Dendritic spines are small protrusions along dendrites that contain most of the excitatory synapses in principal neurons,playing a crucial role in neuronal function by creating a compartmentalized environment for sign...Dendritic spines are small protrusions along dendrites that contain most of the excitatory synapses in principal neurons,playing a crucial role in neuronal function by creating a compartmentalized environment for signal transduction.The plasticity of spine morphologies provides a tunable handle to regulate calcium signal dynamics,allowing rapid regulation of protein expression necessary to establish and maintain synapses(Cornejo et al.,2022).If excitatory inputs were to be located primarily on dendritic shafts,dendrites would frequently short-circuit,preventing voltage signals from propagating(Cornejo et al.,2022).It is thus not surprising that the structural plasticity of dendritic spines is closely linked to synaptic plasticity and memory formation(Berry and Nedivi,2017).While comprehensive in vitro studies have been conducted,in vivo studies that directly tackle the mechanism of dendritic transport and translation in regulating spine plasticity spatiotemporally are limited.展开更多
Screening gene function in vivo is a powerful approach to discover novel drug targets. We present high-throughput screening （HTS） data for 3 762 distinct global gene knockout （KO） mouse lines with viable adult hom...Screening gene function in vivo is a powerful approach to discover novel drug targets. We present high-throughput screening （HTS） data for 3 762 distinct global gene knockout （KO） mouse lines with viable adult homozygous mice generated using either gene-trap or homologous recombination technologies. Bone mass was determined from DEXA scans of male and female mice at 14 weeks of age and by microCT analyses of bones from male mice at 16 weeks of age. Wild-type （WT） cagemates/littermates were examined for each gene KO. Lethality was observed in an additional 850 KO lines. Since primary HTS are susceptible to false positive findings, additional cohorts of mice from KO lines with intriguing HTS bone data were examined. Aging, ovariectomy, histomorphometry and bone strength studies were performed and possible non-skeletal phenotypes were explored. Together, these screens identified multiple genes affecting bone mass： 23 previously reported genes （Calcr, Cebpb, Crtap, Dcstamp, Dkkl, Duoxa2, Enppl, Fgf23, Kissl/Kisslr, Kl （Klotho）, Lrp5, Mstn, Neol, Npr2, Ostml, Postn, Sfrp4, S1c30a5, Sic39a13, Sost, Sumf1, Src, Wnt10b）, five novel genes extensively characterized （Cldn18, Fam20c, Lrrkl, Sgpll, Wnt16）, five novel genes with preliminary characterization （Agpat2, RassfS, Slc10a7, Stc26a7, Slc30a10） and three novel undisclosed genes coding for potential osteoporosis drug targets.展开更多
The systemic response to tissue injury, regardless of cause is characterized by a cytokine-mediated alteration in the hepatic synthesis of a number of different plasma proteins,known collectively as 'acute pha... The systemic response to tissue injury, regardless of cause is characterized by a cytokine-mediated alteration in the hepatic synthesis of a number of different plasma proteins,known collectively as 'acute phase reactants'. These proteins include C-reactive protein, serum amyloid A protein, alphal glycoprotein, ceruloplasmin, alpha macroglobulins, complement components (C1-C4, factor B, C9, C11), alpha1antitrypsin, alpha1 antichymotrypsin, fibrinogen, prothrombin,factor Ⅷ, plasminogen, haptoglobin, ferritin, immunoglobulins and lipoproteins. The initiation of the acute phase response is linked to the production of hormone-like polypeptide mediators now called cytokines, namedly, interleukin 1(IL-1),tumor necrosis factor, interferon gamma, interleukin 6 (IL-6),leukemia inhibitory factor, ciliary neurotropic factor, oncostatin M, and interleukin 11 (IL- 11).……展开更多
