[Objective] The aim was to lay a foundation for research of contraceptive rodenticide with LDH-C4 as target protein. [ Method] EST sequence of LDH-C gene from black-lipped pika was cloned by PCR with degenerate primer...[Objective] The aim was to lay a foundation for research of contraceptive rodenticide with LDH-C4 as target protein. [ Method] EST sequence of LDH-C gene from black-lipped pika was cloned by PCR with degenerate primers; then the full length open reading frame （ORF） and 3＇UTR sequence were cloned by RACE technique. [ Result] The full length cDNA was 1 498 bp containing an ORF of 996 bp and a 3＇UTR of 486 bp. The ORF encoded a polypeptide of 332 amino acids. The alignment of LDH-C gene ORF nucleotide sequences from different species showed that the gene was conserved even between large taxons. The phylogenic tree showed that black-lipped pika LDH-C was closer to prima- tes and artiodactyla than to rodents. [Conclusion] cDNA sequence of LDH-C gene from black-lipped pika was cloned successfully.展开更多
This paper, that has been introduced at the annual meeting of the Renaissance Society of America （Washington, March 22, 2012）, is a little part of a wider research about migration and movements of people between Wes...This paper, that has been introduced at the annual meeting of the Renaissance Society of America （Washington, March 22, 2012）, is a little part of a wider research about migration and movements of people between Western and Eastern Europe （and vice versa） that, started one year ago, is still in progress. Despite a common thought that had considered, still in 15th century, Hungarians as unculturished and violent people, the town of Ferrara, ruled by the Estes, had welcomed many of them during the 15-16th centuries. They were, above all, and as the sources testify, literati and students. This paper tries to show and analyze the cultural reasons and the background that have determined Hungarians＇ presence in Ferrara during the Renaissance, with the consciousness that if many sources have been studied, many others must be展开更多
Although the impact brought by COVID-19 pandemic is destructive to the majority of companies all over the world,Estee Lauder has proven itself to be an enduring brand that stands strong despite the pandemic headwinds....Although the impact brought by COVID-19 pandemic is destructive to the majority of companies all over the world,Estee Lauder has proven itself to be an enduring brand that stands strong despite the pandemic headwinds.With its unique advantages over its competitors and efficient management,Estee Lauder is capable of dealing with the pandemic challenges effectively and emerging from the current crisis with an even stronger competitive position.展开更多
基金Supported by State Ethnic Affairs Commission of P.R.C.(08XN04)Applicable and Fundamental Research Funds of SichuanProvince(2008JY0068)Academic Culture and TechnologyLeaders in Sichuan Province Foundation~~
文摘[Objective] The aim was to lay a foundation for research of contraceptive rodenticide with LDH-C4 as target protein. [ Method] EST sequence of LDH-C gene from black-lipped pika was cloned by PCR with degenerate primers; then the full length open reading frame （ORF） and 3＇UTR sequence were cloned by RACE technique. [ Result] The full length cDNA was 1 498 bp containing an ORF of 996 bp and a 3＇UTR of 486 bp. The ORF encoded a polypeptide of 332 amino acids. The alignment of LDH-C gene ORF nucleotide sequences from different species showed that the gene was conserved even between large taxons. The phylogenic tree showed that black-lipped pika LDH-C was closer to prima- tes and artiodactyla than to rodents. [Conclusion] cDNA sequence of LDH-C gene from black-lipped pika was cloned successfully.
文摘This paper, that has been introduced at the annual meeting of the Renaissance Society of America （Washington, March 22, 2012）, is a little part of a wider research about migration and movements of people between Western and Eastern Europe （and vice versa） that, started one year ago, is still in progress. Despite a common thought that had considered, still in 15th century, Hungarians as unculturished and violent people, the town of Ferrara, ruled by the Estes, had welcomed many of them during the 15-16th centuries. They were, above all, and as the sources testify, literati and students. This paper tries to show and analyze the cultural reasons and the background that have determined Hungarians＇ presence in Ferrara during the Renaissance, with the consciousness that if many sources have been studied, many others must be
文摘Although the impact brought by COVID-19 pandemic is destructive to the majority of companies all over the world,Estee Lauder has proven itself to be an enduring brand that stands strong despite the pandemic headwinds.With its unique advantages over its competitors and efficient management,Estee Lauder is capable of dealing with the pandemic challenges effectively and emerging from the current crisis with an even stronger competitive position.
