The quest for enrichment model of continental shale oil in the Zhanhua Sag of the JiyangDepression in the Bohai Bay Basin to provide reference for exploration and development requires acomprehensive approach. Therefor...The quest for enrichment model of continental shale oil in the Zhanhua Sag of the JiyangDepression in the Bohai Bay Basin to provide reference for exploration and development requires acomprehensive approach. Therefore, this study employs rock pyrolysis, Scanning Electron Microscopy(SEM), X-Ray diffraction analysis (XRD), Nuclear Magnetic Resonance (NMR), and other experiments toanalyze the conditions for shale oil enrichment and establish its patterns. The results show that favorablehydrocarbon generation potential and appropriate thermal maturation degree control “in situ enrichment”;while the storage capacity and the mobility of shale oil determine “migration enrichment.” In the process,the TOC governs the oil-generating capacity of shale with medium to large pores and microfractures servingas the main enrichment spaces and migration pathways for shale oil. Based on the deposition model, thestudy area can be divided into five lithofacies stages (I-algal limestone, II-laminated marl, III-laminatedrecrystallized limestone, IV-laminated mudstones, and IV-blocky calcareous mudstones). Integrating thegeochemical parameters into the sedimentary patterns makes it clear that the study area underwent two phasesof hydrocarbon expulsion during the thermal evolution of source rocks (Stage II: 3 060–3 120 m and StageIV: 3 020–3 040 m). However, judging by the observed TOC (2% to 5.6%), thermal maturity (Ro>0.8%),S1 (>2 mg/g) and OSI (>100 mg/g) as well as moderate basin size, climate, and quantity of terrestrial input,the blocky calcareous mudstones (Stage IV) have better oil-prone characteristics and potential to generate asubstantial quantity of hydrocarbons at this stage. More so, with a brittleness index exceeding 60%, it exhibitsfavorable fracturability accounting for the main controlling factors and enrichment patterns of shale oil in thearea. Hence, this study further enriches and develops the theoretical understanding of shale oil enrichment inthe area, provides valuable insights for future exploration of continental shale oil in eastern China and othersimilar basin around the world.展开更多
Biomedical big data,characterized by its massive scale,multi-dimensionality,and heterogeneity,offers novel perspectives for disease research,elucidates biological principles,and simultaneously prompts changes in relat...Biomedical big data,characterized by its massive scale,multi-dimensionality,and heterogeneity,offers novel perspectives for disease research,elucidates biological principles,and simultaneously prompts changes in related research methodologies.Biomedical ontology,as a shared formal conceptual system,not only offers standardized terms for multi-source biomedical data but also provides a solid data foundation and framework for biomedical research.In this review,we summarize enrichment analysis and deep learning for biomedical ontology based on its structure and semantic annotation properties,highlighting how technological advancements are enabling the more comprehensive use of ontology information.Enrichment analysis represents an important application of ontology to elucidate the potential biological significance for a particular molecular list.Deep learning,on the other hand,represents an increasingly powerful analytical tool that can be more widely combined with ontology for analysis and prediction.With the continuous evolution of big data technologies,the integration of these technologies with biomedical ontologies is opening up exciting new possibilities for advancing biomedical research.展开更多
In addition to the organic matter type,abundance,thermal maturity,and shale reservoir space,the preservation conditions of source rocks play a key factor in affecting the quantity and quality of retained hydrocarbons ...In addition to the organic matter type,abundance,thermal maturity,and shale reservoir space,the preservation conditions of source rocks play a key factor in affecting the quantity and quality of retained hydrocarbons in source rocks of lacustrine shale,yet this aspect has received little attention.This paper,based on the case analysis,explores how preservation conditions influence the enrichment of mobile hydrocarbons in shale oil.Research showns that good preservation conditions play three key roles.(1)Ensure the retention of sufficient light hydrocarbons(C_(1)–C_(13)),medium hydrocarbons(C_(14)–C_(25))and small molecular aromatics(including 1–2 benzene rings)in the formation,which enhances the fluidity and flow of shale oil;(2)Maintain a high energy field(abnormally high pressure),thus facilitating the maximum outflow of shale oil;(3)Ensure that the retained hydrocarbons have the miscible flow condition of multi-component hydrocarbons(light hydrocarbons,medium hydrocarbons,heavy hydrocarbons,and heteroatomic compounds),so that the heavy hydrocarbons(C_(25+))and heavy components(non-hydrocarbons and asphaltenes)have improved fluidity and maximum flow capacity.In conclusion,in addition to the advantages of organic matter type,abundance,thermal maturity,and reservoir space,good preservation conditions of shale layers are essential for the formation of economically viable shale oil reservoirs,which should be incorporated into the evaluation criteria of shale oil-rich areas/segments and considered a necessary factor when selecting favorable exploration targets.展开更多
Anaerobic ammonia oxidation(Anammox)is an economical and sustainablewastewater nitrogen removal technology,and its application in the mainstream process is the inevitable trend of the development of Anammox.However,ho...Anaerobic ammonia oxidation(Anammox)is an economical and sustainablewastewater nitrogen removal technology,and its application in the mainstream process is the inevitable trend of the development of Anammox.However,how to effectively enriching Anammox bacteria from the activated sludge remains challenging and restricts its extensive applications.In this study,the rapid and efficient enrichment of Anammox bacteriawas achieved by raising the reflux ratio and nitrogen loading rate(NLR)using conventional activated sludge as the inoculant.In the screening phase(days 1–90),the reflux ratio was increased to discharge partial floc sludge,resulting in the relative abundance of Candidatus Brocadiaceae increased from0.04%to 22.54%,which effectively reduced thematrix and spatial competition between other microorganisms and Anammox bacteria.On day 90,the stoichiometric ratio of the Anammox process closely approached the theoretical value of 1:1.32:0.26,indicating that the Anammox reaction was the primary nitrogen removal process in the system.In the enrichment phase(days 91–238),the NLR increased from 0.43 to 1.20 kgN/(m^(3)·d)and removal efficiency was 71.89%,resulting in the relative abundance of Candidatus Brocadiaceae increased to 61.27%on day 180.The reactor operated steadily from days 444 to 498,maintaining the nitrogen removal rate(NRR)of 3.00 kgN/(m^(3)·d)and achieving successful sludge granulation with the particle size of 392.4μm.In short,this study provided a simple and efficient approach for enriching Anammox bacteria from the activated sludge,supporting to start an Anammox process efficiently.展开更多
Based on the basic data of drilling,logging,testing and geological experiments,the geological characteristics of the Permian Dalong Formation marine shales in the northern Sichuan Basin and the factors controlling sha...Based on the basic data of drilling,logging,testing and geological experiments,the geological characteristics of the Permian Dalong Formation marine shales in the northern Sichuan Basin and the factors controlling shale gas enrichment and high yield are studied.The results are obtained in four aspects.First,the high-quality shale of the Dalong Formation was formed after the deposition of the Permian Wujiaping Formation,and it is developed in the Kaijiang-Liangping trough in the northern part of Sichuan Basin,where deep-water continental shelf facies and deep-water reduction environment with thriving siliceous organisms have formed the black siliceous shale rich in organic matter.Second,the Dalong Formation shale contains both organic and inorganic pores,with stratification of alternated brittle and plastic minerals.In addition to organic pores,a large number of inorganic pores are developed even in ultra-deep(deeper than 4500 m)layers,contributing a total porosity of more than 5%,which significantly expands the storage space for shale gas.Third,the limestone at the roof and floor of the Dalong Formation acted as seal rock in the early burial and hydrocarbon generation stage,providing favorable conditions for the continuous hydrocarbon generation and rich gas preservation in shale interval.In the later reservoir stimulation process,it was beneficial to the lateral extension of the fractures,so as to achieve the optimal stimulation performance and increase the well-controlled resources.Combining the geological,engineering and economic conditions,the favorable area with depth less than 5500 m is determined to be 1800 km2,with resources of 5400×10^(8) m^(3).Fourth,the shale reservoirs of the Dalong Formation are thin but rich in shale gas.The syncline zone far away from the main faults in the high and steep tectonic zone,eastern Sichuan Basin,with depth less than 5500 m,is the most favorable target for producing the Permian shale gas under the current engineering and technical conditions.It mainly includes the Nanya syncline,Tanmuchang syncline and Liangping syncline.展开更多
