MUKAIDONO proposed and systematically investigated the theory of regular ternary logic functions that meets the need of uncertain inferences. The enumeration of ternary logic functions is very complicated and several ...MUKAIDONO proposed and systematically investigated the theory of regular ternary logic functions that meets the need of uncertain inferences. The enumeration of ternary logic functions is very complicated and several results have been obtained only in the case where the number of variables is less than 7. In this letter we offer a new possible way to solve the prob-展开更多
Enumerating the isomorphism or equivalence classes of several types of graph coverings is one of the central research topics in enumerative topological graph theory.A covering projection p from a Cayley graph Cay(Г,X...Enumerating the isomorphism or equivalence classes of several types of graph coverings is one of the central research topics in enumerative topological graph theory.A covering projection p from a Cayley graph Cay(Г,X)onto another Cayley graph Cay(Q,y)is called typical if the function p:Г→Q on the vertex sets is a group epimorphism.A typical covering is called abelian(or circulant,respectively)if its covering graph is a Cayley graph on an abelism(or a cyclic,respectively)group.Recently,the equivalence classes of connected abelian typical prime-fold coverings of a circulant graph are enumerated.As a continuation of this work,we enumerate the equivalence classes of connected abelian typical cube-free fold coverings of a circulant graph.展开更多
In this study, ozone gas was applied to samples of durum wheat stored in four experimental groups (durum wheat without any treatment for comparison, durum wheat treated with ozone, purified durum wheat, and purified d...In this study, ozone gas was applied to samples of durum wheat stored in four experimental groups (durum wheat without any treatment for comparison, durum wheat treated with ozone, purified durum wheat, and purified durum wheat treated with ozone). Two groups were treated with ozone gas at 3 ppm concentration for 1 hour. Groups were then placed in air-tight glass jars and stored for 6 months at variable temperatures between 24.7°C to 34.8°C. Microbiological (total count bacteria, yeast/molds and coliform) and physical properties (moisture, color and ash) evaluated. Ozone application statistically caused a significant reduction in the numbers of bacteria, yeast, molds and coliforms. Ozone application, washing process and storage temperature are the major factors affecting the microbial counts. No significant differences were determined in moisture and ash contents of samples after ozone treatment. The color measurement results showed that color values of wheat samples were affected by ozone treatment, storage and washing.展开更多
Recently,linear codes with a few weights have been extensively studied due to their applications in secret sharing schemes,constant composition codes,strongly regular graphs and so on.In this paper,based on the Weil s...Recently,linear codes with a few weights have been extensively studied due to their applications in secret sharing schemes,constant composition codes,strongly regular graphs and so on.In this paper,based on the Weil sums,several classes of two-weight or three-weight linear codes are presented by choosing a proper defining set,and their weight enumerators and complete weight enumerators are determined.Furthermore,these codes are proven to be minimal.By puncturing these linear codes,two classes of two-weight projective codes are obtained,and the parameters of the corresponding strongly regular graph are given.This paper generalizes the results of[7].展开更多
A map is bisingular if each edge is either a loop (This paper only considers planar loop) or an isthmus (i.e., on the boundary of the same face). This paper studies the number of rooted bisingular maps on the sphere a...A map is bisingular if each edge is either a loop (This paper only considers planar loop) or an isthmus (i.e., on the boundary of the same face). This paper studies the number of rooted bisingular maps on the sphere and the torus, and also presents formulae for such maps with three parameters: the root-valency, the number of isthmus, and the number of planar loops.展开更多
It is well known that singular maps(i.e.,those have only one face on a surface)play a key role in the theory of up-embeddability of graphs.In this paper the number of rooted singular maps on the Klein bottle is studie...It is well known that singular maps(i.e.,those have only one face on a surface)play a key role in the theory of up-embeddability of graphs.In this paper the number of rooted singular maps on the Klein bottle is studied.An explicit form of the enumerating function according to the root-valency and the size of the map is determined.Further,an expression of the vertex partition function is also found.展开更多
In this paper we give a formula for enumerating the equivalent classes of orderly labeled Hamiltonian graphs under group D. and two algorithms for constructing these equivalent classes and all nonisomorphic Hamiltonia...In this paper we give a formula for enumerating the equivalent classes of orderly labeled Hamiltonian graphs under group D. and two algorithms for constructing these equivalent classes and all nonisomorphic Hamiltonian graphs. Some computational results obtained by microcomputers are listed.展开更多
