Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied in the detection of the end point temperature (EPT) of thermal denatured protein in fish and meat in this study. It was also used in stu...Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied in the detection of the end point temperature (EPT) of thermal denatured protein in fish and meat in this study. It was also used in studying the thermal denatured temperature range of proteins in salmon and chicken meat. The results show that the temperature ranges of denatured proteins were from 65 ℃ to 75 ℃ , and these temperature ranges were influenced by the processing methods. Through SDS-PAGE, the features of repeated heating thermal denatured proteins under the same temperature and processing time were studied. The electrophoresis patterns of thermal denatured proteins determined through repeated heating at the same temperature did not exhibit any change. For the detection of cooked fish and meat samples, they were subjected to applying the SDS-PAGE method, which revealed an EPT ranging from 60 ℃ to 80 ℃ .展开更多
We calculate the quark number susceptibility （QNS） around the chiral critical end point （CEP）. The CEP is found to be located at （μc,Tc）= （80 MeV, 148 MeV） where μc and Tc are the critical chemical potential...We calculate the quark number susceptibility （QNS） around the chiral critical end point （CEP）. The CEP is found to be located at （μc,Tc）= （80 MeV, 148 MeV） where μc and Tc are the critical chemical potential and temperature, respectively. The QNS is found to have the highest and sharpest peak at the CEP. It is also found that, when the chemical potential μ is in the range of 60MeV≤ μ ≤ 110MeV, the QNS near the transition temperature is larger than the free field result, which indicates that the space-like damping mode dominates the degree of freedom of motion near the CEP.展开更多
AIM： To review the defnition and performance of the commonly used end points in trials of systemic thera-pies in metastatic castration-resistant prostate cancer patients. METHODS： A literature search was undertaken...AIM： To review the defnition and performance of the commonly used end points in trials of systemic thera-pies in metastatic castration-resistant prostate cancer patients. METHODS： A literature search was undertaken on PubMed database to identify studies meeting estab-lished criteria, with the aim of selecting randomized clinical trials and study definition and performance of their end points. The end points were grouped into three categories： overall survival （OS）, time-to-event end points, and response end points. A special analysis was performed for secondary end points of the studies which documented a beneft in OS in the experimental arm. Finally, publishes analyses for surrogacy of the in-cluded end points were also reported. RESULTS： OS, time-to-event and response end points in 31 selected trials were analyzed. OS was the pri-mary end point in 14 trials, and the secondary end point in 17. A time-to-event end point was the primaryend point in 8 studies, and the secondary end point in 22; the most reported time-to-event end points were composite end points, and the events changed among trials. A response end point was the primary end point in 9 studies, in 3 it was prostate-specifc antigen （PSA）-related, in 3 pain-related and in 3 mixed. A response end point was the secondary end point in 19 studies： PSA response and radiologic response were the most frequently used secondary end points in 19 and 11 tri-als, respectively, while pain response was used in 5 studies.CONCLUSION： A homogeneous defnition of progres-sion in future trials is mandatory. Among response end points, pain-response and PSA-response appear to be the most reliable.展开更多
We investigate the baryon number susceptibilities up to fourth order along different freeze-out lines in a holographic QCD model with a critical end point（CEP）, and we propose that the peaked baryon number susceptib...We investigate the baryon number susceptibilities up to fourth order along different freeze-out lines in a holographic QCD model with a critical end point（CEP）, and we propose that the peaked baryon number susceptibilities along the freeze-out line can be used as a clean signature to locate the CEP in the QCD phase diagram.On the temperature and baryon chemical potential plane, the cumulant ratio of the baryon number susceptibilities（up to fourth order） forms a ridge along the phase boundary, and develops a sword-shaped ＂mountain＂ standing upright around the CEP in a narrow and oblate region. The measurement of baryon number susceptibilities from heavy-ion collision experiments is along the freeze-out line. If the freeze-out line crosses the foot of the CEP mountain, then one can observe the peaked baryon number susceptibilities along the freeze-out line, and the kurtosis of the baryon number distributions has the highest magnitude. The data from the first phase of the beam energy scan program at the Relativistic Heavy Ion Collider indicates that there should be a peak of the kurtosis of the baryon number distribution at a collision energy of around 5 Ge V, which suggests that the freeze-out line crosses the foot of the CEP mountain and the summit of the CEP should be located nearby, around a collision energy of 3–7 GeV.展开更多