Tropical coastal ecosystems,vital habitats for economically significant marine species,face increasing threats from eutrophication driven by human-induced nutrient pollution.This study investigated the metabolomic pro...Tropical coastal ecosystems,vital habitats for economically significant marine species,face increasing threats from eutrophication driven by human-induced nutrient pollution.This study investigated the metabolomic profile of Anadara granosa,a marine bivalve,to identify metabolites indicative of eutrophic conditions.Conducted in Cirebon Bay,Indonesia,the research spanned five locations reflecting varying eutrophication levels.Bottom-layer water samples were analyzed for pH,dissolved oxygen,salinity,water transparency,and nutrient concentrations(phosphate,nitrate,ammonia).Proton-Nuclear Magnetic Resonance(Proton-NMR)metabolomics of A.granosa revealed metabolite variations linked to water quality.Eutrophic conditions,prevalent in the bay’s northern side,were characterized by inorganic nitrogen levels above 0.2 mg L^(-1) and phosphate exceeding 0.024 mg L^(-1).These were linked to urban,aquaculture,and agricultural runoff,driving increased phytoplankton biomass and reduced salinity variability.Metabolomic analysis identified inosine and dimethylglycine as potential biomarkers.Elevated inosine levels in eutrophic environments suggest increased cellular activity due to greater food availability,while higher dimethylglycine concentrations detected in low-nutrient as its role in osmotic regulation.Both metabolites correlated with ammonia concentrations(P<0.05,R^(2)=0.631),a key contributor to eutrophication.These findings highlight inosine and dimethylglycine as effective indicators of nutrient enrichment,providing insights into eutrophication’s impact on coastal ecosystems and A.granosa’s physiological responses.展开更多
Macroalgae dominate nutrient dynamics and function as high-value foods for microbial,meio-and macrofaunal communities in coastal ecosystems.Because of this vital role,it is important to clarify the physiological infor...Macroalgae dominate nutrient dynamics and function as high-value foods for microbial,meio-and macrofaunal communities in coastal ecosystems.Because of this vital role,it is important to clarify the physiological information associated with environmental changes as it reflects their growth potential.To evaluate the effects of the changes in salinity and nutrients,the photosynthetic efficiency of a green macroalga Ulva fasciata from the Daya Bay was tested at a range of salinity(i.e.,31 to 10 psu)and nitrogen content(i.e.,5 to 60μmol L^(-1)).The results showed that cellular chlorophyll a(Chl a),carbohydrate and protein contents of U.fasciata were increased due to reduced salinity,and were decreased by interactive nitrogen enrichment.Within a short culture period(i.e.,18 h),the reduced salinity decreased the maximum photosynthetic efficiency(rETRmax and Pmax)derived from the rapid light response curve and photosynthetic oxygen evolution rate versus irradiance curve,respectively,as well as the saturation irradiance(E_(K)).This reducing effect diminished with enlonged cultivation time and reversed to a stimulating effect after 24 h of cultivation.The nitrogen enrichment stimulated the rETRmax and Pmax,as well as the E_(K),regardless of salinity,especially within short-term cultivation period(i.e.,<24 h).In addition,our results indicate that seawater freshening lowers the photosynthetic efficiency of U.fasciata in the short term,which is mitigated by nitrogen enrichment,but stimulates it in the long term,providing insight into how macroalgae thrive in coastal or estuarine waters where salinity and nutrients normally covary strongly.展开更多
Recent studies have highlighted the presence of lithium enrichment in coals within the Ordos Basin,which has garnered significant attention due to the potential economic value.However,most research has concentrated pr...Recent studies have highlighted the presence of lithium enrichment in coals within the Ordos Basin,which has garnered significant attention due to the potential economic value.However,most research has concentrated primarily on the coal seams of the Taiyuan and Shanxi formations,with limited reports on the Benxi Formation.To address this gap,our study focused on elucidating the geochemical characteristics and origins of the lithium enrichment in the No.8 coal of the Benxi Formation through the use of optical microscopy and inductively coupled plasma mass spectrometry(ICP-MS).The results showed that the No.8 coal was a bituminous coal,characterized by medium ash yield,low volatile matter,high total sulfur content and medium vitrinite proportion.The No.8 coal was enriched in Li(average 91.7 ppm,CC=6.55)and Zr(average 191 ppm,CC=5.30),the unusual enrichment of Li being primarily located in the middle of the coal.The minerals in the coal were predominantly clay minerals,along with minor amounts of pyrite,quartz and calcite.The occurrence mode of Li in the No.8 coal was associated with aluminosilicate minerals,presumably kaolinite.Based on geochemical characteristics,the sedimentary source of the No.8 coal was intermediate-felsic volcanic rock from the Yinshan oldland.The enrichment of Li can be attributed to the supply of terrestrial debris from the Yinshan oldland and the depositional environment.Our analysis identifies three distinct stages of lithium enrichment,emphasizing the critical role played by the terrestrial debris,as well as the acidic to partially reducing conditions,in facilitating this process.In conclusion,our study sheds light on the mechanisms underlying lithium enrichment in the No.8 coal of the Benxi Formation,highlighting the significance of geological factors in shaping the distribution and concentration of critical metals in coal.展开更多
Ultrasonic humidifiers are commonly used in households to maintain indoor humidity and generate a large number of droplets or spray aerosols.However,there have been various health concerns associated with humidifier u...Ultrasonic humidifiers are commonly used in households to maintain indoor humidity and generate a large number of droplets or spray aerosols.However,there have been various health concerns associated with humidifier use,largely due to aerosols generated during operation.Here,we investigated the size distribution,chemical composition,and charged fraction of aerosol particles emitted from commercial ultrasonic humidifiers.Heavy metals in water used for humidifiers were found to be highly enriched in the ultrasonic humidifier aerosols(UHA),with the enrichment factors ranging from 102 to 107.This enrichment may pose health concerns for the building occupants,as UHA concentrations of up to 106 particles/cm^(3) or 3 mg/m^(3) were observed.Furthermore,approximately 90%of UHA were observed to be electrically charged,for the first time according to our knowledge.Based on this discovery,we proposed and tested a newmethod to remove UHA by using a simple electrical field.The designed electrical field in this work can efficiently remove 81.4%of UHA.Therefore,applying this electrical field could be an effective method to significantly reduce the health risks by UHA.展开更多
The efficacy of DNA sequencing,particularly long reads nanopore sequencing,is critically dependent on the amount and quality of the input DNA.However,extracting high concentrations of DNA from low biomass samples,espe...The efficacy of DNA sequencing,particularly long reads nanopore sequencing,is critically dependent on the amount and quality of the input DNA.However,extracting high concentrations of DNA from low biomass samples,especially from solid matrices,presents significant challenges,this limitation not only substantially hampers the scope of environmental microbiology studies but also makes enhancing DNA yield indispensable in many instances.Therefore,in this study,we systematically evaluated the impact of four different DNA enrichment methods on both amplicon and metagenomic community analyses of solid-phase,low-biomass samples:permafrost soil and biofilm of sand filter.These methods include multiple displacement amplification(MDA),centrifugal filtration(CF),freeze vacuum drying at(FVD)as well as vacuum centrifugal at 35,45,and 60°C(namely VC35,VC45,VC60).Our results indicate that FVD was the most effective for increasing DNA concentration,while VC methods best preserved DNA fragment length.In contrast,the widely used MDA and CF methods exhibited biases,preferentially enriching low-GC content sequences,which affected both assembly and annotation outcomes.Metagenomic assembly from MDA and CF samples was suboptimal,with fewer contigs and no middle quality MAGs recovered compared to other methods.Community composition analysis revealed significant shifts across all enrichment methods,with Sphingomonas and Sphingorhabdus genera could be obviously enriched.These findings highlight the necessity and importance of carefully selecting DNA enrichment methods to ensure reliable metagenomic investigation of low-biomass environmental samples.展开更多
