Objective:To evaluate the medicinal uses of Rhanterium epapposum Oliv.(R.epapposum) growing in northern border region of Saudi Arabia,through the chemical diversity of essential oils extracted from its flowers,leaves ...Objective:To evaluate the medicinal uses of Rhanterium epapposum Oliv.(R.epapposum) growing in northern border region of Saudi Arabia,through the chemical diversity of essential oils extracted from its flowers,leaves and stems.Methods:Aerial parts of R.epapposum were collected in April 2014.Air dried flowers,leaves,and stems were separately subjected to hydrodistillation in a Clevenger-type apparatus for 4 h to extract the essential oils.Gas chromatography-mass spectrometry analysis of the essential oils was carried out using an Agilent 6890 gas chromatograph equipped with an Agilent 5973 mass spectrometric detector.Results:A total of 51 compounds representing 76.35%–94.86% of flowers,leaves and stems oils composition were identified.The chemical profiles of the studied fractions revealed the dominance of monoterpenes,regardless of qualitative and quantitative differences observed.Limonene,linalool,4-terpineol and a-cadinol represented the major constituents of flowers oil.Leaves oil was dominated by limonene,sabinene,a-pinene and b-myrcene whereas linalool,ionole,a-cadinol,b-eudesmol,4-terpineol,and aterpineol were the major constituents of stems oil.Conclusions:Essential oils from flowers,leaves and stems of R.epapposum growing in northern border region of Saudi Arabia are considered as a rich source of monoterpenes which have biological activities.展开更多
This study was designed to prepare silver, zinc and gold nanoparticles (NPs), AgNPs, ZnNPs and AuNPs, by biosynthesis technique using methanolic extract of </span><i><span style="font-family:Verdan...This study was designed to prepare silver, zinc and gold nanoparticles (NPs), AgNPs, ZnNPs and AuNPs, by biosynthesis technique using methanolic extract of </span><i><span style="font-family:Verdana;">Rhanterium</span></i><span style="font-family:Verdana;"> <i>epapposum</i></span><span style="font-family:Verdana;"> flowers using AgNO</span><sub><span style="font-family:Verdana;">3</span></sub><span style="font-family:Verdana;">, Zn (CH</span><sub><span style="font-family:Verdana;">3</span></sub><span style="font-family:Verdana;">CO</span><sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;">)</span><sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;"> and HAuCl</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;">·3H</span><sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;">O as starting materials. The physical properties of the formed NPs were characterized by ultraviolet spectroscopy (UV), X-ray diffraction (XRD), transmission electron microscopy and Fourier transformed infrared spectroscopy (FTIR). The results revealed that, AgNPs were homogeneous and spherical in shape, with average diameter 16.3 nm. While, ZnNPs were approximately triangle and hexagonal shaped, with average diameter 23.5 nm. Most of the synthesized AuNPs were spherical in shape with average diameter 17.9 nm. The antifungal activity of different concentrations of the formed AgNPs, ZnNPs and AuNPs was tested against two human </span></span><span style="font-family:Verdana;">pathogens</span><span style="font-family:Verdana;">:</span><span style="font-family:""> </span><i><span style="font-family:Verdana;">Candida albicans</span></i><span style="font-family:""><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">Aspergillus</span></i><span style="font-family:Verdana;"> <i>melleus</i></span><span style="font-family:Verdana;"> and one plant pathogenic fungus</span></span><span style="font-family:Verdana;">:</span><span style="font-family:""> <i><span style="font-family:Verdana;">Phoma</span></i><span style="font-family:Verdana;"> <i>exigua</i></span><span style="font-family:Verdana;">, using agar diffusion assay. The best results recorded by 120 μg/ml AgNPs against the human pathogen</span></span><span style="font-family:Verdana;">:</span><span style="font-family:Verdana;"> <i>C</i></span><i><span style="font-family:Verdana;">.</span></i><span style="font-family:Verdana;"> <i>albicans</i></span><span style="font-family:Verdana;"> where the inhibition zone was 23.5 mm. Additionally, the cytotoxicity of the tested NPs was evaluated against Breast adenocarcinoma (MCF-7)</span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> Hepatocellular carcinoma (HepG-2) and colorectal carcinoma (HCT 116) human cell lines. The most toxic was AuNPs where the IC50 against MCF-7, HepG2 and HCT116 was 55.02, 66.44 and 169.87 μg/mL respectively.展开更多
