Bocapavovirus,a member of the genus Bocaparvovirus within the subfamily Parvovirinae and the family Parvoviridae,is a small,non-enveloped,single-stranded DNA virus.This pathogen poses health risks to both humans and a...Bocapavovirus,a member of the genus Bocaparvovirus within the subfamily Parvovirinae and the family Parvoviridae,is a small,non-enveloped,single-stranded DNA virus.This pathogen poses health risks to both humans and animals.The Bocaparvovirus genome.展开更多
Monkeypox was declared a global health emergency by the World Health Organization,and as of March 2023,86,000 confirmed cases and 111 deaths across 110 countries have been reported.Its causal agent,monkeypox virus(MPV...Monkeypox was declared a global health emergency by the World Health Organization,and as of March 2023,86,000 confirmed cases and 111 deaths across 110 countries have been reported.Its causal agent,monkeypox virus(MPV)belongs to a large family of double-stranded DNA viruses,Orthopoxviridae,that also includes vaccinia virus(VACV)and others.MPV produces two distinct forms of viral particles during its replication cycles:the enveloped viron(EV)that is released via exocytosis,and the mature viron(MV)that is discharged through lysis of host cells.This study was designed to develop multi-valent m RNA vaccines against monkeypox EV and MV surface proteins,and examine their efficacy and mechanism of action.Four m RNA vaccines were produced with different combinations of surface proteins from EV(A35R and B6R),MV(A29L,E8L,H3L and M1R),or EV and MV,and were administered in Balb/c mice to assess their immunogenicity potentials.A dynamic immune response was observed as soon as seven days after initial immunization,while a strong Ig G response to all immunogens was detected with ELISA after two vaccinations.The higher number of immunogens contributed to a more robust total Ig G response and correlating neutralizing activity against VACV,indicating the additive potential of each immunogen in generating immune response and nullifying VACV infection.Further,the m RNA vaccines elicited an antigen-specific CD4^(+)T cell response that is biased towards Th1.The m RNA vaccines with different combinations of EVand MV surface antigens protected a mouse model from a lethal dose VACV challenge,with the EV and MV antigens-combined vaccine offering the strongest protection.These findings provide insight into the protective mechanism of multi-valent m RNAvaccines against MPV,and also the foundation for further development of effective and safe m RNA vaccines for enhanced protection against monkeypox virus outbreak.展开更多
Spiral bevel gears are critical transmission components,and are widely used in the aerospace field.This paper proposes a new multi-DOF envelope forming process for fabricating spiral bevel gears.Firstly,the multi-DOF ...Spiral bevel gears are critical transmission components,and are widely used in the aerospace field.This paper proposes a new multi-DOF envelope forming process for fabricating spiral bevel gears.Firstly,the multi-DOF envelope forming principle of spiral bevel gears is proposed.Secondly,the design methods for the envelope tool geometry and movement are proposed based on the envelope geometry and movement relationships.Thirdly,the metal flow and tooth filling laws are revealed through 3D FE simulation of the multi-DOF envelope forming process of a typical spiral bevel gear.Fourthly,a new method for separating the envelope tool and the formed spiral bevel gear with back taper tooth is proposed to avoid their interference.Finally,experiments on multi-DOF envelope forming of this typical spiral bevel gear are conducted using new heavy load multi-DOF envelope forming equipment.The simulation and experimental results show the feasibility of the proposed multi-DOF envelope forming process for fabricating spiral bevel gears with back taper tooth and the corresponding process design methods.展开更多
Ports are crucial to the economy of many nations;thus, numerous studies have been conducted on port efficiency and productivity. This study analyses the efficiency and productivity of some major global ports namely, P...Ports are crucial to the economy of many nations;thus, numerous studies have been conducted on port efficiency and productivity. This study analyses the efficiency and productivity of some major global ports namely, Port of Singapore, Rotterdam, Antwerp and Durban. The main objectives of this study are to determine the level of operational efficiency of the mentioned ports, measure and evaluate the ports’ productivity changes and lastly to investigate the factors influencing the productivity changes of the ports studied. To achieve these objectives, Data Envelopment Analysis (DEA-BCC) model was used to determine the technical and operational efficiencies of the ports and Malmquist productivity index was employed to calculate the various productivity levels. The results of the study can guide stakeholders to formulate their operational strategies for port efficiency and productivity. The study also has policy suggestions that are uniquely targeted to Africa’s issues and potential.展开更多
Studies on coral aggregate concrete(CAC)mainly focus on uniaxial stress conditions.However,concrete structures often experience complex stress conditions in practical engineering.It is essential to investigate the mec...Studies on coral aggregate concrete(CAC)mainly focus on uniaxial stress conditions.However,concrete structures often experience complex stress conditions in practical engineering.It is essential to investigate the mechanical behavior and failure mechanisms of CAC under multiaxial stress conditions.This paper employs a 3D mesoscale model that considers the actual size,shape,and spatial distribution of aggregates.The reliability of the model and material parameters is verified through comparison with existing experimental data.Subsequently,the model is used to systematically study the mechanical properties,failure modes,and failure processes of C40 CAC under the biaxial compression.The numerical results are compared with the experimental results of CAC and ordinary portland concrete(OPC).The results indicate that the failure modes of CAC under the biaxial compression are diagonal shear failure.The biaxial compressive strength and elastic modulus of CAC are greater than those under uniaxial stress and exhibit a significant intermediate principal stress effect.The biaxial compressive strength reaches its maximum value when the stress ratio is 0.5,which is consistent with the conclusions for OPC.Finally,failure criteria and strength envelopes for CAC under the biaxial compression are established in order to provide a reference for analyzing the strength characteristics and structural design of CAC.展开更多
