indole alkaloids,evodiagenine 1 and dievodiamine 2 were isolated from the fruits of Evodia rutaecarpa(Juss.) Benth. The structure of compounds 1 and 2 were elucidated by comprehensive spectroscopic analysis and compound 1 was confirmed by X-ray crystallographic analysis.展开更多
Aim To investigate the chemical constituents of the unripe fruits of Evodia rutaecarpa （Juss.） Benth. and provide a scientific basis for its quality control. Methods The compounds were isolated by silica gel column ...Aim To investigate the chemical constituents of the unripe fruits of Evodia rutaecarpa （Juss.） Benth. and provide a scientific basis for its quality control. Methods The compounds were isolated by silica gel column chromatography and identified by spectral analysis. Results Sixteen compounds were identified as evodiamine （1）, rutaecarpine （2）, β-sitosterol （3）, isorhamnetin（4）, evodol （5）, quercetin （6）, limonin （7）, wuzhuyurutine A （8）, evodirutaenin （9）, shihulimonin A （10）, wuzhuyurutine B（ll）, wuchuyuamide Ⅰ （12）, daucosterol （13）, trans-caffeic acid methylate （14）, dehydroevodiamine （15）and sucrose （16）. Conclusion Compounds 4, 6, 10 and 14 were isolated from the title plant for the first time.展开更多
(±)-Evodiakine(1a and 1b),a pair of rearranged rutaecarpine-type alkaloids with an unprecedented 6/5/5/7/6 ring system,were isolated from the nearly ripe fruits of Evodia rutaecarpa.Separation of the enantiomers ...(±)-Evodiakine(1a and 1b),a pair of rearranged rutaecarpine-type alkaloids with an unprecedented 6/5/5/7/6 ring system,were isolated from the nearly ripe fruits of Evodia rutaecarpa.Separation of the enantiomers have been achieved by chiral HPLC column.The structures of(±)-evodiakine were unambiguously elucidated by 1D and 2D NMR spectra,mass spectrometry,and single-crystal X-ray diffraction.Their absolute configurations were determined by comparison of experimental and calculated electronic circular dichroism spectra.A hypothetical biogenetic pathway for(±)-evodiakine was also proposed.Compounds 1a,1b,and the racemate(1)were tested for their cytotoxic and anti-inflammatory activities.展开更多
Background:Traditional Chinese medicine involves complex ingredients and mixtures of ingredients that often exhibit low bioavailability,and excipients are often lacking to increase the absorption-enhancing effects.Thi...Background:Traditional Chinese medicine involves complex ingredients and mixtures of ingredients that often exhibit low bioavailability,and excipients are often lacking to increase the absorption-enhancing effects.This study modified the generation 4 polyamidoamine dendrimer with polyethylene glycol of different molecular weights(5000,2000,1000)to form a series of polyamidoamine-co-polyethylene glycol(PAMAM-co-PEG)as a novel class of oral absorption enhancers.Evodiamine,the major alkaloid found in the traditional Chinese medicine Wu Zhu Yu(Fructus Evodiae),was used as a model drug to verify the absorption-enhancing effects and the safety of this alkaloid.Methods:This study utilized the solubility determination method documented in the Pharmacopoeia of the People’s Republic of China(2015 edition)and the D0 values recommended in the US FDA guidelines to comprehensively evaluate the solubility of evodiamine.The permeability of evodiamine was assessed using the apparent permeability coefficient in experiments based on in vitro cell models.Multiple aspects of the biological safety of PAMAM-co-PEG were explored using the MTT assay,LDH assay,and total protein release of the rat intestinal tract.Moreover,the absorption-enhancing effects of PAMAM-co-PEG at different molecular weights on evodiamine were verified via the use of in vitro cell models and in vivo intestinal loop circulation experiments with rats.Results:Evodiamine exhibited low solubility and permeability and was classified into class IV compounds using the biopharmaceutical classification system.PAMAM-co-PEG 2000 demonstrated improvement in the biosafety and absorption-enhancement effect of evodiamine at a specific concentration.This study showed that 0.05%(w/v)of PAMAM-co-PEG 2000 increased the cumulative penetration of evodiamine via cell transport by 1.32 times,and 0.10%(w/v)of PAMAM-co-PEG 2000 increased the area under curve value of evodiamine by 1.31 times.Conclusion:Evodiamine possesses low solubility and permeability and leads to poor oral bioavailability and a certain degree of cytotoxicity.PAMAM-co-PEG 2000 was found to be a potentially safe and efficient oral absorption enhancer.The results of this study might create a foundation for the development of novel excipients suitable for the complex active ingredients of traditional Chinese medicine.展开更多
Two new indole alkaloids. wuchuyuamide I and II were isolated from the fruits of Evodia rutaecarpa (Juss.) Benth and their structures were elucidated on the basis of spectral data.
