In this study,two wheat-derived cadmium(Cd)-immobilizing endophytic Pseudomonas paralactis M14 and Priestia megaterium R27 were evaluated for their effects on wheat tissue Cd uptake under hydroponic conditions.Then,th...In this study,two wheat-derived cadmium(Cd)-immobilizing endophytic Pseudomonas paralactis M14 and Priestia megaterium R27 were evaluated for their effects on wheat tissue Cd uptake under hydroponic conditions.Then,the impacts of the biochar(BC),M14+R27(MR),and BC+MR treatments on wheat Cd uptake and the mechanisms involved were investigated at the jointing,heading,and mature stages of wheat plants under field-plot conditions.A hydroponic experiment showed that the MR treatment significantly decreased the above-ground tissue Cd content compared with theM14 or R27 treatment.The BC+MRtreatment reduced the grain Cd content by 51.5%-67.7%and Cd translocation factor at the mature stage of wheat plants and increased the organic matter-bound Cd content by 31%-75%in the rhizosphere soils compared with the BC or MR treatment.Compared with the BC or MR treatment,the relative abundances of the biomarkers associated with Gemmatimonas,Altererythrobacter,Gammaproteobacteria,Xanthomonadaceae,Phenylobacterium,and Nocardioides in the BC+MR-treated rhizosphere microbiome decreased and negatively correlated with the organic matter-bound Cd contents.In the BC+MR-treated root interior microbiome,the relative abundance of the biomarker belonging to Exiguobacterium increased and negatively correlated with the Cd translocation factor,while the relative abundance of the biomarker belonging to Pseudonocardiaceae decreased and positively correlated with the Cd translocation factor.Our findings suggested that the BC+MR treatment reduced Cd availability and Cd transfer through affecting the abundances of these specific biomarkers in the rhizosphere soil and root interior microbiomes,leading to decreased wheat grain Cd uptake in the contaminated soil.展开更多
Oryza longistaminata is an African wild rice species with valuable agronomic traits and the donor parent of perennial rice.Endophytic bacteria play an important role in host health,adaptive evolution and stress tolera...Oryza longistaminata is an African wild rice species with valuable agronomic traits and the donor parent of perennial rice.Endophytic bacteria play an important role in host health,adaptive evolution and stress tolerance.However,endophytic bacterial communities in O.longistaminata and their plant growth-promoting(PGP)effects on the perennial rice of O.longistaminata offspring are poorly understood.In this study,the endophytic bacterial diversity,composition and network structures in the root,stem,and leaf tissues of O.longistaminata were characterized using Illumina sequencing of the 16S rRNA gene.The results suggested that O.longistaminata contains a multitude of niches for different endophytic bacteria,among which the root endosphere is more complex and functionally diverse than the stem and leaf endospheres.Tissue-specific biomarkers were identified,including Paludibaculum,Pseudactinotalea and Roseimarinus and others,for roots,Blautia for stems and Lachnospiraceae NK4A136 for leaves.The endophytic bacterial network of O.longistaminata was reassembled for various functions,including degradation/utilization/assimilation,detoxification,generation of precursor metabolites and energy,glycan pathways,macromolecule modification and metabolism.A total of 163 endophytic bacterial strains with PGP traits of potassium release,phosphate solubilization,nitrogen fixation,siderophore activity,indole-3-acetic acid(IAA)production,and 1-aminocyclopropane-1-carboxylate(ACC)deaminase activity were isolated from O.longistaminata.Eleven strains identified as Enterobacter cloacae,Enterobacter ludwigii,Stenotrophomonas maltophilia,Serratia fonticola,and Bacillus velezensis showed stable colonization abilities and PGP effects on perennial rice seedlings.Inoculated plants generally exhibited an enhanced root system and greater photosynthesis,biomass accumulation and nutrient uptake.Interestingly,two strains of E.cloacae have host genotype-dependent effects on perennial rice growth.The results of this study provide insights into the endophytic bacterial ecosystems of O.longistaminata,which can potentially be used as biofertilizers for sustainable perennial rice productivity.展开更多
A chemical investigation of Streptomyces sp.GZWMJZ-662,an endophytic actinomycete isolated from Houttuynia cordata Thunb.,has yielded eleven bohemamine dimers(1-11).Notably,the newly identified dibohemamines I-O(1-7)h...A chemical investigation of Streptomyces sp.GZWMJZ-662,an endophytic actinomycete isolated from Houttuynia cordata Thunb.,has yielded eleven bohemamine dimers(1-11).Notably,the newly identified dibohemamines I-O(1-7)have not been previously reported.Their structures were elucidated through detailed spectroscopic analysis,encompassing high-resolution electrospray ionization mass,nuclear magnetic resonance,infrared radiation,ultraviolet-visible,and electronic circular dichroism spectroscopy.Dibohemamine I(1)exhibited selective cytotoxic effects against the cancer cell lines 786-O and GBC-SD among the 18 cell lines evaluated,with the half-inhibitory concentration values of 3.24±0.20 and 7.36±0.41μM,respectively.展开更多
[Objectives]This study was conducted to explore the culture conditions for siderophore production and preliminarily investigate the growth-promoting effects of strains.[Methods]Endophytic bacteria secreting siderophor...[Objectives]This study was conducted to explore the culture conditions for siderophore production and preliminarily investigate the growth-promoting effects of strains.[Methods]Endophytic bacteria secreting siderophores were isolated and screened from the roots of the plant Saposhnikovia divaricata(Trucz.)Schischk.The siderophore-producing capability was confirmed through qualitative detection and gene cluster analysis.[Results]The screened endophytic bacterium PB-2 belonged to the genus Paenibacillus sp.and could produce catechol-type or carboxylate-type siderophores.When cultured in MKB medium with 15 g/L glycerol as the carbon source and 6 g/L glycine as the nitrogen source for 3 d,the strain exhibited efficient siderophore secretion.Appropriate concentrations of Fe^(3+),Co^(2+),Cu^(2+)and Al^(3+)promoted siderophore production,with 15μmol/L Fe^(3+)being the most effective.Under the combined influences of siderophores,volatile compounds and other factors,strain PB-2 altered the root morphology of mulberry seedlings and increased their biomass.The primary root length decreased by 9.15%,while the number and length of root hairs increased by 37.93%and 16.37%,respectively,and the total biomass increased by 10.26%,all showing significant differences(P<0.05).Additionally,strain PB-2 enhanced the activities of defense-related enzymes in mulberry seedling leaves.The activities of superoxide dismutase(SOD)and peroxidase(POD)increased significantly(P<0.05)by 247.27%and 189.47%,respectively,compared with the control group.The activities of phenylalanine ammonialyase(PAL)and polyphenol oxidase(PPO)also showed varying degrees of increase.[Conclusions]This study provided a theoretical basis for rational utilization of endophytic growth-promoting strains secreting siderophores in the sustainable development of agriculture.展开更多