The subcarrier allocation problem in cognitive radio（CR）networks with multi-user orthogonal frequency-division multiplexing（OFDM）and distributed antenna is analyzed and modeled for the flat fading channel and the ...The subcarrier allocation problem in cognitive radio（CR）networks with multi-user orthogonal frequency-division multiplexing（OFDM）and distributed antenna is analyzed and modeled for the flat fading channel and the frequency selective channel,where the constraint on the secondary user（SU）to protect the primary user（PU）is that the total throughput of each PU must be above the given threshold instead of the ＂interference temperature＂.According to the features of different types of channels,the optimal subcarrier allocation schemes are proposed to pursue efficiency（or maximal throughput）,using the branch and bound algorithm and the 0-1 implicit enumeration algorithm.Furthermore,considering the tradeoff between efficiency and fairness,the optimal subcarrier allocation schemes with fairness are proposed in different fading channels,using the pegging algorithm.Extensive simulation results illustrate the significant performance improvement of the proposed subcarrier allocation schemes compared with the existing ones in different scenarios.展开更多
The Ludox-QPS method is a newly developed technique,which combines the Ludox HS 40 density centrifugation and quantitative protargol stain,to enumerate marine ciliates with good taxonomic resolution.We tested the meth...The Ludox-QPS method is a newly developed technique,which combines the Ludox HS 40 density centrifugation and quantitative protargol stain,to enumerate marine ciliates with good taxonomic resolution.We tested the method for simultaneous enumeration of diatoms,protozoa and meiobenthos and compared its extraction efficiency for meiobenthos with that of the routine Ludox-TM centrifugation and a modified protocol using Ludox HS 40.We conducted the evaluation with a sample size of 8.3 ml each from sandy,muddy-sand and muddy sediments collected from the intertidal area of the Yellow Sea in summer 2006 and spring 2007.The Ludox-QPS method not only produced high extraction efficiencies of 97±1.3% for diatoms and 97.6±0.8% for ciliates,indicating a reliable enumeration for eukaryotic microbenthos,but also produced excellent extraction efficiencies of on average 97.3% for total meiobenthos,97.9% for nematodes and 97.8% for copepods from sands,muddy sands and mud.By contrast,the routine Ludox-TM centrifugation obtained only about 74% of total meiobenthos abundance with one extraction cycle,and the modified Ludox HS 40 centrifugation yielded on average 93% of total meiobenthos:89.4±2.0% from sands,93±4.1% from muddy sands and 97.1±3.0% from mud.Apart from the sediment type,sample volume was another important factor affecting the extraction efficiency for meiobenthos.The extraction rate was increased to about 96.4% when using the same modified Ludox centrifugation for a 4 ml sediment sample.Besides the excellent extraction efficiency,the Ludox-QPS method obtained higher abundances of meiobenthos,in particular nematodes,than the routine Ludox centrifugation,which frequently resulted in an uncertain loss of small meiobenthos during the sieving process.Statistical analyses demonstrated that there were no significant differences between the meiobenthos communities revealed by the Ludox-QPS method and the modified Ludox HS 40 centrifugation,showing the high efficiency of the Ludox-QPS method for simultaneous enumeration of diatom,protozoa and meiobenthos.Moreover,the comparatively high taxonomic resolution of the method,especially for diatoms and ciliates,makes it feasible to investigate microbial ecology at community level.展开更多
AIM: To establish a scoring system for predicting the incidence of postoperative complications and mortality in general surgery based on the physiological and operative severity score for the enumeration of mortality ...AIM: To establish a scoring system for predicting the incidence of postoperative complications and mortality in general surgery based on the physiological and operative severity score for the enumeration of mortality and morbidity (POSSUM), and to evaluate its efficacy. METHODS: Eighty-four patients with postoperative complications or death and 172 patients without postoperative complications, who underwent surgery in our department during the previous 2 years, were retrospectively analyzed by logistic regression. Fifteen indexes were investigated including age, cardiovascular function, respiratory function, blood test results, endocrine function, central nervous system function, hepatic function, renal function, nutritional status, extent of operative trauma, and course of anesthesia. Modified POSSUM (M-POSSUM) was developed using significant risk factors with its efficacy evaluated. RESULTS: The significant risk factors were found to be age, cardiovascular function, respiratory function, hepatic function, renal function, blood test results, endocrine function, nutritional status, duration of operation, intraoperative blood loss, and course of anesthesia. These factors were all included in the scoring system. There were significant differences in the scores between the patients with and without postoperative complications, between the patients died and survived with complications, and between the patients died and survived without complications. The receiver operating characteristic curves showed that the M-POSSUM could accurately predict postoperative complications and mortality.CONCLUSION: M-POSSUM correlates well with postoperative complications and mortality, and is more accurate than POSSUM.展开更多