Evodia rutaecarpa (E.R.) is a commonly used Chinese herbal medicine. However, it exerts certain hepatotoxicity and the underlying molecular mechanism has not been clarified. In this study, we investigated the molecula...Evodia rutaecarpa (E.R.) is a commonly used Chinese herbal medicine. However, it exerts certain hepatotoxicity and the underlying molecular mechanism has not been clarified. In this study, we investigated the molecular mechanism involved in hepatotoxicity induced by E.R. Mice were treated with E.R. water- and ethanol-extract at dosage equivalent to 16.67 g crude-drug/kg body weight by intragastric administration once a day on 30 consecutive days. The effect of E.R. extract on liver, manifested by histopathologic effects, liver index, and blood biochemical indexes were tested. In addition, interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor (TNF)-α in liver tissue were measured. The signaling transduction molecules were determined by antibody microarray assay, and verified by western blot. E.R. extract, either water- or ethanol-extract, can induce liver dysfunction. Signaling molecules, Erk1/2, Src, CDK8 and CK1e, were involved in this process. E.R. extract can induce Ck1e expression and phosphorylation of Erk1/2 and CDK8, and inhibit Src phosphorylation. Inflammatory cytokines in liver tissue, IL-1β, IL-6, IL-8, and TNF-α were markedly increased upon the treatment of E.R. extract. In conclusion, E.R.-induced hepatotoxicity was due to the expression of inflammatory cytokine, which was mediated through Erk1/2, Src, CDK8 and CK1e.展开更多
Background:Oral ulcer is the most common and easily recurrent disease in stomatology,which influence the patients’communication,normal dietary,and sleep.Evodia rutaecarpa(ER)is a traditional Chinese herbal medicine r...Background:Oral ulcer is the most common and easily recurrent disease in stomatology,which influence the patients’communication,normal dietary,and sleep.Evodia rutaecarpa(ER)is a traditional Chinese herbal medicine recorded in ancient Chinese medical books,which has a medicinal history of more than 2,000 years.Clinically,the application of ER at plantar Yongquan point(KI1)is effective in the treatment of oral ulcer.The purpose of this study was to combine the modern transdermal drug delivery system with traditional Chinese medicine to develop the transdermal absorption patch of ER and apply it to the treatment of oral ulcer at Yongquan point of plantar.Methods:Firstly,the medicinal materials of ER were extracted and the extracted materials were prepared into dispersed ER patch.The formulation and preparation process were screened by orthogonal design method and single factor investigation method.The adhesive and transdermal properties of the patch were used as the evaluation index of the preparation.Secondly,Wistar rats were used as experimental animals to establish a rat model of mouth ulcers.Wistar rats were randomly divided into normal group,model group(A),model group(B),low dose group,medium dose group and high dose group.the efficacy of ER on rat’s oral ulcer model was evaluated through three aspect such as apparent index,pathological index and biochemical index.Results:The patch had suitable adhesion and good skin penetration,which was an effective treatment for oral ulcer.In vivo pharmacodynamic studies,compared with the normal group,the body mass and food intake of rats in each group after modeling decreased,the amount of drinking water increased,the tissue structure of oral mucosa was damaged,and the levels of inflammatory factors(TNF-α,IL-6)and malondialdehyde increased,the levels of anti-inflammatory factors(IL-10),cell growth factor(epidermal growth factor,TGF-β_(1))and superoxide dismutase decreased.Compared with the model group,the body weight and food intake of each dosing group increased,water consumption decreased,the oral mucosal tissue structure was more complete,the levels of TNF-α,IL-6 and malondialdehyde decreased,the levels of IL-10,epidermal growth factor,TGF-β1 and superoxide dismutase increased,and the changes of various indexes were dose-related.Conclusion:ER patch can inhibit inflammatory reaction,enhance the antioxidant defense ability of the body,and promote the repair of damaged oral mucosa,so as to play an effective role in the treatment of oral ulcer.展开更多
