Food-derived soybean peptides,beyond their nutritional value,are thought to have benefits in preventing alcoholic liver disease due to their antioxidant activity.However,the enzymatic preparation process suffers from ...Food-derived soybean peptides,beyond their nutritional value,are thought to have benefits in preventing alcoholic liver disease due to their antioxidant activity.However,the enzymatic preparation process suffers from low enzymatic efficiency and a lack of targeted production of protective peptides against alcoholic liver injury.This study used a pH auto-gradient drop enzymatic hydrolysis method,compared with simultaneous enzymatic hydrolysis and sequential stepwise enzymatic hydrolysis,and selected endopeptidase(alcalase,neutrase,or papain)and exopeptidase(flavourzyme)to create six different enzymatic schemes.All groups displayed a pro-tective effect on HepG2 cells exposed to alcohol-induced oxidative damage.The pH auto-gradient drop enzy-matic hydrolysis A↘P↘F group significantly decreased the ALT,AST,and LDH levels in HepG2 cells,promoted Nrf2 protein expression and reduced ROS production.Mass spectrometry analysis revealed a positive association between soybean peptide sequences enriched with His,Arg,and the presence of basic amino acids,Tyr at the C-terminus,with hepatoprotective effects in the A↘P↘F group.The pH auto-gradient drop enzymatic hydrolysis method(A↘P↘F)represents a viable alternative for preparing highly efficacious hepatoprotective active soy-bean peptides.展开更多
In this study,the oil recovery from pumpkin seed with or without the presence of pigment was evaluated during the aqueous enzymatic extraction process(AEEP).Additionally,the interaction of pumpkin seed coat pigment-pr...In this study,the oil recovery from pumpkin seed with or without the presence of pigment was evaluated during the aqueous enzymatic extraction process(AEEP).Additionally,the interaction of pumpkin seed coat pigment-protochlorophyll(Pchl)with interfacial proteins(globulin-O/W,globulin-W,and albumin-W)during the aqueous extraction process was investigated.When Pchl was removed from pumpkin seeds,the yield of free oil was significantly increased by 50.71%after the first centrifugation.And the total oil recovery increased from 68.59%to 89.91%using enzymatic demulsification.Theβ-sheet percentage of globulin-O/W decreased by 5.6%with the presence of Pchl based on the circular dichroism spectra analysis.The particle size of globulin-W and albumin-W significantly increased in contrast to globulin-O/W.In addition,the increase of surface hydrophobicity and surface sulfhydryl group showed a conformational change of three protein extracts.Fluorescence spectra analyses showed that Pchl quenched the intrinsic fluorescence of globulin-W,albumin-W,and globulin-O/W in a static mode.Thermodynamic analyses indicated that the main force between Pchl and proteins was hydrophobic interactions.The hydrolysis efficiency of Papain was reduced during demulsification in AEEP with the presence of Pchl.Therefore,the removal of pumpkin seed coat pigments facilitated the aqueous extraction of seed oil by getting rid of the interaction of Pchl with its interfacial protein.展开更多
The study quantitatively characterizes the process parameters that enable glucose removal via the glucose oxidase/catalase(GO/CAT)enzymatic system in acidic model solutions.Screening across a pH range of 4.0-5.5 and t...The study quantitatively characterizes the process parameters that enable glucose removal via the glucose oxidase/catalase(GO/CAT)enzymatic system in acidic model solutions.Screening across a pH range of 4.0-5.5 and temperatures from 25 to 50℃identified pH 5.5 and 40℃as optimal for GO,with activity at pH 5.0 reduced by only 2.5%.Under these conditions,catalase retained 86.5%of its maximal activity.Dissolved oxygen concentration was found to be a key factor that impacts glucose conversion.Without oxygenation(initial dissolved oxygen concentration of 7.5-8.0 mg/L),6-h reactions achieved 29.0%glucose removal.Additional oxygenation(9.5-11.0 mg/L),using 1000 U GO with varying CAT activities(0,1000,3000,4500 U),yielded glucose reductions of 2.9%,57.0%,75.9%,and 93.4%,respectively,from an initial glucose concentration of 27 g/L.At an oxygen concentration of 12.5-14.0 mg/L with 1000 U GO and 3000 U CAT,complete glucose oxidation was achieved within 4 h.Hydrogen peroxide exhibited concentration-dependent inhibition of GO at pH 5.5/40℃,with relative activities of 82.6%,80%,and 70.4%at 45,60,and 75 mmol/L H2O2,respectively.Thermal stability assessment revealed that GO retained 90.4%of its activity after 6 h,whereas CAT retained 96.9%.Pasteurization(90℃,10 min)fully inactivated GO and caused 84.9%loss of CAT activity.These findings establish practical operational guidelines-GO:CAT≥1:3 and oxygen concentration>9.5 mg/L for efficient glucose depletion under slightly acidic pH conditions and elevated temperature conditions.展开更多
