A simple HPLC method was established for the determination of entrapment efficiency of a new 5-FPE liposome formulation. Chromatographic separation was performed on a Kromasil 100 A C18 column （350 mm×4.6 mm, 5 ...A simple HPLC method was established for the determination of entrapment efficiency of a new 5-FPE liposome formulation. Chromatographic separation was performed on a Kromasil 100 A C18 column （350 mm×4.6 mm, 5 μm）. The mobile phase was consisted of methanol-water （58：42, v/v）. The flow rate of mobile phase was set at 0.8 mL/min. The UV detection wavelength was 271 nm, and the column temperature was 30 ℃. The linear range of 5-FPE was from 0.8-12.8 μg/mL, r = 0.9999. The RSD of intm-day and inter-day precision were less than 2.97%. The average recovery was from 96.8%-104.6% with RSD less than 2.24%. The method was simple, rapid, accurate, and sensitive. It is suitable for the determination of entrapment efficiency of the 5-FPE liposome formulation.展开更多
This report studied on pharmaceutical characteristics of the stealth liposome containing dau-norubicin (DNR). The shape, size, entrapment efficiency and stability of the daunorubicin stealth liposomes (DNRSL) were exa...This report studied on pharmaceutical characteristics of the stealth liposome containing dau-norubicin (DNR). The shape, size, entrapment efficiency and stability of the daunorubicin stealth liposomes (DNRSL) were examined. Visible spectrophotometry and the HPLC method were established for determination of the DNR in the DNRSL. The release of DNR from DNRSL in HBS (pH 7.5) and rat serum at 37 oC were examined. The results showed that the DNRSL had high entrapment efficiency (>85%), small size and slow release.展开更多
Objective To elevate the immunological effect of subunit influenza vaccine in infants and aged people （over 60） using liposomal adjuvant in the context of its relatively low immunity and to investigate the relation ...Objective To elevate the immunological effect of subunit influenza vaccine in infants and aged people （over 60） using liposomal adjuvant in the context of its relatively low immunity and to investigate the relation between vaccine antigens and liposomal characteristics. Methods Several formulations of liposomal subunit influenza vaccine were prepared. Their relevant characteristics were investigated to optimize the preparation method. Antisera obtained from immunizinged mice were used to evaluate the antibody titers of various samples by HI and EL1SA. Results Liposomal trivalent influenza vaccine prepared by film evaporation in combinedation with freeze-drying significantly increased its immunological effect in SPF Balb/e mice. Liposomal vaccine stimulated the antibody titer of H3N2, H1N1, and B much stronger than conventional influenza vaccine. As a result, liposomal vaccine （mean size： 4.5-5.5 pm, entrapment efficiency： 30%-40%） significantly increased the immunological effect of subunit influenza vaccine. Conclusion The immune effect of liposomal vaccine depends on different antigens, and enhanced immunity is not positively correlated with the mean size of liposome or its entrapped efficiency.展开更多
Background:The primary aim of this research was to address the significant challenge of low and unpredictable drug absorption following oral administration,which often occurs due to poor water solubility.Niosomes,whic...Background:The primary aim of this research was to address the significant challenge of low and unpredictable drug absorption following oral administration,which often occurs due to poor water solubility.Niosomes,which are lipid-based vesicles,have been explored as potential solutions to increase the solubility of water-insoluble drugs.Methods:Niosomal suspensions were prepared with the thin-film hydration method.Various concentrations of Span 20,Span 40,Tween 60,and cholesterol were used to optimize the formulation.The resulting formulations were characterized,and their properties were assessed.Results:The optimal formulation,namely,NS6,which was composed of Span 40(200 mg)and cholesterol(40 mg),had a size of 206.1 nm and a zeta potential of-36.5 mV.Adjusting the surfactant concentration resulted in a maximum drug entrapment efficiency of 88.89%.Statistical analysis confirmed that NS6 was the optimal formulation.Conclusion:This study demonstrates that niosomal suspensions are promising drug delivery systems with potential for use in treating type 2 diabetes.Niosomes facilitate the sustained release and enhanced solubility of drugs,rendering them convenient and effective tools for enhancing drug delivery to target sites.展开更多