The geogenic enrichment of arsenic(As)extensively occurred in the riverine systems from the Qinghai-Tibetan Plateau under active geothermal discharge and chemical weathering conditions,while little is known about how ...The geogenic enrichment of arsenic(As)extensively occurred in the riverine systems from the Qinghai-Tibetan Plateau under active geothermal discharge and chemical weathering conditions,while little is known about how dissolved organic matter(DOM)transformation regulates the aquatic As concentrations.The present study revealed that the DOM components from the Singe Tsangpo River(STR)basin primarily consisted of protein-like components(81.30%±6.48%),with the microbially-endogenous production being a predominant source under the control of temperature and glacier-runoff recharge along the river flow path.Notably,the chemical weathering processes have significantly facilitated the enhancement of humic-like components in the river water.Besides,the groundwater DOM characteristics were predominantly influenced by the mobilization of sedimentary organic matter and the introduction of allochthonous DOM resulting from surface-water recharge.Interestingly,humic-like components facilitated As enrichment through complexation and competitive adsorption effects in both surface water and groundwater under oxidizing conditions,whichwas supported by the significant positive correlations between As and humiclike component(R^(2)=0.31/0.65,P<0.05/0.01)and the concurrent mobilization of As and humic-like components from sediment incubation experiments.Moreover,the Structural Equation Modeling analysis revealed a stronger contribution of humic-like components to the As enrichment in the groundwater compared with surface water,possibly due to the relatively strongermicrobial activity and enhanced mobilization of humic-like components in alluvial aquifers.The present study thus provided new insights into the transformation of DOM and its important role in facilitating As enrichment in the aquatic environment from alpine river basins.展开更多
Compared to discrete continental marginal basins,the mechanisms of hydrocarbon migration and enrichment in transform continental marginal basins are poorly understood.In this study,we conducted a comprehensive analysi...Compared to discrete continental marginal basins,the mechanisms of hydrocarbon migration and enrichment in transform continental marginal basins are poorly understood.In this study,we conducted a comprehensive analysis of the main source rocks,reservoirs,and vertical migration pathways within the Rovuma(RB)and Tanzania(TB)basins in East Africa utilizing drilling,logging,seismic,and geochemical data.The results indicate that the enhanced preservation conditions of the Lower Jurassic source rocks in the southwest could lead to the discovery of large natural gas fields in the southern TB and RB.The primary reservoir is a deep-water turbidite sandstone.Due to topographic differences,the expanse of the turbidite sandstones in the RB is significantly larger than those in Tanzania.The main vertical migration pathways are the western boundary fault zone of the Kerimbas Graben(WBFZ)and the Seagap fault zone(SFZ).In the RB,natural gas migrates vertically along the WBFZ and preferentially accumulates in the deep-water turbidite sandstones of the footwall under the control of the fluid potential.Conversely,in the southern TB,the deep natural gas first migrates upward along the SFZ,then moves along the shallow branch faults in the sandstones on both sides of the SFZ.展开更多
The Jinchuan magmatic Ni-Cu-PGE deposit is the largest single Ni sulfide deposit in the world.It consists primarily of orebody-24 in segment I,and orebody-1 and orebody-2 in segment II.The contents of platinum-group e...The Jinchuan magmatic Ni-Cu-PGE deposit is the largest single Ni sulfide deposit in the world.It consists primarily of orebody-24 in segment I,and orebody-1 and orebody-2 in segment II.The contents of platinum-group elements(PGE)in these orebodies decrease significantly from west to east across the deposit.However,the PGE characteristics of platinum-group minerals(PGM)and alloys,as well as their roles during mineralization in different orebodies,remain unclear.In this study,PGM and alloy occurrences in orebody-24 and orebody-2 had been observed by scanning electron microscopy(SEM)and spherical-aberration corrected scanning transmission electron microscope(Cs-STEM).The PGE contents were analyzed by SEM‒EDS for submicron-scale PGM and alloys.The results show that olivine in lherzolite mainly hosts PGM of PtTe and PdBi2,whereas pyroxene contains small amounts of PdBiTe.Pyrrhotite predominantly hosts(Ir-Rh-Pt)AsS,Pd(BiTe),and other PGM,as well as PtSn and PtOs alloys.Pentlandite mainly encloses Pd(BiTe),PdBi/PdBi2 and other PGM,as well as PtSn and PtFe alloys.Chalcopyrite primarily encloses PdBi/PdBi2,and other PGM,along with PtSn and IrOs.Distinct distribution patterns of PGE in PGM and alloys had been observed between different orebodies.Orebody-24 contains more(Ir-Rh-Pt)AsS minerals and PtFe/PtSn alloy grains,whereas orebody-2 has a higher proportion of Pt-and Pd-bearing PGM.The presence of euhedral alloys in silicate minerals from orebody-24 suggests that its parent magma had a higher PGE content before sulfide saturation than that of orebody-2.More than 90%of PGM and alloys in both orebody-24 and orebody-2 contain Pt and Pd,emphasizing their contributions to the elevated Pt and Pd concentrations.The different PGE distributions of PGM and alloys in the two orebodies suggest that thermodynamic conditions(fO2 and fS2)and semimetals,especially As,play critical roles in controlling PGE behavior and occurrence.展开更多
The relationship between marine transgression and the distribution of lacustrine organic matter has restricted shale oil and gas exploration for decades.In this study,the research objective is to analyze the sedimenta...The relationship between marine transgression and the distribution of lacustrine organic matter has restricted shale oil and gas exploration for decades.In this study,the research objective is to analyze the sedimentary environment and evaluate its influence on organic matter in transgressive lacustrine shale.The study uses various analyses including total organic carbon(TOC),Rock-Eval pyrolysis,gas chromatography-mass spectrometry(GC-MS),trace element and isotope analysis.Finally,the study proposes an enrichment model for organic matter.The lacustrine shale of the second member of the Funing Formation(E_(1)f^(2))is divided into three sequences.The results indicate that the depositional environment of the organic matter during this period was an arid and humid,reduced,closed,rift lake basin.In the first sequence,high salinity resulted from increased evaporation,leading to low primary biological productivity.At this time,the lake basin belonged to a salinized closed lake basin.Intermittent transgressions began in the second sequence,with the deep lake area still being dominated by a reducing environment.The third sequence saw the environment evolve into a closed lake basin characterized by a warm and humid freshwater environment with high primary productivity.Marine transgressions introduce a substantial amount of marine plankton,nutrient elements,as well as more CO_(2) and CO_(3)^(2−)into the lake,leading to increased primary productivity.The sedimentary model for transgressive lacustrine source rocks proposed here serves as an example for similar transgressive lake basins.展开更多
Receptor tyrosine kinases(RTKs)are biological enzymes expressed on cell membranes that can influence cellular signaling,and their overexpression in tumor cells makes them a key route to assess relevant tumor processes...Receptor tyrosine kinases(RTKs)are biological enzymes expressed on cell membranes that can influence cellular signaling,and their overexpression in tumor cells makes them a key route to assess relevant tumor processes.The development of a delivery system that targets and accumulates in RTKs overexpressing-cells at the on-target site is significant for the monitoring of tumor progression and clinical applications through longer tumor site signaling response under low injection frequency.Here,a host-vip nanoscale fluorescent probe SNI@ZIF-8 based on zeolitic imidazolate framework-8(ZIF-8)and a fluorescent probe SNI constructed from receptor tyrosine kinase inhibitor was proposed and prepared for targeting RTKs and enabling prolonged fluorescence imaging in vivo.The folded conformation of the probe SNI resulted in low background fluorescence,and the unfolding of the SNI conformation upon insertion of the RTKs active pocket showed significant fluorescence enhancement thus enabling real-time detection of RTKs.The host-vip system SNI@ZIF-8 could release vip molecules due to the presence of the enzyme,emphasizing the reporting of stable fluorescent signals over time under low injection frequency.SNI@ZIF-8 could provide a signal response on the cell membrane of RTKs overexpressing cells without interference from other substances,and provided a longer fluorescent signal than SNI at equivalent number of injections in tumor-bearing mice.The host-vip system SNI@ZIF-8,with its obvious tumor site enrichment ability and clear fluorescence imaging ability,could be successfully applied to the detection of RTKs on cell membranes in biological systems,providing a new strategy for determining the process of tumor development in clinical applications.展开更多
