Graphical abstract 1.Multidisciplinary strategies to enhance therapeutic effects of flavonoids from Epimedi Folium:Integration of herbal medicine,enzyme engineering,and nanotechnology Yi Lu,QiulanLuo,Xiaobin Jia,James...Graphical abstract 1.Multidisciplinary strategies to enhance therapeutic effects of flavonoids from Epimedi Folium:Integration of herbal medicine,enzyme engineering,and nanotechnology Yi Lu,QiulanLuo,Xiaobin Jia,James P.Tam,Huan Yang.展开更多
Biopolymer core-shell microspheres play a crucial role in various biomedical applications,including drug delivery,tissue engineering,and diagnostics.These applications require microparticles with consistent,well-contr...Biopolymer core-shell microspheres play a crucial role in various biomedical applications,including drug delivery,tissue engineering,and diagnostics.These applications require microparticles with consistent,well-controlled size and precise shape fidelity.However,achieving high-throughput synthesis of size and shape-controlled core-shell biopolymer microgels remains a significant challenge.Herein,we present a one-step process for the high-throughput generation of monodisperse,luminescent,chitosan alginate core-shell microspheres by a novel manipulation of a centrifugal microfluidic device.We utilized the pH sensitivity of chitosan and the ionic gelation properties of alginate to create well-defined core-shell morphologies.To address particle merging issues and promote uniform particle size generation,we introduced an innovative pulsed mode operation in our centrifugal microfluidics device.We also incorporated fluorescent,nitrogen-functionalized graphene quantum dots into the core-shell structures,thereby rendering them useful for real-time imaging,which is necessary for diagnostic and therapeutic applications.To enhance biocompatibility,the alginate solution was supplemented with fish gelatin(FG).The resulting microspheres exhibited excellent structural integrity maintaining their core-shell structure after 15 days.Biocompatibility was demonstrated by C2C12 cell viability exceeding 88%after 15 days and by bacterial viability reaching the same percentage after 2 days.The system demonstrates considerable scalability,allowing for the consistent production of large quantities of microspheres without compromising functionality.The streamlined and efficient methodology simplifies the production process while unlocking new possibilities in targeted therapies,tissue regeneration,and diagnostics.展开更多
Research on cells and organ-like tissues is critical in the fields of molecular biology,genetic analysis,proteomics analysis,tissue engineering,and others.In recent years,advancements in precise cell manipulation tech...Research on cells and organ-like tissues is critical in the fields of molecular biology,genetic analysis,proteomics analysis,tissue engineering,and others.In recent years,advancements in precise cell manipulation technologies have made precise positioning and batch processing of cells feasible.Various methods are used for cell recognition,positioning,manipulation,and assembly,often introducing external fields such as electric,magnetic,acoustic,or optical fields into the liquid environment to interact with cells,applying forces to induce cell movement and rearrangement.Alternatively,three-dimensional(3D)bioprinting technology is employed for precise cell positioning and assembly.This review will comprehensively assess the status,principles,advantages,disadvantages,and prospects of these precise cell manipulation technologies,covering single-cell manipulation,multicellular assembly,and biological 3D printing techniques.展开更多
Stimuli-responsive polymers have the extraordinary ability to change their physical and/or chemical state after they‘‘detect’’a change in their environment;their response depends dramatically on their chemical com...Stimuli-responsive polymers have the extraordinary ability to change their physical and/or chemical state after they‘‘detect’’a change in their environment;their response depends dramatically on their chemical composition.This property has been used for a plethora of applications;this review highlights their utility for human health.Specifically,this review will highlight efforts in the areas of sensing and biosensing,antimicrobial/antifouling coatings,tissue engineering and regenerative medicine,and drug delivery.Specific examples are given in each of these areas,with some focus on our work engineering poly(Nisopropylacrylamide)-based microgels and other responsive systems.展开更多
文摘Graphical abstract 1.Multidisciplinary strategies to enhance therapeutic effects of flavonoids from Epimedi Folium:Integration of herbal medicine,enzyme engineering,and nanotechnology Yi Lu,QiulanLuo,Xiaobin Jia,James P.Tam,Huan Yang.