A quantitative protocol for the rapid analysis of Microcystis cells and colonies in lake sediment was developed using a modified flow cytometer, the CytoSense. For cell enumeration, diluted sediment samples containing...A quantitative protocol for the rapid analysis of Microcystis cells and colonies in lake sediment was developed using a modified flow cytometer, the CytoSense. For cell enumeration, diluted sediment samples containing Microcystis were processed with sonication to disintegrate colonies into single cells. An optimized procedure suggested that 5 mg dw （dry weight）/mL dilution combined with 200 W x 2 min sonication yielded the highest counting efficiency. Under the optimized determination conditions, the quantification limit of this protocol was 3.3x104 cells/g dw. For colony analysis, Microcystis were isolated from the sediment by filtration. Colony lengths measured by flow cytometry were similar to those measured by microscopy for the size range of one single cell to almost 400 ~tm in length. Moreover, the relationship between colony size and cell number was determined for three Microcystis species, including Microcystisflos-aquae, M. aeruginosa and M. wessenbergii. Regression formulas were used to calculate the cell numbers in different- sized colonies. The developed protocol was applied to field sediment samples from Lake Taihu. The results indicated the potential and applicability of flow cytometry as a tool for the rapid analysis of benthic Microcystis. This study provided a new capability for the high frequency monitoring of benthic overwintering and population dynamics of this bloom-forming cyanobacterium.展开更多
Hilbert problem 15 required understanding Schubert's book.In this book,reducing to degenerate cases was one of the main methods for enumeration.We found that nonstandard analysis is a suitable tool for making rigo...Hilbert problem 15 required understanding Schubert's book.In this book,reducing to degenerate cases was one of the main methods for enumeration.We found that nonstandard analysis is a suitable tool for making rigorous of Schubert's proofs of some results,which used degeneration method,but are obviously not rigorous.In this paper,we give a rigorous proof for Example 4 in Schubert's book,Chapter 1.§4 according to his idea.This shows that Schubert's intuitive idea is correct,but to make it rigorous a lot of work should be done.展开更多
In this paper,we give rigorous justification of the ideas put forward in§20,Chapter 4 of Schubert’s book;a section that deals with the enumeration of conics in space.In that section,Schubert introduced two degen...In this paper,we give rigorous justification of the ideas put forward in§20,Chapter 4 of Schubert’s book;a section that deals with the enumeration of conics in space.In that section,Schubert introduced two degenerate conditions about conics,i.e.,the double line and the two intersection lines.Using these two degenerate conditions,he obtained all relations regarding the following three conditions:conics whose planes pass through a given point,conics intersecting with a given line,and conics which are tangent to a given plane.We use the language of blow-ups to rigorously treat the two degenerate conditions and prove all formulas about degenerate conditions stemming from Schubert’s idea.展开更多
The concept of graphlike manifolds was presented in [1] and the problem of counting the homeomorphic classes of graphlike manifolds has been studied in a series of articles, e.g., [1～8]. In this paper we suggest an a...The concept of graphlike manifolds was presented in [1] and the problem of counting the homeomorphic classes of graphlike manifolds has been studied in a series of articles, e.g., [1～8]. In this paper we suggest an approach based on the graph colouring, Abelian group and the combinatorial enumeration method.展开更多
Airborne bacteria were measured when a dust storm passed Beijing in spring 2012 with a focus on cell concentration, viability and TSA- and R2A-cultured strain composition. The concentration varied at an order of 10^7 ...Airborne bacteria were measured when a dust storm passed Beijing in spring 2012 with a focus on cell concentration, viability and TSA- and R2A-cultured strain composition. The concentration varied at an order of 10^7 cells/m3 with dust loading （demonstrated with PM10） and they had a very close correlation （RT2 = 0.91, p 〈 0.01）. At the time of highest PM10 of 652 μg/m3, the bacterial concentration reached 1.4 × 10^8 cells/m3, which was larger than that before and after the dust event by one order. Bacterial viability, the ratio of number concentration of viable cells to total cells, was 32%-64% and smaller in the dust plume than that before the dust arrival. Bacterial strains from the culture ranged between 2.5 × 10^4 and 4.6 × 10^5 CFU/m3 and no correlation with PM10 was determined. Their composition was different before and after the dust arrival according to 16S rRNA gene sequences and strains belong to Actinomycetes and Firmicutes were the majority in the dust samples.展开更多