Effects of transplanted seedling density and species on sward structure of native warm-season grass (NWSG) stands were compared in a randomized complete block design. About 6-week-old NWSG (big bluestem (BB, Andropogo...Effects of transplanted seedling density and species on sward structure of native warm-season grass (NWSG) stands were compared in a randomized complete block design. About 6-week-old NWSG (big bluestem (BB, Andropogon gerardii Vitman), eastern gamagrass (GG, Tripsacum dactyloides L.), indiangrass [IG, Sorghastrum nutans (L.) Nash] and switchgrass (SG, Panicum virgatum) seedlings were transplanted in 45-cm wide rows on clean-tilled seedbeds. Within-row spacing was 30, 25, or, 20 cm giving 10, 12, and 15 plants m-2 as low, medium, and high seedling density, respectively. During establishment, the stands were allowed uninterrupted first year growth without fertilizers or irrigation but when necessary, tall-growing broadleaf weeds were mechanically removed. In the following spring, all dead standing biomass was mowed down to allow emerging tillers access to sunlight. During the second year after planting, early-spring basal diameters, row-length intercepted by the NWSG crowns, mid-summer sward heights, and percentage bare ground were determined. From the second June after planting, and for two consecutive years, plots were harvested twice year-1 to assess forage biomass. Data showed that, unlike species, seedling density had no effect on the assessed parameters. Cumulative forage biomass, in kg DM ha-1, was the least for GG (4901) at low and the most (18,245) for SG at high seedling density during the second year. Corresponding values for the third year were 4500 and 7799 kg DM ha-1. Basal diameters ranged from 18 cm (BB) to 24 cm (IG) while percent row intercepts were from 6 (GG) to 46 (IG) with sward heights measuring 41 cm (IG) to 54 cm (GG). In each stand, percent ground cover by the NWSGs, and at every seedling density, averaged 60.5. Transplanting at ≥10 plant m-2 resulted in harvest-ready stands by the second year of establishment. And while close spacing favored the NWSGs against weeds, data showed that an initial plant density of >10 plants m-2 may not result in increased forage production worthy the additional establishment cost. Data on response to fertility management and forage quality attributes are necessary for more reliable practical recommendations.展开更多
BACKGROUND： Electrical stimulation kindling model, having epilepsy-inducing and spontaneous seizure and other advantages, is a very ideal experimental animal model. But the kindling effect might be different at diffe...BACKGROUND： Electrical stimulation kindling model, having epilepsy-inducing and spontaneous seizure and other advantages, is a very ideal experimental animal model. But the kindling effect might be different at different sites. OBJECTIVE： To compare the features of animal models of complex partial epilepsy established through unilateral, bilateral and alternate-side kindling at hippocampus and successful rate of modeling among these 3 different ways. DESIGN： A randomized and controlled animal experiment SETTING： Department of Neurology, Qilu Hospital, Shandong University MATERIALS： Totally 60 healthy adult Wistar rats, weighing 200 to 300 g, of either gender, were used in this experiment. BL-410 biological functional experimental system （Taimeng Science and Technology Co. Ltd, Chengdu） and SE-7102 type electronic stimulator （Guangdian Company, Japan） were used in the experiment. METHODS： This experiment was carried out in the Experimental Animal Center of Shandong University from April to June 2004. After rats were anesthetized, electrode was implanted into the hippocampus. From the first day of measurement of afterdischarge threshold value, rats were given two-square-wave suprathreshold stimulation once per day with 400 μA intensity, 1ms wave length, 60 Hz frequency for 1 s duration. Left hippocampus was stimulated in unilateral kindling group, bilateral hippocampi were stimulated in bilateral kindling group, and left and right hippocampi were stimulated alternately every day in the alternate-side kindling group. Seizure intensity was scored： grade 0： normal, 1： wet dog-like shivering, facial spasm, such as, winking, touching the beard, rhythmic chewing and so on; 2： rhythmic nodding; 3： forelimb spasm;4： standing accompanied by bilateral forelimb spasm;5： tumbling, losing balance, four limbs spasm. Modeling was successful when seizure intensity reached grade 5. t test was used for the comparison of mean value between two samples. MAIN OUTCOME MEASURES： Comparison of the successful rate of modeling, the times of stimulation to reach intensity of grade 5, the lasting time of seizure of grade 3 of rats in each group. RESULTS： Four rats of alternate-side kindling group dropped out due to infection-induced