The herb pair is the basic form of compatibility and application of traditional Chinese medicine.Herb pairs have the advantages of simple structure,clear compatibility and effective characteristics,and are suitable fo...The herb pair is the basic form of compatibility and application of traditional Chinese medicine.Herb pairs have the advantages of simple structure,clear compatibility and effective characteristics,and are suitable for scientific research.Rhizoma Coptidis-Fructus Evodiae is a relatively fixed combination of two herbs commonly used in clinical Chinese medicine.Chinese medicine uses it to treat various diseases caused by damp-heat accumulation in the liver and gallbladder,and spleen and stomach.The two medicines in the herb pair are opposite in nature but complement each other,and can achieve a great effect based on simple combination.Modern research shows that the herb pair has a wide range of pharmacological effects and good anti-tumor,analgesic,bacteriostatic and anti-inflammatory activity,and show good curative effects on stress ulcers,functional dyspepsia,chronic colitis,etc.This paper reviewed the research progress on the herb pair of Rhizoma Coptidis-Fructus Evodiae.展开更多
Objective: to analyze the effect of acupoint massage of traditional Chinese medicine nursing combined with acupoint application of fructus evodia on postoperative gastrointestinal function recovery of rectal cancer pa...Objective: to analyze the effect of acupoint massage of traditional Chinese medicine nursing combined with acupoint application of fructus evodia on postoperative gastrointestinal function recovery of rectal cancer patients. Methods: 30 patients with rectal cancer were included in the observation, and the time was set from January 2020 to January 2021. According to nursing differences, the patients were divided into control group and observation group. The former was given routine nursing, while the latter was combined with acupoint massage and acupoint application of fructus evodia. Results: the recovery effect of gastrointestinal function was better than that of control group (P < 0.05). Compared with the data of the control group, the incidence of complications in the observation group was lower, P < 0.05;Compared with the control group, the nursing satisfaction in the observation group was significantly higher (P < 0.05). Conclusion: combined with acupoint massage and evodia rutaecarpa acupoint application, the recovery effect of gastrointestinal function in patients with rectal cancer after operation is better, and can reduce the incidence of complications. At the same time, it has obtained the satisfactory evaluation degree of most patients, which is worthy of promotion.展开更多
Objective To control the quality of Evodia rutaecarpa better.Methods An HPLC-DAD-MS/MS method was established for the rapid and efficient identification of bioactive constituents and for simultaneous quantitative anal...Objective To control the quality of Evodia rutaecarpa better.Methods An HPLC-DAD-MS/MS method was established for the rapid and efficient identification of bioactive constituents and for simultaneous quantitative analysis of four bioactive ingredients including evodiamine,rutaecarpine,dehydroevodiamine,and evodin in E.rutaecarpa,which was applied to evaluating eight samples of E.rutaecarpa and its varieties from different areas.Results Thirteen potentially bioactive constituents including one flavonoid glycoside,one limonin,four indoloquinazoline alkaloids,and seven quinolone alkaloids were identified in all samples and the contents of dehydroevodiamine,evodine,evodiamine,and rutaecarpine varied widely from 0.10% to 0.51%,0.49% to 3.12%,0.07% to 1.56%,and 0.10% to 0.69%,respectively.Conclusion This method is found to be convenient,fast,accurate,and it is facilitated to improve the quality control standard of E.rutaecarpa and related products.展开更多