We investigated different entanglement properties of a holographic QCD(hQCD)model with a critical end point at the finite baryon density.Firstly,we considered the holographic entanglement entropy(HEE)of this hQCD mode...We investigated different entanglement properties of a holographic QCD(hQCD)model with a critical end point at the finite baryon density.Firstly,we considered the holographic entanglement entropy(HEE)of this hQCD model in a spherical shaped region and a strip shaped region.It was determined that the HEE of this hQCD model in both regions can reflect QCD phase transition.Moreover,although the area formulas and minimal area equations of the two regions were quite different,the HEE exhibited a similar behavior on the QCD phase diagram.Therefore,we assert that the behavior of the HEE on the QCD phase diagram is independent of the shape of the subregions.However,the HEE is not an ideal parameter for the characterization of the entanglement between different subregions of a thermal system.As such,we investigated the mutual information(MI),conditional mutual information(CMI),and the entanglement of purification(Ep)in different strip shaped regions.We determined that the three entanglement quantities exhibited some universal behavior;their values did not change significantly in the hadronic matter phase but increased rapidly with the increase in T andμin the QGP phase.Near the phase boundary,these three entanglement quantities changed smoothly in the crossover region and continuously but not smoothly at CEP;they exhibited discontinuous behavior in the first phase transition region.These properties can be used to distinguish between the different phases of strongly coupled matter.展开更多
This paper considered the optimal control problem for distributed parameter systems with mixed phase-control constraints and end-point constraints. Pontryagin's maximum principle for optimal control are derived vi...This paper considered the optimal control problem for distributed parameter systems with mixed phase-control constraints and end-point constraints. Pontryagin's maximum principle for optimal control are derived via Duboviskij-Milujin theorem.展开更多
A method of robust speech endpoint detection in airplane cockpit voice background is presented. Based on the analysis of background noise character, a complex Laplacian distribution model directly aiming at noisy spee...A method of robust speech endpoint detection in airplane cockpit voice background is presented. Based on the analysis of background noise character, a complex Laplacian distribution model directly aiming at noisy speech is established. Then the likelihood ratio test based on binary hypothesis test is carried out. The decision criterion of conventional maximum a posterior incorporating the inter-frame correlation leads to two separate thresholds. Speech endpoint detection decision is finally made depend on the previous frame and the observed spectrum, and the speech endpoint is searched based on the decision. Compared with the typical algorithms, the proposed method operates robust in the airplane cockpit voice background.展开更多
The present study aims to analyze the shift in shoreline due to coastal processes and formulate available for best estimate of future shoreline positions based on precedent shorelines. Information on rates and trends ...The present study aims to analyze the shift in shoreline due to coastal processes and formulate available for best estimate of future shoreline positions based on precedent shorelines. Information on rates and trends of shoreline change can be used to improve the understanding of the underlying causes and potential effects of coastal erosion which can support informed coastal management decisions. In this paper, researchers go over the changes in the recent positions of the shoreline of the Balasore coast for the 38 years from 1975 through 2013. The study area includes the Balasore coastal region from Rasalpur to Udaypur together with Chandipur, Choumukh, Chandrabali as well as Bichitrapur. Transects wise shoreline data base were developed for approximately 67 kilometers of shoreline and erosional/accretional scenario has also been analysed by delineating the shoreline from Landsat imageries of 1975, 1980, 1990, 1995, 2000, 2005, 2010 and 2013. A simple Linear Regression Model and End Point Rate (EPR) have been adopted to take