This study screened for the inhibitory peptides from the collagen of barracuda fish skin then determined its inhibitory effect on hyperlipidemia.The results showed that the collagen was type I,mainly containingα1,α2...This study screened for the inhibitory peptides from the collagen of barracuda fish skin then determined its inhibitory effect on hyperlipidemia.The results showed that the collagen was type I,mainly containingα1,α2,andβchains.Scanning electron microscopy revealed the fiber structure,sheet structure,multilayer aggregation structure,which was loose and porous.For an optimal enzymatic digestion process the pH was 10,the dosage of alkaline protease was 3000 U/g,and the temperature 50℃,with a degree of hydrolysis of 26.03%.The peptides of less than 1 KDa were selected by protein ultrafiltration and LC-MS identification revealed 220 peptides.The results indicated that GAPGFPGPR can be the best inhibitory peptide based on its bioactivity,water solubility,toxicity,ADMET and molecular docking technology.In vitro experiment showed that the GAPGFPGPR had significant inhibitory effects on the cholesterol ester transfer protein.The zebrafish experiment further verified that the GAPGFPGPR significantly reduced the total cholesterol,triacylglycerol,low-density lipoprotein cholesterol levels and increased the high-density lipoprotein cholesterol levels in the hyperlipidemia model.Therefore,the study has demonstrated the potented for developing health care products or drugs to improve hyperlipidemia.展开更多
To optimize the enzymatic hydrolysis process of Boletus edulis for preparing a high-antioxidant yak meat preservative,the Box-Behnken response surface method was used,with DPPH⋅scavenging rate as the key evaluation me...To optimize the enzymatic hydrolysis process of Boletus edulis for preparing a high-antioxidant yak meat preservative,the Box-Behnken response surface method was used,with DPPH⋅scavenging rate as the key evaluation metric.DPPH⋅,⋅OH,O^(2)-⋅,total antioxidant capacity,electronic nose,and headspace-solid phase microextractiongas chromatography-mass spectrometry were used to evaluate the antioxidant properties and flavor quality of the five groups of samples.The results showed that the optimum enzymatic hydrolysis conditions were achieved at an enzyme dosage of 1.8%,a pH of 5.7,and a temperature of 48◦C.Under these conditions,DPPH⋅,⋅OH scavenging rate,and total antioxidant capacity were 91.12%,60.63%,and 451.71μmol/mL,respectively.In addition,the contents of volatile organic compounds(VOCs)in compound enzymewere(FU)higher than those in cellulase(CU),pectinase(GU),and papain(PU)but were not significantly different from those in control group(CK).Eight key flavor substances were detected in FU,which were higher than those in CU,GU,PU,and CK.In addition,Mantel test correlation analysis revealed that 1-hepten-3-one significantly correlated with⋅OH scavenging rate(P<0.01).The findings provide a reference for further research on the enzymatic hydrolysate of Boletus edulis as an antioxidant product in the food field.展开更多
The effect of commercial enzyme preparation having transfructosylation activity on reducing sucrose content and fructooligosaccharides synthesis in buffered sucrose solutions was investigated.In the study,sucrose solu...The effect of commercial enzyme preparation having transfructosylation activity on reducing sucrose content and fructooligosaccharides synthesis in buffered sucrose solutions was investigated.In the study,sucrose solutions(pH=4.0-5.5)at different concentrations(20-400 g/L)were treated with the enzyme(12.5-50 UT)and incubated at 25-60℃ for 6 h.Additionally,the inhibitory effect of the glucose presence(concentration in the range of 10-50 g/L)in 25 g/L sucrose solution was assessed.The study revealed that the enzyme preparation effectively reduces the concentration of sucrose in the entire tested temperature and pH range.The enzymatic hydrolysis was promoted at a more acidic pH,and the synthesis of fructooligosaccharides was promoted at a less acidic pH.Commercial enzymes mainly synthesized 1-kestose(GF2)at low reaction times and up to 60℃.Moreover,higher temperatures ensured a more significant decrease in sucrose concentration.The study showed that glucose acts as an inhibitor of fructosyltransferase.The inhibitory effect was enhanced with the increase in the proportion of glucose.展开更多
基金supported by the National Key Research and Devel-opment Program of China(No.2021YFD2100402).