Taking the GY8HC well in the Gulong Sag of the Songliao Basin,NE China,as an example,this study utilized high-precision zircon U-Pb ages from volcanic ashes and AstroBayes method to estimate sedimentation rates.Throug...Taking the GY8HC well in the Gulong Sag of the Songliao Basin,NE China,as an example,this study utilized high-precision zircon U-Pb ages from volcanic ashes and AstroBayes method to estimate sedimentation rates.Through spectral analysis of high-resolution total organic carbon content(TOC),laboratory-measured free hydrocarbons(S_(1)),hydrocarbons formed during pyrolysis(S_(2)),and mineral contents,the enrichment characteristics and controlling factors of shale oil in an overmature area were investigated.The results indicate that:(1)TOC,S_(1),and S_(2)associated with shale oil enrichment exhibit a significant 173×10^(3)a obliquity amplitude modulation cycle;(2)Quartz and illite/smectite mixed-layer contents related to lithological composition show a significant 405×10^(3)a long eccentricity cycle;(3)Comparative studies with the high-maturity GY3HC well and moderate-maturity ZY1 well reveal distinct in-situ enrichment characteristics of shale oil in the overmature Qingshankou Formation,with a significant positive correlation to TOC,indicating that high TOC is a key factor for shale oil enrichment in overmature areas;(4)The sedimentary thickness of 12-13 m corresponding to the 173×10^(3)a cycle can serve as the sweet spot interval height for shale oil development in the study area,falling within the optimal fracture height range(10-15 m)generated during hydraulic fracturing of the Qingshankou shale.Orbitally forced climate changes not only controlled the sedimentary rhythms of organic carbon burial and lithological composition in the Songliao Basin but also influenced the enrichment characteristics and sweet spot distribution of Gulong shale oil.展开更多
Objective:Investigating the effects and molecular mechanisms of nursing interventions based on environmental enrichment on cognitive function in ischemic stroke rats.Methods:A total of 30 Sprague-Dawley(SD)rats belong...Objective:Investigating the effects and molecular mechanisms of nursing interventions based on environmental enrichment on cognitive function in ischemic stroke rats.Methods:A total of 30 Sprague-Dawley(SD)rats belonging to the same batch were randomly divided into 3 groups(n=10)using a random number table:Sham Surgery Control Group(Sham),Ischemia-Reperfusion(I/R)Group,and Ischemia-Reperfusion Group with Environmental Enrichment Intervention(I/R+EEI).The expression levels of brain-derived neurotrophic factor(BDNF)protein in the hippocampus region were measured and compared among different groups.Results:Compared with the Sham group,the I/R group showed significantly reduced learning and memory abilities,with notably lower BDNF levels(P<0.05).Compared to the I/R group,the I/R+EEI group exhibited significantly improved learning and memory abilities as well as increased BDNF protein levels(P<0.05).Conclusions:Abnormal BDNF protein secretion may be the molecular mechanism of cognitive dysfunction due to hippocampal neuronal damage in ischemia-reperfusion,and modifying this neurotransmitter’s secretion can effectively improve cognitive function in ischemia-reperfusion rats.展开更多
Based on the petroleum exploration in the Cretaceous Qingshankou Formation,northern Songliao Basin,NE China,integrated with seismic,drilling and logging data,this study investigates the characteristics and genetic mec...Based on the petroleum exploration in the Cretaceous Qingshankou Formation,northern Songliao Basin,NE China,integrated with seismic,drilling and logging data,this study investigates the characteristics and genetic mechanisms of orderly distribution and the differential enrichment patterns of conventional and unconventional hydrocarbons in the formation.Key findings involve five aspects.First,the conventional and unconventional hydrocarbons coexist orderly.Laterally,conventional oil,tight oil,and shale oil form a pattern of orderly accumulation from basin margins to the center.Vertically,shale oil,tight oil,and conventional oil develop progressively upward.Second,the coupled tectonic-sedimentary processes govern sedimentary facies differentiation and diagenesis,influencing reservoir physical properties and lithology,thereby controlling the orderly distribution of conventional and unconventional hydrocarbons in space.Third,the coupling of source rock hydrocarbon generation evolution,fault sealing capacity,and reservoir densification determines the orderly coexistence pattern of conventional and unconventional hydrocarbons.Fourth,sequential variations in reservoir physical properties generate distinct dynamic fields that regulate hydrocarbon orderly accumulation.Fifth,enrichment controls are different depending on hydrocarbon types:buoyancy-driven,fault-transport,sandbody-connected,and trap-concentrated,for above-source conventional oil;overpressure-driven,fault-transport,multi-stacked sandbodies,and quasi-continuous distribution for near-source tight oil and gas;self-sourced reservoirs,retention through self-sealing,in-situ accumulation or micro-migration driven by hydrocarbon-generation overpressure for inner-source shale oil.From exploration practices,these findings will effectively guide the integrated deployment and three-dimensional exploration of conventional and unconventional hydrocarbon resources in the Qingshankou Formation,northern Songliao Basin.展开更多
The marine-continental transitional shale of the Upper Permian Longtan Formation is widely distributed in Hunan and shows significant exploration potential.Frequent changes in lithofacies have however notably influenc...The marine-continental transitional shale of the Upper Permian Longtan Formation is widely distributed in Hunan and shows significant exploration potential.Frequent changes in lithofacies have however notably influenced the shale gas enrichment.The strata of the Longtan Formation in the Shaoyang Depression,central Hunan,were taken as the study object for this project.Three lithofacies assemblages were identified:shale interbedded with sandstone layer(SAL),sandstone interbedded with shale layer(ASL)and laminated shale layer(LSL).The SAL shale shows significant variability in hydrocarbon generation potential,which leads to shale gas characterized by'hydrocarbon generation in high total organic carbon(TOC)shale,retention in low TOC shale and accumulation in sandstone'.The ASL shale,influenced by the redox conditions of the depositional environment,shows a lower concentration of organic matter.This results in an enrichment model of'hydrocarbon generation and accumulation in shale,with sealing by sandstone'.The laminar structure of LSL shale causes both quartz and clay minerals to control the reservoir.Shale gas is characterized by'hydrocarbon generation in mud laminae,retention and accumulation in silty laminae,with multiple intra-source migration paths'.In the marine-continental transitional shale gas system,the enrichment intervals of different types of shale gas reservoirs exhibit significant variability.展开更多
The lamina(combination)types,reservoir characteristics and shale oil occurrence states of organic-rich shale in the Triassic Yanchang Formation Chang 73 sub-member in the Ordos Basin were systematically investigated t...The lamina(combination)types,reservoir characteristics and shale oil occurrence states of organic-rich shale in the Triassic Yanchang Formation Chang 73 sub-member in the Ordos Basin were systematically investigated to reveal the main controlling factors of shale oil occurrence under different lamina combinations.The differential enrichment mechanisms and patterns of shale oil were discussed using the shale oil micro-migration characterization and evaluation methods from the perspectives of relay hydrocarbon supply,stepwise migration,and multi-stage differentiation.The results are obtained in five aspects.First,Chang 73 shale mainly develops five types of lamina combination,i.e.non-laminated shale,sandy laminated shale,tuffaceous laminated shale,mixed laminated shale,and organic-rich laminated shale.Second,shales with different lamina combinations are obviously different in the reservoir space.Specifically,shales with sandy laminae and tuffaceous laminae have a large number of intergranular pores,dissolution pores and hydrocarbon generation-induced fractures.The multi-scale pore and fracture system constitutes the main place for liquid hydrocarbon occurrence.Third,the occurrence and distribution of shale oil in shale with different lamina combinations are jointly controlled by organic matter abundance,reservoir property,thermal evolution degree,mineral composition and laminae scale.The micro-nano-scale pore-fracture networks within shales containing rigid laminae,particularly sandy and tuffaceous laminations,primarily contain free-state light hydrocarbon components.In contrast,adsorption-phase heavy hydrocarbon components predominantly occupy surfaces of organic matter assemblages,clay mineral matrices,and framework mineral particulates.Fourth,there is obvious shale oil micro-migration between shales with different lamina combinations in Chang 73.Generally,such micro-migration is stepwise in a sequence of organic-rich laminated shale→tuffaceous laminated shale→mixed laminated shale→sandy lamiated shale→non-laminated shale.Fifth,the relay hydrocarbon supply of organic matter under the control of the spatial superposition of shales with various laminae,the stepwise migration via multi-scale pore and fracture network,and the multi-differentiation in shales with different lamina combinations under the control of organic-inorganic interactions fundamentally decide the differences of shale oil components between shales with different lamina combinations.展开更多
基金Supported by the National Natural Science Foundation of China for Young Scientists Fund(No.42102158)the Jilin Province Science and Technology Development Plan Project(No.20240101065JC)+2 种基金the project of Education Department of Jilin Provincial(No.JJKH20241292KJ)the Natural Science Foundation of Sichuan Province(No.2024NSFSC0087)Supported by Graduate Innovation Fund of Jilin University(No.2024CX238).