Phthalic acid esters(PAEs)are widely released into the environment due to industrial and agricultural activities.This poses significant risks,not only to crops grown on contaminated soil but also to humans.PAEs enter ...Phthalic acid esters(PAEs)are widely released into the environment due to industrial and agricultural activities.This poses significant risks,not only to crops grown on contaminated soil but also to humans.PAEs enter the human body through the food chain,causing potential health hazards.Endophytic bacteria have demonstrated a significant potential as effective bioremediation agents,with specialized mechanisms of PAEs degradation.Endophytic bacteria such as Rhodococcus,Pseudomonas and Sphingomona recognize root exudates,adhere to and penetrate root surfaces,and ultimately colonize crops.They form symbiotic relationships with crops,obtaining nutrients and habitats from crops,meanwhile,promoting plant growth and health through hormone production,nutrient regulation,and the suppression of pathogenic microorganisms.Furthermore,endophytic bacteria efficiently degrade PAEs in soil-crop systems through synergistic interactions with indigenous rhizosphere microflora and regulatory effects on enzyme activity in crops.Here,we review the role of endophytic bacteria in the bioremediation of PAEs-contaminated crops and soils.In addition,we discuss the scarcity of endophytic bacterial strains with a confirmed ability to degrade PAEs,and underscore the lack of the research on the mechanisms of PAEs degradation by these bacteria.This review also points out that future study should investigate the molecular mechanisms underlying the interaction between endophytic bacteria and PAEs to offer novel insights and solutions for environmental protection and sustainable agricultural development.展开更多
[Objectives]To investigate the mechanism of endophytic fungi mediating the plant growth and promoting the accumulation of secondary metabolites in Camellia oleifera.[Methods]Four strains of endophytic fungi isolated f...[Objectives]To investigate the mechanism of endophytic fungi mediating the plant growth and promoting the accumulation of secondary metabolites in Camellia oleifera.[Methods]Four strains of endophytic fungi isolated from the rhizomes of C.oleifera were co-cultured with C.oleifera seedlings individually in sterile soil for 49 d:Didymella sp.(DS),Fusarium sp.(FS),Penicillium sp.(PS),and Clonostachys rosea(CR).[Results]The biological activities of the four fungal strains differed,but all exhibited the ability to promote quercetin accumulation while simultaneously reducing quercetin glycosides after co-culture with C.oleifera seedlings.The DS,FS and PS treatments resulted in a significant increase in the leaf area of C.oleifera,with all of the experimental groups exhibiting a weight increase of over 50%compared to the control(CON)group.[Conclusions]Our findings demonstrate the potential utility of endophytic fungi in the production of C.oleifera,highlighting their capacity to enhance both productivity and the accumulation of plant metabolites.展开更多
Three bacterial endophytes of Sedum alfredii, VI8L2, II8L4 and VI8R2, were examined for promoting soil Zn bioavailability and Zn accumulation in S. alfredii. Results showed that three strains were re-introduced into S...Three bacterial endophytes of Sedum alfredii, VI8L2, II8L4 and VI8R2, were examined for promoting soil Zn bioavailability and Zn accumulation in S. alfredii. Results showed that three strains were re-introduced into S. alfredii rhizosphere soils under Zn stress and resulted in better plant growth, as roots biomass increased from 80% to 525% and shoot biomass from 11% to 47% compared with the uninoculated ones. Strains IVsLz, II8L4 and IVsR2 significantly increased shoot and root Zn concentrations in the ZnCO3 contaminated soil. Inoculation with strain IVsL2 resulted in 44% and 39% higher shoot and root Zn concentrations, while strain IV8R2 significantly decreased shoot Zn concentration in the Zn3（PO4）2 contaminated soils. In the aged contaminated soil, isolates IVsL2, IIsL4 and IVsR2 significantly increased root Zn concentration, but decreased shoot Zn concentration of Sedum alfredii. It suggested that endophytes might be used for enhancing phytoextraction efficiency.展开更多
Three known compounds were isolated from the endophytic fungus Trichoderma sp Xy24 from a mangrove plant Xylocarpus granatum by using various column chromatography techniques. Their structures were identified as harzi...Three known compounds were isolated from the endophytic fungus Trichoderma sp Xy24 from a mangrove plant Xylocarpus granatum by using various column chromatography techniques. Their structures were identified as harzianone （1）, trichoacorenol （2）, and trichodimerol （3） by extensive spectroscopic analysis. Among them, 1 was a harziane diterpene, 2 was a sesquiterpene alcohol, and 3 was a polyketide with a completely symmetric configuration. Compound 3 exhibited medium inhibitory activity with an IC50 value of 74.6 μM using a NA （H7N9）/MUNANA model.展开更多
[Objective] The study aimed to investigate the growth-promoting activities of endophytic bacteria from tomato plants.[Method]The endophytic bacteria isolated from different tissues of tomato plants were analyzed for t...[Objective] The study aimed to investigate the growth-promoting activities of endophytic bacteria from tomato plants.[Method]The endophytic bacteria isolated from different tissues of tomato plants were analyzed for the effects of their growth-promoting activities on the germination and growth of tomato plants.The bacteria with growth-promoting activity were preliminarily identified.[Result]Totally 59 endophytic bacterial strains were isolated from roots and stems of tomatoes,of which 4 showed significantly growth-promoting activity to germination and growth of tomato.The results suggest that these strains are endowed with the potential capability of growth-promoting.[Conclusion]The endophytic bacteria with growth-promoting activity were found among the isolates from tomato plants.This provided a good foundation for utilization of these bacteria with growth-promoting activity.展开更多
