Enzyme biosensors,despite their high specificity and sensitivity,are constrained by the inherent fragility of enzymes.Despite being considered ideal for enzyme protection by in-situ covalent organic frameworks(COFs)im...Enzyme biosensors,despite their high specificity and sensitivity,are constrained by the inherent fragility of enzymes.Despite being considered ideal for enzyme protection by in-situ covalent organic frameworks(COFs)immobilization,the harsh conditions of COFs synthesis often lead to enzyme inactivation.Herein,a strategy was developed to prepare robust enzyme@COFs microcapsules(enzyme@MCCOFs)by the interfacial assembly of hydrophobic COFs spheres to form stable Pickering emulsions,followed by COFs regrowth to reinforce the microcapsules.Compared with conventional methods,this approach confines the enzymes in a mild environ-ment,achievig an impressive encapsulation efficiency of 85.9%.Enzymes are protected from inhospitable conditions by the mechanical robustness of microcapsules COFs shell which also features adjustable pores to enhance substrate transport and exhibit size selectivity.The success of strategy was verified by the construction of enzyme@MCCOFs based on various hydrophobic COFs spheres(water contact angle>90◦)with different pore sizes and degrees of functionalization,and they can be pre-modified to meet specific application requirements.The strategy is simple yet effective.COFs employed in this strategy only requires a slight degree of hydropho-bicity,rather than specific functional groups.In addition,a formula was also proposed to predict and control the size of the enzyme@MCCOFs accurately.A cascade catalytic system based on enzyme@MCCOFs has been emulated for the construction of a colorimetric and electrochemical glucose biosensing platform.This study provides new insight into the construction of enzyme@MCCOFs and their biosensing applications.展开更多
Comprehensive Summary Imbalance in the levels of ascorbic acid(AA)can pose a risk to human health.Therefore,it's essential to establish an accurate method for the detection of AA.In this work,a novel N-doped carbo...Comprehensive Summary Imbalance in the levels of ascorbic acid(AA)can pose a risk to human health.Therefore,it's essential to establish an accurate method for the detection of AA.In this work,a novel N-doped carbon composite(MnO_(x)@NC)with dual enzyme-like activities to detect AA was prepared by calcination of Mn-MOF containing H3TTPCA ligand.展开更多
基金supported by the National Natural Science Foundation of China(22264016 and 22464013).
文摘Enzyme biosensors,despite their high specificity and sensitivity,are constrained by the inherent fragility of enzymes.Despite being considered ideal for enzyme protection by in-situ covalent organic frameworks(COFs)immobilization,the harsh conditions of COFs synthesis often lead to enzyme inactivation.Herein,a strategy was developed to prepare robust enzyme@COFs microcapsules(enzyme@MCCOFs)by the interfacial assembly of hydrophobic COFs spheres to form stable Pickering emulsions,followed by COFs regrowth to reinforce the microcapsules.Compared with conventional methods,this approach confines the enzymes in a mild environ-ment,achievig an impressive encapsulation efficiency of 85.9%.Enzymes are protected from inhospitable conditions by the mechanical robustness of microcapsules COFs shell which also features adjustable pores to enhance substrate transport and exhibit size selectivity.The success of strategy was verified by the construction of enzyme@MCCOFs based on various hydrophobic COFs spheres(water contact angle>90◦)with different pore sizes and degrees of functionalization,and they can be pre-modified to meet specific application requirements.The strategy is simple yet effective.COFs employed in this strategy only requires a slight degree of hydropho-bicity,rather than specific functional groups.In addition,a formula was also proposed to predict and control the size of the enzyme@MCCOFs accurately.A cascade catalytic system based on enzyme@MCCOFs has been emulated for the construction of a colorimetric and electrochemical glucose biosensing platform.This study provides new insight into the construction of enzyme@MCCOFs and their biosensing applications.
基金supported by Youth Science and Technology Star Project of Dalian City(No.2022RQ042)Postgraduate Education Reform Project of Liaoning Normal University(No.YJSJG202418)+2 种基金Undergraduate Scientific Research Training Project(No.JSZDBKSXM2023009)State Key Laboratory of Inorganic Synthesis and Preparative Chemistry,College of Chemistry,Jilin University(Grant No.2024-46)Guangxi Collaborative Innovation Centre of Structure and Property for New Energy and Materials,Science Research,Technology Development Project of Guilin(No.20210102-4).
文摘Comprehensive Summary Imbalance in the levels of ascorbic acid(AA)can pose a risk to human health.Therefore,it's essential to establish an accurate method for the detection of AA.In this work,a novel N-doped carbon composite(MnO_(x)@NC)with dual enzyme-like activities to detect AA was prepared by calcination of Mn-MOF containing H3TTPCA ligand.