文摘The quest for enrichment model of continental shale oil in the Zhanhua Sag of the JiyangDepression in the Bohai Bay Basin to provide reference for exploration and development requires acomprehensive approach. Therefore, this study employs rock pyrolysis, Scanning Electron Microscopy(SEM), X-Ray diffraction analysis (XRD), Nuclear Magnetic Resonance (NMR), and other experiments toanalyze the conditions for shale oil enrichment and establish its patterns. The results show that favorablehydrocarbon generation potential and appropriate thermal maturation degree control “in situ enrichment”;while the storage capacity and the mobility of shale oil determine “migration enrichment.” In the process,the TOC governs the oil-generating capacity of shale with medium to large pores and microfractures servingas the main enrichment spaces and migration pathways for shale oil. Based on the deposition model, thestudy area can be divided into five lithofacies stages (I-algal limestone, II-laminated marl, III-laminatedrecrystallized limestone, IV-laminated mudstones, and IV-blocky calcareous mudstones). Integrating thegeochemical parameters into the sedimentary patterns makes it clear that the study area underwent two phasesof hydrocarbon expulsion during the thermal evolution of source rocks (Stage II: 3 060–3 120 m and StageIV: 3 020–3 040 m). However, judging by the observed TOC (2% to 5.6%), thermal maturity (Ro>0.8%),S1 (>2 mg/g) and OSI (>100 mg/g) as well as moderate basin size, climate, and quantity of terrestrial input,the blocky calcareous mudstones (Stage IV) have better oil-prone characteristics and potential to generate asubstantial quantity of hydrocarbons at this stage. More so, with a brittleness index exceeding 60%, it exhibitsfavorable fracturability accounting for the main controlling factors and enrichment patterns of shale oil in thearea. Hence, this study further enriches and develops the theoretical understanding of shale oil enrichment inthe area, provides valuable insights for future exploration of continental shale oil in eastern China and othersimilar basin around the world.
基金supported by the National Natural Science Foundation of China(61902095).
文摘Biomedical big data,characterized by its massive scale,multi-dimensionality,and heterogeneity,offers novel perspectives for disease research,elucidates biological principles,and simultaneously prompts changes in related research methodologies.Biomedical ontology,as a shared formal conceptual system,not only offers standardized terms for multi-source biomedical data but also provides a solid data foundation and framework for biomedical research.In this review,we summarize enrichment analysis and deep learning for biomedical ontology based on its structure and semantic annotation properties,highlighting how technological advancements are enabling the more comprehensive use of ontology information.Enrichment analysis represents an important application of ontology to elucidate the potential biological significance for a particular molecular list.Deep learning,on the other hand,represents an increasingly powerful analytical tool that can be more widely combined with ontology for analysis and prediction.With the continuous evolution of big data technologies,the integration of these technologies with biomedical ontologies is opening up exciting new possibilities for advancing biomedical research.
基金Supported by the National Natural Science Foundation of China(U22B6004)Project of PetroChina Research Institute of Petroleum Exploration and Development(2022yjcq03)Core Technology Key Project of China Petroleum Changqing Oilfield Company(KJZX2023-01).
文摘In addition to the organic matter type,abundance,thermal maturity,and shale reservoir space,the preservation conditions of source rocks play a key factor in affecting the quantity and quality of retained hydrocarbons in source rocks of lacustrine shale,yet this aspect has received little attention.This paper,based on the case analysis,explores how preservation conditions influence the enrichment of mobile hydrocarbons in shale oil.Research showns that good preservation conditions play three key roles.(1)Ensure the retention of sufficient light hydrocarbons(C_(1)–C_(13)),medium hydrocarbons(C_(14)–C_(25))and small molecular aromatics(including 1–2 benzene rings)in the formation,which enhances the fluidity and flow of shale oil;(2)Maintain a high energy field(abnormally high pressure),thus facilitating the maximum outflow of shale oil;(3)Ensure that the retained hydrocarbons have the miscible flow condition of multi-component hydrocarbons(light hydrocarbons,medium hydrocarbons,heavy hydrocarbons,and heteroatomic compounds),so that the heavy hydrocarbons(C_(25+))and heavy components(non-hydrocarbons and asphaltenes)have improved fluidity and maximum flow capacity.In conclusion,in addition to the advantages of organic matter type,abundance,thermal maturity,and reservoir space,good preservation conditions of shale layers are essential for the formation of economically viable shale oil reservoirs,which should be incorporated into the evaluation criteria of shale oil-rich areas/segments and considered a necessary factor when selecting favorable exploration targets.
基金supported by the National Natural Science Foundation of China(No.52070149)Shaanxi Innovative Research Team for Key Science and Technology(No.2023-CXTD-36)+1 种基金Shaanxi Province Key Program for International S&T Cooperation Projects(No.2024GH-ZDXM-04)the Bureau of Science and Technology of Xi’an City of China(No.23SFSF0011).
文摘Anaerobic ammonia oxidation(Anammox)is an economical and sustainablewastewater nitrogen removal technology,and its application in the mainstream process is the inevitable trend of the development of Anammox.However,how to effectively enriching Anammox bacteria from the activated sludge remains challenging and restricts its extensive applications.In this study,the rapid and efficient enrichment of Anammox bacteriawas achieved by raising the reflux ratio and nitrogen loading rate(NLR)using conventional activated sludge as the inoculant.In the screening phase(days 1–90),the reflux ratio was increased to discharge partial floc sludge,resulting in the relative abundance of Candidatus Brocadiaceae increased from0.04%to 22.54%,which effectively reduced thematrix and spatial competition between other microorganisms and Anammox bacteria.On day 90,the stoichiometric ratio of the Anammox process closely approached the theoretical value of 1:1.32:0.26,indicating that the Anammox reaction was the primary nitrogen removal process in the system.In the enrichment phase(days 91–238),the NLR increased from 0.43 to 1.20 kgN/(m^(3)·d)and removal efficiency was 71.89%,resulting in the relative abundance of Candidatus Brocadiaceae increased to 61.27%on day 180.The reactor operated steadily from days 444 to 498,maintaining the nitrogen removal rate(NRR)of 3.00 kgN/(m^(3)·d)and achieving successful sludge granulation with the particle size of 392.4μm.In short,this study provided a simple and efficient approach for enriching Anammox bacteria from the activated sludge,supporting to start an Anammox process efficiently.
基金Supported by the PetroChina Science&Technology Special Project(2023ZZ21YJ04)PetroChina Gas Reservoir Evaluation Project(20230304-08)。
文摘Based on the basic data of drilling,logging,testing and geological experiments,the geological characteristics of the Permian Dalong Formation marine shales in the northern Sichuan Basin and the factors controlling shale gas enrichment and high yield are studied.The results are obtained in four aspects.First,the high-quality shale of the Dalong Formation was formed after the deposition of the Permian Wujiaping Formation,and it is developed in the Kaijiang-Liangping trough in the northern part of Sichuan Basin,where deep-water continental shelf facies and deep-water reduction environment with thriving siliceous organisms have formed the black siliceous shale rich in organic matter.Second,the Dalong Formation shale contains both organic and inorganic pores,with stratification of alternated brittle and plastic minerals.In addition to organic pores,a large number of inorganic pores are developed even in ultra-deep(deeper than 4500 m)layers,contributing a total porosity of more than 5%,which significantly expands the storage space for shale gas.Third,the limestone at the roof and floor of the Dalong Formation acted as seal rock in the early burial and hydrocarbon generation stage,providing favorable conditions for the continuous hydrocarbon generation and rich gas preservation in shale interval.In the later reservoir stimulation process,it was beneficial to the lateral extension of the fractures,so as to achieve the optimal stimulation performance and increase the well-controlled resources.Combining the geological,engineering and economic conditions,the favorable area with depth less than 5500 m is determined to be 1800 km2,with resources of 5400×10^(8) m^(3).Fourth,the shale reservoirs of the Dalong Formation are thin but rich in shale gas.The syncline zone far away from the main faults in the high and steep tectonic zone,eastern Sichuan Basin,with depth less than 5500 m,is the most favorable target for producing the Permian shale gas under the current engineering and technical conditions.It mainly includes the Nanya syncline,Tanmuchang syncline and Liangping syncline.