基金support of CONAHCYT(Consejo Nacional de Humanidades,Ciencias y Tecnologías,México)in the form of Graduate Program Scholarships and the support by Tecnológico de Monterrey in the form of tuition fee waiverthe funding provided by CONAHCYT in the form of scholarship as member of the National System of Researchers(SNI 1047863)+2 种基金he financial support of Federico Baur Endowed Chair in Nanotechnology(ILST002-23ID69001)the funding provided by CONAHCYT in the form of scholarship as a member of the National System of Researchers(CVU:969467)the financial support of FEMSA foundation.
文摘Biopolymer core-shell microspheres play a crucial role in various biomedical applications,including drug delivery,tissue engineering,and diagnostics.These applications require microparticles with consistent,well-controlled size and precise shape fidelity.However,achieving high-throughput synthesis of size and shape-controlled core-shell biopolymer microgels remains a significant challenge.Herein,we present a one-step process for the high-throughput generation of monodisperse,luminescent,chitosan alginate core-shell microspheres by a novel manipulation of a centrifugal microfluidic device.We utilized the pH sensitivity of chitosan and the ionic gelation properties of alginate to create well-defined core-shell morphologies.To address particle merging issues and promote uniform particle size generation,we introduced an innovative pulsed mode operation in our centrifugal microfluidics device.We also incorporated fluorescent,nitrogen-functionalized graphene quantum dots into the core-shell structures,thereby rendering them useful for real-time imaging,which is necessary for diagnostic and therapeutic applications.To enhance biocompatibility,the alginate solution was supplemented with fish gelatin(FG).The resulting microspheres exhibited excellent structural integrity maintaining their core-shell structure after 15 days.Biocompatibility was demonstrated by C2C12 cell viability exceeding 88%after 15 days and by bacterial viability reaching the same percentage after 2 days.The system demonstrates considerable scalability,allowing for the consistent production of large quantities of microspheres without compromising functionality.The streamlined and efficient methodology simplifies the production process while unlocking new possibilities in targeted therapies,tissue regeneration,and diagnostics.
基金National Natural Science Foundation of China,Grant/Award Numbers:52205312,52275200。
文摘Research on cells and organ-like tissues is critical in the fields of molecular biology,genetic analysis,proteomics analysis,tissue engineering,and others.In recent years,advancements in precise cell manipulation technologies have made precise positioning and batch processing of cells feasible.Various methods are used for cell recognition,positioning,manipulation,and assembly,often introducing external fields such as electric,magnetic,acoustic,or optical fields into the liquid environment to interact with cells,applying forces to induce cell movement and rearrangement.Alternatively,three-dimensional(3D)bioprinting technology is employed for precise cell positioning and assembly.This review will comprehensively assess the status,principles,advantages,disadvantages,and prospects of these precise cell manipulation technologies,covering single-cell manipulation,multicellular assembly,and biological 3D printing techniques.
基金Michael J.Serpe acknowledges funding from the University of Alberta(the Department of Chemistry and the Faculty of Science)the Natural Sciences and Engineering Research Council of Canada(NSERC)+1 种基金the Canada Foundation for Innovation(CFI)the Alberta Advanced Education&Technology Small Equipment Grants Program(AET/SEGP),IC-IMPACTS,and Grand Challenges Canada
文摘Stimuli-responsive polymers have the extraordinary ability to change their physical and/or chemical state after they‘‘detect’’a change in their environment;their response depends dramatically on their chemical composition.This property has been used for a plethora of applications;this review highlights their utility for human health.Specifically,this review will highlight efforts in the areas of sensing and biosensing,antimicrobial/antifouling coatings,tissue engineering and regenerative medicine,and drug delivery.Specific examples are given in each of these areas,with some focus on our work engineering poly(Nisopropylacrylamide)-based microgels and other responsive systems.
