[ Objective] To study the effect of pretreatment with chemical substances on enzymatic saccharification of affalfa （ Medic, ago sativa L. ）, sorghum hybrid sudan grass [ Sorghum bicolor （ L. ） Moench x Sorghum sud...[ Objective] To study the effect of pretreatment with chemical substances on enzymatic saccharification of affalfa （ Medic, ago sativa L. ）, sorghum hybrid sudan grass [ Sorghum bicolor （ L. ） Moench x Sorghum sudanese （Piper） Stapf], erect milkvetch （Astraga/us adsurgens Pall. ） and pearl millet （ Pennisetum americanum （ L. ） Leeke）. [ Method ] The forages were pretreated with sulfuric acid at different concentration, and then the content of cellulose, hemicellulose, and lignin were detected and compared with that before pretreatment. The concentration of glucose and ethanol after different fermentation time was also determined. [ Result] After pretreatment, the content of cellulose increased, while that of hemicel- lulose and lignin decreased. After treatment with 1.0% （W/V） sulfuric acid, the four kinds of forages all had the highest concentration of ethanol in the citric acid-sodium citrate buffer system （pH 4.8）. Dudng fermentation process, the concentration of glucose and ethanol first increased and then declined, peaking respectively at 24 h and 48 h post fermentation. [Condusion] Pretreatment promotes the enzymatic saccharification and fermen- tation of alfalfa, sorehum hvbrid sudan orass. Dead millet, and erect milkvetch, and their enerov performance decreases in order.展开更多
Many effective pretreatment methods (such as dilute acid, dilute alkali, ionic liquids, etc.) have been developed for lignocellulose upgrading, but several defaults of low working mass, high sugar loss and extra cost ...Many effective pretreatment methods (such as dilute acid, dilute alkali, ionic liquids, etc.) have been developed for lignocellulose upgrading, but several defaults of low working mass, high sugar loss and extra cost of solid-liquid separation and water washing hinder their large-scale applica- tion in industry. Besides, the valorization of lignin-rich residue from pretreated biomass after hy- drolysis or fermentation greatly contributes to the economy and sustainability of lignocellulosic biorefinery, which is usually underestimated. This study developed a densification pretreatment with binary chemicals (densifying lignocellulosic biomass with sulfuric acid (SA) and metal salt (MS) followed by autoclave treatment ((DLCA(SA-MS)), which was conducted under mild con- dition (121 ℃) with a biomass working mass as high as 400 kg/m^(3) . The DLCA(SA-MS) biomass achieved over 95% sugar retention, 90% enzymatic sugar conversion and a high concentration of fermentable sugar (212.3 g/L) with superior fermentability. Furthermore, bio-adsorbent de- rived from DLCA(SA-MS) biomass residue was highly adsorptive and suitable for dyeing wastew- ater treatment, providing a feasible and eco-friendly method for lignin-rich residue valorization. These findings indicated that DLCA(SA-MS) pretreatment enables the full-component utilization of biomass and boosts the economic viability of lignocellulosic biorefinery.展开更多
文摘[ Objective] To study the effect of pretreatment with chemical substances on enzymatic saccharification of affalfa （ Medic, ago sativa L. ）, sorghum hybrid sudan grass [ Sorghum bicolor （ L. ） Moench x Sorghum sudanese （Piper） Stapf], erect milkvetch （Astraga/us adsurgens Pall. ） and pearl millet （ Pennisetum americanum （ L. ） Leeke）. [ Method ] The forages were pretreated with sulfuric acid at different concentration, and then the content of cellulose, hemicellulose, and lignin were detected and compared with that before pretreatment. The concentration of glucose and ethanol after different fermentation time was also determined. [ Result] After pretreatment, the content of cellulose increased, while that of hemicel- lulose and lignin decreased. After treatment with 1.0% （W/V） sulfuric acid, the four kinds of forages all had the highest concentration of ethanol in the citric acid-sodium citrate buffer system （pH 4.8）. Dudng fermentation process, the concentration of glucose and ethanol first increased and then declined, peaking respectively at 24 h and 48 h post fermentation. [Condusion] Pretreatment promotes the enzymatic saccharification and fermen- tation of alfalfa, sorehum hvbrid sudan orass. Dead millet, and erect milkvetch, and their enerov performance decreases in order.
基金supported by the National Key R&D Program of China(No.2021YFC2101301).
文摘Many effective pretreatment methods (such as dilute acid, dilute alkali, ionic liquids, etc.) have been developed for lignocellulose upgrading, but several defaults of low working mass, high sugar loss and extra cost of solid-liquid separation and water washing hinder their large-scale applica- tion in industry. Besides, the valorization of lignin-rich residue from pretreated biomass after hy- drolysis or fermentation greatly contributes to the economy and sustainability of lignocellulosic biorefinery, which is usually underestimated. This study developed a densification pretreatment with binary chemicals (densifying lignocellulosic biomass with sulfuric acid (SA) and metal salt (MS) followed by autoclave treatment ((DLCA(SA-MS)), which was conducted under mild con- dition (121 ℃) with a biomass working mass as high as 400 kg/m^(3) . The DLCA(SA-MS) biomass achieved over 95% sugar retention, 90% enzymatic sugar conversion and a high concentration of fermentable sugar (212.3 g/L) with superior fermentability. Furthermore, bio-adsorbent de- rived from DLCA(SA-MS) biomass residue was highly adsorptive and suitable for dyeing wastew- ater treatment, providing a feasible and eco-friendly method for lignin-rich residue valorization. These findings indicated that DLCA(SA-MS) pretreatment enables the full-component utilization of biomass and boosts the economic viability of lignocellulosic biorefinery.