文摘Food-derived soybean peptides,beyond their nutritional value,are thought to have benefits in preventing alcoholic liver disease due to their antioxidant activity.However,the enzymatic preparation process suffers from low enzymatic efficiency and a lack of targeted production of protective peptides against alcoholic liver injury.This study used a pH auto-gradient drop enzymatic hydrolysis method,compared with simultaneous enzymatic hydrolysis and sequential stepwise enzymatic hydrolysis,and selected endopeptidase(alcalase,neutrase,or papain)and exopeptidase(flavourzyme)to create six different enzymatic schemes.All groups displayed a pro-tective effect on HepG2 cells exposed to alcohol-induced oxidative damage.The pH auto-gradient drop enzy-matic hydrolysis A↘P↘F group significantly decreased the ALT,AST,and LDH levels in HepG2 cells,promoted Nrf2 protein expression and reduced ROS production.Mass spectrometry analysis revealed a positive association between soybean peptide sequences enriched with His,Arg,and the presence of basic amino acids,Tyr at the C-terminus,with hepatoprotective effects in the A↘P↘F group.The pH auto-gradient drop enzymatic hydrolysis method(A↘P↘F)represents a viable alternative for preparing highly efficacious hepatoprotective active soy-bean peptides.
基金This research was financially supported by National Key R&D Program Project(2021YFD2100301)Key R&D Program Project of Xinjiang Autonomous Region(2022A02009).
文摘In this study,the oil recovery from pumpkin seed with or without the presence of pigment was evaluated during the aqueous enzymatic extraction process(AEEP).Additionally,the interaction of pumpkin seed coat pigment-protochlorophyll(Pchl)with interfacial proteins(globulin-O/W,globulin-W,and albumin-W)during the aqueous extraction process was investigated.When Pchl was removed from pumpkin seeds,the yield of free oil was significantly increased by 50.71%after the first centrifugation.And the total oil recovery increased from 68.59%to 89.91%using enzymatic demulsification.Theβ-sheet percentage of globulin-O/W decreased by 5.6%with the presence of Pchl based on the circular dichroism spectra analysis.The particle size of globulin-W and albumin-W significantly increased in contrast to globulin-O/W.In addition,the increase of surface hydrophobicity and surface sulfhydryl group showed a conformational change of three protein extracts.Fluorescence spectra analyses showed that Pchl quenched the intrinsic fluorescence of globulin-W,albumin-W,and globulin-O/W in a static mode.Thermodynamic analyses indicated that the main force between Pchl and proteins was hydrophobic interactions.The hydrolysis efficiency of Papain was reduced during demulsification in AEEP with the presence of Pchl.Therefore,the removal of pumpkin seed coat pigments facilitated the aqueous extraction of seed oil by getting rid of the interaction of Pchl with its interfacial protein.
基金financed by the statutory activity of the Prof.Wacław Dąbrowski Institute of Agricultural and Food Biotechnology-State Research Institute,project No.ZO-126-01.
文摘The study quantitatively characterizes the process parameters that enable glucose removal via the glucose oxidase/catalase(GO/CAT)enzymatic system in acidic model solutions.Screening across a pH range of 4.0-5.5 and temperatures from 25 to 50℃identified pH 5.5 and 40℃as optimal for GO,with activity at pH 5.0 reduced by only 2.5%.Under these conditions,catalase retained 86.5%of its maximal activity.Dissolved oxygen concentration was found to be a key factor that impacts glucose conversion.Without oxygenation(initial dissolved oxygen concentration of 7.5-8.0 mg/L),6-h reactions achieved 29.0%glucose removal.Additional oxygenation(9.5-11.0 mg/L),using 1000 U GO with varying CAT activities(0,1000,3000,4500 U),yielded glucose reductions of 2.9%,57.0%,75.9%,and 93.4%,respectively,from an initial glucose concentration of 27 g/L.At an oxygen concentration of 12.5-14.0 mg/L with 1000 U GO and 3000 U CAT,complete glucose oxidation was achieved within 4 h.Hydrogen peroxide exhibited concentration-dependent inhibition of GO at pH 5.5/40℃,with relative activities of 82.6%,80%,and 70.4%at 45,60,and 75 mmol/L H2O2,respectively.Thermal stability assessment revealed that GO retained 90.4%of its activity after 6 h,whereas CAT retained 96.9%.Pasteurization(90℃,10 min)fully inactivated GO and caused 84.9%loss of CAT activity.These findings establish practical operational guidelines-GO:CAT≥1:3 and oxygen concentration>9.5 mg/L for efficient glucose depletion under slightly acidic pH conditions and elevated temperature conditions.