Hilbert Problem 15 required an understanding of Schubert’s book[1],both its methods and its results.In this paper,following his idea,we prove that the formulas in§6,§7,§10,about the incidence of points...Hilbert Problem 15 required an understanding of Schubert’s book[1],both its methods and its results.In this paper,following his idea,we prove that the formulas in§6,§7,§10,about the incidence of points,lines and planes,are all correct.As an application,we prove formulas 8 and 9 in§12,which are frequently used in his book.展开更多
Rainwater contains substantial bacteria and rain is an efficient pathway for the dissemination of bacteria from the atmosphere to land and water surfaces.However,quantitative information on rainwater bacteria is very ...Rainwater contains substantial bacteria and rain is an efficient pathway for the dissemination of bacteria from the atmosphere to land and water surfaces.However,quantitative information on rainwater bacteria is very limited due to the lack of a reliable method.In this study,the epifluorescence microscopy enumeration with the LIVE/DEAD BacLight Bacterial Viability Kit stain was verified to quantify the abundance of viable and non-viable bacterial cells in rainwater,with the 4＇,6-diamidino-2-phenylindole（DAPI） stain for the reference of total cell counts.Results showed that the total counts of bacterial cells by LIVE/DEAD BacLight staining were consistent with those by DAPI staining,and the average detection efficiency was（109 ± 29）%.The ratio of cell count with glutaraldehyde fixation to that without fixation was（106 ± 5）%on average.The bacterial concentration in negative control was usually an order of magnitude lower than that in rainwater samples.However,in case of small precipitation,the abundance in negative control could be more than that in rainwater samples.These results indicate that the enumeration with LIVE/DEAD BacLight bacterial viability assay coupled with glutaraldehyde fixation and careful negative control investigation is an approach applicable to the measurement of the concentration and viability of bacterial cells in rainwater.展开更多
As one of the most widely used assays in biological research,an enumeration of the bacterial cell colonies is an important but time-consuming and labor-intensive process.To speed up the colony counting,a machine learn...As one of the most widely used assays in biological research,an enumeration of the bacterial cell colonies is an important but time-consuming and labor-intensive process.To speed up the colony counting,a machine learning method is presented for counting the colony forming units(CFUs),which is referred to as CFUCounter.This cellcounting program processes digital images and segments bacterial colonies.The algorithm combines unsupervised machine learning,iterative adaptive thresholding,and local-minima-based watershed segmentation to enable an accurate and robust cell counting.Compared to a manual counting method,CFUCounter supports color-based CFU classification,allows plates containing heterologous colonies to be counted individually,and demonstrates overall performance(slope 0.996,SD 0.013,95%CI:0.97–1.02,p value<1e-11,r=0.999)indistinguishable from the gold standard of point-and-click counting.This CFUCounter application is open-source and easy to use as a unique addition to the arsenal of colony-counting tools.展开更多
Let Γm，n^* denote all m × n strongly connected bipartite tournaments and a(m, n) the maximal integer k such that every m × n bipartite tournament contains at least a k × k transitive bipartite subtour...Let Γm，n^* denote all m × n strongly connected bipartite tournaments and a(m, n) the maximal integer k such that every m × n bipartite tournament contains at least a k × k transitive bipartite subtournament. Let t ( m, n, k, l ) = max{t( Tm,n，k, l ) : Tm,n∈Γm，n^*}, where t ( Tm,n, k, l ) is the number of k × l(k≥2,l≥2) transitive bipartite subtournaments contained in Tm,n∈Γm，n^*. We obtain a method of graph theory for solving some integral programmings, investigate the upper bounds of a(m,n) and obtain t (m,n, k，l).展开更多
文摘MUKAIDONO proposed and systematically investigated the theory of regular ternary logic functions that meets the need of uncertain inferences. The enumeration of ternary logic functions is very complicated and several results have been obtained only in the case where the number of variables is less than 7. In this letter we offer a new possible way to solve the prob-
基金The work was partially supported by the Korean-Russian bilateral project.The first author was supported by the Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Education(grant 2018R1D1A1B05048450),Korea.