Objective： To evaluate the changes in the concentrations of five components in Fructus Evodiae used in Chinese medicine, including evodiamine and glycyrrhizic acid, during processing of Fructus Evodiae with Radix Gly...Objective： To evaluate the changes in the concentrations of five components in Fructus Evodiae used in Chinese medicine, including evodiamine and glycyrrhizic acid, during processing of Fructus Evodiae with Radix Glycyrrhizae extract by using high performance liquid chromatography （HPLC） and to provide a scientific basis for different clinical uses of processed and unprocessed Fructus Evodiae. Methods： The concentrations of the Fructus Evodiae components in processed or unprocessed Fructus Evodiae were evaluated by HPLC using a YMC J＇sphere ODS-H80 column （4.6 mm × 250 ram, 5 μm） with acetonitrile-water-tetrehydrofuran-acetic acid （41：59：1：0.2, v/v/v/v） as the mobile phase. The detection wavelength was 225 nm, the column temperature was 35 %, the flow rate was 1.0 mL/min, and the injection volume was 10 μL. The concentrations of the Radix Glycyrrhizae components were determined by HPLC with a KromasiI-C18 column （4.6 mm × 250 mm, 4 μm） and a gradient elution of acetonitrile （A） and 0.05% aqueous phosphoric acid （B） as the mobile phase. The detection wavelength was 237 nm, the column temperature was 35 ℃, the flow rate was 1.0 mlEmin, and the injection volume was 10μL. Results： The calibration curves of evodia lactone, evodiamine, rutaecarpine, liquiritin, and glycyrrhizin showed good linear relationships （r〉0.99）. The recoveries of evodia lactone, evodiamine, rutaecarpine, liquiritin, and glycyrrhizin were 96.59%, 104.18%, 101.91%, 97.75%, and 97.95%, respectively. The concentrations of the components in processed Fructus Evodiae were obviously different to those in unprocessed Fructus Evodiae. Conclusions： The developed method is rapid and accurate. The results provide a reference for processed Fructus Evodiae and the changes that could be expected in its effects compared to unprocessed Fructus Evodiae.展开更多
Objective To develop a suitable method for the large-scale separation of quinolone alkaloids from the fruits of Evodia rutaecarpa by high-speed counter-current chromatography （HSCCC）. Methods Two solvent systems wer...Objective To develop a suitable method for the large-scale separation of quinolone alkaloids from the fruits of Evodia rutaecarpa by high-speed counter-current chromatography （HSCCC）. Methods Two solvent systems were developed for the separation method. The upper phase was used as the stationary phase, and the lower phase was used as the mobile phase at 35 oCwith the flow rate of 2 mL/min and rotation speed of 855 r/min. Results Using the described method, 1-methyl-2-undecyl- 4（1H）-quinoione （1）, evocarpine （2）, 1-methy-2-[（6Z,9Z}]-6,9-pentade-cadienyl-4- （1H）-quinolone （3）, dihydroevocarpine （4）, and the mixture （5） of 1-methyl-2-[（z^- 10-pentadecenyl]-4（1H）-quinolone （Va） and 1 -methyl-2-[（Z^-6-pentadecenyl]- 4（1H）-quinolone （Vb） could be isolated from a petroleum ether extract. They were identified by 1H-NMR, ~3C-NMR, and MS/MS, and the purities were 94.3%, 95.2%, 96.8%, 98.3%, and 96.8%, respectively. Conclusion Five quinolone alkaloids from the fruits of E. rutaecarpa could be systematically isolated and purified using HSCCC. The presented method is simple and efficient with good potentials on the preparation of reference substances, especially on the quality control of Chinese materia medica.展开更多
文摘Two new indole alkaloids,evodiagenine 1 and dievodiamine 2 were isolated from the fruits of Evodia rutaecarpa(Juss.) Benth. The structure of compounds 1 and 2 were elucidated by comprehensive spectroscopic analysis and compound 1 was confirmed by X-ray crystallographic analysis.
