Protein engineering involves modifying proteins'sequences to achieve desired functions,such as enhanced stability,improved activity,altered specificity,reduced immunogenicity,and improved pharmacokinetics.Rational...Protein engineering involves modifying proteins'sequences to achieve desired functions,such as enhanced stability,improved activity,altered specificity,reduced immunogenicity,and improved pharmacokinetics.Rational design and directed evolution(Arnold,1996)are two major traditional approaches for protein engineering.Rational design uses the knowledge of protein structure-function relationships to intentionally introduce specific mutations,while directed evolution mimics natural selection by generating random mutations and screening for desired protein functions.展开更多
文摘Protein engineering involves modifying proteins'sequences to achieve desired functions,such as enhanced stability,improved activity,altered specificity,reduced immunogenicity,and improved pharmacokinetics.Rational design and directed evolution(Arnold,1996)are two major traditional approaches for protein engineering.Rational design uses the knowledge of protein structure-function relationships to intentionally introduce specific mutations,while directed evolution mimics natural selection by generating random mutations and screening for desired protein functions.