electrode loss, and 56 rats were involved in the result analysis. The successful rate of unilateral kindling group, bilateral kin- dling group and alternate-side kindling group was 55%（11/20）,100%（16/16）and 100%（20/20）, respective- ly. The stimuli to reach the grade 5 spasm were significantly more in the bilateral kindling group than in the unilateral kindling group [（30.63±3.48）, （19.36±3.47）times, t=8.268, P 〈 0.01], and those were significantly fewer in the alternate-side kindling group than in the unilateral kindling group [（ 10.85±1.98）times, t=-8.744, P 〈 0.01]. The duration of grade 3 spasm was significantly longer in the bilateral kindling group than in the unilateral kindling group [（9.75±2.59）, （3.21 ±1.58）days,t=-8.183,P 〈 0.01], Among 20 successful rats of al- ternate-side kindling group, grade 5 spasm was found in the left hippocampi of 11 rats, but grade 3 spasm in their right hippocampi; Grade 5 spasm was found in the right hippocampi of the other 9 rats, grade 4 spasm in the left hippocampus of 1 rat and grade 3 of 8 rats. CONCLUSION ： The speed of establishing epilepsy seizure model by alternate-side kindling is faster than that by unilateral kindling, while that by bilateral kindling is slower than that by unilateral kindling. The successful rate is very high to establish complex partial epilepsy with alternate-side or bilateral kindling. Epilepsy seizure established by alternate-side kindling has antagonistic effect of kindling and the seizure duration of grade 3 spasm is prolonged.展开更多
Xinjiang Institute of Ecology and Geography（XJEG）,Chinese Academy of Science（CAS）,established three new research departments in 2011.They are Department of Geology Mineralization and Mineral Resources,Department o...Xinjiang Institute of Ecology and Geography（XJEG）,Chinese Academy of Science（CAS）,established three new research departments in 2011.They are Department of Geology Mineralization and Mineral Resources,Department of Environmental Pollution and Process Control, and Department of Central Asian Cooperation and Coordinated Development, respectively. Now, there are a total of eight research departments in XJEG, CAS.展开更多
SAC recently approved the estabLishment of four national technicaL standa「d innovation bases respectively focusing on smart manufacturing, direct current transion and power electronic tech no Logy, rare earth, and gi...SAC recently approved the estabLishment of four national technicaL standa「d innovation bases respectively focusing on smart manufacturing, direct current transion and power electronic tech no Logy, rare earth, and ginseng industry, as a way to impLeme nt the strategy of inno vati on drivi ng deveLopme nt and promote the tran sformati on of tech no logical achieveme nts into tech nical standards.展开更多
The inaugural meeting and first plenary meeting of national technical subcommittee on Al(SAC/TC 28/SC 42)were held on August 6 in both physical and virtual forms.At the inaugural meeting,the approval document of estab...The inaugural meeting and first plenary meeting of national technical subcommittee on Al(SAC/TC 28/SC 42)were held on August 6 in both physical and virtual forms.At the inaugural meeting,the approval document of establishing the subcommittee was announced together with work responsibilities and member List of the subcommittee.And certificates were awarded to chair,vice-chair,secretary and deputy secretary of the subcommittee.展开更多
The establishment of the State Power Grid Development Corp Ltd(SPG) was officially announced on June 18, marking the government’s determination to form a nationwide united power network to make the best of power gene...The establishment of the State Power Grid Development Corp Ltd(SPG) was officially announced on June 18, marking the government’s determination to form a nationwide united power network to make the best of power generating capability. The State-owned corporation, with 2.5 billion yuan ($301 million) worth of operating capital raised by the government, will be responsible for investment. construction and management of the power transmission and substation projects involved in the construction of the Three Gorges Project.展开更多
