Circulating tumor cells(CTCs) are tumor cells that enter the blood circulation after detaching from the primary tumor and can migrate to reach distant organs, where they can give rise to aggressive metastasis. Clinica...Circulating tumor cells(CTCs) are tumor cells that enter the blood circulation after detaching from the primary tumor and can migrate to reach distant organs, where they can give rise to aggressive metastasis. Clinical studies have revealed that the presence of CTCs in peripheral blood is correlated with disease progression in lung cancer. However, as CTCs are rare cancer cells released from tumors into the bloodstream, both enrichment and sensitive detection methods are technically challenging. In order to best understand how CTCs are currently being deployed, this review mainly focuses on the different detection methods for CTCs. Furthermore, we will describe the clinical impact of circulating tumor cells in lung cancer and discuss their potential use as biomarker to guide the prognosis.展开更多
A personalized trustworthy service selection method is proposed to fully express the features of trust, emphasize the importance of user preference and improve the trustworthiness of service selection. The trustworthi...A personalized trustworthy service selection method is proposed to fully express the features of trust, emphasize the importance of user preference and improve the trustworthiness of service selection. The trustworthiness of web service is defined as customized multi-dimensional trust metrics and the user preference is embodied in the weight of each trust metric. A service selection method combining AHP （analytic hierarchy process） and PROMETHEE （preference ranking organization method for enrichment evaluations） is proposed. AHP is used to determine the weights of trust metrics according to users＇ preferences. Hierarchy and pairwise comparison matrices are constructed. The weights of trust metrics are derived from the highest eigenvalue and eigenvector of the matrix. to obtain the final rank of candidate services. The preference functions are defined according to the inherent characteristics of the trust metrics and net outranking flows are calculated. Experimental results show that the proposed method can effectively express users＇ personalized preferences for trust metrics, and the trustworthiness of service ranking and selection is efficiently improved.展开更多
In recent years,genome editing with site-specific nucleases,such as ZFN（zinc finger nuclease）,TALEN（transcription activatorlike effector nucleases）,and CRISPR/Cas9（the type II bacterial clustered,regularly inters...In recent years,genome editing with site-specific nucleases,such as ZFN（zinc finger nuclease）,TALEN（transcription activatorlike effector nucleases）,and CRISPR/Cas9（the type II bacterial clustered,regularly interspaced,short palindromic repeats-associated protein 9）,has gained popularity for use in cell lines,animals,and plants（Urnov et al.,2010;Miller et al.,2011;Cong et al.展开更多
A sample enrichment method focusing on the minor targeted components was established to help them to be successfully separated by pH-zone refining CCC.Seven minor indole alkaloids in Uncaria rhynchophylla(Miq.)Miq.ex ...A sample enrichment method focusing on the minor targeted components was established to help them to be successfully separated by pH-zone refining CCC.Seven minor indole alkaloids in Uncaria rhynchophylla(Miq.)Miq.ex Havil(UR)were chosen to show the advantage of this method.The sample enrichment and separation were展开更多
We develop a locally mass-conservative enriched Petrov-Galerkin(EPG)method without any penalty term for the simulation of Darcy flow in fractured porous media.The discrete fracture model is applied to model the fractu...We develop a locally mass-conservative enriched Petrov-Galerkin(EPG)method without any penalty term for the simulation of Darcy flow in fractured porous media.The discrete fracture model is applied to model the fractures as the lower dimensional fracture interfaces.The new method enriches the approximation trial space of the conforming continuous Galerkin(CG)method with bubble functions and enriches the approximation test space of the CG method with piecewise constant functions in the fractures and the surrounding porous media.We propose a framework for constructing the bubble functions and consider a decoupled algorithm for the EPG method.The solution of the pressure can be decoupled into two steps with a standard CG method and a post-processing correction.The post-processing correction based on the bubble functions in the matrix and the fractures can be solved separately,which is useful for parallel computing.We derive a priori and a posteriori error estimates for the problem.Numerical examples are presented to illustrate the performance of the proposed method.展开更多
Herein,a two-node beam element enriched based on the Lagrange and Hermite interpolation function is proposed to solve the governing equation of a functionally graded porous(FGP)curved nanobeam on an elastic foundation...Herein,a two-node beam element enriched based on the Lagrange and Hermite interpolation function is proposed to solve the governing equation of a functionally graded porous(FGP)curved nanobeam on an elastic foundation in a hygro–thermo–magnetic environment.The material properties of curved nanobeams change continuously along the thickness via a power-law distribution,and the porosity distributions are described by an uneven porosity distribution.The effects of magnetic fields,temperature,and moisture on the curved nanobeam are assumed to result in axial loads and not affect the mechanical properties of the material.The equilibrium equations of the curved nanobeam are derived using Hamilton’s principle based on various beam theories,including the classical theory,first-order shear deformation theory,and higher-order shear deformation theory,and the nonlocal elasticity theory.The accuracy of the proposed method is verified by comparing the results obtained with those of previous reliable studies.Additionally,the effects of different parameters on the free vibration behavior of the FGP curved nanobeams are investigated comprehensively.展开更多
基金Supported by grants from the Natural Science Foundation of Heilongjiang Province(No.H201335)Development Center for Medical Science and Technology,Ministry of Health,China(No.W2012FZ105)
文摘Circulating tumor cells(CTCs) are tumor cells that enter the blood circulation after detaching from the primary tumor and can migrate to reach distant organs, where they can give rise to aggressive metastasis. Clinical studies have revealed that the presence of CTCs in peripheral blood is correlated with disease progression in lung cancer. However, as CTCs are rare cancer cells released from tumors into the bloodstream, both enrichment and sensitive detection methods are technically challenging. In order to best understand how CTCs are currently being deployed, this review mainly focuses on the different detection methods for CTCs. Furthermore, we will describe the clinical impact of circulating tumor cells in lung cancer and discuss their potential use as biomarker to guide the prognosis.