基金The National High-Tech 863 Project (No.2002AA22Z343C)Beijing Sciences Foundation (No.Z0004105040311).
文摘Aim To investigate the chemical constituents of the unripe fruits of Evodia rutaecarpa （Juss.） Benth. and provide a scientific basis for its quality control. Methods The compounds were isolated by silica gel column chromatography and identified by spectral analysis. Results Sixteen compounds were identified as evodiamine （1）, rutaecarpine （2）, β-sitosterol （3）, isorhamnetin（4）, evodol （5）, quercetin （6）, limonin （7）, wuzhuyurutine A （8）, evodirutaenin （9）, shihulimonin A （10）, wuzhuyurutine B（ll）, wuchuyuamide Ⅰ （12）, daucosterol （13）, trans-caffeic acid methylate （14）, dehydroevodiamine （15）and sucrose （16）. Conclusion Compounds 4, 6, 10 and 14 were isolated from the title plant for the first time.
基金the NSFC-Joint Foundation of Yunnan Province(No.U1502223)the National Natural Science Foundation of China(No.21402212)+1 种基金the Science and Technology Program of Yunnan province(No.2015FB173)the CAS“Light of West China”Program and Youth Innovation Promotion Association CAS(X.-D.Wu).
文摘(±)-Evodiakine(1a and 1b),a pair of rearranged rutaecarpine-type alkaloids with an unprecedented 6/5/5/7/6 ring system,were isolated from the nearly ripe fruits of Evodia rutaecarpa.Separation of the enantiomers have been achieved by chiral HPLC column.The structures of(±)-evodiakine were unambiguously elucidated by 1D and 2D NMR spectra,mass spectrometry,and single-crystal X-ray diffraction.Their absolute configurations were determined by comparison of experimental and calculated electronic circular dichroism spectra.A hypothetical biogenetic pathway for(±)-evodiakine was also proposed.Compounds 1a,1b,and the racemate(1)were tested for their cytotoxic and anti-inflammatory activities.
基金This research was funded by National Major Scientific and Technological Special Project for“Significant New Drugs Development”(No.2015ZX09501005)Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(No.2016-I2M-1-012).
文摘Background:Traditional Chinese medicine involves complex ingredients and mixtures of ingredients that often exhibit low bioavailability,and excipients are often lacking to increase the absorption-enhancing effects.This study modified the generation 4 polyamidoamine dendrimer with polyethylene glycol of different molecular weights(5000,2000,1000)to form a series of polyamidoamine-co-polyethylene glycol(PAMAM-co-PEG)as a novel class of oral absorption enhancers.Evodiamine,the major alkaloid found in the traditional Chinese medicine Wu Zhu Yu(Fructus Evodiae),was used as a model drug to verify the absorption-enhancing effects and the safety of this alkaloid.Methods:This study utilized the solubility determination method documented in the Pharmacopoeia of the People’s Republic of China(2015 edition)and the D0 values recommended in the US FDA guidelines to comprehensively evaluate the solubility of evodiamine.The permeability of evodiamine was assessed using the apparent permeability coefficient in experiments based on in vitro cell models.Multiple aspects of the biological safety of PAMAM-co-PEG were explored using the MTT assay,LDH assay,and total protein release of the rat intestinal tract.Moreover,the absorption-enhancing effects of PAMAM-co-PEG at different molecular weights on evodiamine were verified via the use of in vitro cell models and in vivo intestinal loop circulation experiments with rats.Results:Evodiamine exhibited low solubility and permeability and was classified into class IV compounds using the biopharmaceutical classification system.PAMAM-co-PEG 2000 demonstrated improvement in the biosafety and absorption-enhancement effect of evodiamine at a specific concentration.This study showed that 0.05%(w/v)of PAMAM-co-PEG 2000 increased the cumulative penetration of evodiamine via cell transport by 1.32 times,and 0.10%(w/v)of PAMAM-co-PEG 2000 increased the area under curve value of evodiamine by 1.31 times.Conclusion:Evodiamine possesses low solubility and permeability and leads to poor oral bioavailability and a certain degree of cytotoxicity.PAMAM-co-PEG 2000 was found to be a potentially safe and efficient oral absorption enhancer.The results of this study might create a foundation for the development of novel excipients suitable for the complex active ingredients of traditional Chinese medicine.