[Objective] With a strain of endophytic antagonistic bacteria with good disease control efficacy in pot as trial material,the paper was to explore the antagonistic mechanism of siderophore against Fusarium oxysporum.[...[Objective] With a strain of endophytic antagonistic bacteria with good disease control efficacy in pot as trial material,the paper was to explore the antagonistic mechanism of siderophore against Fusarium oxysporum.[Method] Whether the strain produced siderophore and its fluorescent property was judged by MSA（Modified Sugar-Aspartic acid） plate.The siderophore activity of strains in liquid MSA medium was determined through the absorbance values at particular wavelength.The inhibition effects of it siderophore against F.oxysporum under different concentrations of Fe3＋ were compared.Then the strain was preliminarily identified based on morphological,physiological and biochemical characteristics [Result] The fluorescent siderophore produced by the endophytic bacteria in MSA medium had a strong inhibition effect against F.oxysporum.With the increasing concentration of Fe3＋,the inhibition effect against the pathogen weakened.The preliminary analysis showed these strains belonged to Bacillus.[Conclusion] Bacillus could compete the absorption of Fe3＋ by secreting siderophore to inhibit the growth of F.oxysporum.展开更多
[Objective] The aim was to isolate and identify a taxol-producing endophytic fungus from Taxus media. [Method] 32 strains of endophytic fungi were identified form the inner bark of T. media,and their fermentation prod...[Objective] The aim was to isolate and identify a taxol-producing endophytic fungus from Taxus media. [Method] 32 strains of endophytic fungi were identified form the inner bark of T. media,and their fermentation products were detected by high performance liquid chromatography (HPLC). [Result] Through the screening,a strain of taxol-producing endophytic fungi M57 was obtained,which could produce 45-50 μg/L of taxol,and M57 was defined as Rhizopus sp. through morphological observation and 18S rDNA sequence analysis. [Conclusion] The finding of Rhizopus sp. M57 provided a promising strain for producing taxol with taxol-producing fungi fermentation process.展开更多
[ Objective ] The paper was to study the growth-promoting and antagonistic action of endophytic bacteria strains Itb57 and Itb295 of tobacco to explore their functions in biological control. [ Method] The growth-promo...[ Objective ] The paper was to study the growth-promoting and antagonistic action of endophytic bacteria strains Itb57 and Itb295 of tobacco to explore their functions in biological control. [ Method] The growth-promoting effects of bacterial suspension ~ff endophytic bacteria Itb57 and Itb295 on tobacco seedling un- der different treatment modes were studied using potting method in greenhouse. The antagonistic action of bacterial suspension of endophytic bacteria Itb57 and Itb295 on Phytophthora nicotianae, Alternaria alternata and Botrytis cinerea were measured by duel culture method. [ Result] Bacterial suspensions of enduphytic bacteria Itb57 and Itb295 had certain growth-promoting effects on tobacco seedling, which could significantly increase the fresh weight and dry weight in aerial part; the growth-promoting effect of soaking ＋ spraying and irrigating treatment was the best. Itb57 strain had good antagonistic action against P. nicotianae. A. alterna- ta and B. cinerea, while Itb295 strain only had good antifungal effect against P. nicotianae. [ Conclusion] The results provided basis for the study and application of tobacco endophytic bacteria strains Itb57 and Itb295 in biocontrol of tobacco diseases.展开更多
[Objective] This study aimed to investigate the feasibility of agricultural irri- gation with reclaimed water in Urumqi City. [Method] Chinese cabbages were used as experimental materials and irrigated with control wa...[Objective] This study aimed to investigate the feasibility of agricultural irri- gation with reclaimed water in Urumqi City. [Method] Chinese cabbages were used as experimental materials and irrigated with control water, 50% reclaimed water and 100% reclaimed water, and then the number of endophytic bacteria in Chinese cab- bages was measured. [Result] Using 50% reclaimed water, the fresh weight of Chi- nese cabbage was improved by 68.94%; however, with the deepening internalization of exogenous microorganism internalization, the total number of endophytic bacteria and coliform flock in Chinese cabbages treated with 50% reclaimed water was sig- nificantly higher than the other two treatment groups （P〈0.05）. [Conclusion] Re- claimed water enhances both the weight of Chinese cabbage and the number of endophytic bacteria, which is unsuitable for irrigation of edible crops such as Chi- nese cabbage. Strengthening disinfection during the process of sewerage treatment could solve this problem.展开更多
[Objective] This study aimed to isolate and screen black-spot-resistant en- dophytic fungi strains. [Method] Various species of healthy Rosa chinensis were col- lected from Xi＇an, Xianyang, Baoji and Weinan City in S...[Objective] This study aimed to isolate and screen black-spot-resistant en- dophytic fungi strains. [Method] Various species of healthy Rosa chinensis were col- lected from Xi＇an, Xianyang, Baoji and Weinan City in Shaanxi Province. Endophytic fungi was isolated from stems and leaves, and purified by 2-3 times of inoculation to screen endophytic fungi antagonistic to Marssonina rosae with modified punching method. [Result] Samples collected from Xianyang exhibited the highest colonization rate and isolation rate; endophytic fungi strains isolated from stems presented the highest colonization rate and isolation rate compared with leaves. A total of 67 en- dophytic fungi strains were isolated from Rosa chinensis, including 3 black spot-re- sistant strains which were all derived from Baoji. [Conclusion] This study laid the foundation for further screening candidate strains of biocontrol fungi and environ- ment-friendly fungal biological control.展开更多
[ Objective ] The paper was to study and analyze the genetic diversity of 48 endophytic bacteria isolated from Sophora alopecuroide with strong antagonis- tic effect against Fusarium oxysporum and Verticillium dahliae...[ Objective ] The paper was to study and analyze the genetic diversity of 48 endophytic bacteria isolated from Sophora alopecuroide with strong antagonis- tic effect against Fusarium oxysporum and Verticillium dahliae, and carry out sequence measurement and phylogenetic analysis on 10 representative strains. [ Meth- od] Using total DNA ERIC-PCR and 16S rRNA sequencing analysis method, a total of 48 endophytic bacteria were carried out genetic diversity and phylogenetic analysis. [Result] ERIC-PCR fingerprint map showed that 48 endophytic bacteria were divided into 6 ERIC groups and 2 strains with independent groups at Wat- son distance of 0.31. Representative strains were selected from each group to determine 16S rRNA gene for phylogenetic analysis, and the results showed that these strains belonged to Bacillus atrophaeus, Bacillus subtilis, Bacillus cereus, Bacillus megaterium, Bacillus pumilus and Serratia marcescens, respectively. [ Conclu- sion ] The genetic diversity of 48 strains of bacteria was obvious. Determination of 48 strains of bacteria on diversity and phylogenetic status could lay foundation for study on mechanism of F. oxysporum and V. dahliae, which could also provide new strain sources for biological control of diseases in cotton production.展开更多