文摘Enumerating the isomorphism or equivalence classes of several types of graph coverings is one of the central research topics in enumerative topological graph theory.A covering projection p from a Cayley graph Cay(Г,X)onto another Cayley graph Cay(Q,y)is called typical if the function p:Г→Q on the vertex sets is a group epimorphism.A typical covering is called abelian(or circulant,respectively)if its covering graph is a Cayley graph on an abelism(or a cyclic,respectively)group.Recently,the equivalence classes of connected abelian typical prime-fold coverings of a circulant graph are enumerated.As a continuation of this work,we enumerate the equivalence classes of connected abelian typical cube-free fold coverings of a circulant graph.
文摘In this study, ozone gas was applied to samples of durum wheat stored in four experimental groups (durum wheat without any treatment for comparison, durum wheat treated with ozone, purified durum wheat, and purified durum wheat treated with ozone). Two groups were treated with ozone gas at 3 ppm concentration for 1 hour. Groups were then placed in air-tight glass jars and stored for 6 months at variable temperatures between 24.7°C to 34.8°C. Microbiological (total count bacteria, yeast/molds and coliform) and physical properties (moisture, color and ash) evaluated. Ozone application statistically caused a significant reduction in the numbers of bacteria, yeast, molds and coliforms. Ozone application, washing process and storage temperature are the major factors affecting the microbial counts. No significant differences were determined in moisture and ash contents of samples after ozone treatment. The color measurement results showed that color values of wheat samples were affected by ozone treatment, storage and washing.
基金supported by the Natural Science Foundation of China (No.11901062)the Sichuan Natural Science Foundation (No.2024NSFSC0417)。
文摘Recently,linear codes with a few weights have been extensively studied due to their applications in secret sharing schemes,constant composition codes,strongly regular graphs and so on.In this paper,based on the Weil sums,several classes of two-weight or three-weight linear codes are presented by choosing a proper defining set,and their weight enumerators and complete weight enumerators are determined.Furthermore,these codes are proven to be minimal.By puncturing these linear codes,two classes of two-weight projective codes are obtained,and the parameters of the corresponding strongly regular graph are given.This paper generalizes the results of[7].
基金Supported by fifteenth programming of Central University for Nationalities, NNSFC under Grant No.10271048 and 19831080
文摘A map is bisingular if each edge is either a loop (This paper only considers planar loop) or an isthmus (i.e., on the boundary of the same face). This paper studies the number of rooted bisingular maps on the sphere and the torus, and also presents formulae for such maps with three parameters: the root-valency, the number of isthmus, and the number of planar loops.
基金the National Natural Science Foundation of China(1 983 1 0 80 )
文摘It is well known that singular maps(i.e.,those have only one face on a surface)play a key role in the theory of up-embeddability of graphs.In this paper the number of rooted singular maps on the Klein bottle is studied.An explicit form of the enumerating function according to the root-valency and the size of the map is determined.Further,an expression of the vertex partition function is also found.