out the rate of change of shoreline and its future positions, based on empirical observations at 67 transects along the Balasore coast. It is found that the north eastern part of Balasore coast in the vicinity of Subarnarekha estuary and Chandrabali beach undergo high rates of shore line shift. The shoreline data were integrated for long- (about 17 years) and short-term (about 7 years) shift rates analysis to comprehend the shoreline change and prediction. For the prediction of future shoreline, the model has been validated with the present shoreline position (2013). The rate of shoreline movement calculated from the fixed base line to shoreline position of 1975, 1980, 1990, 1995, 2000, 2005 and 2010 and based on this, the estimated shoreline of 2013 was calculated. The estimated shoreline was compared with the actual shoreline delineated from satellite imagery of 2013. The model error or positional shift at each sample point is observed. The positional error varies from??4.82 m to 212.41 m. It has been found that model prediction error is higher in the left hand side of river Subarnarekha. The overall error for the entire predicted shoreline was found to be 41.88 m by Root Mean Square Error (RMSE). In addition, it was tested by means difference between actual and predicted shoreline positions using “t” test and it has been found that predicted shore line is not significantly different from actual shoreline position at (t132 = 0.278) p < 0.01.展开更多
Discrete and symmetric three-dimensional（3D） DNA nanocages have been revoked as excellent candidates for various applications,such as vip component encapsulation and organization（e.g.dye molecules,proteins,inorga...Discrete and symmetric three-dimensional（3D） DNA nanocages have been revoked as excellent candidates for various applications,such as vip component encapsulation and organization（e.g.dye molecules,proteins,inorganic nanoparticles,etc.） to construct new materials and devices.To date,a large variety of DNA nanocages has been synthesized through assembling small individual DNA motifs into predesigned structures in a bottom-up fashion.Most of them rely on the assembly using multiple copies of single type of motifs and a few sophisticated nanostructures have been engineered by co-assembling multi-types of DNA tiles simultaneously.However,the availability of complex DNA nanocages is still limited.Herein,we demonstrate that highly symmetric DNA nanocages consisted of binary DNA pointstar motifs can be easily assembled by deliberately engineering the sticky-end interaction between the component building blocks.As such,DNA nanocages with new geometries,including elongated tetrahedron（E-TET）,rhombic dodecahedron（R-DOD）,and rhombic triacontahedron（R-TRI） are successfully synthesized.Moreover,their design principle,assembly process,and structural features are revealed by polyacryalmide gel electrophoresis（PAGE）,atomic force microscope（AFM） imaging,and cryogenic transmission electron microscope imaging（cryo-TEM） associated with single particle reconstruction.展开更多
基金supported by a research project (No. 2006IK012) of the General Administration of Quality Supervision, Inspection and Quarantine of P. R. China.
文摘Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied in the detection of the end point temperature (EPT) of thermal denatured protein in fish and meat in this study. It was also used in studying the thermal denatured temperature range of proteins in salmon and chicken meat. The results show that the temperature ranges of denatured proteins were from 65 ℃ to 75 ℃ , and these temperature ranges were influenced by the processing methods. Through SDS-PAGE, the features of repeated heating thermal denatured proteins under the same temperature and processing time were studied. The electrophoresis patterns of thermal denatured proteins determined through repeated heating at the same temperature did not exhibit any change. For the detection of cooked fish and meat samples, they were subjected to applying the SDS-PAGE method, which revealed an EPT ranging from 60 ℃ to 80 ℃ .
基金Supported by the National Natural Science Foundation of China under Grant Nos 11105122,11275097 and 11475085the Foundation of Graduate School of Nanjing University under Grant No 2014CL02
文摘We calculate the quark number susceptibility （QNS） around the chiral critical end point （CEP）. The CEP is found to be located at （μc,Tc）= （80 MeV, 148 MeV） where μc and Tc are the critical chemical potential and temperature, respectively. The QNS is found to have the highest and sharpest peak at the CEP. It is also found that, when the chemical potential μ is in the range of 60MeV≤ μ ≤ 110MeV, the QNS near the transition temperature is larger than the free field result, which indicates that the space-like damping mode dominates the degree of freedom of motion near the CEP.