[Objective] The aim was to separate and purify antioxidant endophytic fun- gi from Stevia leaves. [Method] Stevia leaves were used as materials for endophytic fungi screening and purification. Then, liquid culture was...[Objective] The aim was to separate and purify antioxidant endophytic fun- gi from Stevia leaves. [Method] Stevia leaves were used as materials for endophytic fungi screening and purification. Then, liquid culture was conducted to test fermented supernatant abilities of antioxidant, DPPH free radical clearing and chemilumines- cence inhibition. [Result] Forty two endophytic fungi were separated from F01 to F42. F41 was proved to be the strongest in antioxidant aspect; F37 was the strongest in DPPH clearing; F02, F07, F20, F25, F37, and F416 stood out in a comprehensive comparison of the above aspects. Flow injection chemiluminescenct method was used to test the above six fermentation liquors, and the results showed that antioxidant abilities of the six were quite strong for all of chemiluminescence rates were over 70%. Through preliminary morphological identification, bacterial colony was found white or yellowy and only F02 was of dissepiment. [Conclusion] Fungi with high-antioxidation activities were separated, which paved the way for future research.展开更多
[Objective] The study was aimed to provide theoretical foundation for developing a new fungicide with high activity,low toxicity and low residua.[Method] Based on the plant growth rate and inhibition zone methods,the ...[Objective] The study was aimed to provide theoretical foundation for developing a new fungicide with high activity,low toxicity and low residua.[Method] Based on the plant growth rate and inhibition zone methods,the antifungal activity of extracts of every organ of Xanthium sibiricum,the fermentation liquid of endophytic fungi MC4-23,and the extract of mycelium were tested with six kinds of pathogenic fungi and three kinds of pathogenic bacteria respectively.[Result] The extract of every organ of Xanthium sibiricum,the fermentation liquid of endophyic fungi MC4-23,and the extract of mycelium all had antifungal activities.The acetone extracts of Xanthium sibiricum had stronger antifungal activities than the ethanol extracts of Xanthium sibiricum.The extracts of leaf have the strongest antifungal activities against fungi and bacteria.The inhibition rates of the six pathogenic fungi were all above 47.8%,and the inhibition diameters of the three pathogenic bacteria were all above 0.5 centimeter.The fermentation liquid of endophytic fungi also had strong activities against the fungi and bacteria.The inhibition rates of the six pathogenic fungi were all above 68.1%.The extracts of root of Xanthium sibiricum and the mycelium of endophytic fungi were all had antifungal activities.[Conclusion] Xanthium sibiricum and endophyic fungi MC4-23 had great value of development and utilization.展开更多
A total of 94 isolates of endophytic fungi were isolated from the bark of 200-yr.-old Taxus cuspidata Sieb. et Zucc. in the primeval forest of the Changbai Mountain Natural Reserve, and 19 species of endophytic fungi ...A total of 94 isolates of endophytic fungi were isolated from the bark of 200-yr.-old Taxus cuspidata Sieb. et Zucc. in the primeval forest of the Changbai Mountain Natural Reserve, and 19 species of endophytic fungi were identified. Among the taxa 2 new species and 1 new varied species as well as the 6 known species of China had been described in a previous article published on Journal of Forestry Research, Vol. 14 (4): 290-294 (2003). This paper made a detail description on the morpho-logical characteristics of the 9 new recorded-genus-species and 1 new record species with illustrations, including Staphy-lotrichum coccosporum Meyer et Nicot, Botryodema lateritium Papendoorf & Upadhyay, Oedocephalum glomerulosum (Rul-liard) Sacc., Trichosporonoides oedocephalis Haskins & Spencer, Beniowskia sphaeroidea (Kal Chbrenner et Caeke) Mason, Thermomyces verrucosus Pugh, Blakeman & Morgan-jones, Gilmaniella humicola Brron , Arthrinium sphaerosperma (Per.:Fr.) Ellis, Ambrosiella brunnea (Verrall) Batra, and Cytosporina notha Diedz (1 new record species). And the habitats, hosts, distribu-tions and fruiting periods were recorded for the new recorded-genus-species. The determination result of thin layer chroma-tography (TLC) showed that the liquid fermentation metabolites of the strains could not react positively with taxoids developer - the vanillic aldehyde and dilute bismuth potassium iodide.展开更多
[Objective] This study aimed to isolate the endophytic fungi of Paris polyphylla var. yunnanensis and investigate their effects on the embryo development of P. polyphylla var. yunnanensis seeds. [Method] The endophyti...[Objective] This study aimed to isolate the endophytic fungi of Paris polyphylla var. yunnanensis and investigate their effects on the embryo development of P. polyphylla var. yunnanensis seeds. [Method] The endophytic fungi of P. polyphylla were isolated and identified morphologically, and their effects on the embryo development of P. polyphylla var. yunnanensis seeds were studied by using paraffin sectioning and microphotography. [Result] Nine endophytic fungi, i.e. P. polyphylla var. yunnanensis endophytic fungi PPYEF-1, PPYEF-2, PPYEF-3, PPYEF-4, PPYEF-5, PPYEF-6, PPYEF-7, PPYEF-8 and PPYEF-9 belonging to seven genera in five families, three orders were isolated from the rhizomes. Except PPYEF-4 (Cladosporium sp.), other fungi could promote the embryo development of the P. polyphylla var. yunnanensis seeds, mostly reaching the extremely significant or significant level. PPYEF-9 (Trichoderma sp.) resulted in the highest embryo length and embryo-emerging ratio. [Conclusion] This paper could provide a reference for the application of the endophytic fungi of P. polyphylla var. yunnanensis in the dormancy-breaking of P. polyphylla var. yunnanensis seeds.展开更多
[Objective] To examine the effect of endophytic fungi ALl2 （Gilmaniella sp.） on metabolites distribution in organs of Atractylodes lancea. [Method] Endophytic fungi ALl2 was inoculated on Atraetylodes lancea plantle...[Objective] To examine the effect of endophytic fungi ALl2 （Gilmaniella sp.） on metabolites distribution in organs of Atractylodes lancea. [Method] Endophytic fungi ALl2 was inoculated on Atraetylodes lancea plantlets in tissue culture, and the distribution of cellulose, hemicellulose, lignin, soluble sugar in leaves and roots of the inoculated group were detected. The weight of leaves and roots were compared. Gas Chromatography was used to analyze the volatile oil components. [Result] Compared with the control group, the average fresh weight and dry weight of leaves and roots of A. lancea which had been symbiosed with ALl2 increased significantly. The content of lignin and soluble sugar increased in the leaves of the inoculated group, and the content of cellulose, hemicellulose, lignin, soluble sugar and volatile oil also increased in roots. [Conclusion] The results indicate that symbiosed with ALl2 is benefit for the development of A. lancea roots and can promote the transfer and accumulation of the medicinal components to the roots.展开更多
基金supported by the National Natural Science Foundation of China(No.41977199).