基金Project supported by National Natural Foundation of China
文摘In this paper we give a formula for enumerating the equivalent classes of orderly labeled Hamiltonian graphs under group D. and two algorithms for constructing these equivalent classes and all nonisomorphic Hamiltonian graphs. Some computational results obtained by microcomputers are listed.
基金The National Natural Science Foundation of China(No.60832009)Beijing Municipal Natural Science Foundation(No.4102044)National Major Science & Technology Project(No.2009ZX03003-003-01)
文摘The subcarrier allocation problem in cognitive radio（CR）networks with multi-user orthogonal frequency-division multiplexing（OFDM）and distributed antenna is analyzed and modeled for the flat fading channel and the frequency selective channel,where the constraint on the secondary user（SU）to protect the primary user（PU）is that the total throughput of each PU must be above the given threshold instead of the ＂interference temperature＂.According to the features of different types of channels,the optimal subcarrier allocation schemes are proposed to pursue efficiency（or maximal throughput）,using the branch and bound algorithm and the 0-1 implicit enumeration algorithm.Furthermore,considering the tradeoff between efficiency and fairness,the optimal subcarrier allocation schemes with fairness are proposed in different fading channels,using the pegging algorithm.Extensive simulation results illustrate the significant performance improvement of the proposed subcarrier allocation schemes compared with the existing ones in different scenarios.
基金Supported by the Knowledge Innovation Program of Chinese Academy of Sciences (No.KZCX2-YW-417)the National Natural Science Foundation of China (No.40576072+1 种基金40706047)the "100 Talents Project" of the Chinese Academy of Sciences
文摘The Ludox-QPS method is a newly developed technique,which combines the Ludox HS 40 density centrifugation and quantitative protargol stain,to enumerate marine ciliates with good taxonomic resolution.We tested the method for simultaneous enumeration of diatoms,protozoa and meiobenthos and compared its extraction efficiency for meiobenthos with that of the routine Ludox-TM centrifugation and a modified protocol using Ludox HS 40.We conducted the evaluation with a sample size of 8.3 ml each from sandy,muddy-sand and muddy sediments collected from the intertidal area of the Yellow Sea in summer 2006 and spring 2007.The Ludox-QPS method not only produced high extraction efficiencies of 97±1.3% for diatoms and 97.6±0.8% for ciliates,indicating a reliable enumeration for eukaryotic microbenthos,but also produced excellent extraction efficiencies of on average 97.3% for total meiobenthos,97.9% for nematodes and 97.8% for copepods from sands,muddy sands and mud.By contrast,the routine Ludox-TM centrifugation obtained only about 74% of total meiobenthos abundance with one extraction cycle,and the modified Ludox HS 40 centrifugation yielded on average 93% of total meiobenthos:89.4±2.0% from sands,93±4.1% from muddy sands and 97.1±3.0% from mud.Apart from the sediment type,sample volume was another important factor affecting the extraction efficiency for meiobenthos.The extraction rate was increased to about 96.4% when using the same modified Ludox centrifugation for a 4 ml sediment sample.Besides the excellent extraction efficiency,the Ludox-QPS method obtained higher abundances of meiobenthos,in particular nematodes,than the routine Ludox centrifugation,which frequently resulted in an uncertain loss of small meiobenthos during the sieving process.Statistical analyses demonstrated that there were no significant differences between the meiobenthos communities revealed by the Ludox-QPS method and the modified Ludox HS 40 centrifugation,showing the high efficiency of the Ludox-QPS method for simultaneous enumeration of diatom,protozoa and meiobenthos.Moreover,the comparatively high taxonomic resolution of the method,especially for diatoms and ciliates,makes it feasible to investigate microbial ecology at community level.