China's High Performance Computer Standard Commission was established on March 28, 2007, under the guidance of the Science and Technology Bureau of the Ministry of Information Industry. It will prepare relevan... China's High Performance Computer Standard Commission was established on March 28, 2007, under the guidance of the Science and Technology Bureau of the Ministry of Information Industry. It will prepare relevant professional standards on high performance computers to break through the monopoly in the field by foreign manufacturers and vendors.……展开更多
The inaugural meeting of international standards alliance for proton,superconductor and nuclear energy application under the"Belt and Road"Initiative was held in Hefei,Anhui Province on December 4,2017.Repre...The inaugural meeting of international standards alliance for proton,superconductor and nuclear energy application under the"Belt and Road"Initiative was held in Hefei,Anhui Province on December 4,2017.Representatives of China,Russia,Italy,Thailand and Pakistan signed the international standards alliance initiative,in an effort to create a new situation for the application of proton,superconductor and nuclear energy based on collaboration,sharing and win-win.展开更多
Through three-year’s efforts, a nationwide unitary certification and accreditation system has already been established in China, which has legalized and standardized China’s certification and accreditation work. App...Through three-year’s efforts, a nationwide unitary certification and accreditation system has already been established in China, which has legalized and standardized China’s certification and accreditation work. Apporved by the State Council, the Certification and Accreditation Administration of China (CNCA), which is in charge of supervising, administrating and coordinating certification and accreditation departments of the whole nation, has done a lot of work to achieve this goal.展开更多
基金supported by the National Natural Science Foundation of China(NSFC/RGC/JRF N_HKU735/21)Research Grant Council of Hong Kong,China(17102120,17108821,17103922,C1024-22GF,C7074-21G)+1 种基金Health and Medical Research Fund(HMRF 09200966)(to CSWL)FRQS Postdoctoral Fellowship(to AHKF).
文摘Dendritic spines are small protrusions along dendrites that contain most of the excitatory synapses in principal neurons,playing a crucial role in neuronal function by creating a compartmentalized environment for signal transduction.The plasticity of spine morphologies provides a tunable handle to regulate calcium signal dynamics,allowing rapid regulation of protein expression necessary to establish and maintain synapses(Cornejo et al.,2022).If excitatory inputs were to be located primarily on dendritic shafts,dendrites would frequently short-circuit,preventing voltage signals from propagating(Cornejo et al.,2022).It is thus not surprising that the structural plasticity of dendritic spines is closely linked to synaptic plasticity and memory formation(Berry and Nedivi,2017).While comprehensive in vitro studies have been conducted,in vivo studies that directly tackle the mechanism of dendritic transport and translation in regulating spine plasticity spatiotemporally are limited.
文摘Screening gene function in vivo is a powerful approach to discover novel drug targets. We present high-throughput screening （HTS） data for 3 762 distinct global gene knockout （KO） mouse lines with viable adult homozygous mice generated using either gene-trap or homologous recombination technologies. Bone mass was determined from DEXA scans of male and female mice at 14 weeks of age and by microCT analyses of bones from male mice at 16 weeks of age. Wild-type （WT） cagemates/littermates were examined for each gene KO. Lethality was observed in an additional 850 KO lines. Since primary HTS are susceptible to false positive findings, additional cohorts of mice from KO lines with intriguing HTS bone data were examined. Aging, ovariectomy, histomorphometry and bone strength studies were performed and possible non-skeletal phenotypes were explored. Together, these screens identified multiple genes affecting bone mass： 23 previously reported genes （Calcr, Cebpb, Crtap, Dcstamp, Dkkl, Duoxa2, Enppl, Fgf23, Kissl/Kisslr, Kl （Klotho）, Lrp5, Mstn, Neol, Npr2, Ostml, Postn, Sfrp4, S1c30a5, Sic39a13, Sost, Sumf1, Src, Wnt10b）, five novel genes extensively characterized （Cldn18, Fam20c, Lrrkl, Sgpll, Wnt16）, five novel genes with preliminary characterization （Agpat2, RassfS, Slc10a7, Stc26a7, Slc30a10） and three novel undisclosed genes coding for potential osteoporosis drug targets.