文摘Tropical coastal ecosystems,vital habitats for economically significant marine species,face increasing threats from eutrophication driven by human-induced nutrient pollution.This study investigated the metabolomic profile of Anadara granosa,a marine bivalve,to identify metabolites indicative of eutrophic conditions.Conducted in Cirebon Bay,Indonesia,the research spanned five locations reflecting varying eutrophication levels.Bottom-layer water samples were analyzed for pH,dissolved oxygen,salinity,water transparency,and nutrient concentrations(phosphate,nitrate,ammonia).Proton-Nuclear Magnetic Resonance(Proton-NMR)metabolomics of A.granosa revealed metabolite variations linked to water quality.Eutrophic conditions,prevalent in the bay’s northern side,were characterized by inorganic nitrogen levels above 0.2 mg L^(-1) and phosphate exceeding 0.024 mg L^(-1).These were linked to urban,aquaculture,and agricultural runoff,driving increased phytoplankton biomass and reduced salinity variability.Metabolomic analysis identified inosine and dimethylglycine as potential biomarkers.Elevated inosine levels in eutrophic environments suggest increased cellular activity due to greater food availability,while higher dimethylglycine concentrations detected in low-nutrient as its role in osmotic regulation.Both metabolites correlated with ammonia concentrations(P<0.05,R^(2)=0.631),a key contributor to eutrophication.These findings highlight inosine and dimethylglycine as effective indicators of nutrient enrichment,providing insights into eutrophication’s impact on coastal ecosystems and A.granosa’s physiological responses.
基金funded by the National Key Research and Development Program of China(No.20022YFC3102405)the National Natural Science Foundation of China(Nos.42425004,32371665)the Natural Science Foundation of Guangdong Province(Nos.2022A1515011461,2022A1515011831)。
文摘Macroalgae dominate nutrient dynamics and function as high-value foods for microbial,meio-and macrofaunal communities in coastal ecosystems.Because of this vital role,it is important to clarify the physiological information associated with environmental changes as it reflects their growth potential.To evaluate the effects of the changes in salinity and nutrients,the photosynthetic efficiency of a green macroalga Ulva fasciata from the Daya Bay was tested at a range of salinity(i.e.,31 to 10 psu)and nitrogen content(i.e.,5 to 60μmol L^(-1)).The results showed that cellular chlorophyll a(Chl a),carbohydrate and protein contents of U.fasciata were increased due to reduced salinity,and were decreased by interactive nitrogen enrichment.Within a short culture period(i.e.,18 h),the reduced salinity decreased the maximum photosynthetic efficiency(rETRmax and Pmax)derived from the rapid light response curve and photosynthetic oxygen evolution rate versus irradiance curve,respectively,as well as the saturation irradiance(E_(K)).This reducing effect diminished with enlonged cultivation time and reversed to a stimulating effect after 24 h of cultivation.The nitrogen enrichment stimulated the rETRmax and Pmax,as well as the E_(K),regardless of salinity,especially within short-term cultivation period(i.e.,<24 h).In addition,our results indicate that seawater freshening lowers the photosynthetic efficiency of U.fasciata in the short term,which is mitigated by nitrogen enrichment,but stimulates it in the long term,providing insight into how macroalgae thrive in coastal or estuarine waters where salinity and nutrients normally covary strongly.
基金funded by a grant from the National Natural Science Foundation of China(Grant No.41772130)the Natural Science Foundation of Jiangsu Province(Grant No.BK20210521)+4 种基金the Fundamental Research Funds for the Central Universities(Grant No.2021QN1061)‘Energy and Environment Youth Talent Training Program’by China’s Energy Society,China’s Environmental Protection Foundation and the Beijing Energy Society(Grant No.RCJH2022081)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.KYCX25_2782)the Graduate Innovation Program of China University of Mining and Technology(No.2025WLKXJ003)the Fundamental Research Funds for the Central Universities(No.202500044)。
文摘Recent studies have highlighted the presence of lithium enrichment in coals within the Ordos Basin,which has garnered significant attention due to the potential economic value.However,most research has concentrated primarily on the coal seams of the Taiyuan and Shanxi formations,with limited reports on the Benxi Formation.To address this gap,our study focused on elucidating the geochemical characteristics and origins of the lithium enrichment in the No.8 coal of the Benxi Formation through the use of optical microscopy and inductively coupled plasma mass spectrometry(ICP-MS).The results showed that the No.8 coal was a bituminous coal,characterized by medium ash yield,low volatile matter,high total sulfur content and medium vitrinite proportion.The No.8 coal was enriched in Li(average 91.7 ppm,CC=6.55)and Zr(average 191 ppm,CC=5.30),the unusual enrichment of Li being primarily located in the middle of the coal.The minerals in the coal were predominantly clay minerals,along with minor amounts of pyrite,quartz and calcite.The occurrence mode of Li in the No.8 coal was associated with aluminosilicate minerals,presumably kaolinite.Based on geochemical characteristics,the sedimentary source of the No.8 coal was intermediate-felsic volcanic rock from the Yinshan oldland.The enrichment of Li can be attributed to the supply of terrestrial debris from the Yinshan oldland and the depositional environment.Our analysis identifies three distinct stages of lithium enrichment,emphasizing the critical role played by the terrestrial debris,as well as the acidic to partially reducing conditions,in facilitating this process.In conclusion,our study sheds light on the mechanisms underlying lithium enrichment in the No.8 coal of the Benxi Formation,highlighting the significance of geological factors in shaping the distribution and concentration of critical metals in coal.
基金supported by the National Natural Science Foundation of China (Nos.42077193 and 21976034)the National Key R&D Program (Nos.2022YFC3702600 and 2022YFC3702601)the Shanghai Natural Science Foundation (23ZR1479700).
文摘Ultrasonic humidifiers are commonly used in households to maintain indoor humidity and generate a large number of droplets or spray aerosols.However,there have been various health concerns associated with humidifier use,largely due to aerosols generated during operation.Here,we investigated the size distribution,chemical composition,and charged fraction of aerosol particles emitted from commercial ultrasonic humidifiers.Heavy metals in water used for humidifiers were found to be highly enriched in the ultrasonic humidifier aerosols(UHA),with the enrichment factors ranging from 102 to 107.This enrichment may pose health concerns for the building occupants,as UHA concentrations of up to 106 particles/cm^(3) or 3 mg/m^(3) were observed.Furthermore,approximately 90%of UHA were observed to be electrically charged,for the first time according to our knowledge.Based on this discovery,we proposed and tested a newmethod to remove UHA by using a simple electrical field.The designed electrical field in this work can efficiently remove 81.4%of UHA.Therefore,applying this electrical field could be an effective method to significantly reduce the health risks by UHA.
基金supported by the National Natural Science Foundation of China (No. 42277103)the Shenzhen Science and Technology Innovation Committee, China (Nos. JCYJ20240813095659001 and KCXFZ20240903094205008)+1 种基金the Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, China (No. 2023B1212060002)the Guangdong Provincial Key Laboratory of Environmental Health and Land Resource, China (No. 2020B121201014) and the high level special funds, China (Nos. G03050K001 and G030290001)。
文摘The efficacy of DNA sequencing,particularly long reads nanopore sequencing,is critically dependent on the amount and quality of the input DNA.However,extracting high concentrations of DNA from low biomass samples,especially from solid matrices,presents significant challenges,this limitation not only substantially hampers the scope of environmental microbiology studies but also makes enhancing DNA yield indispensable in many instances.Therefore,in this study,we systematically evaluated the impact of four different DNA enrichment methods on both amplicon and metagenomic community analyses of solid-phase,low-biomass samples:permafrost soil and biofilm of sand filter.These methods include multiple displacement amplification(MDA),centrifugal filtration(CF),freeze vacuum drying at(FVD)as well as vacuum centrifugal at 35,45,and 60°C(namely VC35,VC45,VC60).Our results indicate that FVD was the most effective for increasing DNA concentration,while VC methods best preserved DNA fragment length.In contrast,the widely used MDA and CF methods exhibited biases,preferentially enriching low-GC content sequences,which affected both assembly and annotation outcomes.Metagenomic assembly from MDA and CF samples was suboptimal,with fewer contigs and no middle quality MAGs recovered compared to other methods.Community composition analysis revealed significant shifts across all enrichment methods,with Sphingomonas and Sphingorhabdus genera could be obviously enriched.These findings highlight the necessity and importance of carefully selecting DNA enrichment methods to ensure reliable metagenomic investigation of low-biomass environmental samples.
基金supported by the National Natural Science Foundation of China(No.42107094)Sichuan Science and Technology Program(No.2023NSFSC0806)the Geology Bureau project of Xinjiang Uygur Autonomous Region(Nos.XGMB202356 and XGMB202358).