This paper presents a new criterion for determining the unloading points quantitatively and consistently in a multi-stage triaxial test.The radial strain gradient(RSG)is first introduced as an arc tangent function of ...This paper presents a new criterion for determining the unloading points quantitatively and consistently in a multi-stage triaxial test.The radial strain gradient(RSG)is first introduced as an arc tangent function of the rate of change of radial strain to time.RSG is observed to correlate closely with the stress state of a compressed sample,and reaches a horizontal asymptote as approaching failure.For a given rock type,RSG value at peak stress is almost the same,irrespective of the porosity and permeability.These findings lead to the development of RSG criterion:Unloading points can be precisely determined at the time when RSG reaches a pre-determined value that is a little smaller than or equal to the RSG at peak stress.The RSG criterion is validated against other criteria and the single-stage triaxial test on various types of rocks.Failure envelopes from the RSG criterion match well with those from single-stage tests.A practical procedure is recommended to use the RSG criterion:an unconfined compression or single-stage test is first conducted to determine the RSG at peak stress for one sample,the unloading point is then selected to be a value close to the RSG at peak stress,and the multi-stage test is finally performed on another sample using the pre-selected RSG unloading criterion.Generally,the RSG criterion is applicable for any type of rocks,especially brittle rocks,where other criteria are not suitable.Further,it can be practically implemented on the most available rock mechanical testing instruments.展开更多
UAV shipboard landing poses significant challenges in terms of safety and efficiency,due to the oscillatory ship motion caused by wave interactions and wind gusts,especially in rough sea states.To solve this issue,a f...UAV shipboard landing poses significant challenges in terms of safety and efficiency,due to the oscillatory ship motion caused by wave interactions and wind gusts,especially in rough sea states.To solve this issue,a flight envelope constrained fixed-time control strategy is proposed to achieve a reliable UAV landing on a maneuvering ship.Firstly,a sliding data window autoregressive model is designed to predict the ship's roll and pitch motions,which are accordingly utilized to identify an appropriate quiescent period for safe landing.Subsequently,a barrier-function-based nonsingular terminal sliding mode controller is developed to eliminate the tracking errors within the identified quiescent period,while ensuring the errors remain bounded to satisfy flight envelope constraints.In particular,lumped disturbance components are estimated by integrating a fixed-time disturbance observer and compensated in the controller.The key advantage of the proposed approach is that it well balances the control requirements between precise landing position and safe landing attitude,guaranteeing both steady-state performance and transient behavior of the tracking error.Finally,comparative Gazebo simulations in different sea state scenarios are conducted to verify the satisfactory control performance.展开更多
The stratospheric airship is affected by harsh conditions in the stratosphere environment.To ensure the safety of the airship,it is necessary to detect the material state of the airship envelope.Since digital image co...The stratospheric airship is affected by harsh conditions in the stratosphere environment.To ensure the safety of the airship,it is necessary to detect the material state of the airship envelope.Since digital image correlation possesses non-contact strain measurement ability,this paper explores the infuence of different shapes of the subset on measurement accuracy.Through the results,it is found that increasing the aspect ratio of subsets can improve the strain accuracy measured in the c-direction,and reducing the aspect ratio can improve the strain accuracy measured in the y-direction.This trend becomes more obvious as the strain increases.Based on this discovery,a subset adaptive algorithm is proposed.The feasibility of the algorithm is verified by experiments,and the precision of strain measurement can be effectively improved by adjusting the threshold value.Therefore,the algorithm can be utilized to increase the measurement accuracy in the larger strain direction without changing the size of the subset.展开更多
The circumstellar envelopes(CSE) of asymptotic giant branch(AGB) stars are abundant in molecular emissions, offering valuable insights into the physical and chemical conditions of these evolving stars. In this paper, ...The circumstellar envelopes(CSE) of asymptotic giant branch(AGB) stars are abundant in molecular emissions, offering valuable insights into the physical and chemical conditions of these evolving stars. In this paper, we report observations of two molecules(CO and SO_(2)) toward four O-rich AGB stars using the James Clerk Maxwell Telescope(JCMT). We detected an unusual SO_(2) spectral feature comprising both broad and narrow components in IK Tau and AP Lyn. The broad line profiles may originate from thermal molecular emission, while the narrow profiles could come from other species(or masers) or astrophysical phenomena occurring within the CSEs of the AGB stars, such as episodic mass loss, bipolar outflows, or emissions associated with the complex physical processes near the central star. The narrow lines of SO_(2) may also arise from vibrationally excited emissions. Additionally, we observed the same U-line in both TX Cam and IK Tau, which may originate from the molecule N^(17)O. We analyzed the identified molecular lines using rotational diagrams to determine their excitation temperatures, column densities, and fractional abundances. This information aids in the constructing of reliable astrochemical models for a more detailed examination of the target stars. The narrow component of the SO_(2) line suggests unusual astrophysical phenomena, making IK Tau and AP Lyn particularly intriguing for further investigation to fully understand the physical processes at play in these sources.展开更多
The correct evaluation of enterprise technological innovation performance is the premise and foundation to improve the efficiency of enterprise innovation.This paper first puts forward the index system of enterprise t...The correct evaluation of enterprise technological innovation performance is the premise and foundation to improve the efficiency of enterprise innovation.This paper first puts forward the index system of enterprise technological innovation performance evaluation,then establishes the model of technological innovation performance evaluation,explores the quantitative evaluation of enterprise innovation performance by using the DEA method,and finally makes an empirical study on the technological innovation performance of the TR enterprise.展开更多
Enveloped viruses have been the leading causative agents of viral epidemics in the past decade,including the ongoing coronavirus disease 2019 outbreak.In epidemics caused by enveloped viruses,direct contact is a commo...Enveloped viruses have been the leading causative agents of viral epidemics in the past decade,including the ongoing coronavirus disease 2019 outbreak.In epidemics caused by enveloped viruses,direct contact is a common route of infection,while indirect transmissions through the environment also contribute to the spread of the disease,although their significance remains controversial.Bridging the knowledge gap regarding the influence of interfacial interactions on the persistence of enveloped viruses in the environment reveals the transmission mechanisms when the virus undergoes mutations and prevents excessive disinfection during viral epidemics.Herein,from the perspective of the driving force,partition efficiency,and viral survivability at interfaces,we summarize the viral and environmental characteristics that affect the environmental transmission of viruses.We expect to provide insights for virus detection,environmental surveillance,and disinfection to limit the spread of severe acute respiratory syndrome coronavirus 2.展开更多