The objective of the present research was to prepare and characterize naproxen loaded niosomes by ether injection method.A total of sixteen formulations were prepared by ether injection method by varying the type and ...The objective of the present research was to prepare and characterize naproxen loaded niosomes by ether injection method.A total of sixteen formulations were prepared by ether injection method by varying the type and concentration of surfactant.All the formulations were evaluated for drug content,entrapment efficiency,loading capacity and drug release profiles.Based on evaluation parameters,formulation E14 prepared by ether injection method showed entrapment efficiency of 95.86%,drug content of 94.9%,zeta potential value of−31.9 mV,suggesting its higher stability and particle diameter of about 393.9 nm.In-vitro release studies also showed that of all the formulations,E14 released about 88.9%by the end of 12 hours,showing a sustained release pattern with high amount of drug release when compared to the other formulations.Drug release kinetic studies of optimized formulation(E14)followed zero order release with R2 value of 0.987 and showed super case 2 transport mechanism.Based on the results,tween 80 with 1:1 ratio of drug to surfactant was considered as the best formulation for the preparation of naproxen loaded niosomes by ether injection method.展开更多
The main objective of this study is to formulate etoricoxib niosomes as vesicular carriers for site specific drug delivery.Niosomes are novel vesicular carriers,in which the drug is incorporated in a vesicle.Niosomal ...The main objective of this study is to formulate etoricoxib niosomes as vesicular carriers for site specific drug delivery.Niosomes are novel vesicular carriers,in which the drug is incorporated in a vesicle.Niosomal vesicles are formed by hydrating mixture of cholesterol and nonionic surfactants.Niosomes can increase the permeability of the skin(stratum corneum and epidermis),by avoiding the first pass metabolism and also reduce the side effects.Etoricoxib is a potent new COX-2 inhibitor used in the treatment of osteoarthritis,rheumatoid arthritis,ankylosing spondylitis,acute gout arthritis etc.Two formulations were prepared by thin film hydration technique using the drug,cholesterol and surfactants Tween 80(F1)and Span 60(F2).Another two formulations were prepared by ether injection method using cholesterol and surfactants Tween 80(F3)and Span 60(F4).Each formulation was evaluated for drug content,entrapment efficiency,mean vesicular diameter,zeta potential and In-vitro drug release studies.Among the four formulations,F2 formulation containing the drug and Span 60 showed maximal drug content of 95.57%,entrapment efficiency of 96.40%,mean vesicular diameter of 463.7 nm,zeta potential of-80.5 mV,in-vitro drug release of 95.14%in 12 h,and the drug release followed the first order with non-fickian diffusion mechanism by thin film hydration technique.Hence,the thin film hydration technique is an optimized technique for the preparation of etoricoxib niosomes.展开更多
基金Natural Science Foundation of Gansu Provence (Grant No. 0803RJZA079)Foundation of Herb Medicin Research of Gansu Provence (Grant No. GZK-2009-1)
文摘A simple HPLC method was established for the determination of entrapment efficiency of a new 5-FPE liposome formulation. Chromatographic separation was performed on a Kromasil 100 A C18 column （350 mm×4.6 mm, 5 μm）. The mobile phase was consisted of methanol-water （58：42, v/v）. The flow rate of mobile phase was set at 0.8 mL/min. The UV detection wavelength was 271 nm, and the column temperature was 30 ℃. The linear range of 5-FPE was from 0.8-12.8 μg/mL, r = 0.9999. The RSD of intm-day and inter-day precision were less than 2.97%. The average recovery was from 96.8%-104.6% with RSD less than 2.24%. The method was simple, rapid, accurate, and sensitive. It is suitable for the determination of entrapment efficiency of the 5-FPE liposome formulation.
文摘This report studied on pharmaceutical characteristics of the stealth liposome containing dau-norubicin (DNR). The shape, size, entrapment efficiency and stability of the daunorubicin stealth liposomes (DNRSL) were examined. Visible spectrophotometry and the HPLC method were established for determination of the DNR in the DNRSL. The release of DNR from DNRSL in HBS (pH 7.5) and rat serum at 37 oC were examined. The results showed that the DNRSL had high entrapment efficiency (>85%), small size and slow release.