文摘The geogenic enrichment of arsenic(As)extensively occurred in the riverine systems from the Qinghai-Tibetan Plateau under active geothermal discharge and chemical weathering conditions,while little is known about how dissolved organic matter(DOM)transformation regulates the aquatic As concentrations.The present study revealed that the DOM components from the Singe Tsangpo River(STR)basin primarily consisted of protein-like components(81.30%±6.48%),with the microbially-endogenous production being a predominant source under the control of temperature and glacier-runoff recharge along the river flow path.Notably,the chemical weathering processes have significantly facilitated the enhancement of humic-like components in the river water.Besides,the groundwater DOM characteristics were predominantly influenced by the mobilization of sedimentary organic matter and the introduction of allochthonous DOM resulting from surface-water recharge.Interestingly,humic-like components facilitated As enrichment through complexation and competitive adsorption effects in both surface water and groundwater under oxidizing conditions,whichwas supported by the significant positive correlations between As and humiclike component(R^(2)=0.31/0.65,P<0.05/0.01)and the concurrent mobilization of As and humic-like components from sediment incubation experiments.Moreover,the Structural Equation Modeling analysis revealed a stronger contribution of humic-like components to the As enrichment in the groundwater compared with surface water,possibly due to the relatively strongermicrobial activity and enhanced mobilization of humic-like components in alluvial aquifers.The present study thus provided new insights into the transformation of DOM and its important role in facilitating As enrichment in the aquatic environment from alpine river basins.
基金financially supported by the National Natural Science Foundation of China(Grant No.42002150)the Open Foundation of Cooperative Innovation Center of Unconventional Oil and Gas,Yangtze University(Ministry of Education&Hubei Province,Grant No.UOG2024-12)+1 种基金the Open Foundation Project of the Key Laboratory of Polar Geology and Marine Mineral Resources(China University of Geosciences,Beijing,China),Ministry of Education(Grant No.PGMR-2023-201)the National Key Research Program of China(Grant No.2017ZX05032-002)。
文摘Compared to discrete continental marginal basins,the mechanisms of hydrocarbon migration and enrichment in transform continental marginal basins are poorly understood.In this study,we conducted a comprehensive analysis of the main source rocks,reservoirs,and vertical migration pathways within the Rovuma(RB)and Tanzania(TB)basins in East Africa utilizing drilling,logging,seismic,and geochemical data.The results indicate that the enhanced preservation conditions of the Lower Jurassic source rocks in the southwest could lead to the discovery of large natural gas fields in the southern TB and RB.The primary reservoir is a deep-water turbidite sandstone.Due to topographic differences,the expanse of the turbidite sandstones in the RB is significantly larger than those in Tanzania.The main vertical migration pathways are the western boundary fault zone of the Kerimbas Graben(WBFZ)and the Seagap fault zone(SFZ).In the RB,natural gas migrates vertically along the WBFZ and preferentially accumulates in the deep-water turbidite sandstones of the footwall under the control of the fluid potential.Conversely,in the southern TB,the deep natural gas first migrates upward along the SFZ,then moves along the shallow branch faults in the sandstones on both sides of the SFZ.
基金financially supported by NKPRP of China(2022YFC2903504)the National Natural Science Foundation of China(Grant Nos.42272069,42303023,91962212 and 41872075)+2 种基金Postdoctoral Fellowship Program of CPSF(GZC20231005)Qinghai Jinchuan Mining Co.,Ltd.R&D Project(0511-HHKY-FY-[2023])Key Laboratory of Strategic Mineral Resources of the Upper Yellow River,Ministry of Natural Resources(YSMRKF202202).
文摘The Jinchuan magmatic Ni-Cu-PGE deposit is the largest single Ni sulfide deposit in the world.It consists primarily of orebody-24 in segment I,and orebody-1 and orebody-2 in segment II.The contents of platinum-group elements(PGE)in these orebodies decrease significantly from west to east across the deposit.However,the PGE characteristics of platinum-group minerals(PGM)and alloys,as well as their roles during mineralization in different orebodies,remain unclear.In this study,PGM and alloy occurrences in orebody-24 and orebody-2 had been observed by scanning electron microscopy(SEM)and spherical-aberration corrected scanning transmission electron microscope(Cs-STEM).The PGE contents were analyzed by SEM‒EDS for submicron-scale PGM and alloys.The results show that olivine in lherzolite mainly hosts PGM of PtTe and PdBi2,whereas pyroxene contains small amounts of PdBiTe.Pyrrhotite predominantly hosts(Ir-Rh-Pt)AsS,Pd(BiTe),and other PGM,as well as PtSn and PtOs alloys.Pentlandite mainly encloses Pd(BiTe),PdBi/PdBi2 and other PGM,as well as PtSn and PtFe alloys.Chalcopyrite primarily encloses PdBi/PdBi2,and other PGM,along with PtSn and IrOs.Distinct distribution patterns of PGE in PGM and alloys had been observed between different orebodies.Orebody-24 contains more(Ir-Rh-Pt)AsS minerals and PtFe/PtSn alloy grains,whereas orebody-2 has a higher proportion of Pt-and Pd-bearing PGM.The presence of euhedral alloys in silicate minerals from orebody-24 suggests that its parent magma had a higher PGE content before sulfide saturation than that of orebody-2.More than 90%of PGM and alloys in both orebody-24 and orebody-2 contain Pt and Pd,emphasizing their contributions to the elevated Pt and Pd concentrations.The different PGE distributions of PGM and alloys in the two orebodies suggest that thermodynamic conditions(fO2 and fS2)and semimetals,especially As,play critical roles in controlling PGE behavior and occurrence.
基金financially supported by the National Natural Science Foundation of China(Grant No.42072150)and we thank the sponsors of these projects.
文摘The relationship between marine transgression and the distribution of lacustrine organic matter has restricted shale oil and gas exploration for decades.In this study,the research objective is to analyze the sedimentary environment and evaluate its influence on organic matter in transgressive lacustrine shale.The study uses various analyses including total organic carbon(TOC),Rock-Eval pyrolysis,gas chromatography-mass spectrometry(GC-MS),trace element and isotope analysis.Finally,the study proposes an enrichment model for organic matter.The lacustrine shale of the second member of the Funing Formation(E_(1)f^(2))is divided into three sequences.The results indicate that the depositional environment of the organic matter during this period was an arid and humid,reduced,closed,rift lake basin.In the first sequence,high salinity resulted from increased evaporation,leading to low primary biological productivity.At this time,the lake basin belonged to a salinized closed lake basin.Intermittent transgressions began in the second sequence,with the deep lake area still being dominated by a reducing environment.The third sequence saw the environment evolve into a closed lake basin characterized by a warm and humid freshwater environment with high primary productivity.Marine transgressions introduce a substantial amount of marine plankton,nutrient elements,as well as more CO_(2) and CO_(3)^(2−)into the lake,leading to increased primary productivity.The sedimentary model for transgressive lacustrine source rocks proposed here serves as an example for similar transgressive lake basins.
基金supported by the National Natural Science Foundation of China(Nos.22338005,21977015)。
文摘Receptor tyrosine kinases(RTKs)are biological enzymes expressed on cell membranes that can influence cellular signaling,and their overexpression in tumor cells makes them a key route to assess relevant tumor processes.The development of a delivery system that targets and accumulates in RTKs overexpressing-cells at the on-target site is significant for the monitoring of tumor progression and clinical applications through longer tumor site signaling response under low injection frequency.Here,a host-vip nanoscale fluorescent probe SNI@ZIF-8 based on zeolitic imidazolate framework-8(ZIF-8)and a fluorescent probe SNI constructed from receptor tyrosine kinase inhibitor was proposed and prepared for targeting RTKs and enabling prolonged fluorescence imaging in vivo.The folded conformation of the probe SNI resulted in low background fluorescence,and the unfolding of the SNI conformation upon insertion of the RTKs active pocket showed significant fluorescence enhancement thus enabling real-time detection of RTKs.The host-vip system SNI@ZIF-8 could release vip molecules due to the presence of the enzyme,emphasizing the reporting of stable fluorescent signals over time under low injection frequency.SNI@ZIF-8 could provide a signal response on the cell membrane of RTKs overexpressing cells without interference from other substances,and provided a longer fluorescent signal than SNI at equivalent number of injections in tumor-bearing mice.The host-vip system SNI@ZIF-8,with its obvious tumor site enrichment ability and clear fluorescence imaging ability,could be successfully applied to the detection of RTKs on cell membranes in biological systems,providing a new strategy for determining the process of tumor development in clinical applications.