Enveloped RNA viruses are a group of viruses with an outer membrane derived from a host cell and a genome consisting of ribonucleic acid(RNA).These viruses rely on host cell machinery and organelles to replicate and a...Enveloped RNA viruses are a group of viruses with an outer membrane derived from a host cell and a genome consisting of ribonucleic acid(RNA).These viruses rely on host cell machinery and organelles to replicate and assemble new virus particles.However,the interaction between viruses and host organelles may be disrupted by nanomaterials,such as gold nanoparticles(AuNPs)with unique physical and chemical properties.In this study,we investigated the effects of AuNPs with different surface charge properties on the subcellular structure and function of mammalian cells,and their effects on two representative enveloped RNA viruses:lentivirus and human coronavirus OC43(HCoV-OC43)antiviral potential.By comparing the subcellular effects of AuNPs with different surface charge properties,we found that treatment with AuNPs with positive surface charges induced more significant disruption of subcellular structures than neutrally charged AuNPs and negatively charged AuNPs,mainly manifested in lysosomes and Cytoskeletal disorders.The antiviral effect of the surface positively charged AuNPs was further evaluated using lentivirus and HCoV-OC43.The results showed that AuNPs had a significant inhibitory effect on both lentivirus and HCoV-OC43 without obvious side effects.In conclusion,our study provides insights into the mechanism of action and biocompatibility of AuNP in biological systems,while supporting the potential of targeting organelle dynamics against enveloped RNA viruses.展开更多
Leakages from subsea oil and gas equipment cause substantial economic losses and damage to marine ecosystem,so it is essential to locate the source of the leak.However,due to the complexity and variability of the mari...Leakages from subsea oil and gas equipment cause substantial economic losses and damage to marine ecosystem,so it is essential to locate the source of the leak.However,due to the complexity and variability of the marine environment,the signals collected by hydrophone contain a variety of noises,which makes it challenging to extract useful signals for localization.To solve this problem,a hydrophone denoising algorithm is proposed based on variational modal decomposition(VMD)with grey wolf optimization.First,the average envelope entropy is used as the fitness function of the grey wolf optimizer to find the optimal solution for the parameters K andα.Afterward,the VMD algorithm decomposes the original signal parameters to obtain the intrinsic mode functions(IMFs).Subsequently,the number of interrelationships between each IMF and the original signal was calculated,the threshold value was set,and the noise signal was removed to calculate the time difference using the valid signal obtained by reconstruction.Finally,the arrival time difference is used to locate the origin of the leak.The localization accuracy of the method in finding leaks is investigated experimentally by constructing a simulated leak test rig,and the effectiveness and feasibility of the method are verified.展开更多
Substantially glazed facades are extensively used in contemporary high-rise buildings to achieve attractive architectural aesthetics.Inherent conflicts exist among architectural aesthetics,building energy consumption,...Substantially glazed facades are extensively used in contemporary high-rise buildings to achieve attractive architectural aesthetics.Inherent conflicts exist among architectural aesthetics,building energy consumption,and solar energy harvesting for glazed facades.In this study,we addressed these conflicts by introducing a new dynamic and vertical photovoltaic integrated building envelope(dvPVBE)that offers extraordinary flexibility with weather-responsive slat angles and blind positions,superior architectural aesthetics,and notable energy-saving potential.Three hierarchical control strategies were proposed for different scenarios of the dvPVBE:power generation priority(PGP),natural daylight priority(NDP),and energy-saving priority(ESP).Moreover,the PGP and ESP strategies were further analyzed in the simulation of a dvPVBE.An office room integrated with a dvPVBE was modeled using EnergyPlus.The influence of the dvPVBE in improving the building energy efficiency and corresponding optimal slat angles was investigated under the PGP and ESP control strategies.The results indicate that the application of dvPVBEs in Beijing can provide up to 131%of the annual energy demand of office rooms and significantly increase the annual net energy output by at least 226%compared with static photovoltaic(PV)blinds.The concept of this novel dvPVBE offers a viable approach by which the thermal load,daylight penetration,and energy generation can be effectively regulated.展开更多
Computing tasks may often be posed as optimization problems.The objective functions for real-world scenarios are often nonconvex and/or nondifferentiable.State-of-the-art methods for solving these problems typically o...Computing tasks may often be posed as optimization problems.The objective functions for real-world scenarios are often nonconvex and/or nondifferentiable.State-of-the-art methods for solving these problems typically only guarantee convergence to local minima.This work presents Hamilton-Jacobi-based Moreau adaptive descent(HJ-MAD),a zero-order algorithm with guaranteed convergence to global minima,assuming continuity of the objective function.The core idea is to compute gradients of the Moreau envelope of the objective(which is"piece-wise convex")with adaptive smoothing parameters.Gradients of the Moreau envelope(i.e.,proximal operators)are approximated via the Hopf-Lax formula for the viscous Hamilton-Jacobi equation.Our numerical examples illustrate global convergence.展开更多
The optical potential ambiguity is a long-standing problem in the analysis of elastic scattering data.For a specific collid-ing system,ambiguous potential families can lead to different behaviors in the nearside and f...The optical potential ambiguity is a long-standing problem in the analysis of elastic scattering data.For a specific collid-ing system,ambiguous potential families can lead to different behaviors in the nearside and farside scattering components.By contrast,the envelope method can decompose the experimental data into two components with negative and positive deflection angles,respectively.Hence,a question arises as to whether the comparison between the calculated nearside(or farside)component and the derived positive-deflection-angle(or negative-deflection-angle)component can help analyze the potential ambiguity problem.In this study,we conducted a trial application of the envelope method to the potential ambiguity problem.The envelope method was improved by including uncertainties in the experimental data.The colliding systems of 16O+28Si at 215.2 MeV and 12C+12C at 1016 MeV were considered in the analyses.For each colliding system,the angular distribution experimental data were described nearly equally well by two potential sets,one of which is“surface transpar-ent”and the other is refractive.The calculated angular distributions were decomposed into nearside and farside scattering components.Using the improved envelope method,the experimental data were decomposed into the positive-deflection-angle and negative-deflection-angle components,which were then compared with the calculated nearside and farside components.The capability of the envelope method to analyze the potential ambiguities was also discussed.展开更多
