Numerous studies have shown that intestinal and urinary tract flora are closely related to the formation of kidney stones.The removal of probiotics represented by lactic acid bacteria and the colonization of pathogeni...Numerous studies have shown that intestinal and urinary tract flora are closely related to the formation of kidney stones.The removal of probiotics represented by lactic acid bacteria and the colonization of pathogenic bacteria can directly or indirectly promote the occurrence of kidney stones.However,currently existing natural probiotics have limitations.Synthetic biology is an emerging discipline in which cells or living organisms are genetically designed and modified to have biological functions that meet human needs,or even create new biological systems,and has now become a research hotspot in various fields.Using synthetic biology approaches of microbial engineering and biological redesign to enable probiotic bacteria to acquire new phenotypes or heterologous protein expression capabilities is an important part of synthetic biology research.Synthetic biology modification of microorganisms in the gut and urinary tract can effectively inhibit the development of kidney stones by a range of means,including direct degradation of metabolites that promote stone production or indirect regulation of flora homeostasis.This article reviews the research status of engineered microorganisms in the prevention and treatment of kidney stones,to provide a new and effective idea for the prevention and treatment of kidney stones.展开更多
A azoreductase gene with 537 bp was obtained by PCR amplification from Rhodobacter sphaeroides AS1 1737 The enzyme, with a molecular weight of 18 7 kD, was efficiently expressed in Escherichia coli and its biodegr...A azoreductase gene with 537 bp was obtained by PCR amplification from Rhodobacter sphaeroides AS1 1737 The enzyme, with a molecular weight of 18 7 kD, was efficiently expressed in Escherichia coli and its biodegradation characteristics for azo dyes were investigated. Furthermore, the reaction kinetics and mechanism of azo dyes catalyzed by the genetically engineered azoreductase were studied in detail. The presence of a hydrazo-intermediate was identified, which provided a convincing evidence for the assumption that azo dyes were degraded via an incomplete reduction stage.展开更多
文摘Numerous studies have shown that intestinal and urinary tract flora are closely related to the formation of kidney stones.The removal of probiotics represented by lactic acid bacteria and the colonization of pathogenic bacteria can directly or indirectly promote the occurrence of kidney stones.However,currently existing natural probiotics have limitations.Synthetic biology is an emerging discipline in which cells or living organisms are genetically designed and modified to have biological functions that meet human needs,or even create new biological systems,and has now become a research hotspot in various fields.Using synthetic biology approaches of microbial engineering and biological redesign to enable probiotic bacteria to acquire new phenotypes or heterologous protein expression capabilities is an important part of synthetic biology research.Synthetic biology modification of microorganisms in the gut and urinary tract can effectively inhibit the development of kidney stones by a range of means,including direct degradation of metabolites that promote stone production or indirect regulation of flora homeostasis.This article reviews the research status of engineered microorganisms in the prevention and treatment of kidney stones,to provide a new and effective idea for the prevention and treatment of kidney stones.
文摘A azoreductase gene with 537 bp was obtained by PCR amplification from Rhodobacter sphaeroides AS1 1737 The enzyme, with a molecular weight of 18 7 kD, was efficiently expressed in Escherichia coli and its biodegradation characteristics for azo dyes were investigated. Furthermore, the reaction kinetics and mechanism of azo dyes catalyzed by the genetically engineered azoreductase were studied in detail. The presence of a hydrazo-intermediate was identified, which provided a convincing evidence for the assumption that azo dyes were degraded via an incomplete reduction stage.