文摘 The systemic response to tissue injury, regardless of cause is characterized by a cytokine-mediated alteration in the hepatic synthesis of a number of different plasma proteins,known collectively as 'acute phase reactants'. These proteins include C-reactive protein, serum amyloid A protein, alphal glycoprotein, ceruloplasmin, alpha macroglobulins, complement components (C1-C4, factor B, C9, C11), alpha1antitrypsin, alpha1 antichymotrypsin, fibrinogen, prothrombin,factor Ⅷ, plasminogen, haptoglobin, ferritin, immunoglobulins and lipoproteins. The initiation of the acute phase response is linked to the production of hormone-like polypeptide mediators now called cytokines, namedly, interleukin 1(IL-1),tumor necrosis factor, interferon gamma, interleukin 6 (IL-6),leukemia inhibitory factor, ciliary neurotropic factor, oncostatin M, and interleukin 11 (IL- 11).……
文摘Effects of transplanted seedling density and species on sward structure of native warm-season grass (NWSG) stands were compared in a randomized complete block design. About 6-week-old NWSG (big bluestem (BB, Andropogon gerardii Vitman), eastern gamagrass (GG, Tripsacum dactyloides L.), indiangrass [IG, Sorghastrum nutans (L.) Nash] and switchgrass (SG, Panicum virgatum) seedlings were transplanted in 45-cm wide rows on clean-tilled seedbeds. Within-row spacing was 30, 25, or, 20 cm giving 10, 12, and 15 plants m-2 as low, medium, and high seedling density, respectively. During establishment, the stands were allowed uninterrupted first year growth without fertilizers or irrigation but when necessary, tall-growing broadleaf weeds were mechanically removed. In the following spring, all dead standing biomass was mowed down to allow emerging tillers access to sunlight. During the second year after planting, early-spring basal diameters, row-length intercepted by the NWSG crowns, mid-summer sward heights, and percentage bare ground were determined. From the second June after planting, and for two consecutive years, plots were harvested twice year-1 to assess forage biomass. Data showed that, unlike species, seedling density had no effect on the assessed parameters. Cumulative forage biomass, in kg DM ha-1, was the least for GG (4901) at low and the most (18,245) for SG at high seedling density during the second year. Corresponding values for the third year were 4500 and 7799 kg DM ha-1. Basal diameters ranged from 18 cm (BB) to 24 cm (IG) while percent row intercepts were from 6 (GG) to 46 (IG) with sward heights measuring 41 cm (IG) to 54 cm (GG). In each stand, percent ground cover by the NWSGs, and at every seedling density, averaged 60.5. Transplanting at ≥10 plant m-2 resulted in harvest-ready stands by the second year of establishment. And while close spacing favored the NWSGs against weeds, data showed that an initial plant density of >10 plants m-2 may not result in increased forage production worthy the additional establishment cost. Data on response to fertility management and forage quality attributes are necessary for more reliable practical recommendations.
文摘BACKGROUND： Electrical stimulation kindling model, having epilepsy-inducing and spontaneous seizure and other advantages, is a very ideal experimental animal model. But the kindling effect might be different at different sites. OBJECTIVE： To compare the features of animal models of complex partial epilepsy established through unilateral, bilateral and alternate-side kindling at hippocampus and successful rate of modeling among these 3 different ways. DESIGN： A randomized and controlled animal experiment SETTING： Department of Neurology, Qilu Hospital, Shandong University MATERIALS： Totally 60 healthy adult Wistar rats, weighing 200 to 300 g, of either gender, were used in this experiment. BL-410 biological functional experimental system （Taimeng Science and Technology Co. Ltd, Chengdu） and SE-7102 type electronic stimulator （Guangdian Company, Japan） were used in the experiment. METHODS： This experiment was carried out in the Experimental Animal Center of Shandong University from April to June 2004. After rats were anesthetized, electrode was implanted into the hippocampus. From the first day of measurement of afterdischarge threshold value, rats were given two-square-wave suprathreshold stimulation once per day with 400 μA intensity, 1ms wave length, 60 Hz frequency for 1 s duration. Left hippocampus was stimulated in unilateral kindling group, bilateral hippocampi were stimulated in bilateral kindling group, and left and right hippocampi were stimulated alternately every day in the alternate-side kindling group. Seizure intensity was scored： grade 0： normal, 1： wet dog-like shivering, facial spasm, such as, winking, touching the beard, rhythmic chewing and so on; 2： rhythmic nodding; 3： forelimb spasm;4： standing accompanied by bilateral forelimb spasm;5： tumbling, losing balance, four limbs spasm. Modeling was successful when seizure intensity reached