基金Supported by Deanship of Scientific Research,Northern Border University,Saudi Arabia(Grant No.434/39)
文摘Objective:To evaluate the medicinal uses of Rhanterium epapposum Oliv.(R.epapposum) growing in northern border region of Saudi Arabia,through the chemical diversity of essential oils extracted from its flowers,leaves and stems.Methods:Aerial parts of R.epapposum were collected in April 2014.Air dried flowers,leaves,and stems were separately subjected to hydrodistillation in a Clevenger-type apparatus for 4 h to extract the essential oils.Gas chromatography-mass spectrometry analysis of the essential oils was carried out using an Agilent 6890 gas chromatograph equipped with an Agilent 5973 mass spectrometric detector.Results:A total of 51 compounds representing 76.35%–94.86% of flowers,leaves and stems oils composition were identified.The chemical profiles of the studied fractions revealed the dominance of monoterpenes,regardless of qualitative and quantitative differences observed.Limonene,linalool,4-terpineol and a-cadinol represented the major constituents of flowers oil.Leaves oil was dominated by limonene,sabinene,a-pinene and b-myrcene whereas linalool,ionole,a-cadinol,b-eudesmol,4-terpineol,and aterpineol were the major constituents of stems oil.Conclusions:Essential oils from flowers,leaves and stems of R.epapposum growing in northern border region of Saudi Arabia are considered as a rich source of monoterpenes which have biological activities.
文摘This study was designed to prepare silver, zinc and gold nanoparticles (NPs), AgNPs, ZnNPs and AuNPs, by biosynthesis technique using methanolic extract of </span><i><span style="font-family:Verdana;">Rhanterium</span></i><span style="font-family:Verdana;"> <i>epapposum</i></span><span style="font-family:Verdana;"> flowers using AgNO</span><sub><span style="font-family:Verdana;">3</span></sub><span style="font-family:Verdana;">, Zn (CH</span><sub><span style="font-family:Verdana;">3</span></sub><span style="font-family:Verdana;">CO</span><sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;">)</span><sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;"> and HAuCl</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;">·3H</span><sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;">O as starting materials. The physical properties of the formed NPs were characterized by ultraviolet spectroscopy (UV), X-ray diffraction (XRD), transmission electron microscopy and Fourier transformed infrared spectroscopy (FTIR). The results revealed that, AgNPs were homogeneous and spherical in shape, with average diameter 16.3 nm. While, ZnNPs were approximately triangle and hexagonal shaped, with average diameter 23.5 nm. Most of the synthesized AuNPs were spherical in shape with average diameter 17.9 nm. The antifungal activity of different concentrations of the formed AgNPs, ZnNPs and AuNPs was tested against two human </span></span><span style="font-family:Verdana;">pathogens</span><span style="font-family:Verdana;">:</span><span style="font-family:""> </span><i><span style="font-family:Verdana;">Candida albicans</span></i><span style="font-family:""><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">Aspergillus</span></i><span style="font-family:Verdana;"> <i>melleus</i></span><span style="font-family:Verdana;"> and one plant pathogenic fungus</span></span><span style="font-family:Verdana;">:</span><span style="font-family:""> <i><span style="font-family:Verdana;">Phoma</span></i><span style="font-family:Verdana;"> <i>exigua</i></span><span style="font-family:Verdana;">, using agar diffusion assay. The best results recorded by 120 μg/ml AgNPs against the human pathogen</span></span><span style="font-family:Verdana;">:</span><span style="font-family:Verdana;"> <i>C</i></span><i><span style="font-family:Verdana;">.</span></i><span style="font-family:Verdana;"> <i>albicans</i></span><span style="font-family:Verdana;"> where the inhibition zone was 23.5 mm. Additionally, the cytotoxicity of the tested NPs was evaluated against Breast adenocarcinoma (MCF-7)</span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> Hepatocellular carcinoma (HepG-2) and colorectal carcinoma (HCT 116) human cell lines. The most toxic was AuNPs where the IC50 against MCF-7, HepG2 and HCT116 was 55.02, 66.44 and 169.87 μg/mL respectively.