文摘In this study,two wheat-derived cadmium(Cd)-immobilizing endophytic Pseudomonas paralactis M14 and Priestia megaterium R27 were evaluated for their effects on wheat tissue Cd uptake under hydroponic conditions.Then,the impacts of the biochar(BC),M14+R27(MR),and BC+MR treatments on wheat Cd uptake and the mechanisms involved were investigated at the jointing,heading,and mature stages of wheat plants under field-plot conditions.A hydroponic experiment showed that the MR treatment significantly decreased the above-ground tissue Cd content compared with theM14 or R27 treatment.The BC+MRtreatment reduced the grain Cd content by 51.5%-67.7%and Cd translocation factor at the mature stage of wheat plants and increased the organic matter-bound Cd content by 31%-75%in the rhizosphere soils compared with the BC or MR treatment.Compared with the BC or MR treatment,the relative abundances of the biomarkers associated with Gemmatimonas,Altererythrobacter,Gammaproteobacteria,Xanthomonadaceae,Phenylobacterium,and Nocardioides in the BC+MR-treated rhizosphere microbiome decreased and negatively correlated with the organic matter-bound Cd contents.In the BC+MR-treated root interior microbiome,the relative abundance of the biomarker belonging to Exiguobacterium increased and negatively correlated with the Cd translocation factor,while the relative abundance of the biomarker belonging to Pseudonocardiaceae decreased and positively correlated with the Cd translocation factor.Our findings suggested that the BC+MR treatment reduced Cd availability and Cd transfer through affecting the abundances of these specific biomarkers in the rhizosphere soil and root interior microbiomes,leading to decreased wheat grain Cd uptake in the contaminated soil.
基金supported by funding from the National Natural Science Foundation of China(32060593 and 32060474)the Yunnan Provincial Science and Technology Department+4 种基金China(202101AT070021 and 202101AS070001)the Yunnan Provincial Department of Education Science Research Fund ProjectChina(2023J0006)the Graduate Innovation Project of Yunnan UniversityChina(KC-22223012 and ZC-22222760)。
文摘Oryza longistaminata is an African wild rice species with valuable agronomic traits and the donor parent of perennial rice.Endophytic bacteria play an important role in host health,adaptive evolution and stress tolerance.However,endophytic bacterial communities in O.longistaminata and their plant growth-promoting(PGP)effects on the perennial rice of O.longistaminata offspring are poorly understood.In this study,the endophytic bacterial diversity,composition and network structures in the root,stem,and leaf tissues of O.longistaminata were characterized using Illumina sequencing of the 16S rRNA gene.The results suggested that O.longistaminata contains a multitude of niches for different endophytic bacteria,among which the root endosphere is more complex and functionally diverse than the stem and leaf endospheres.Tissue-specific biomarkers were identified,including Paludibaculum,Pseudactinotalea and Roseimarinus and others,for roots,Blautia for stems and Lachnospiraceae NK4A136 for leaves.The endophytic bacterial network of O.longistaminata was reassembled for various functions,including degradation/utilization/assimilation,detoxification,generation of precursor metabolites and energy,glycan pathways,macromolecule modification and metabolism.A total of 163 endophytic bacterial strains with PGP traits of potassium release,phosphate solubilization,nitrogen fixation,siderophore activity,indole-3-acetic acid(IAA)production,and 1-aminocyclopropane-1-carboxylate(ACC)deaminase activity were isolated from O.longistaminata.Eleven strains identified as Enterobacter cloacae,Enterobacter ludwigii,Stenotrophomonas maltophilia,Serratia fonticola,and Bacillus velezensis showed stable colonization abilities and PGP effects on perennial rice seedlings.Inoculated plants generally exhibited an enhanced root system and greater photosynthesis,biomass accumulation and nutrient uptake.Interestingly,two strains of E.cloacae have host genotype-dependent effects on perennial rice growth.The results of this study provide insights into the endophytic bacterial ecosystems of O.longistaminata,which can potentially be used as biofertilizers for sustainable perennial rice productivity.
基金The National Natural Science Foundation of China,82460684,Li-Ping WangWest Light Foundation,Chinese Academy of Sciences,RZ[2022]4,Li-Ping Wang+3 种基金Research Foundation for Advanced Talents(D.Wang),TCZJZ[2022]02,Dong-Yang WangProject of State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University,QJJ[2022]419,Peng FuCultivation project of National Natural Science Foundation of Guizhou Medical University,20NSP065,Li-Ping Wangthe 100 Leading Talents of Guizhou Province,W.Zhu,Wei-Ming Zhu.
文摘A chemical investigation of Streptomyces sp.GZWMJZ-662,an endophytic actinomycete isolated from Houttuynia cordata Thunb.,has yielded eleven bohemamine dimers(1-11).Notably,the newly identified dibohemamines I-O(1-7)have not been previously reported.Their structures were elucidated through detailed spectroscopic analysis,encompassing high-resolution electrospray ionization mass,nuclear magnetic resonance,infrared radiation,ultraviolet-visible,and electronic circular dichroism spectroscopy.Dibohemamine I(1)exhibited selective cytotoxic effects against the cancer cell lines 786-O and GBC-SD among the 18 cell lines evaluated,with the half-inhibitory concentration values of 3.24±0.20 and 7.36±0.41μM,respectively.
基金Supported by Doctoral Start-up Fund of Jiangsu University of Science and Technology(1102931901).