文摘Two new indole alkaloids. wuchuyuamide I and II were isolated from the fruits of Evodia rutaecarpa (Juss.) Benth and their structures were elucidated on the basis of spectral data.
文摘Evodia rutaecarpa (E.R.) is a commonly used Chinese herbal medicine. However, it exerts certain hepatotoxicity and the underlying molecular mechanism has not been clarified. In this study, we investigated the molecular mechanism involved in hepatotoxicity induced by E.R. Mice were treated with E.R. water- and ethanol-extract at dosage equivalent to 16.67 g crude-drug/kg body weight by intragastric administration once a day on 30 consecutive days. The effect of E.R. extract on liver, manifested by histopathologic effects, liver index, and blood biochemical indexes were tested. In addition, interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor (TNF)-α in liver tissue were measured. The signaling transduction molecules were determined by antibody microarray assay, and verified by western blot. E.R. extract, either water- or ethanol-extract, can induce liver dysfunction. Signaling molecules, Erk1/2, Src, CDK8 and CK1e, were involved in this process. E.R. extract can induce Ck1e expression and phosphorylation of Erk1/2 and CDK8, and inhibit Src phosphorylation. Inflammatory cytokines in liver tissue, IL-1β, IL-6, IL-8, and TNF-α were markedly increased upon the treatment of E.R. extract. In conclusion, E.R.-induced hepatotoxicity was due to the expression of inflammatory cytokine, which was mediated through Erk1/2, Src, CDK8 and CK1e.
基金This work was supported by the Scientific Research Project of Liaoning Province Education Department(2020LJC16).
文摘Background:Oral ulcer is the most common and easily recurrent disease in stomatology,which influence the patients’communication,normal dietary,and sleep.Evodia rutaecarpa(ER)is a traditional Chinese herbal medicine recorded in ancient Chinese medical books,which has a medicinal history of more than 2,000 years.Clinically,the application of ER at plantar Yongquan point(KI1)is effective in the treatment of oral ulcer.The purpose of this study was to combine the modern transdermal drug delivery system with traditional Chinese medicine to develop the transdermal absorption patch of ER and apply it to the treatment of oral ulcer at Yongquan point of plantar.Methods:Firstly,the medicinal materials of ER were extracted and the extracted materials were prepared into dispersed ER patch.The formulation and preparation process were screened by orthogonal design method and single factor investigation method.The adhesive and transdermal properties of the patch were used as the evaluation index of the preparation.Secondly,Wistar rats were used as experimental animals to establish a rat model of mouth ulcers.Wistar rats were randomly divided into normal group,model group(A),model group(B),low dose group,medium dose group and high dose group.the efficacy of ER on rat’s oral ulcer model was evaluated through three aspect such as apparent index,pathological index and biochemical index.Results:The patch had suitable adhesion and good skin penetration,which was an effective treatment for oral ulcer.In vivo pharmacodynamic studies,compared with the normal group,the body mass and food intake of rats in each group after modeling decreased,the amount of drinking water increased,the tissue structure of oral mucosa was damaged,and the levels of inflammatory factors(TNF-α,IL-6)and malondialdehyde increased,the levels of anti-inflammatory factors(IL-10),cell growth factor(epidermal growth factor,TGF-β_(1))and superoxide dismutase decreased.Compared with the model group,the body weight and food intake of each dosing group increased,water consumption decreased,the oral mucosal tissue structure was more complete,the levels of TNF-α,IL-6 and malondialdehyde decreased,the levels of IL-10,epidermal growth factor,TGF-β1 and superoxide dismutase increased,and the changes of various indexes were dose-related.Conclusion:ER patch can inhibit inflammatory reaction,enhance the antioxidant defense ability of the body,and promote the repair of damaged oral mucosa,so as to play an effective role in the treatment of oral ulcer.