基金supported by the Open Fund of Institute of Ocean Research,Bohai University(BDHYYJY2024003).
文摘This study screened for the inhibitory peptides from the collagen of barracuda fish skin then determined its inhibitory effect on hyperlipidemia.The results showed that the collagen was type I,mainly containingα1,α2,andβchains.Scanning electron microscopy revealed the fiber structure,sheet structure,multilayer aggregation structure,which was loose and porous.For an optimal enzymatic digestion process the pH was 10,the dosage of alkaline protease was 3000 U/g,and the temperature 50℃,with a degree of hydrolysis of 26.03%.The peptides of less than 1 KDa were selected by protein ultrafiltration and LC-MS identification revealed 220 peptides.The results indicated that GAPGFPGPR can be the best inhibitory peptide based on its bioactivity,water solubility,toxicity,ADMET and molecular docking technology.In vitro experiment showed that the GAPGFPGPR had significant inhibitory effects on the cholesterol ester transfer protein.The zebrafish experiment further verified that the GAPGFPGPR significantly reduced the total cholesterol,triacylglycerol,low-density lipoprotein cholesterol levels and increased the high-density lipoprotein cholesterol levels in the hyperlipidemia model.Therefore,the study has demonstrated the potented for developing health care products or drugs to improve hyperlipidemia.
基金Supported by Sichuan Science and Technology Program(2024YFHZ0295,2025HJRC0041).
文摘To optimize the enzymatic hydrolysis process of Boletus edulis for preparing a high-antioxidant yak meat preservative,the Box-Behnken response surface method was used,with DPPH⋅scavenging rate as the key evaluation metric.DPPH⋅,⋅OH,O^(2)-⋅,total antioxidant capacity,electronic nose,and headspace-solid phase microextractiongas chromatography-mass spectrometry were used to evaluate the antioxidant properties and flavor quality of the five groups of samples.The results showed that the optimum enzymatic hydrolysis conditions were achieved at an enzyme dosage of 1.8%,a pH of 5.7,and a temperature of 48◦C.Under these conditions,DPPH⋅,⋅OH scavenging rate,and total antioxidant capacity were 91.12%,60.63%,and 451.71μmol/mL,respectively.In addition,the contents of volatile organic compounds(VOCs)in compound enzymewere(FU)higher than those in cellulase(CU),pectinase(GU),and papain(PU)but were not significantly different from those in control group(CK).Eight key flavor substances were detected in FU,which were higher than those in CU,GU,PU,and CK.In addition,Mantel test correlation analysis revealed that 1-hepten-3-one significantly correlated with⋅OH scavenging rate(P<0.01).The findings provide a reference for further research on the enzymatic hydrolysate of Boletus edulis as an antioxidant product in the food field.
基金financed by the statutory activity from prof.Waclaw Dabrowski Institute of Agricultural and Food Biotechnology-State Research Institute,project No.ZO-126-01.
文摘The effect of commercial enzyme preparation having transfructosylation activity on reducing sucrose content and fructooligosaccharides synthesis in buffered sucrose solutions was investigated.In the study,sucrose solutions(pH=4.0-5.5)at different concentrations(20-400 g/L)were treated with the enzyme(12.5-50 UT)and incubated at 25-60℃ for 6 h.Additionally,the inhibitory effect of the glucose presence(concentration in the range of 10-50 g/L)in 25 g/L sucrose solution was assessed.The study revealed that the enzyme preparation effectively reduces the concentration of sucrose in the entire tested temperature and pH range.The enzymatic hydrolysis was promoted at a more acidic pH,and the synthesis of fructooligosaccharides was promoted at a less acidic pH.Commercial enzymes mainly synthesized 1-kestose(GF2)at low reaction times and up to 60℃.Moreover,higher temperatures ensured a more significant decrease in sucrose concentration.The study showed that glucose acts as an inhibitor of fructosyltransferase.The inhibitory effect was enhanced with the increase in the proportion of glucose.