文摘AIM： To review the defnition and performance of the commonly used end points in trials of systemic thera-pies in metastatic castration-resistant prostate cancer patients. METHODS： A literature search was undertaken on PubMed database to identify studies meeting estab-lished criteria, with the aim of selecting randomized clinical trials and study definition and performance of their end points. The end points were grouped into three categories： overall survival （OS）, time-to-event end points, and response end points. A special analysis was performed for secondary end points of the studies which documented a beneft in OS in the experimental arm. Finally, publishes analyses for surrogacy of the in-cluded end points were also reported. RESULTS： OS, time-to-event and response end points in 31 selected trials were analyzed. OS was the pri-mary end point in 14 trials, and the secondary end point in 17. A time-to-event end point was the primaryend point in 8 studies, and the secondary end point in 22; the most reported time-to-event end points were composite end points, and the events changed among trials. A response end point was the primary end point in 9 studies, in 3 it was prostate-specifc antigen （PSA）-related, in 3 pain-related and in 3 mixed. A response end point was the secondary end point in 19 studies： PSA response and radiologic response were the most frequently used secondary end points in 19 and 11 tri-als, respectively, while pain response was used in 5 studies.CONCLUSION： A homogeneous defnition of progres-sion in future trials is mandatory. Among response end points, pain-response and PSA-response appear to be the most reliable.
基金Supported by NSFC(11275213,and 11261130311)(CRC 110 by DFG and NSFC)CAS key project KJCX2-EW-N01Youth Innovation Promotion Association of CAS
文摘We investigate the baryon number susceptibilities up to fourth order along different freeze-out lines in a holographic QCD model with a critical end point（CEP）, and we propose that the peaked baryon number susceptibilities along the freeze-out line can be used as a clean signature to locate the CEP in the QCD phase diagram.On the temperature and baryon chemical potential plane, the cumulant ratio of the baryon number susceptibilities（up to fourth order） forms a ridge along the phase boundary, and develops a sword-shaped ＂mountain＂ standing upright around the CEP in a narrow and oblate region. The measurement of baryon number susceptibilities from heavy-ion collision experiments is along the freeze-out line. If the freeze-out line crosses the foot of the CEP mountain, then one can observe the peaked baryon number susceptibilities along the freeze-out line, and the kurtosis of the baryon number distributions has the highest magnitude. The data from the first phase of the beam energy scan program at the Relativistic Heavy Ion Collider indicates that there should be a peak of the kurtosis of the baryon number distribution at a collision energy of around 5 Ge V, which suggests that the freeze-out line crosses the foot of the CEP mountain and the summit of the CEP should be located nearby, around a collision energy of 3–7 GeV.
基金support by the NSFC(11947233)CPSF(2019M662507)+4 种基金the start-up funding from Zhengzhou Universitysupported in part by the NSFC(11725523,11735007,11261130311)(CRC 110 by DFG and NSFC)Chinese Academy of Sciences(XDPB09)the Fundamental Research Funds for the Central Universitiesthe start-up funding from University of Chinese Academy of Sciences。
文摘We investigated different entanglement properties of a holographic QCD(hQCD)model with a critical end point at the finite baryon density.Firstly,we considered the holographic entanglement entropy(HEE)of this hQCD model in a spherical shaped region and a strip shaped region.It was determined that the HEE of this hQCD model in both regions can reflect QCD phase transition.Moreover,although the area formulas and minimal area equations of the two regions were quite different,the HEE exhibited a similar behavior on the QCD phase diagram.Therefore,we assert that the behavior of the HEE on the QCD phase diagram is independent of the shape of the subregions.However,the HEE is not an ideal parameter for the characterization of the entanglement between different subregions of a thermal system.As such,we investigated the mutual information(MI),conditional mutual information(CMI),and the entanglement of purification(Ep)in different strip shaped regions.We determined that the three entanglement quantities exhibited some universal behavior;their values did not change significantly in the hadronic matter phase but increased rapidly with the increase in T andμin the QGP phase.Near the phase boundary,these three entanglement quantities changed smoothly in the crossover region and continuously but not smoothly at CEP;they exhibited discontinuous behavior in the first phase transition region.These properties can be used to distinguish between the different phases of strongly coupled matter.
文摘This paper considered the optimal control problem for distributed parameter systems with mixed phase-control constraints and end-point constraints. Pontryagin's maximum principle for optimal control are derived via Duboviskij-Milujin theorem.