To design the optimum acceleration control schedule for the Adaptive Cycle Engine(ACE)in the full flight envelope,this paper establishes a direct simulation model of the ACE transient state.In this model,geometric par...To design the optimum acceleration control schedule for the Adaptive Cycle Engine(ACE)in the full flight envelope,this paper establishes a direct simulation model of the ACE transient state.In this model,geometric parameters are used to replace the component state parameters.The corresponding relationship between geometric parameters and component state parameters is determined by sensitivity analysis.The geometric variables are controlled when the geometric adjustment speed exceeds the limit,and at the same time the corresponding component state parameters are iterated.The gradient optimization algorism is used to optimize the ground acceleration process of ACE,and the control schedule in terms of operating point of compression components and corrected acceleration rate is used as the full-envelope acceleration control schedule based on the similarity principle.The acceleration control schedules of the triple-bypass mode and the double-bypass mode are designed in this paper.The acceleration processes under various flight conditions are simulated using the acceleration control schedules.Compared with the acceleration process with the linear geometric adjustment schedule,the acceleration performance of ACE is improved by the acceleration control schedule,with the impulse of the acceleration process of the triple-bypass mode being increased by 8.7%-12.3% and the impulse of the double-bypass mode acceleration process being increased by 11.8%-14.1%.展开更多
The development of machine learning technology enables more robust real-time earthquake monitoring through automated implementations. However, the application of machine learning to earthquake location problems faces ...The development of machine learning technology enables more robust real-time earthquake monitoring through automated implementations. However, the application of machine learning to earthquake location problems faces challenges in regions with limited available training data. To address the issues of sparse event distribution and inaccurate ground truth in historical seismic datasets, we expand the training dataset by using a large number of synthetic envelopes that closely resemble real data and build an earthquake location model named ENVloc. We propose an envelope-based machine learning workflow for simultaneously determining earthquake location and origin time. The method eliminates the need for phase picking and avoids the accumulation of location errors resulting from inaccurate picking results. In practical application, ENVloc is applied to several data intercepted at different starting points. We take the starting point of the time window corresponding to the highest prediction probability value as the origin time and save the predicted result as the earthquake location. We apply ENVloc to observed data acquired in the southern Sichuan Basin, China, between September 2018 and March 2019. The results show that the average difference with the catalog in latitude, longitude, depth, and origin time is 0.02°,0.02°, 2 km, and 1.25 s, respectively. These suggest that our envelope-based method provides an efficient and robust way to locate earthquakes without phase picking, and can be used in earthquake monitoring in near-real time.展开更多
The tourism industry is economically very important.According to the World Travel Tourism Council,in 2019,the tourism industry accounted for a quarter of all new jobs created worldwide,10.3%of all jobs,and 9.6×10...The tourism industry is economically very important.According to the World Travel Tourism Council,in 2019,the tourism industry accounted for a quarter of all new jobs created worldwide,10.3%of all jobs,and 9.6×1012 USD of the global gross domestic product.This study aimed to calculate the tourism efficiency index for different Latin American countries from 2010 to 2021 using data envelopment analysis,which analyzes the relationships between input variables(including the number of employees in the tourism industry and the number of hotel-type establishments)and output variables(including tourism expenditures in other countries and public social expenditures in recreation and culture per capita).Additionally,this study aimed to identify the countries with greater tourism development and the factors that may affect the development of the tourism industry through the stochastic frontier production function.The results of the tourism efficiency index for Central America(including Costa Rica,Dominica,El Salvador,Honduras,Mexico,and Panama)and South America(including Argentina,Brazil,Chile,Colombia,Ecuador,Paraguay,Peru,and Uruguay)exhibited different trends.However,after the global health crisis,the tourism industry recovered,showing new opportunities to promote sustainability.The results of the stochastic frontier production function demonstrated that countries with higher levels of inbound and outbound tourism,contribution of tourism to the economy,natural resources,and literacy rate exhibited more efficient tourism industry,whereas countries with higher pollution levels exhibited less efficient tourism industry.The findings of this study could allow us to formulate suitable public policies to promote tourism,maintain natural resources,and diversify these sectors with more inclusive programmes that can facilitate growth and benefit vulnerable communities.展开更多
BACKGROUND Occult hepatitis B infection(OBI)is characterized by the detection of hepatitis B virus(HBV)DNA in serum(usually HBV DNA<200 IU/mL)or the liver but negativity for hepatitis B surface antigen(HBsAg).The d...BACKGROUND Occult hepatitis B infection(OBI)is characterized by the detection of hepatitis B virus(HBV)DNA in serum(usually HBV DNA<200 IU/mL)or the liver but negativity for hepatitis B surface antigen(HBsAg).The diagnosis of OBI relies on the sensitivity of assays used in the detection of HBV DNA and HBsAg.HBsAg assays with inadequate sensitivity or inability to detect HBV S variants may lead to misdiagnosis of OBI in people with overt HBV infection.CASE SUMMARY We report a HBsAg-negative but hepatitis B envelope antigen-positive patient who had a significant HBV DNA level.The patient was initially diagnosed as having OBI.However,sequence analysis revealed a unique insertion of amino acid residues at positions 120-124 in the S protein,which affects the formation of a disulfide bond that is associated with the formation of a loop.It is well known that there is an overlap between the S protein and Pol protein.We found that this new insertion site occurred in polymerase/reverse transcriptase domain,indi-cating that this insertion might be involved in HBV pathogenicity.The patient was finally diagnosed with a false OBI.CONCLUSION An insertion of amino acid residues at positions 120-124 of the S protein affects the formation of immunodominant epitopes and results in negative HBsAg levels.展开更多
基金supported by the Natural Science Foundation of Sichuan Province,China(2024NSFSC1272)the Innovation Team Development Funds for Sichuan Mutton Goat&Sheep,China(SCCXTD-2024-14)Scientific and Technological Innovation Team for Qinghai-Tibetan Plateau Research in Southwest Minzu University,China(2024CXTD08)。
文摘Bocapavovirus,a member of the genus Bocaparvovirus within the subfamily Parvovirinae and the family Parvoviridae,is a small,non-enveloped,single-stranded DNA virus.This pathogen poses health risks to both humans and animals.The Bocaparvovirus genome.
