The complete nucleotide sequence of an isolate of Citrus vein enation virus（CVEV-XZG） from China has been determined for the first time. The genome consisted of 5 983 nucleotides, coding for five open reading frames...The complete nucleotide sequence of an isolate of Citrus vein enation virus（CVEV-XZG） from China has been determined for the first time. The genome consisted of 5 983 nucleotides, coding for five open reading frames（ORFs）, had a similar genomic organization features with Pea enation mosaic virus（PEMV）. Nucleotide and deduced amino acid sequence identity of the five ORFs compared to isolate CVEV VE-1 range from 97.1 to 99.0% and 97.4 to 100.0%, these values compared to isolate PEMV-1 range from 45.2 to 51.6% and 31.1 to 45.2%. Phylogenetic analysis based on the complete genome sequence showed that the isolate CVEV-XZG had close relationship with Pea enation mosaic virus. The results supports CVEV may be a new member of genus Enamovirus. The full sequence of CVEV-XZG presented here may serve as a basis for future study of CVEV in China.展开更多
The pea enation mosaic disease(PEMD)causes significant yield losses worldwide.PEMD is caused by two taxonomically unrelated but symbiotic viruses,pea enation mosaic virus 1(PEMV-1)and pea enation mosaic virus 2(PEMV-2...The pea enation mosaic disease(PEMD)causes significant yield losses worldwide.PEMD is caused by two taxonomically unrelated but symbiotic viruses,pea enation mosaic virus 1(PEMV-1)and pea enation mosaic virus 2(PEMV-2).In this study,the complete genomes of four isolates of PEMV-1 and PEMV-2 from Yunnan Province of China were determined and analyzed.The four isolates of PEMV-1 or PEMV-2 shared≥98.7%nucleotide sequence identities at whole genome level.The four Chinese PEMV-1 isolates were most closely related to PEMV-1-Ramsey-1 with nucleotide sequence identities of 97.8–98.0%,while the four Chinese PEMV-2 isolates were most closely related to PEMV-2-DSMZ PV-0088,with nucleotide sequence identities of 94.3–94.5%.The two full-length infectious cDNA clones of the Yunnan-Dali isolates of the two viruses(PEMV-1-YDL and PEMV-2-YDL)were constructed.Co-inoculation of PEMV-1-YDL and PEMV-2-YDL in pea seedlings caused systemic infection,with typical enation and mosaic symptoms on new leaves after inoculation,while the inoculated Nicotiana benthamiana plants showed curling symptoms.Inoculation of pea seedlings with either PEMV-1-YDL or PEMV-2-YDL did not cause obvious symptoms on the new leaves.It has been known that PEMV-2 helps the systemic infection of PEMV-1.However,our results showed that PEMV-1-YDL alone could cause a systemic infection.The pathogenicity of the two infectious clones was tested on different pea cultivars,and some cultivars were PEMD-tolerant.This is the first report of the infectious clones constructed from the Chinese isolates of PEMV-1 and PEMV-2.The current approach will facilitate determination of the synergistic relationship between PEMV-1 and PEMV-2 and their interactions with the host plant by reverse genetic analysis.展开更多
基金funded by the Chongqing Natural Science Foundation Project, China (cstc2011jj A80025)
文摘The complete nucleotide sequence of an isolate of Citrus vein enation virus（CVEV-XZG） from China has been determined for the first time. The genome consisted of 5 983 nucleotides, coding for five open reading frames（ORFs）, had a similar genomic organization features with Pea enation mosaic virus（PEMV）. Nucleotide and deduced amino acid sequence identity of the five ORFs compared to isolate CVEV VE-1 range from 97.1 to 99.0% and 97.4 to 100.0%, these values compared to isolate PEMV-1 range from 45.2 to 51.6% and 31.1 to 45.2%. Phylogenetic analysis based on the complete genome sequence showed that the isolate CVEV-XZG had close relationship with Pea enation mosaic virus. The results supports CVEV may be a new member of genus Enamovirus. The full sequence of CVEV-XZG presented here may serve as a basis for future study of CVEV in China.
基金supported by the Science and Technology Project of Yunnan Province,China(202005AF150040).
文摘The pea enation mosaic disease(PEMD)causes significant yield losses worldwide.PEMD is caused by two taxonomically unrelated but symbiotic viruses,pea enation mosaic virus 1(PEMV-1)and pea enation mosaic virus 2(PEMV-2).In this study,the complete genomes of four isolates of PEMV-1 and PEMV-2 from Yunnan Province of China were determined and analyzed.The four isolates of PEMV-1 or PEMV-2 shared≥98.7%nucleotide sequence identities at whole genome level.The four Chinese PEMV-1 isolates were most closely related to PEMV-1-Ramsey-1 with nucleotide sequence identities of 97.8–98.0%,while the four Chinese PEMV-2 isolates were most closely related to PEMV-2-DSMZ PV-0088,with nucleotide sequence identities of 94.3–94.5%.The two full-length infectious cDNA clones of the Yunnan-Dali isolates of the two viruses(PEMV-1-YDL and PEMV-2-YDL)were constructed.Co-inoculation of PEMV-1-YDL and PEMV-2-YDL in pea seedlings caused systemic infection,with typical enation and mosaic symptoms on new leaves after inoculation,while the inoculated Nicotiana benthamiana plants showed curling symptoms.Inoculation of pea seedlings with either PEMV-1-YDL or PEMV-2-YDL did not cause obvious symptoms on the new leaves.It has been known that PEMV-2 helps the systemic infection of PEMV-1.However,our results showed that PEMV-1-YDL alone could cause a systemic infection.The pathogenicity of the two infectious clones was tested on different pea cultivars,and some cultivars were PEMD-tolerant.This is the first report of the infectious clones constructed from the Chinese isolates of PEMV-1 and PEMV-2.The current approach will facilitate determination of the synergistic relationship between PEMV-1 and PEMV-2 and their interactions with the host plant by reverse genetic analysis.