文摘AIM: To establish a scoring system for predicting the incidence of postoperative complications and mortality in general surgery based on the physiological and operative severity score for the enumeration of mortality and morbidity (POSSUM), and to evaluate its efficacy. METHODS: Eighty-four patients with postoperative complications or death and 172 patients without postoperative complications, who underwent surgery in our department during the previous 2 years, were retrospectively analyzed by logistic regression. Fifteen indexes were investigated including age, cardiovascular function, respiratory function, blood test results, endocrine function, central nervous system function, hepatic function, renal function, nutritional status, extent of operative trauma, and course of anesthesia. Modified POSSUM (M-POSSUM) was developed using significant risk factors with its efficacy evaluated. RESULTS: The significant risk factors were found to be age, cardiovascular function, respiratory function, hepatic function, renal function, blood test results, endocrine function, nutritional status, duration of operation, intraoperative blood loss, and course of anesthesia. These factors were all included in the scoring system. There were significant differences in the scores between the patients with and without postoperative complications, between the patients died and survived with complications, and between the patients died and survived without complications. The receiver operating characteristic curves showed that the M-POSSUM could accurately predict postoperative complications and mortality.CONCLUSION: M-POSSUM correlates well with postoperative complications and mortality, and is more accurate than POSSUM.
基金supported by the National Basic Research Program (973) of China (No. 2008CB418006)the National Special Program of Water Environment (No.2009ZX07106-001-002)+1 种基金the National Natural Science Foundation of China (No. 31070355)the National Major Science and Technology Program for Water Pollution Control and Treatment (No. 2009ZX07101-013)
文摘A quantitative protocol for the rapid analysis of Microcystis cells and colonies in lake sediment was developed using a modified flow cytometer, the CytoSense. For cell enumeration, diluted sediment samples containing Microcystis were processed with sonication to disintegrate colonies into single cells. An optimized procedure suggested that 5 mg dw （dry weight）/mL dilution combined with 200 W x 2 min sonication yielded the highest counting efficiency. Under the optimized determination conditions, the quantification limit of this protocol was 3.3x104 cells/g dw. For colony analysis, Microcystis were isolated from the sediment by filtration. Colony lengths measured by flow cytometry were similar to those measured by microscopy for the size range of one single cell to almost 400 ~tm in length. Moreover, the relationship between colony size and cell number was determined for three Microcystis species, including Microcystisflos-aquae, M. aeruginosa and M. wessenbergii. Regression formulas were used to calculate the cell numbers in different- sized colonies. The developed protocol was applied to field sediment samples from Lake Taihu. The results indicated the potential and applicability of flow cytometry as a tool for the rapid analysis of benthic Microcystis. This study provided a new capability for the high frequency monitoring of benthic overwintering and population dynamics of this bloom-forming cyanobacterium.
文摘Hilbert problem 15 required understanding Schubert's book.In this book,reducing to degenerate cases was one of the main methods for enumeration.We found that nonstandard analysis is a suitable tool for making rigorous of Schubert's proofs of some results,which used degeneration method,but are obviously not rigorous.In this paper,we give a rigorous proof for Example 4 in Schubert's book,Chapter 1.§4 according to his idea.This shows that Schubert's intuitive idea is correct,but to make it rigorous a lot of work should be done.
基金partially supported by National Center for Mathematics and Interdisciplinary Sciences,CAS。
文摘In this paper,we give rigorous justification of the ideas put forward in§20,Chapter 4 of Schubert’s book;a section that deals with the enumeration of conics in space.In that section,Schubert introduced two degenerate conditions about conics,i.e.,the double line and the two intersection lines.Using these two degenerate conditions,he obtained all relations regarding the following three conditions:conics whose planes pass through a given point,conics intersecting with a given line,and conics which are tangent to a given plane.We use the language of blow-ups to rigorously treat the two degenerate conditions and prove all formulas about degenerate conditions stemming from Schubert’s idea.
文摘The concept of graphlike manifolds was presented in [1] and the problem of counting the homeomorphic classes of graphlike manifolds has been studied in a series of articles, e.g., [1～8]. In this paper we suggest an approach based on the graph colouring, Abelian group and the combinatorial enumeration method.