基金the National Science and Technology Major Projects(2021YFC2300704)the National Key Research and Development Program of China(2021YFA1301402,2018YFA0903700)+3 种基金the Strategic Priority Research Program of Chinese Academy of Sciences(XDA24010400)Shanghai Municipal Science and Technology Major Project(ZD2021CY001)the National Natural Science Foundation of China(32270695,31972881)support from Lingang Laboratory(Shanghai,China)。
文摘Monkeypox was declared a global health emergency by the World Health Organization,and as of March 2023,86,000 confirmed cases and 111 deaths across 110 countries have been reported.Its causal agent,monkeypox virus(MPV)belongs to a large family of double-stranded DNA viruses,Orthopoxviridae,that also includes vaccinia virus(VACV)and others.MPV produces two distinct forms of viral particles during its replication cycles:the enveloped viron(EV)that is released via exocytosis,and the mature viron(MV)that is discharged through lysis of host cells.This study was designed to develop multi-valent m RNA vaccines against monkeypox EV and MV surface proteins,and examine their efficacy and mechanism of action.Four m RNA vaccines were produced with different combinations of surface proteins from EV(A35R and B6R),MV(A29L,E8L,H3L and M1R),or EV and MV,and were administered in Balb/c mice to assess their immunogenicity potentials.A dynamic immune response was observed as soon as seven days after initial immunization,while a strong Ig G response to all immunogens was detected with ELISA after two vaccinations.The higher number of immunogens contributed to a more robust total Ig G response and correlating neutralizing activity against VACV,indicating the additive potential of each immunogen in generating immune response and nullifying VACV infection.Further,the m RNA vaccines elicited an antigen-specific CD4^(+)T cell response that is biased towards Th1.The m RNA vaccines with different combinations of EVand MV surface antigens protected a mouse model from a lethal dose VACV challenge,with the EV and MV antigens-combined vaccine offering the strongest protection.These findings provide insight into the protective mechanism of multi-valent m RNAvaccines against MPV,and also the foundation for further development of effective and safe m RNA vaccines for enhanced protection against monkeypox virus outbreak.
基金the National Science and Technology Major Project of China(No.2019-VII0017e0158)the National Natural Science Foundation of China(No.U21A20131)+1 种基金the Industry-University Research Cooperation Project,China(No.HFZL2020CXY025)the National Key Laboratory of Science and Technology on Helicopter Transmission,China(No.HTL-O-21G05).
文摘Spiral bevel gears are critical transmission components,and are widely used in the aerospace field.This paper proposes a new multi-DOF envelope forming process for fabricating spiral bevel gears.Firstly,the multi-DOF envelope forming principle of spiral bevel gears is proposed.Secondly,the design methods for the envelope tool geometry and movement are proposed based on the envelope geometry and movement relationships.Thirdly,the metal flow and tooth filling laws are revealed through 3D FE simulation of the multi-DOF envelope forming process of a typical spiral bevel gear.Fourthly,a new method for separating the envelope tool and the formed spiral bevel gear with back taper tooth is proposed to avoid their interference.Finally,experiments on multi-DOF envelope forming of this typical spiral bevel gear are conducted using new heavy load multi-DOF envelope forming equipment.The simulation and experimental results show the feasibility of the proposed multi-DOF envelope forming process for fabricating spiral bevel gears with back taper tooth and the corresponding process design methods.
文摘Ports are crucial to the economy of many nations;thus, numerous studies have been conducted on port efficiency and productivity. This study analyses the efficiency and productivity of some major global ports namely, Port of Singapore, Rotterdam, Antwerp and Durban. The main objectives of this study are to determine the level of operational efficiency of the mentioned ports, measure and evaluate the ports’ productivity changes and lastly to investigate the factors influencing the productivity changes of the ports studied. To achieve these objectives, Data Envelopment Analysis (DEA-BCC) model was used to determine the technical and operational efficiencies of the ports and Malmquist productivity index was employed to calculate the various productivity levels. The results of the study can guide stakeholders to formulate their operational strategies for port efficiency and productivity. The study also has policy suggestions that are uniquely targeted to Africa’s issues and potential.
基金supported by the National Science Foundations of China(Nos.52078250,51878350,11832013,51678304,51508272)。
文摘Studies on coral aggregate concrete(CAC)mainly focus on uniaxial stress conditions.However,concrete structures often experience complex stress conditions in practical engineering.It is essential to investigate the mechanical behavior and failure mechanisms of CAC under multiaxial stress conditions.This paper employs a 3D mesoscale model that considers the actual size,shape,and spatial distribution of aggregates.The reliability of the model and material parameters is verified through comparison with existing experimental data.Subsequently,the model is used to systematically study the mechanical properties,failure modes,and failure processes of C40 CAC under the biaxial compression.The numerical results are compared with the experimental results of CAC and ordinary portland concrete(OPC).The results indicate that the failure modes of CAC under the biaxial compression are diagonal shear failure.The biaxial compressive strength and elastic modulus of CAC are greater than those under uniaxial stress and exhibit a significant intermediate principal stress effect.The biaxial compressive strength reaches its maximum value when the stress ratio is 0.5,which is consistent with the conclusions for OPC.Finally,failure criteria and strength envelopes for CAC under the biaxial compression are established in order to provide a reference for analyzing the strength characteristics and structural design of CAC.