文摘Objective To elevate the immunological effect of subunit influenza vaccine in infants and aged people （over 60） using liposomal adjuvant in the context of its relatively low immunity and to investigate the relation between vaccine antigens and liposomal characteristics. Methods Several formulations of liposomal subunit influenza vaccine were prepared. Their relevant characteristics were investigated to optimize the preparation method. Antisera obtained from immunizinged mice were used to evaluate the antibody titers of various samples by HI and EL1SA. Results Liposomal trivalent influenza vaccine prepared by film evaporation in combinedation with freeze-drying significantly increased its immunological effect in SPF Balb/e mice. Liposomal vaccine stimulated the antibody titer of H3N2, H1N1, and B much stronger than conventional influenza vaccine. As a result, liposomal vaccine （mean size： 4.5-5.5 pm, entrapment efficiency： 30%-40%） significantly increased the immunological effect of subunit influenza vaccine. Conclusion The immune effect of liposomal vaccine depends on different antigens, and enhanced immunity is not positively correlated with the mean size of liposome or its entrapped efficiency.
文摘Background:The primary aim of this research was to address the significant challenge of low and unpredictable drug absorption following oral administration,which often occurs due to poor water solubility.Niosomes,which are lipid-based vesicles,have been explored as potential solutions to increase the solubility of water-insoluble drugs.Methods:Niosomal suspensions were prepared with the thin-film hydration method.Various concentrations of Span 20,Span 40,Tween 60,and cholesterol were used to optimize the formulation.The resulting formulations were characterized,and their properties were assessed.Results:The optimal formulation,namely,NS6,which was composed of Span 40(200 mg)and cholesterol(40 mg),had a size of 206.1 nm and a zeta potential of-36.5 mV.Adjusting the surfactant concentration resulted in a maximum drug entrapment efficiency of 88.89%.Statistical analysis confirmed that NS6 was the optimal formulation.Conclusion:This study demonstrates that niosomal suspensions are promising drug delivery systems with potential for use in treating type 2 diabetes.Niosomes facilitate the sustained release and enhanced solubility of drugs,rendering them convenient and effective tools for enhancing drug delivery to target sites.
文摘The objective of the present research was to prepare and characterize naproxen loaded niosomes by ether injection method.A total of sixteen formulations were prepared by ether injection method by varying the type and concentration of surfactant.All the formulations were evaluated for drug content,entrapment efficiency,loading capacity and drug release profiles.Based on evaluation parameters,formulation E14 prepared by ether injection method showed entrapment efficiency of 95.86%,drug content of 94.9%,zeta potential value of−31.9 mV,suggesting its higher stability and particle diameter of about 393.9 nm.In-vitro release studies also showed that of all the formulations,E14 released about 88.9%by the end of 12 hours,showing a sustained release pattern with high amount of drug release when compared to the other formulations.Drug release kinetic studies of optimized formulation(E14)followed zero order release with R2 value of 0.987 and showed super case 2 transport mechanism.Based on the results,tween 80 with 1:1 ratio of drug to surfactant was considered as the best formulation for the preparation of naproxen loaded niosomes by ether injection method.
文摘The main objective of this study is to formulate etoricoxib niosomes as vesicular carriers for site specific drug delivery.Niosomes are novel vesicular carriers,in which the drug is incorporated in a vesicle.Niosomal vesicles are formed by hydrating mixture of cholesterol and nonionic surfactants.Niosomes can increase the permeability of the skin(stratum corneum and epidermis),by avoiding the first pass metabolism and also reduce the side effects.Etoricoxib is a potent new COX-2 inhibitor used in the treatment of osteoarthritis,rheumatoid arthritis,ankylosing spondylitis,acute gout arthritis etc.Two formulations were prepared by thin film hydration technique using the drug,cholesterol and surfactants Tween 80(F1)and Span 60(F2).Another two formulations were prepared by ether injection method using cholesterol and surfactants Tween 80(F3)and Span 60(F4).Each formulation was evaluated for drug content,entrapment efficiency,mean vesicular diameter,zeta potential and In-vitro drug release studies.Among the four formulations,F2 formulation containing the drug and Span 60 showed maximal drug content of 95.57%,entrapment efficiency of 96.40%,mean vesicular diameter of 463.7 nm,zeta potential of-80.5 mV,in-vitro drug release of 95.14%in 12 h,and the drug release followed the first order with non-fickian diffusion mechanism by thin film hydration technique.Hence,the thin film hydration technique is an optimized technique for the preparation of etoricoxib niosomes.