文摘[Objectives]This study was conducted to explore the culture conditions for siderophore production and preliminarily investigate the growth-promoting effects of strains.[Methods]Endophytic bacteria secreting siderophores were isolated and screened from the roots of the plant Saposhnikovia divaricata(Trucz.)Schischk.The siderophore-producing capability was confirmed through qualitative detection and gene cluster analysis.[Results]The screened endophytic bacterium PB-2 belonged to the genus Paenibacillus sp.and could produce catechol-type or carboxylate-type siderophores.When cultured in MKB medium with 15 g/L glycerol as the carbon source and 6 g/L glycine as the nitrogen source for 3 d,the strain exhibited efficient siderophore secretion.Appropriate concentrations of Fe^(3+),Co^(2+),Cu^(2+)and Al^(3+)promoted siderophore production,with 15μmol/L Fe^(3+)being the most effective.Under the combined influences of siderophores,volatile compounds and other factors,strain PB-2 altered the root morphology of mulberry seedlings and increased their biomass.The primary root length decreased by 9.15%,while the number and length of root hairs increased by 37.93%and 16.37%,respectively,and the total biomass increased by 10.26%,all showing significant differences(P<0.05).Additionally,strain PB-2 enhanced the activities of defense-related enzymes in mulberry seedling leaves.The activities of superoxide dismutase(SOD)and peroxidase(POD)increased significantly(P<0.05)by 247.27%and 189.47%,respectively,compared with the control group.The activities of phenylalanine ammonialyase(PAL)and polyphenol oxidase(PPO)also showed varying degrees of increase.[Conclusions]This study provided a theoretical basis for rational utilization of endophytic growth-promoting strains secreting siderophores in the sustainable development of agriculture.
基金supported by the National Natural Science Foundation of China(No.22161132011)Jiangsu Provincial Special Fund for S&T Innovation in Carbon Emission Peak and Neutrality(No.20220013)+1 种基金the National Key Research and Development Program of China(Nos.2023YFE0110800 and 2023YFC3708100)the Fundamental Research Funds for the Central Universities(No.QTPY2024001).
文摘Phthalic acid esters(PAEs)are widely released into the environment due to industrial and agricultural activities.This poses significant risks,not only to crops grown on contaminated soil but also to humans.PAEs enter the human body through the food chain,causing potential health hazards.Endophytic bacteria have demonstrated a significant potential as effective bioremediation agents,with specialized mechanisms of PAEs degradation.Endophytic bacteria such as Rhodococcus,Pseudomonas and Sphingomona recognize root exudates,adhere to and penetrate root surfaces,and ultimately colonize crops.They form symbiotic relationships with crops,obtaining nutrients and habitats from crops,meanwhile,promoting plant growth and health through hormone production,nutrient regulation,and the suppression of pathogenic microorganisms.Furthermore,endophytic bacteria efficiently degrade PAEs in soil-crop systems through synergistic interactions with indigenous rhizosphere microflora and regulatory effects on enzyme activity in crops.Here,we review the role of endophytic bacteria in the bioremediation of PAEs-contaminated crops and soils.In addition,we discuss the scarcity of endophytic bacterial strains with a confirmed ability to degrade PAEs,and underscore the lack of the research on the mechanisms of PAEs degradation by these bacteria.This review also points out that future study should investigate the molecular mechanisms underlying the interaction between endophytic bacteria and PAEs to offer novel insights and solutions for environmental protection and sustainable agricultural development.
基金Supported by the Key Field Project of Guizhou Provincial Education Department(KY[2021]044)Guizhou Forestry Science Research Project(QJH KY[2021]11)Guizhou Higher Education Characteristic Key Laboratory Construction Project(QJH KY[2021]002).
文摘[Objectives]To investigate the mechanism of endophytic fungi mediating the plant growth and promoting the accumulation of secondary metabolites in Camellia oleifera.[Methods]Four strains of endophytic fungi isolated from the rhizomes of C.oleifera were co-cultured with C.oleifera seedlings individually in sterile soil for 49 d:Didymella sp.(DS),Fusarium sp.(FS),Penicillium sp.(PS),and Clonostachys rosea(CR).[Results]The biological activities of the four fungal strains differed,but all exhibited the ability to promote quercetin accumulation while simultaneously reducing quercetin glycosides after co-culture with C.oleifera seedlings.The DS,FS and PS treatments resulted in a significant increase in the leaf area of C.oleifera,with all of the experimental groups exhibiting a weight increase of over 50%compared to the control(CON)group.[Conclusions]Our findings demonstrate the potential utility of endophytic fungi in the production of C.oleifera,highlighting their capacity to enhance both productivity and the accumulation of plant metabolites.
基金Project(40973055) supported by the National Natural Science Foundation of ChinaProject(U0833004) supported by the NSFC-Guangdong Joint Foundation of China
文摘Three bacterial endophytes of Sedum alfredii, VI8L2, II8L4 and VI8R2, were examined for promoting soil Zn bioavailability and Zn accumulation in S. alfredii. Results showed that three strains were re-introduced into S. alfredii rhizosphere soils under Zn stress and resulted in better plant growth, as roots biomass increased from 80% to 525% and shoot biomass from 11% to 47% compared with the uninoculated ones. Strains IVsLz, II8L4 and IVsR2 significantly increased shoot and root Zn concentrations in the ZnCO3 contaminated soil. Inoculation with strain IVsL2 resulted in 44% and 39% higher shoot and root Zn concentrations, while strain IV8R2 significantly decreased shoot Zn concentration in the Zn3（PO4）2 contaminated soils. In the aged contaminated soil, isolates IVsL2, IIsL4 and IVsR2 significantly increased root Zn concentration, but decreased shoot Zn concentration of Sedum alfredii. It suggested that endophytes might be used for enhancing phytoextraction efficiency.
基金Science&Technology Project of Guangdong Province(Grant No.2011A080403020)the Fundamental Research Funds for the Central Universities(Grant No.2012N06)
文摘Three known compounds were isolated from the endophytic fungus Trichoderma sp Xy24 from a mangrove plant Xylocarpus granatum by using various column chromatography techniques. Their structures were identified as harzianone （1）, trichoacorenol （2）, and trichodimerol （3） by extensive spectroscopic analysis. Among them, 1 was a harziane diterpene, 2 was a sesquiterpene alcohol, and 3 was a polyketide with a completely symmetric configuration. Compound 3 exhibited medium inhibitory activity with an IC50 value of 74.6 μM using a NA （H7N9）/MUNANA model.
基金Supported by the Science Foundation for the Introduced High-level Talents in Anhui Agricultural University(yj2008-1)~~
文摘[Objective] The study aimed to investigate the growth-promoting activities of endophytic bacteria from tomato plants.[Method]The endophytic bacteria isolated from different tissues of tomato plants were analyzed for the effects of their growth-promoting activities on the germination and growth of tomato plants.The bacteria with growth-promoting activity were preliminarily identified.[Result]Totally 59 endophytic bacterial strains were isolated from roots and stems of tomatoes,of which 4 showed significantly growth-promoting activity to germination and growth of tomato.The results suggest that these strains are endowed with the potential capability of growth-promoting.[Conclusion]The endophytic bacteria with growth-promoting activity were found among the isolates from tomato plants.This provided a good foundation for utilization of these bacteria with growth-promoting activity.