文摘This paper presents a new criterion for determining the unloading points quantitatively and consistently in a multi-stage triaxial test.The radial strain gradient(RSG)is first introduced as an arc tangent function of the rate of change of radial strain to time.RSG is observed to correlate closely with the stress state of a compressed sample,and reaches a horizontal asymptote as approaching failure.For a given rock type,RSG value at peak stress is almost the same,irrespective of the porosity and permeability.These findings lead to the development of RSG criterion:Unloading points can be precisely determined at the time when RSG reaches a pre-determined value that is a little smaller than or equal to the RSG at peak stress.The RSG criterion is validated against other criteria and the single-stage triaxial test on various types of rocks.Failure envelopes from the RSG criterion match well with those from single-stage tests.A practical procedure is recommended to use the RSG criterion:an unconfined compression or single-stage test is first conducted to determine the RSG at peak stress for one sample,the unloading point is then selected to be a value close to the RSG at peak stress,and the multi-stage test is finally performed on another sample using the pre-selected RSG unloading criterion.Generally,the RSG criterion is applicable for any type of rocks,especially brittle rocks,where other criteria are not suitable.Further,it can be practically implemented on the most available rock mechanical testing instruments.
文摘UAV shipboard landing poses significant challenges in terms of safety and efficiency,due to the oscillatory ship motion caused by wave interactions and wind gusts,especially in rough sea states.To solve this issue,a flight envelope constrained fixed-time control strategy is proposed to achieve a reliable UAV landing on a maneuvering ship.Firstly,a sliding data window autoregressive model is designed to predict the ship's roll and pitch motions,which are accordingly utilized to identify an appropriate quiescent period for safe landing.Subsequently,a barrier-function-based nonsingular terminal sliding mode controller is developed to eliminate the tracking errors within the identified quiescent period,while ensuring the errors remain bounded to satisfy flight envelope constraints.In particular,lumped disturbance components are estimated by integrating a fixed-time disturbance observer and compensated in the controller.The key advantage of the proposed approach is that it well balances the control requirements between precise landing position and safe landing attitude,guaranteeing both steady-state performance and transient behavior of the tracking error.Finally,comparative Gazebo simulations in different sea state scenarios are conducted to verify the satisfactory control performance.
文摘The stratospheric airship is affected by harsh conditions in the stratosphere environment.To ensure the safety of the airship,it is necessary to detect the material state of the airship envelope.Since digital image correlation possesses non-contact strain measurement ability,this paper explores the infuence of different shapes of the subset on measurement accuracy.Through the results,it is found that increasing the aspect ratio of subsets can improve the strain accuracy measured in the c-direction,and reducing the aspect ratio can improve the strain accuracy measured in the y-direction.This trend becomes more obvious as the strain increases.Based on this discovery,a subset adaptive algorithm is proposed.The feasibility of the algorithm is verified by experiments,and the precision of strain measurement can be effectively improved by adjusting the threshold value.Therefore,the algorithm can be utilized to increase the measurement accuracy in the larger strain direction without changing the size of the subset.
基金support from the Natural Science Foundation of Xinjiang Uygur Autonomous Region, China (Grant No. 2024D01E37)the National Natural Science Foundation of China (Grant No. 12473025)+5 种基金supported by grant number ST/T000198/1 from the STFCthe sponsored by Natural Science Foundation of Xinjiang Uygur Autonomous Region, China (Grant No. 2022D01B221)the Xinjiang Tianchi Talent Project (2019)The James Clerk Maxwell Telescope is operated by the East Asian Observatory on behalf of the National Astronomical Observatory of Japan, Academia Sinica Institute of Astronomy and Astrophysics, the Korea Astronomy and Space Science Institute, the National Astronomical Research Institute of Thailand, Center for Astronomical Mega-Science as well as the National Key Research and Development Program of China (Grant No. 2017YFA0402700)support is provided by the Science and Technology Facilities Council of the United Kingdom and participating universities and organizations in the United Kingdom and Canadasupported by the JCMT M22BP013 project。
文摘The circumstellar envelopes(CSE) of asymptotic giant branch(AGB) stars are abundant in molecular emissions, offering valuable insights into the physical and chemical conditions of these evolving stars. In this paper, we report observations of two molecules(CO and SO_(2)) toward four O-rich AGB stars using the James Clerk Maxwell Telescope(JCMT). We detected an unusual SO_(2) spectral feature comprising both broad and narrow components in IK Tau and AP Lyn. The broad line profiles may originate from thermal molecular emission, while the narrow profiles could come from other species(or masers) or astrophysical phenomena occurring within the CSEs of the AGB stars, such as episodic mass loss, bipolar outflows, or emissions associated with the complex physical processes near the central star. The narrow lines of SO_(2) may also arise from vibrationally excited emissions. Additionally, we observed the same U-line in both TX Cam and IK Tau, which may originate from the molecule N^(17)O. We analyzed the identified molecular lines using rotational diagrams to determine their excitation temperatures, column densities, and fractional abundances. This information aids in the constructing of reliable astrochemical models for a more detailed examination of the target stars. The narrow component of the SO_(2) line suggests unusual astrophysical phenomena, making IK Tau and AP Lyn particularly intriguing for further investigation to fully understand the physical processes at play in these sources.
文摘The correct evaluation of enterprise technological innovation performance is the premise and foundation to improve the efficiency of enterprise innovation.This paper first puts forward the index system of enterprise technological innovation performance evaluation,then establishes the model of technological innovation performance evaluation,explores the quantitative evaluation of enterprise innovation performance by using the DEA method,and finally makes an empirical study on the technological innovation performance of the TR enterprise.
基金funding from the National Natural Science Foundation of China(Nos.22036002,92043302,21577165,21906176)China Postdoctoral Science Foundation (2018M641495)Strategic Priority Research Program of the Chinese Academy of Sciences(XDPB2002).
文摘Enveloped viruses have been the leading causative agents of viral epidemics in the past decade,including the ongoing coronavirus disease 2019 outbreak.In epidemics caused by enveloped viruses,direct contact is a common route of infection,while indirect transmissions through the environment also contribute to the spread of the disease,although their significance remains controversial.Bridging the knowledge gap regarding the influence of interfacial interactions on the persistence of enveloped viruses in the environment reveals the transmission mechanisms when the virus undergoes mutations and prevents excessive disinfection during viral epidemics.Herein,from the perspective of the driving force,partition efficiency,and viral survivability at interfaces,we summarize the viral and environmental characteristics that affect the environmental transmission of viruses.We expect to provide insights for virus detection,environmental surveillance,and disinfection to limit the spread of severe acute respiratory syndrome coronavirus 2.