基金the support of the Sumitomo Environmental Foundation(103096) of 2011–2012the Grant-in-Aid for Exploratory Research of JSPS(15K12192)the National Natural Science Foundation of China(No: 31470232)
文摘Airborne bacteria were measured when a dust storm passed Beijing in spring 2012 with a focus on cell concentration, viability and TSA- and R2A-cultured strain composition. The concentration varied at an order of 10^7 cells/m3 with dust loading （demonstrated with PM10） and they had a very close correlation （RT2 = 0.91, p 〈 0.01）. At the time of highest PM10 of 652 μg/m3, the bacterial concentration reached 1.4 × 10^8 cells/m3, which was larger than that before and after the dust event by one order. Bacterial viability, the ratio of number concentration of viable cells to total cells, was 32%-64% and smaller in the dust plume than that before the dust arrival. Bacterial strains from the culture ranged between 2.5 × 10^4 and 4.6 × 10^5 CFU/m3 and no correlation with PM10 was determined. Their composition was different before and after the dust arrival according to 16S rRNA gene sequences and strains belong to Actinomycetes and Firmicutes were the majority in the dust samples.
基金partially supported by National Center for Mathematics and Interdisciplinary Sciences,CAS。
文摘Hilbert Problem 15 required an understanding of Schubert’s book[1],both its methods and its results.In this paper,following his idea,we prove that the formulas in§6,§7,§10,about the incidence of points,lines and planes,are all correct.As an application,we prove formulas 8 and 9 in§12,which are frequently used in his book.
基金supported by a Grant-in-Aid for Challenging Exploratory Research(No.15K12192)from the Japan Society for the Promotion of Science(JSPS)The State Scholarship Fund of Chinese Scholarship Council(CSC No.201406010350)provides Mr.Wei Hu the scholarship for his study and stay at the Prefectural University of Kumamoto
文摘Rainwater contains substantial bacteria and rain is an efficient pathway for the dissemination of bacteria from the atmosphere to land and water surfaces.However,quantitative information on rainwater bacteria is very limited due to the lack of a reliable method.In this study,the epifluorescence microscopy enumeration with the LIVE/DEAD BacLight Bacterial Viability Kit stain was verified to quantify the abundance of viable and non-viable bacterial cells in rainwater,with the 4＇,6-diamidino-2-phenylindole（DAPI） stain for the reference of total cell counts.Results showed that the total counts of bacterial cells by LIVE/DEAD BacLight staining were consistent with those by DAPI staining,and the average detection efficiency was（109 ± 29）%.The ratio of cell count with glutaraldehyde fixation to that without fixation was（106 ± 5）%on average.The bacterial concentration in negative control was usually an order of magnitude lower than that in rainwater samples.However,in case of small precipitation,the abundance in negative control could be more than that in rainwater samples.These results indicate that the enumeration with LIVE/DEAD BacLight bacterial viability assay coupled with glutaraldehyde fixation and careful negative control investigation is an approach applicable to the measurement of the concentration and viability of bacterial cells in rainwater.
基金This research was funded by a VPR Special Research Grant entitled Potential of a Site-Specific DNA Interstrand Crosslink.
文摘As one of the most widely used assays in biological research,an enumeration of the bacterial cell colonies is an important but time-consuming and labor-intensive process.To speed up the colony counting,a machine learning method is presented for counting the colony forming units(CFUs),which is referred to as CFUCounter.This cellcounting program processes digital images and segments bacterial colonies.The algorithm combines unsupervised machine learning,iterative adaptive thresholding,and local-minima-based watershed segmentation to enable an accurate and robust cell counting.Compared to a manual counting method,CFUCounter supports color-based CFU classification,allows plates containing heterologous colonies to be counted individually,and demonstrates overall performance(slope 0.996,SD 0.013,95%CI:0.97–1.02,p value<1e-11,r=0.999)indistinguishable from the gold standard of point-and-click counting.This CFUCounter application is open-source and easy to use as a unique addition to the arsenal of colony-counting tools.
文摘Let Γm，n^* denote all m × n strongly connected bipartite tournaments and a(m, n) the maximal integer k such that every m × n bipartite tournament contains at least a k × k transitive bipartite subtournament. Let t ( m, n, k, l ) = max{t( Tm,n，k, l ) : Tm,n∈Γm，n^*}, where t ( Tm,n, k, l ) is the number of k × l(k≥2,l≥2) transitive bipartite subtournaments contained in Tm,n∈Γm，n^*. We obtain a method of graph theory for solving some integral programmings, investigate the upper bounds of a(m,n) and obtain t (m,n, k，l).