基金The National Natural Science Foundation of China(No.60973149)the Open Funds of State Key Laboratory of Computer Science of the Chinese Academy of Sciences(No.SYSKF1110)+1 种基金the Doctoral Fund of Ministry of Education of China(No.20100092110022)the College Industrialization Project of Jiangsu Province(No.JHB2011-3)
文摘A personalized trustworthy service selection method is proposed to fully express the features of trust, emphasize the importance of user preference and improve the trustworthiness of service selection. The trustworthiness of web service is defined as customized multi-dimensional trust metrics and the user preference is embodied in the weight of each trust metric. A service selection method combining AHP （analytic hierarchy process） and PROMETHEE （preference ranking organization method for enrichment evaluations） is proposed. AHP is used to determine the weights of trust metrics according to users＇ preferences. Hierarchy and pairwise comparison matrices are constructed. The weights of trust metrics are derived from the highest eigenvalue and eigenvector of the matrix. to obtain the final rank of candidate services. The preference functions are defined according to the inherent characteristics of the trust metrics and net outranking flows are calculated. Experimental results show that the proposed method can effectively express users＇ personalized preferences for trust metrics, and the trustworthiness of service ranking and selection is efficiently improved.
基金supported by the Science Research Center of the National Research Foundation of Korea (No. 2015R1A5A1009701).
文摘In recent years,genome editing with site-specific nucleases,such as ZFN（zinc finger nuclease）,TALEN（transcription activatorlike effector nucleases）,and CRISPR/Cas9（the type II bacterial clustered,regularly interspaced,short palindromic repeats-associated protein 9）,has gained popularity for use in cell lines,animals,and plants（Urnov et al.,2010;Miller et al.,2011;Cong et al.
基金supported by the National Science and Technology Major Project for Major Drug Development(No.2013ZX09508104)the Traditional Chinese Medicine Industry Research Special Project(No.201307002)the National Science&Technology Major Project Key New Drug Creation and Manufacturing program(No.2011ZX09307002-03)of the People's Republic of China
文摘A sample enrichment method focusing on the minor targeted components was established to help them to be successfully separated by pH-zone refining CCC.Seven minor indole alkaloids in Uncaria rhynchophylla(Miq.)Miq.ex Havil(UR)were chosen to show the advantage of this method.The sample enrichment and separation were
基金supported by the National Key Research and Development Project of China(Grant No.2023YFA1011702)the NSF of China(Grant No.12122115)。
文摘We develop a locally mass-conservative enriched Petrov-Galerkin(EPG)method without any penalty term for the simulation of Darcy flow in fractured porous media.The discrete fracture model is applied to model the fractures as the lower dimensional fracture interfaces.The new method enriches the approximation trial space of the conforming continuous Galerkin(CG)method with bubble functions and enriches the approximation test space of the CG method with piecewise constant functions in the fractures and the surrounding porous media.We propose a framework for constructing the bubble functions and consider a decoupled algorithm for the EPG method.The solution of the pressure can be decoupled into two steps with a standard CG method and a post-processing correction.The post-processing correction based on the bubble functions in the matrix and the fractures can be solved separately,which is useful for parallel computing.We derive a priori and a posteriori error estimates for the problem.Numerical examples are presented to illustrate the performance of the proposed method.
基金supported by Bualuang ASEAN Chair Professor Fund.
文摘Herein,a two-node beam element enriched based on the Lagrange and Hermite interpolation function is proposed to solve the governing equation of a functionally graded porous(FGP)curved nanobeam on an elastic foundation in a hygro–thermo–magnetic environment.The material properties of curved nanobeams change continuously along the thickness via a power-law distribution,and the porosity distributions are described by an uneven porosity distribution.The effects of magnetic fields,temperature,and moisture on the curved nanobeam are assumed to result in axial loads and not affect the mechanical properties of the material.The equilibrium equations of the curved nanobeam are derived using Hamilton’s principle based on various beam theories,including the classical theory,first-order shear deformation theory,and higher-order shear deformation theory,and the nonlocal elasticity theory.The accuracy of the proposed method is verified by comparing the results obtained with those of previous reliable studies.Additionally,the effects of different parameters on the free vibration behavior of the FGP curved nanobeams are investigated comprehensively.