基金This work was supported by the National Key Research&Development Program of China(grant Nos.2021YFA1201000 and 2018YFE0117800)the National Natural Science Foundation of China(grant No.82102204)+3 种基金National Natural Science Foundation of China(NSFC)key project(grant No.32030060)NSFC international collaboration key project(grant No.51861135103)The authors also appreciate the support of“the Beijing-Tianjin-Hebei Basic Research Cooperation Project”(19JCZDJC64100)the Science Fund for Creative Research Groups of Nature Science Foundation of Hebei Province(B2021201038).
文摘Enveloped RNA viruses are a group of viruses with an outer membrane derived from a host cell and a genome consisting of ribonucleic acid(RNA).These viruses rely on host cell machinery and organelles to replicate and assemble new virus particles.However,the interaction between viruses and host organelles may be disrupted by nanomaterials,such as gold nanoparticles(AuNPs)with unique physical and chemical properties.In this study,we investigated the effects of AuNPs with different surface charge properties on the subcellular structure and function of mammalian cells,and their effects on two representative enveloped RNA viruses:lentivirus and human coronavirus OC43(HCoV-OC43)antiviral potential.By comparing the subcellular effects of AuNPs with different surface charge properties,we found that treatment with AuNPs with positive surface charges induced more significant disruption of subcellular structures than neutrally charged AuNPs and negatively charged AuNPs,mainly manifested in lysosomes and Cytoskeletal disorders.The antiviral effect of the surface positively charged AuNPs was further evaluated using lentivirus and HCoV-OC43.The results showed that AuNPs had a significant inhibitory effect on both lentivirus and HCoV-OC43 without obvious side effects.In conclusion,our study provides insights into the mechanism of action and biocompatibility of AuNP in biological systems,while supporting the potential of targeting organelle dynamics against enveloped RNA viruses.
基金financially supported by the National Key Research and Development Program of China(Grant No.2022YFC2806102)the National Natural Science Foundation of China(Grant Nos.52171287,52325107)+2 种基金High Tech Ship Research Project of Ministry of Industry and Information Technology(Grant Nos.2023GXB01-05-004-03,GXBZH2022-293)the Science Foundation for Distinguished Young Scholars of Shandong Province(Grant No.ZR2022JQ25)the Taishan Scholars Project(Grant No.tsqn201909063)。
文摘Leakages from subsea oil and gas equipment cause substantial economic losses and damage to marine ecosystem,so it is essential to locate the source of the leak.However,due to the complexity and variability of the marine environment,the signals collected by hydrophone contain a variety of noises,which makes it challenging to extract useful signals for localization.To solve this problem,a hydrophone denoising algorithm is proposed based on variational modal decomposition(VMD)with grey wolf optimization.First,the average envelope entropy is used as the fitness function of the grey wolf optimizer to find the optimal solution for the parameters K andα.Afterward,the VMD algorithm decomposes the original signal parameters to obtain the intrinsic mode functions(IMFs).Subsequently,the number of interrelationships between each IMF and the original signal was calculated,the threshold value was set,and the noise signal was removed to calculate the time difference using the valid signal obtained by reconstruction.Finally,the arrival time difference is used to locate the origin of the leak.The localization accuracy of the method in finding leaks is investigated experimentally by constructing a simulated leak test rig,and the effectiveness and feasibility of the method are verified.
基金supported by the National Natural Science Foundation of China(52078269 and 52325801).
文摘Substantially glazed facades are extensively used in contemporary high-rise buildings to achieve attractive architectural aesthetics.Inherent conflicts exist among architectural aesthetics,building energy consumption,and solar energy harvesting for glazed facades.In this study,we addressed these conflicts by introducing a new dynamic and vertical photovoltaic integrated building envelope(dvPVBE)that offers extraordinary flexibility with weather-responsive slat angles and blind positions,superior architectural aesthetics,and notable energy-saving potential.Three hierarchical control strategies were proposed for different scenarios of the dvPVBE:power generation priority(PGP),natural daylight priority(NDP),and energy-saving priority(ESP).Moreover,the PGP and ESP strategies were further analyzed in the simulation of a dvPVBE.An office room integrated with a dvPVBE was modeled using EnergyPlus.The influence of the dvPVBE in improving the building energy efficiency and corresponding optimal slat angles was investigated under the PGP and ESP control strategies.The results indicate that the application of dvPVBEs in Beijing can provide up to 131%of the annual energy demand of office rooms and significantly increase the annual net energy output by at least 226%compared with static photovoltaic(PV)blinds.The concept of this novel dvPVBE offers a viable approach by which the thermal load,daylight penetration,and energy generation can be effectively regulated.
基金partially funded by AFOSR MURI FA9550-18-502,ONR N00014-18-1-2527,N00014-18-20-1-2093,N00014-20-1-2787supported by the NSF Graduate Research Fellowship under Grant No.DGE-1650604.
文摘Computing tasks may often be posed as optimization problems.The objective functions for real-world scenarios are often nonconvex and/or nondifferentiable.State-of-the-art methods for solving these problems typically only guarantee convergence to local minima.This work presents Hamilton-Jacobi-based Moreau adaptive descent(HJ-MAD),a zero-order algorithm with guaranteed convergence to global minima,assuming continuity of the objective function.The core idea is to compute gradients of the Moreau envelope of the objective(which is"piece-wise convex")with adaptive smoothing parameters.Gradients of the Moreau envelope(i.e.,proximal operators)are approximated via the Hopf-Lax formula for the viscous Hamilton-Jacobi equation.Our numerical examples illustrate global convergence.
基金This work was supported by the National Natural Science Foundation of China(Nos.12005047 and U1832105).