基金Supported by Collaborative Innovation Center of Zhuang and Yao Ethnic Medicine(GJKY[2013]20)Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine(GKJZ[2014]32)+8 种基金Guangxi Ethnic Medicine Resources and Application Research Center of Engineering(GFGGJH[2020]2605)Guangxi Key Discipline:Zhuang Ethnic Medicine(GZXK-Z-20-64)The Eighth Batch of Special Expert Projects of Guangxi Zhuang Autonomous Region(GRCTZ[2019]13)Guangxi Key Research and Development Project(GK AB21196016)National Natural Science Foundation of China(82060695)Guangxi Science and Technology Base and Talent Project(GK AD21238031)Guangxi Zhuang Autonomous Region Philosophy and Social Science Planning Research Project(20BMZ005)Foreign Expert Project of the Ministry of Science and Technology of the People's Republic of China(GXL20200233001)Guangxi First-class Discipline of Traditional Chinese Medicine:Ethnic Medicine(GJKY[2018]12)。
文摘The herb pair is the basic form of compatibility and application of traditional Chinese medicine.Herb pairs have the advantages of simple structure,clear compatibility and effective characteristics,and are suitable for scientific research.Rhizoma Coptidis-Fructus Evodiae is a relatively fixed combination of two herbs commonly used in clinical Chinese medicine.Chinese medicine uses it to treat various diseases caused by damp-heat accumulation in the liver and gallbladder,and spleen and stomach.The two medicines in the herb pair are opposite in nature but complement each other,and can achieve a great effect based on simple combination.Modern research shows that the herb pair has a wide range of pharmacological effects and good anti-tumor,analgesic,bacteriostatic and anti-inflammatory activity,and show good curative effects on stress ulcers,functional dyspepsia,chronic colitis,etc.This paper reviewed the research progress on the herb pair of Rhizoma Coptidis-Fructus Evodiae.
文摘Objective: to analyze the effect of acupoint massage of traditional Chinese medicine nursing combined with acupoint application of fructus evodia on postoperative gastrointestinal function recovery of rectal cancer patients. Methods: 30 patients with rectal cancer were included in the observation, and the time was set from January 2020 to January 2021. According to nursing differences, the patients were divided into control group and observation group. The former was given routine nursing, while the latter was combined with acupoint massage and acupoint application of fructus evodia. Results: the recovery effect of gastrointestinal function was better than that of control group (P < 0.05). Compared with the data of the control group, the incidence of complications in the observation group was lower, P < 0.05;Compared with the control group, the nursing satisfaction in the observation group was significantly higher (P < 0.05). Conclusion: combined with acupoint massage and evodia rutaecarpa acupoint application, the recovery effect of gastrointestinal function in patients with rectal cancer after operation is better, and can reduce the incidence of complications. At the same time, it has obtained the satisfactory evaluation degree of most patients, which is worthy of promotion.