grade 5. t test was used for the comparison of mean value between two samples. MAIN OUTCOME MEASURES： Comparison of the successful rate of modeling, the times of stimulation to reach intensity of grade 5, the lasting time of seizure of grade 3 of rats in each group. RESULTS： Four rats of alternate-side kindling group dropped out due to infection-induced electrode loss, and 56 rats were involved in the result analysis. The successful rate of unilateral kindling group, bilateral kin- dling group and alternate-side kindling group was 55%（11/20）,100%（16/16）and 100%（20/20）, respective- ly. The stimuli to reach the grade 5 spasm were significantly more in the bilateral kindling group than in the unilateral kindling group [（30.63±3.48）, （19.36±3.47）times, t=8.268, P 〈 0.01], and those were significantly fewer in the alternate-side kindling group than in the unilateral kindling group [（ 10.85±1.98）times, t=-8.744, P 〈 0.01]. The duration of grade 3 spasm was significantly longer in the bilateral kindling group than in the unilateral kindling group [（9.75±2.59）, （3.21 ±1.58）days,t=-8.183,P 〈 0.01], Among 20 successful rats of al- ternate-side kindling group, grade 5 spasm was found in the left hippocampi of 11 rats, but grade 3 spasm in their right hippocampi; Grade 5 spasm was found in the right hippocampi of the other 9 rats, grade 4 spasm in the left hippocampus of 1 rat and grade 3 of 8 rats. CONCLUSION ： The speed of establishing epilepsy seizure model by alternate-side kindling is faster than that by unilateral kindling, while that by bilateral kindling is slower than that by unilateral kindling. The successful rate is very high to establish complex partial epilepsy with alternate-side or bilateral kindling. Epilepsy seizure established by alternate-side kindling has antagonistic effect of kindling and the seizure duration of grade 3 spasm is prolonged.
文摘Xinjiang Institute of Ecology and Geography（XJEG）,Chinese Academy of Science（CAS）,established three new research departments in 2011.They are Department of Geology Mineralization and Mineral Resources,Department of Environmental Pollution and Process Control, and Department of Central Asian Cooperation and Coordinated Development, respectively. Now, there are a total of eight research departments in XJEG, CAS.
文摘SAC recently approved the estabLishment of four national technicaL standa「d innovation bases respectively focusing on smart manufacturing, direct current transion and power electronic tech no Logy, rare earth, and ginseng industry, as a way to impLeme nt the strategy of inno vati on drivi ng deveLopme nt and promote the tran sformati on of tech no logical achieveme nts into tech nical standards.
文摘The inaugural meeting and first plenary meeting of national technical subcommittee on Al(SAC/TC 28/SC 42)were held on August 6 in both physical and virtual forms.At the inaugural meeting,the approval document of establishing the subcommittee was announced together with work responsibilities and member List of the subcommittee.And certificates were awarded to chair,vice-chair,secretary and deputy secretary of the subcommittee.
文摘The establishment of the State Power Grid Development Corp Ltd(SPG) was officially announced on June 18, marking the government’s determination to form a nationwide united power network to make the best of power generating capability. The State-owned corporation, with 2.5 billion yuan ($301 million) worth of operating capital raised by the government, will be responsible for investment. construction and management of the power transmission and substation projects involved in the construction of the Three Gorges Project.
文摘 China's High Performance Computer Standard Commission was established on March 28, 2007, under the guidance of the Science and Technology Bureau of the Ministry of Information Industry. It will prepare relevant professional standards on high performance computers to break through the monopoly in the field by foreign manufacturers and vendors.……
文摘The inaugural meeting of international standards alliance for proton,superconductor and nuclear energy application under the"Belt and Road"Initiative was held in Hefei,Anhui Province on December 4,2017.Representatives of China,Russia,Italy,Thailand and Pakistan signed the international standards alliance initiative,in an effort to create a new situation for the application of proton,superconductor and nuclear energy based on collaboration,sharing and win-win.
文摘Through three-year’s efforts, a nationwide unitary certification and accreditation system has already been established in China, which has legalized and standardized China’s certification and accreditation work. Apporved by the State Council, the Certification and Accreditation Administration of China (CNCA), which is in charge of supervising, administrating and coordinating certification and accreditation departments of the whole nation, has done a lot of work to achieve this goal.