基金Supported by National Natural Science Foundation of China(30960010 )Principal Fund Key Projects of Tarim University(TDZKZD06001)~~
文摘[Objective] With a strain of endophytic antagonistic bacteria with good disease control efficacy in pot as trial material,the paper was to explore the antagonistic mechanism of siderophore against Fusarium oxysporum.[Method] Whether the strain produced siderophore and its fluorescent property was judged by MSA（Modified Sugar-Aspartic acid） plate.The siderophore activity of strains in liquid MSA medium was determined through the absorbance values at particular wavelength.The inhibition effects of it siderophore against F.oxysporum under different concentrations of Fe3＋ were compared.Then the strain was preliminarily identified based on morphological,physiological and biochemical characteristics [Result] The fluorescent siderophore produced by the endophytic bacteria in MSA medium had a strong inhibition effect against F.oxysporum.With the increasing concentration of Fe3＋,the inhibition effect against the pathogen weakened.The preliminary analysis showed these strains belonged to Bacillus.[Conclusion] Bacillus could compete the absorption of Fe3＋ by secreting siderophore to inhibit the growth of F.oxysporum.
基金Supported by National Natural Science Foundation of China(20776058)New Century Training Programme Foundation for the Talents by the State Education Commission (NCET-06-0646)~~
文摘[Objective] The aim was to isolate and identify a taxol-producing endophytic fungus from Taxus media. [Method] 32 strains of endophytic fungi were identified form the inner bark of T. media,and their fermentation products were detected by high performance liquid chromatography (HPLC). [Result] Through the screening,a strain of taxol-producing endophytic fungi M57 was obtained,which could produce 45-50 μg/L of taxol,and M57 was defined as Rhizopus sp. through morphological observation and 18S rDNA sequence analysis. [Conclusion] The finding of Rhizopus sp. M57 provided a promising strain for producing taxol with taxol-producing fungi fermentation process.
基金Supported by Natural Science Foundation of Chongqing City(CSTC,2009BB1294)~~
文摘[ Objective ] The paper was to study the growth-promoting and antagonistic action of endophytic bacteria strains Itb57 and Itb295 of tobacco to explore their functions in biological control. [ Method] The growth-promoting effects of bacterial suspension ~ff endophytic bacteria Itb57 and Itb295 on tobacco seedling un- der different treatment modes were studied using potting method in greenhouse. The antagonistic action of bacterial suspension of endophytic bacteria Itb57 and Itb295 on Phytophthora nicotianae, Alternaria alternata and Botrytis cinerea were measured by duel culture method. [ Result] Bacterial suspensions of enduphytic bacteria Itb57 and Itb295 had certain growth-promoting effects on tobacco seedling, which could significantly increase the fresh weight and dry weight in aerial part; the growth-promoting effect of soaking ＋ spraying and irrigating treatment was the best. Itb57 strain had good antagonistic action against P. nicotianae. A. alterna- ta and B. cinerea, while Itb295 strain only had good antifungal effect against P. nicotianae. [ Conclusion] The results provided basis for the study and application of tobacco endophytic bacteria strains Itb57 and Itb295 in biocontrol of tobacco diseases.
基金Supported by National Natural Science Foundation of China(Grant No.30960178)Natural Science Foundation of Xinjiang Province(Grant No.200821126)~~
文摘[Objective] This study aimed to investigate the feasibility of agricultural irri- gation with reclaimed water in Urumqi City. [Method] Chinese cabbages were used as experimental materials and irrigated with control water, 50% reclaimed water and 100% reclaimed water, and then the number of endophytic bacteria in Chinese cab- bages was measured. [Result] Using 50% reclaimed water, the fresh weight of Chi- nese cabbage was improved by 68.94%; however, with the deepening internalization of exogenous microorganism internalization, the total number of endophytic bacteria and coliform flock in Chinese cabbages treated with 50% reclaimed water was sig- nificantly higher than the other two treatment groups （P〈0.05）. [Conclusion] Re- claimed water enhances both the weight of Chinese cabbage and the number of endophytic bacteria, which is unsuitable for irrigation of edible crops such as Chi- nese cabbage. Strengthening disinfection during the process of sewerage treatment could solve this problem.
基金Supported by National Natural Science Foundation of China(No.31000144)Project of Shaanxi Provincial Science and Technology Department(No.2012JQ3017)+1 种基金Project of Xi'an Science and Technology Bureau[NC1206(3)]Project of Education Department of Shaanxi Provincial Government(No.12JK1106)~~
文摘[Objective] This study aimed to isolate and screen black-spot-resistant en- dophytic fungi strains. [Method] Various species of healthy Rosa chinensis were col- lected from Xi＇an, Xianyang, Baoji and Weinan City in Shaanxi Province. Endophytic fungi was isolated from stems and leaves, and purified by 2-3 times of inoculation to screen endophytic fungi antagonistic to Marssonina rosae with modified punching method. [Result] Samples collected from Xianyang exhibited the highest colonization rate and isolation rate; endophytic fungi strains isolated from stems presented the highest colonization rate and isolation rate compared with leaves. A total of 67 en- dophytic fungi strains were isolated from Rosa chinensis, including 3 black spot-re- sistant strains which were all derived from Baoji. [Conclusion] This study laid the foundation for further screening candidate strains of biocontrol fungi and environ- ment-friendly fungal biological control.
基金Supported by National Natural Science Foundation of China(30960010)KeyProject of Principal Fund in Tarim University(TDZKZD06001)~~
文摘[ Objective ] The paper was to study and analyze the genetic diversity of 48 endophytic bacteria isolated from Sophora alopecuroide with strong antagonis- tic effect against Fusarium oxysporum and Verticillium dahliae, and carry out sequence measurement and phylogenetic analysis on 10 representative strains. [ Meth- od] Using total DNA ERIC-PCR and 16S rRNA sequencing analysis method, a total of 48 endophytic bacteria were carried out genetic diversity and phylogenetic analysis. [Result] ERIC-PCR fingerprint map showed that 48 endophytic bacteria were divided into 6 ERIC groups and 2 strains with independent groups at Wat- son distance of 0.31. Representative strains were selected from each group to determine 16S rRNA gene for phylogenetic analysis, and the results showed that these strains belonged to Bacillus atrophaeus, Bacillus subtilis, Bacillus cereus, Bacillus megaterium, Bacillus pumilus and Serratia marcescens, respectively. [ Conclu- sion ] The genetic diversity of 48 strains of bacteria was obvious. Determination of 48 strains of bacteria on diversity and phylogenetic status could lay foundation for study on mechanism of F. oxysporum and V. dahliae, which could also provide new strain sources for biological control of diseases in cotton production.