文摘The optical potential ambiguity is a long-standing problem in the analysis of elastic scattering data.For a specific collid-ing system,ambiguous potential families can lead to different behaviors in the nearside and farside scattering components.By contrast,the envelope method can decompose the experimental data into two components with negative and positive deflection angles,respectively.Hence,a question arises as to whether the comparison between the calculated nearside(or farside)component and the derived positive-deflection-angle(or negative-deflection-angle)component can help analyze the potential ambiguity problem.In this study,we conducted a trial application of the envelope method to the potential ambiguity problem.The envelope method was improved by including uncertainties in the experimental data.The colliding systems of 16O+28Si at 215.2 MeV and 12C+12C at 1016 MeV were considered in the analyses.For each colliding system,the angular distribution experimental data were described nearly equally well by two potential sets,one of which is“surface transpar-ent”and the other is refractive.The calculated angular distributions were decomposed into nearside and farside scattering components.Using the improved envelope method,the experimental data were decomposed into the positive-deflection-angle and negative-deflection-angle components,which were then compared with the calculated nearside and farside components.The capability of the envelope method to analyze the potential ambiguities was also discussed.
基金co-supported by the National Science and Technology Major Project,China(No.J2019-I-0015-0014)the National Natural Science Foundation of China(No.52372397).
文摘To design the optimum acceleration control schedule for the Adaptive Cycle Engine(ACE)in the full flight envelope,this paper establishes a direct simulation model of the ACE transient state.In this model,geometric parameters are used to replace the component state parameters.The corresponding relationship between geometric parameters and component state parameters is determined by sensitivity analysis.The geometric variables are controlled when the geometric adjustment speed exceeds the limit,and at the same time the corresponding component state parameters are iterated.The gradient optimization algorism is used to optimize the ground acceleration process of ACE,and the control schedule in terms of operating point of compression components and corrected acceleration rate is used as the full-envelope acceleration control schedule based on the similarity principle.The acceleration control schedules of the triple-bypass mode and the double-bypass mode are designed in this paper.The acceleration processes under various flight conditions are simulated using the acceleration control schedules.Compared with the acceleration process with the linear geometric adjustment schedule,the acceleration performance of ACE is improved by the acceleration control schedule,with the impulse of the acceleration process of the triple-bypass mode being increased by 8.7%-12.3% and the impulse of the double-bypass mode acceleration process being increased by 11.8%-14.1%.
基金the financial support of the National Key R&D Program of China(2021YFC3000701)the China Seismic Experimental Site in Sichuan-Yunnan(CSES-SY)for providing data for this study.
文摘The development of machine learning technology enables more robust real-time earthquake monitoring through automated implementations. However, the application of machine learning to earthquake location problems faces challenges in regions with limited available training data. To address the issues of sparse event distribution and inaccurate ground truth in historical seismic datasets, we expand the training dataset by using a large number of synthetic envelopes that closely resemble real data and build an earthquake location model named ENVloc. We propose an envelope-based machine learning workflow for simultaneously determining earthquake location and origin time. The method eliminates the need for phase picking and avoids the accumulation of location errors resulting from inaccurate picking results. In practical application, ENVloc is applied to several data intercepted at different starting points. We take the starting point of the time window corresponding to the highest prediction probability value as the origin time and save the predicted result as the earthquake location. We apply ENVloc to observed data acquired in the southern Sichuan Basin, China, between September 2018 and March 2019. The results show that the average difference with the catalog in latitude, longitude, depth, and origin time is 0.02°,0.02°, 2 km, and 1.25 s, respectively. These suggest that our envelope-based method provides an efficient and robust way to locate earthquakes without phase picking, and can be used in earthquake monitoring in near-real time.
基金supported in part by the Natour Project Joint Post-Graduate Study Programme in Ecotourism and Nature Guiding(619157-EPP-1-2020-1-ES-EPPKA2-CBHE-JP).
文摘The tourism industry is economically very important.According to the World Travel Tourism Council,in 2019,the tourism industry accounted for a quarter of all new jobs created worldwide,10.3%of all jobs,and 9.6×1012 USD of the global gross domestic product.This study aimed to calculate the tourism efficiency index for different Latin American countries from 2010 to 2021 using data envelopment analysis,which analyzes the relationships between input variables(including the number of employees in the tourism industry and the number of hotel-type establishments)and output variables(including tourism expenditures in other countries and public social expenditures in recreation and culture per capita).Additionally,this study aimed to identify the countries with greater tourism development and the factors that may affect the development of the tourism industry through the stochastic frontier production function.The results of the tourism efficiency index for Central America(including Costa Rica,Dominica,El Salvador,Honduras,Mexico,and Panama)and South America(including Argentina,Brazil,Chile,Colombia,Ecuador,Paraguay,Peru,and Uruguay)exhibited different trends.However,after the global health crisis,the tourism industry recovered,showing new opportunities to promote sustainability.The results of the stochastic frontier production function demonstrated that countries with higher levels of inbound and outbound tourism,contribution of tourism to the economy,natural resources,and literacy rate exhibited more efficient tourism industry,whereas countries with higher pollution levels exhibited less efficient tourism industry.The findings of this study could allow us to formulate suitable public policies to promote tourism,maintain natural resources,and diversify these sectors with more inclusive programmes that can facilitate growth and benefit vulnerable communities.
基金Supported by the Shanghai Municipal Commission of Health and Family Planning,No.PKJ2018-Y05.
文摘BACKGROUND Occult hepatitis B infection(OBI)is characterized by the detection of hepatitis B virus(HBV)DNA in serum(usually HBV DNA<200 IU/mL)or the liver but negativity for hepatitis B surface antigen(HBsAg).The diagnosis of OBI relies on the sensitivity of assays used in the detection of HBV DNA and HBsAg.HBsAg assays with inadequate sensitivity or inability to detect HBV S variants may lead to misdiagnosis of OBI in people with overt HBV infection.CASE SUMMARY We report a HBsAg-negative but hepatitis B envelope antigen-positive patient who had a significant HBV DNA level.The patient was initially diagnosed as having OBI.However,sequence analysis revealed a unique insertion of amino acid residues at positions 120-124 in the S protein,which affects the formation of a disulfide bond that is associated with the formation of a loop.It is well known that there is an overlap between the S protein and Pol protein.We found that this new insertion site occurred in polymerase/reverse transcriptase domain,indi-cating that this insertion might be involved in HBV pathogenicity.The patient was finally diagnosed with a false OBI.CONCLUSION An insertion of amino acid residues at positions 120-124 of the S protein affects the formation of immunodominant epitopes and results in negative HBsAg levels.