文摘A method of robust speech endpoint detection in airplane cockpit voice background is presented. Based on the analysis of background noise character, a complex Laplacian distribution model directly aiming at noisy speech is established. Then the likelihood ratio test based on binary hypothesis test is carried out. The decision criterion of conventional maximum a posterior incorporating the inter-frame correlation leads to two separate thresholds. Speech endpoint detection decision is finally made depend on the previous frame and the observed spectrum, and the speech endpoint is searched based on the decision. Compared with the typical algorithms, the proposed method operates robust in the airplane cockpit voice background.
文摘The present study aims to analyze the shift in shoreline due to coastal processes and formulate available for best estimate of future shoreline positions based on precedent shorelines. Information on rates and trends of shoreline change can be used to improve the understanding of the underlying causes and potential effects of coastal erosion which can support informed coastal management decisions. In this paper, researchers go over the changes in the recent positions of the shoreline of the Balasore coast for the 38 years from 1975 through 2013. The study area includes the Balasore coastal region from Rasalpur to Udaypur together with Chandipur, Choumukh, Chandrabali as well as Bichitrapur. Transects wise shoreline data base were developed for approximately 67 kilometers of shoreline and erosional/accretional scenario has also been analysed by delineating the shoreline from Landsat imageries of 1975, 1980, 1990, 1995, 2000, 2005, 2010 and 2013. A simple Linear Regression Model and End Point Rate (EPR) have been adopted to take out the rate of change of shoreline and its future positions, based on empirical observations at 67 transects along the Balasore coast. It is found that the north eastern part of Balasore coast in the vicinity of Subarnarekha estuary and Chandrabali beach undergo high rates of shore line shift. The shoreline data were integrated for long- (about 17 years) and short-term (about 7 years) shift rates analysis to comprehend the shoreline change and prediction. For the prediction of future shoreline, the model has been validated with the present shoreline position (2013). The rate of shoreline movement calculated from the fixed base line to shoreline position of 1975, 1980, 1990, 1995, 2000, 2005 and 2010 and based on this, the estimated shoreline of 2013 was calculated. The estimated shoreline was compared with the actual shoreline delineated from satellite imagery of 2013. The model error or positional shift at each sample point is observed. The positional error varies from??4.82 m to 212.41 m. It has been found that model prediction error is higher in the left hand side of river Subarnarekha. The overall error for the entire predicted shoreline was found to be 41.88 m by Root Mean Square Error (RMSE). In addition, it was tested by means difference between actual and predicted shoreline positions using “t” test and it has been found that predicted shore line is not significantly different from actual shoreline position at (t132 = 0.278) p < 0.01.
基金financially supported by the National Natural Science Foundation of China(Nos.21504053,21673139,and91527304)the Program of Shanghai Medical Professionals Across Subject Funds(No.YG2016MS74)the Recruitment Program of Global Experts(No.15Z127060012)
文摘Discrete and symmetric three-dimensional（3D） DNA nanocages have been revoked as excellent candidates for various applications,such as vip component encapsulation and organization（e.g.dye molecules,proteins,inorganic nanoparticles,etc.） to construct new materials and devices.To date,a large variety of DNA nanocages has been synthesized through assembling small individual DNA motifs into predesigned structures in a bottom-up fashion.Most of them rely on the assembly using multiple copies of single type of motifs and a few sophisticated nanostructures have been engineered by co-assembling multi-types of DNA tiles simultaneously.However,the availability of complex DNA nanocages is still limited.Herein,we demonstrate that highly symmetric DNA nanocages consisted of binary DNA pointstar motifs can be easily assembled by deliberately engineering the sticky-end interaction between the component building blocks.As such,DNA nanocages with new geometries,including elongated tetrahedron（E-TET）,rhombic dodecahedron（R-DOD）,and rhombic triacontahedron（R-TRI） are successfully synthesized.Moreover,their design principle,assembly process,and structural features are revealed by polyacryalmide gel electrophoresis（PAGE）,atomic force microscope（AFM） imaging,and cryogenic transmission electron microscope imaging（cryo-TEM） associated with single particle reconstruction.
