目的:采用高效液相色谱法测定化合物ent-kaur-16-en-19-oic ac id在光叶番荔枝不同部位和番荔枝属不同种类植物叶子中的含量。方法:色谱条件为:Nuc leosil C18色谱柱,流动相选用乙睛:水(含1%冰醋酸)=67.5:32.5,检测波长211nm。结果:平...目的:采用高效液相色谱法测定化合物ent-kaur-16-en-19-oic ac id在光叶番荔枝不同部位和番荔枝属不同种类植物叶子中的含量。方法:色谱条件为:Nuc leosil C18色谱柱,流动相选用乙睛:水(含1%冰醋酸)=67.5:32.5,检测波长211nm。结果:平均回收率为98.41%,光叶番荔枝树皮、光叶番荔枝树枝(去皮)、光叶番荔枝树叶、AP番荔枝树叶、牛心番荔枝树叶、山刺番荔枝树叶中化合物ent-kaur-16-en-19-oic ac id的含量分别为0.7147%、0.01252%、0.01773%、0.04212%、0.007305%、0.09083%。结论:在光叶番荔枝各部位中,树皮的含量最高,达0.7147%,在所测番荔枝属植物的树叶中,山刺番荔枝树叶中含量最高,达0.09083%。展开更多
目的:探讨Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid(5F)对人绒癌JAR细胞侵袭、转移、黏附的影响及MMP-9、VEGF表达的变化。方法:用不同浓度的5F作用JAR细胞,MTT比色法,Transwell小室,细胞黏附实验观察其增殖、侵袭、迁移、黏附...目的:探讨Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid(5F)对人绒癌JAR细胞侵袭、转移、黏附的影响及MMP-9、VEGF表达的变化。方法:用不同浓度的5F作用JAR细胞,MTT比色法,Transwell小室,细胞黏附实验观察其增殖、侵袭、迁移、黏附能力的变化;Western blot方法检测5F对JAR细胞MMP-9、VEGF表达水平的影响。结果:作用6 h,5F能抑制JAR细胞的增殖、转移、侵袭、黏附,其抑制率呈剂量效应(P<0.05)。5F作用24 h JAR细胞,MMP-9、VEGF蛋白表达水平能明显下调。结论:5F能抑制JAR细胞的侵袭、转移、黏附并下调MMP-9、VEGF蛋白表达水平。展开更多
A new diterpenoid dimer annonebinide A has been isolated from the stems of Annona glabra. Its structure was determined to be ent-16a-hydroxykauran-17-yl ent-16b-kauran-17-oate on the basis of spectroscopic and chemic...A new diterpenoid dimer annonebinide A has been isolated from the stems of Annona glabra. Its structure was determined to be ent-16a-hydroxykauran-17-yl ent-16b-kauran-17-oate on the basis of spectroscopic and chemical evidence.展开更多
A new ent-kaurane diterpene was isolated from the rhizome of Aralia fargesii. On the basis of chemical and spectral evidences, its structure was established as 16 alpha-hydroxy-17-isovaleroyloxy-ent-kauran-19-oic acid.
Two new diterpenoids,3,4,18β-cyclopropa-ent-abieta-8（14）,13（15）-dien-16,12-olide（1） and 3-oxojolkinolide B（2）,were isolated from the roots of Suregada glomerulata（Blume） Baill.Their structures were determin...Two new diterpenoids,3,4,18β-cyclopropa-ent-abieta-8（14）,13（15）-dien-16,12-olide（1） and 3-oxojolkinolide B（2）,were isolated from the roots of Suregada glomerulata（Blume） Baill.Their structures were determined by spectroscopic evidences. Compound 2 showed moderate cytotoxicity.展开更多
文摘目的:采用高效液相色谱法测定化合物ent-kaur-16-en-19-oic ac id在光叶番荔枝不同部位和番荔枝属不同种类植物叶子中的含量。方法:色谱条件为:Nuc leosil C18色谱柱,流动相选用乙睛:水(含1%冰醋酸)=67.5:32.5,检测波长211nm。结果:平均回收率为98.41%,光叶番荔枝树皮、光叶番荔枝树枝(去皮)、光叶番荔枝树叶、AP番荔枝树叶、牛心番荔枝树叶、山刺番荔枝树叶中化合物ent-kaur-16-en-19-oic ac id的含量分别为0.7147%、0.01252%、0.01773%、0.04212%、0.007305%、0.09083%。结论:在光叶番荔枝各部位中,树皮的含量最高,达0.7147%,在所测番荔枝属植物的树叶中,山刺番荔枝树叶中含量最高,达0.09083%。
文摘目的:探讨Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid(5F)对人绒癌JAR细胞侵袭、转移、黏附的影响及MMP-9、VEGF表达的变化。方法:用不同浓度的5F作用JAR细胞,MTT比色法,Transwell小室,细胞黏附实验观察其增殖、侵袭、迁移、黏附能力的变化;Western blot方法检测5F对JAR细胞MMP-9、VEGF表达水平的影响。结果:作用6 h,5F能抑制JAR细胞的增殖、转移、侵袭、黏附,其抑制率呈剂量效应(P<0.05)。5F作用24 h JAR细胞,MMP-9、VEGF蛋白表达水平能明显下调。结论:5F能抑制JAR细胞的侵袭、转移、黏附并下调MMP-9、VEGF蛋白表达水平。
文摘A new diterpenoid dimer annonebinide A has been isolated from the stems of Annona glabra. Its structure was determined to be ent-16a-hydroxykauran-17-yl ent-16b-kauran-17-oate on the basis of spectroscopic and chemical evidence.
文摘A new ent-kaurane diterpene was isolated from the rhizome of Aralia fargesii. On the basis of chemical and spectral evidences, its structure was established as 16 alpha-hydroxy-17-isovaleroyloxy-ent-kauran-19-oic acid.
基金the National Science and Technology Project of China(No.2009ZX09301-003-12-1) for financial support of this work
文摘Two new diterpenoids,3,4,18β-cyclopropa-ent-abieta-8（14）,13（15）-dien-16,12-olide（1） and 3-oxojolkinolide B（2）,were isolated from the roots of Suregada glomerulata（Blume） Baill.Their structures were determined by spectroscopic evidences. Compound 2 showed moderate cytotoxicity.