基金Supported by National Natural Science Foundation of China(31060193)Starting Foundation of Scientific research in Guilin University of Technology~~
文摘[Objective] The aim was to separate and purify antioxidant endophytic fun- gi from Stevia leaves. [Method] Stevia leaves were used as materials for endophytic fungi screening and purification. Then, liquid culture was conducted to test fermented supernatant abilities of antioxidant, DPPH free radical clearing and chemilumines- cence inhibition. [Result] Forty two endophytic fungi were separated from F01 to F42. F41 was proved to be the strongest in antioxidant aspect; F37 was the strongest in DPPH clearing; F02, F07, F20, F25, F37, and F416 stood out in a comprehensive comparison of the above aspects. Flow injection chemiluminescenct method was used to test the above six fermentation liquors, and the results showed that antioxidant abilities of the six were quite strong for all of chemiluminescence rates were over 70%. Through preliminary morphological identification, bacterial colony was found white or yellowy and only F02 was of dissepiment. [Conclusion] Fungi with high-antioxidation activities were separated, which paved the way for future research.
基金Supported by Platform Project of the Ministry of Science and Technology of China for Microorganism Resources(2005DKA2120721)~~
文摘[Objective] The study was aimed to provide theoretical foundation for developing a new fungicide with high activity,low toxicity and low residua.[Method] Based on the plant growth rate and inhibition zone methods,the antifungal activity of extracts of every organ of Xanthium sibiricum,the fermentation liquid of endophytic fungi MC4-23,and the extract of mycelium were tested with six kinds of pathogenic fungi and three kinds of pathogenic bacteria respectively.[Result] The extract of every organ of Xanthium sibiricum,the fermentation liquid of endophyic fungi MC4-23,and the extract of mycelium all had antifungal activities.The acetone extracts of Xanthium sibiricum had stronger antifungal activities than the ethanol extracts of Xanthium sibiricum.The extracts of leaf have the strongest antifungal activities against fungi and bacteria.The inhibition rates of the six pathogenic fungi were all above 47.8%,and the inhibition diameters of the three pathogenic bacteria were all above 0.5 centimeter.The fermentation liquid of endophytic fungi also had strong activities against the fungi and bacteria.The inhibition rates of the six pathogenic fungi were all above 68.1%.The extracts of root of Xanthium sibiricum and the mycelium of endophytic fungi were all had antifungal activities.[Conclusion] Xanthium sibiricum and endophyic fungi MC4-23 had great value of development and utilization.
文摘A total of 94 isolates of endophytic fungi were isolated from the bark of 200-yr.-old Taxus cuspidata Sieb. et Zucc. in the primeval forest of the Changbai Mountain Natural Reserve, and 19 species of endophytic fungi were identified. Among the taxa 2 new species and 1 new varied species as well as the 6 known species of China had been described in a previous article published on Journal of Forestry Research, Vol. 14 (4): 290-294 (2003). This paper made a detail description on the morpho-logical characteristics of the 9 new recorded-genus-species and 1 new record species with illustrations, including Staphy-lotrichum coccosporum Meyer et Nicot, Botryodema lateritium Papendoorf & Upadhyay, Oedocephalum glomerulosum (Rul-liard) Sacc., Trichosporonoides oedocephalis Haskins & Spencer, Beniowskia sphaeroidea (Kal Chbrenner et Caeke) Mason, Thermomyces verrucosus Pugh, Blakeman & Morgan-jones, Gilmaniella humicola Brron , Arthrinium sphaerosperma (Per.:Fr.) Ellis, Ambrosiella brunnea (Verrall) Batra, and Cytosporina notha Diedz (1 new record species). And the habitats, hosts, distribu-tions and fruiting periods were recorded for the new recorded-genus-species. The determination result of thin layer chroma-tography (TLC) showed that the liquid fermentation metabolites of the strains could not react positively with taxoids developer - the vanillic aldehyde and dilute bismuth potassium iodide.
文摘[Objective] This study aimed to isolate the endophytic fungi of Paris polyphylla var. yunnanensis and investigate their effects on the embryo development of P. polyphylla var. yunnanensis seeds. [Method] The endophytic fungi of P. polyphylla were isolated and identified morphologically, and their effects on the embryo development of P. polyphylla var. yunnanensis seeds were studied by using paraffin sectioning and microphotography. [Result] Nine endophytic fungi, i.e. P. polyphylla var. yunnanensis endophytic fungi PPYEF-1, PPYEF-2, PPYEF-3, PPYEF-4, PPYEF-5, PPYEF-6, PPYEF-7, PPYEF-8 and PPYEF-9 belonging to seven genera in five families, three orders were isolated from the rhizomes. Except PPYEF-4 (Cladosporium sp.), other fungi could promote the embryo development of the P. polyphylla var. yunnanensis seeds, mostly reaching the extremely significant or significant level. PPYEF-9 (Trichoderma sp.) resulted in the highest embryo length and embryo-emerging ratio. [Conclusion] This paper could provide a reference for the application of the endophytic fungi of P. polyphylla var. yunnanensis in the dormancy-breaking of P. polyphylla var. yunnanensis seeds.
基金Supported by the National Natural Science Foundation of China(31070443,30970523)the National Science Foundation for Talents Training in Basic Science,China(J1103507)the Project of the Priority Academic Program Development of Jiangsu Higher Education Institutions,China~~
文摘[Objective] To examine the effect of endophytic fungi ALl2 （Gilmaniella sp.） on metabolites distribution in organs of Atractylodes lancea. [Method] Endophytic fungi ALl2 was inoculated on Atraetylodes lancea plantlets in tissue culture, and the distribution of cellulose, hemicellulose, lignin, soluble sugar in leaves and roots of the inoculated group were detected. The weight of leaves and roots were compared. Gas Chromatography was used to analyze the volatile oil components. [Result] Compared with the control group, the average fresh weight and dry weight of leaves and roots of A. lancea which had been symbiosed with ALl2 increased significantly. The content of lignin and soluble sugar increased in the leaves of the inoculated group, and the content of cellulose, hemicellulose, lignin, soluble sugar and volatile oil also increased in roots. [Conclusion] The results indicate that symbiosed with ALl2 is benefit for the development of A. lancea roots and can promote the transfer and accumulation of the medicinal components to the roots.