基金Supported by the National Natural Science Foundation of China(42372162,4244205C)Project of"Solving Problems by Listing Talents"in Heilongjiang Province(2022-JS-1740,2022-JS-1853)Project on the Theory of Oil and Gas Enrichment from the Interaction of Earth's Multiple Spheres(THEMSIE04010103).
文摘Taking the GY8HC well in the Gulong Sag of the Songliao Basin,NE China,as an example,this study utilized high-precision zircon U-Pb ages from volcanic ashes and AstroBayes method to estimate sedimentation rates.Through spectral analysis of high-resolution total organic carbon content(TOC),laboratory-measured free hydrocarbons(S_(1)),hydrocarbons formed during pyrolysis(S_(2)),and mineral contents,the enrichment characteristics and controlling factors of shale oil in an overmature area were investigated.The results indicate that:(1)TOC,S_(1),and S_(2)associated with shale oil enrichment exhibit a significant 173×10^(3)a obliquity amplitude modulation cycle;(2)Quartz and illite/smectite mixed-layer contents related to lithological composition show a significant 405×10^(3)a long eccentricity cycle;(3)Comparative studies with the high-maturity GY3HC well and moderate-maturity ZY1 well reveal distinct in-situ enrichment characteristics of shale oil in the overmature Qingshankou Formation,with a significant positive correlation to TOC,indicating that high TOC is a key factor for shale oil enrichment in overmature areas;(4)The sedimentary thickness of 12-13 m corresponding to the 173×10^(3)a cycle can serve as the sweet spot interval height for shale oil development in the study area,falling within the optimal fracture height range(10-15 m)generated during hydraulic fracturing of the Qingshankou shale.Orbitally forced climate changes not only controlled the sedimentary rhythms of organic carbon burial and lithological composition in the Songliao Basin but also influenced the enrichment characteristics and sweet spot distribution of Gulong shale oil.
基金supported by 2023 Jiangsu Xuzhou Medical Higher Vocational School Natural Science Project(No.2023ZZX09)。
文摘Objective:Investigating the effects and molecular mechanisms of nursing interventions based on environmental enrichment on cognitive function in ischemic stroke rats.Methods:A total of 30 Sprague-Dawley(SD)rats belonging to the same batch were randomly divided into 3 groups(n=10)using a random number table:Sham Surgery Control Group(Sham),Ischemia-Reperfusion(I/R)Group,and Ischemia-Reperfusion Group with Environmental Enrichment Intervention(I/R+EEI).The expression levels of brain-derived neurotrophic factor(BDNF)protein in the hippocampus region were measured and compared among different groups.Results:Compared with the Sham group,the I/R group showed significantly reduced learning and memory abilities,with notably lower BDNF levels(P<0.05).Compared to the I/R group,the I/R+EEI group exhibited significantly improved learning and memory abilities as well as increased BDNF protein levels(P<0.05).Conclusions:Abnormal BDNF protein secretion may be the molecular mechanism of cognitive dysfunction due to hippocampal neuronal damage in ischemia-reperfusion,and modifying this neurotransmitter’s secretion can effectively improve cognitive function in ischemia-reperfusion rats.
基金Supported by the PetroChina Science and Technology Major Project(2023ZZ15).
文摘Based on the petroleum exploration in the Cretaceous Qingshankou Formation,northern Songliao Basin,NE China,integrated with seismic,drilling and logging data,this study investigates the characteristics and genetic mechanisms of orderly distribution and the differential enrichment patterns of conventional and unconventional hydrocarbons in the formation.Key findings involve five aspects.First,the conventional and unconventional hydrocarbons coexist orderly.Laterally,conventional oil,tight oil,and shale oil form a pattern of orderly accumulation from basin margins to the center.Vertically,shale oil,tight oil,and conventional oil develop progressively upward.Second,the coupled tectonic-sedimentary processes govern sedimentary facies differentiation and diagenesis,influencing reservoir physical properties and lithology,thereby controlling the orderly distribution of conventional and unconventional hydrocarbons in space.Third,the coupling of source rock hydrocarbon generation evolution,fault sealing capacity,and reservoir densification determines the orderly coexistence pattern of conventional and unconventional hydrocarbons.Fourth,sequential variations in reservoir physical properties generate distinct dynamic fields that regulate hydrocarbon orderly accumulation.Fifth,enrichment controls are different depending on hydrocarbon types:buoyancy-driven,fault-transport,sandbody-connected,and trap-concentrated,for above-source conventional oil;overpressure-driven,fault-transport,multi-stacked sandbodies,and quasi-continuous distribution for near-source tight oil and gas;self-sourced reservoirs,retention through self-sealing,in-situ accumulation or micro-migration driven by hydrocarbon-generation overpressure for inner-source shale oil.From exploration practices,these findings will effectively guide the integrated deployment and three-dimensional exploration of conventional and unconventional hydrocarbon resources in the Qingshankou Formation,northern Songliao Basin.
基金financial support from the National Natural Science Foundation of China(Grant Nos.U23B20155 and 42202140)the Science and Technology Innovation Program of Hunan Province(2023RC1021)+1 种基金the China Geological Survey(DD20221659)the Science and Technology Bureau,Changsha,China(kq2208261)。
文摘The marine-continental transitional shale of the Upper Permian Longtan Formation is widely distributed in Hunan and shows significant exploration potential.Frequent changes in lithofacies have however notably influenced the shale gas enrichment.The strata of the Longtan Formation in the Shaoyang Depression,central Hunan,were taken as the study object for this project.Three lithofacies assemblages were identified:shale interbedded with sandstone layer(SAL),sandstone interbedded with shale layer(ASL)and laminated shale layer(LSL).The SAL shale shows significant variability in hydrocarbon generation potential,which leads to shale gas characterized by'hydrocarbon generation in high total organic carbon(TOC)shale,retention in low TOC shale and accumulation in sandstone'.The ASL shale,influenced by the redox conditions of the depositional environment,shows a lower concentration of organic matter.This results in an enrichment model of'hydrocarbon generation and accumulation in shale,with sealing by sandstone'.The laminar structure of LSL shale causes both quartz and clay minerals to control the reservoir.Shale gas is characterized by'hydrocarbon generation in mud laminae,retention and accumulation in silty laminae,with multiple intra-source migration paths'.In the marine-continental transitional shale gas system,the enrichment intervals of different types of shale gas reservoirs exhibit significant variability.
基金Supported by the National Natural Science Foundation of China(42302184)Innovation Group Project of Basic Research in Gansu Province,China(22JR5RA045)。
文摘The lamina(combination)types,reservoir characteristics and shale oil occurrence states of organic-rich shale in the Triassic Yanchang Formation Chang 73 sub-member in the Ordos Basin were systematically investigated to reveal the main controlling factors of shale oil occurrence under different lamina combinations.The differential enrichment mechanisms and patterns of shale oil were discussed using the shale oil micro-migration characterization and evaluation methods from the perspectives of relay hydrocarbon supply,stepwise migration,and multi-stage differentiation.The results are obtained in five aspects.First,Chang 73 shale mainly develops five types of lamina combination,i.e.non-laminated shale,sandy laminated shale,tuffaceous laminated shale,mixed laminated shale,and organic-rich laminated shale.Second,shales with different lamina combinations are obviously different in the reservoir space.Specifically,shales with sandy laminae and tuffaceous laminae have a large number of intergranular pores,dissolution pores and hydrocarbon generation-induced fractures.The multi-scale pore and fracture system constitutes the main place for liquid hydrocarbon occurrence.Third,the occurrence and distribution of shale oil in shale with different lamina combinations are jointly controlled by organic matter abundance,reservoir property,thermal evolution degree,mineral composition and laminae scale.The micro-nano-scale pore-fracture networks within shales containing rigid laminae,particularly sandy and tuffaceous laminations,primarily contain free-state light hydrocarbon components.In contrast,adsorption-phase heavy hydrocarbon components predominantly occupy surfaces of organic matter assemblages,clay mineral matrices,and framework mineral particulates.Fourth,there is obvious shale oil micro-migration between shales with different lamina combinations in Chang 73.Generally,such micro-migration is stepwise in a sequence of organic-rich laminated shale→tuffaceous laminated shale→mixed laminated shale→sandy lamiated shale→non-laminated shale.Fifth,the relay hydrocarbon supply of organic matter under the control of the spatial superposition of shales with various laminae,the stepwise migration via multi-scale pore and fracture network,and the multi-differentiation in shales with different lamina combinations under the control of organic-inorganic interactions fundamentally decide the differences of shale oil components between shales with different lamina combinations.
