What is already known about this topic?Enterococcus spp.,while naturally occurring as commensal bacteria in the gastrointestinal tract of animals and humans,have emerged as significant opportunistic pathogens in healt...What is already known about this topic?Enterococcus spp.,while naturally occurring as commensal bacteria in the gastrointestinal tract of animals and humans,have emerged as significant opportunistic pathogens in healthcare settings.What is added by this report?A comprehensive surveillance study revealed enterococci in 14.39%of clinical samples from dogs and cats across China during 2018–2021.Multidrugresistant enterococcal infections showed significant correlation with urinary tract catheterization and extended hospitalization periods.Notably,pet-derived Enterococcus faecalis isolates demonstrated high genetic similarity with strains isolated from humans,farm animals,and environmental sources.What are the implications for public health practice?These findings underscore the critical need for enhanced surveillance of enterococcal infections and implementation of stringent aseptic protocols in veterinary clinical settings.Particular attention should be directed toward linezolid-resistant Enterococcus faecalis infections due to their demonstrated potential for transmission between pets and humans.展开更多
Mycotic aneurysms are a rare finding in the post-antibiotic era but must still be considered as a cause of abdominal pain in a patient with endocarditis. We present a case of a 47-year-old man with a history of IV dru...Mycotic aneurysms are a rare finding in the post-antibiotic era but must still be considered as a cause of abdominal pain in a patient with endocarditis. We present a case of a 47-year-old man with a history of IV drug use and a prior episode of endocarditis who developed a mycotic aneurysm while hospitalized on IV antibiotic therapy for aortic valve endocarditis. Due to their life-threatening character and often lack of evident clinical picture, mycotic aneurysms should still remain in high suspicion for a patient with abdominal pain in the setting of endocarditis.展开更多
Probiotics which can provide potential health benefits for consumers and prevent disease, is the most important research field for functional food in the future. It is the key point that how much the count of bacteria...Probiotics which can provide potential health benefits for consumers and prevent disease, is the most important research field for functional food in the future. It is the key point that how much the count of bacteria and how long it can preserve. Freeze-drying skill is one of the best preservation methods, but it＇s defect that damage to biological systems can be attributed to changes in the physical state of membrane lipids or/and changes in the structure of sensitive proteins and the decreased survival rate. This work used pretreatment methods-cold shock and hot shock to reduce the damage to bacteria during freeze-drying. Using unpretreated samples as control, aspects of cold shock or heat shock. The response of Enterococcous faecalis A31 was investigated during lag phase, the middle of exponential growth phase and the terminal of exponential growth phase. The results suggested that when cold shock, the shock protein concentration produced by treated sample within 2 h during the middle of exponential growth phase was higher than 4, 8, 24 h, The concentration of shock protein produced by treated sample at 10 ℃ was higher than 20℃, The concentration of sample protein at the middle of exponential growth phase was higher than that at the terminal of exponential growth phase. After one-month storage, the survival rate at 10℃ was better than 20 ℃ and 4 ℃comparatively. The survival rate at （6 h） 4 ℃/24 h （lag period） was higher than （10 h） 4 ℃/24 h （the middle of exponential growth phase）, and the effect of 10℃/8 h shock treatments was best during the middle of exponential growth phase, when hot shock, The concentration of heat shock proteins produced was not obvious, and survival rate was higher at 45 ℃, 30 min than at 45 ℃, 60 min.展开更多
基金Supported by the National Key Research and Development Program of China(2022YFD1800400)National Natural Science Foundation of China(81991531,32002339)2115 Talent Development Program of China Agricultural University.
文摘What is already known about this topic?Enterococcus spp.,while naturally occurring as commensal bacteria in the gastrointestinal tract of animals and humans,have emerged as significant opportunistic pathogens in healthcare settings.What is added by this report?A comprehensive surveillance study revealed enterococci in 14.39%of clinical samples from dogs and cats across China during 2018–2021.Multidrugresistant enterococcal infections showed significant correlation with urinary tract catheterization and extended hospitalization periods.Notably,pet-derived Enterococcus faecalis isolates demonstrated high genetic similarity with strains isolated from humans,farm animals,and environmental sources.What are the implications for public health practice?These findings underscore the critical need for enhanced surveillance of enterococcal infections and implementation of stringent aseptic protocols in veterinary clinical settings.Particular attention should be directed toward linezolid-resistant Enterococcus faecalis infections due to their demonstrated potential for transmission between pets and humans.
文摘Mycotic aneurysms are a rare finding in the post-antibiotic era but must still be considered as a cause of abdominal pain in a patient with endocarditis. We present a case of a 47-year-old man with a history of IV drug use and a prior episode of endocarditis who developed a mycotic aneurysm while hospitalized on IV antibiotic therapy for aortic valve endocarditis. Due to their life-threatening character and often lack of evident clinical picture, mycotic aneurysms should still remain in high suspicion for a patient with abdominal pain in the setting of endocarditis.
基金Supported by National Agricultural Translation Fund (02EFN212301073)
文摘Probiotics which can provide potential health benefits for consumers and prevent disease, is the most important research field for functional food in the future. It is the key point that how much the count of bacteria and how long it can preserve. Freeze-drying skill is one of the best preservation methods, but it＇s defect that damage to biological systems can be attributed to changes in the physical state of membrane lipids or/and changes in the structure of sensitive proteins and the decreased survival rate. This work used pretreatment methods-cold shock and hot shock to reduce the damage to bacteria during freeze-drying. Using unpretreated samples as control, aspects of cold shock or heat shock. The response of Enterococcous faecalis A31 was investigated during lag phase, the middle of exponential growth phase and the terminal of exponential growth phase. The results suggested that when cold shock, the shock protein concentration produced by treated sample within 2 h during the middle of exponential growth phase was higher than 4, 8, 24 h, The concentration of shock protein produced by treated sample at 10 ℃ was higher than 20℃, The concentration of sample protein at the middle of exponential growth phase was higher than that at the terminal of exponential growth phase. After one-month storage, the survival rate at 10℃ was better than 20 ℃ and 4 ℃comparatively. The survival rate at （6 h） 4 ℃/24 h （lag period） was higher than （10 h） 4 ℃/24 h （the middle of exponential growth phase）, and the effect of 10℃/8 h shock treatments was best during the middle of exponential growth phase, when hot shock, The concentration of heat shock proteins produced was not obvious, and survival rate was higher at 45 ℃, 30 min than at 45 ℃, 60 min.