文摘Objective To control the quality of Evodia rutaecarpa better.Methods An HPLC-DAD-MS/MS method was established for the rapid and efficient identification of bioactive constituents and for simultaneous quantitative analysis of four bioactive ingredients including evodiamine,rutaecarpine,dehydroevodiamine,and evodin in E.rutaecarpa,which was applied to evaluating eight samples of E.rutaecarpa and its varieties from different areas.Results Thirteen potentially bioactive constituents including one flavonoid glycoside,one limonin,four indoloquinazoline alkaloids,and seven quinolone alkaloids were identified in all samples and the contents of dehydroevodiamine,evodine,evodiamine,and rutaecarpine varied widely from 0.10% to 0.51%,0.49% to 3.12%,0.07% to 1.56%,and 0.10% to 0.69%,respectively.Conclusion This method is found to be convenient,fast,accurate,and it is facilitated to improve the quality control standard of E.rutaecarpa and related products.
基金Supported by the Innovation Group Project of China Academy of Chinese Medical Sciences(No.ZZ2006096)
文摘Objective： To evaluate the changes in the concentrations of five components in Fructus Evodiae used in Chinese medicine, including evodiamine and glycyrrhizic acid, during processing of Fructus Evodiae with Radix Glycyrrhizae extract by using high performance liquid chromatography （HPLC） and to provide a scientific basis for different clinical uses of processed and unprocessed Fructus Evodiae. Methods： The concentrations of the Fructus Evodiae components in processed or unprocessed Fructus Evodiae were evaluated by HPLC using a YMC J＇sphere ODS-H80 column （4.6 mm × 250 ram, 5 μm） with acetonitrile-water-tetrehydrofuran-acetic acid （41：59：1：0.2, v/v/v/v） as the mobile phase. The detection wavelength was 225 nm, the column temperature was 35 %, the flow rate was 1.0 mL/min, and the injection volume was 10 μL. The concentrations of the Radix Glycyrrhizae components were determined by HPLC with a KromasiI-C18 column （4.6 mm × 250 mm, 4 μm） and a gradient elution of acetonitrile （A） and 0.05% aqueous phosphoric acid （B） as the mobile phase. The detection wavelength was 237 nm, the column temperature was 35 ℃, the flow rate was 1.0 mlEmin, and the injection volume was 10μL. Results： The calibration curves of evodia lactone, evodiamine, rutaecarpine, liquiritin, and glycyrrhizin showed good linear relationships （r〉0.99）. The recoveries of evodia lactone, evodiamine, rutaecarpine, liquiritin, and glycyrrhizin were 96.59%, 104.18%, 101.91%, 97.75%, and 97.95%, respectively. The concentrations of the components in processed Fructus Evodiae were obviously different to those in unprocessed Fructus Evodiae. Conclusions： The developed method is rapid and accurate. The results provide a reference for processed Fructus Evodiae and the changes that could be expected in its effects compared to unprocessed Fructus Evodiae.
基金National Natural Science Foundation of China(811735648127402881274102)
文摘Objective To develop a suitable method for the large-scale separation of quinolone alkaloids from the fruits of Evodia rutaecarpa by high-speed counter-current chromatography （HSCCC）. Methods Two solvent systems were developed for the separation method. The upper phase was used as the stationary phase, and the lower phase was used as the mobile phase at 35 oCwith the flow rate of 2 mL/min and rotation speed of 855 r/min. Results Using the described method, 1-methyl-2-undecyl- 4（1H）-quinoione （1）, evocarpine （2）, 1-methy-2-[（6Z,9Z}]-6,9-pentade-cadienyl-4- （1H）-quinolone （3）, dihydroevocarpine （4）, and the mixture （5） of 1-methyl-2-[（z^- 10-pentadecenyl]-4（1H）-quinolone （Va） and 1 -methyl-2-[（Z^-6-pentadecenyl]- 4（1H）-quinolone （Vb） could be isolated from a petroleum ether extract. They were identified by 1H-NMR, ~3C-NMR, and MS/MS, and the purities were 94.3%, 95.2%, 96.8%, 98.3%, and 96.8%, respectively. Conclusion Five quinolone alkaloids from the fruits of E. rutaecarpa could be systematically isolated and purified using HSCCC. The presented method is simple and efficient with good potentials on the preparation of reference substances, especially on the quality control of Chinese materia medica.
