EMulate Therapeutics has developed a system for emulating the effects of solvated molecules via their magnetic field recordings. Recordings of magnetic field emissions of select small inhibitor RNAs (siRNAs;murine tar...EMulate Therapeutics has developed a system for emulating the effects of solvated molecules via their magnetic field recordings. Recordings of magnetic field emissions of select small inhibitor RNAs (siRNAs;murine targeting CTLA-4 and murine targeting PD-1) were tested on C57Bl/6 mice implanted subcutaneously with the GL261 murine tumor cell line. A signal composed of concatenated recordings of siRNA molecules targeting the murine CTLA-4 and PD-1 receptors (labeled A2) was used in immune competent C57Bl/6 mice. The mice were flank implanted with the murine glioblastoma cell line GL261. Mice were exposed to the signal continuously (24 hours a day) until tumor volumes reached the designated volume limit. Tumors were excised and analyzed via PAGE/Western blot for the expression of CTLA-4, PD-1, Ki67, Caspase 3, CD4 and CD8. Terminal blood draws were used for CBCs. We report the down regulation of the checkpoint inhibitors CTLA-4 in the exposed mice. Significant tumor volume reduction was observed in mice exposed to the siRNA signal compared to control mice;no adverse events were recorded. Cell blood counts (CBC) and protein expression patterns were observed to correlate with the expected function of protein expression inhibition of the targets.展开更多
Blockchain,like any other complex technology,needs a strong testing methodology to support its evolution in both research and development contexts.Setting up meaningful tests for permissionless blockchain technology i...Blockchain,like any other complex technology,needs a strong testing methodology to support its evolution in both research and development contexts.Setting up meaningful tests for permissionless blockchain technology is a notoriously complex task for several reasons:software is complex,a large number of nodes are involved,the network is non-ideal,etc.Developers usually adopt small virtual laboratories or costly real devnets based on real software.Researchers usually prefer simulations of a large number of nodes based on simplified models.In this paper,we aim to obtain the advantages of both approaches,i.e.,performing large,realistic,inexpensive,and flexible experiments,using real blockchain software within a virtual environment.To do that,we address the challenge of running large blockchain networks in a single physical machine,leveraging Linux and Docker.We analyze a number of problems that arise when large blockchain networks are emulated,and we provide technical solutions for all of them.Finally,we describe two experiences of emulating fairly large blockchain networks on a single machine:adopting both research-oriented and production-oriented software and involving more than 3000 containers.展开更多
This paper presents a space network emulation system based on a user-space network stack named Nos to solve space networks'unique architecture and routing issues and kernel stacks'inefficiency and development ...This paper presents a space network emulation system based on a user-space network stack named Nos to solve space networks'unique architecture and routing issues and kernel stacks'inefficiency and development complexity.Our low Earth orbit satellite scenario emulation verifies the dynamic routing function of the protocol stack.The proposed system uses technologies like Open vSwitch(OVS)and traffic control(TC)to emulate the space network's highly dynamic topology and time-varying link characteristics.The emulation results demonstrate the system's high reliability,and the user-space network stack reduces development complexity and debugging difficulty,providing convenience for the development of space network protocols and network functions.展开更多
Objective:To develop and demonstrate a comprehensive methodological framework for target trial emulation(TTE)implementation via post-laparoscopic septic shock fluid management as a clinical case study.Methods:We desig...Objective:To develop and demonstrate a comprehensive methodological framework for target trial emulation(TTE)implementation via post-laparoscopic septic shock fluid management as a clinical case study.Methods:We designed a simulated study of 10,000 patients in which colloid resuscitation was compared with crystalloid resuscitation.The framework incorporated precise eligibility criteria,standardized intervention protocols,and balanced treatment allocation with temporal anchoring.The primary analyses focused on 28-day mortality and were evaluated through survival analysis and comparative statistics.Results:The methodological framework successfully demonstrated the systematic implementation of TTE principles,providing reproducible protocols for eligibility determination,intervention standardization,and outcome assessment in observational studies.Conclusion:This work bridges the gap between TTE theory and practice by providing a detailed technical roadmap that enhances methodological transparency and reproducibility in comparative effectiveness research.展开更多
The proliferation of heterogeneous networks,such as the Internet of Things(IoT),unmanned aerial vehicle(UAV)networks,and edge networks,has increased the complexity of network operation and administration,driving the e...The proliferation of heterogeneous networks,such as the Internet of Things(IoT),unmanned aerial vehicle(UAV)networks,and edge networks,has increased the complexity of network operation and administration,driving the emergence of digital twin networks(DTNs)that create digital-physical network mappings.While DTNs enable performance analysis through emulation testbeds,current research focuses on network-level systems,neglecting equipment-level emulation of critical components like core switches and routers.To address this issue,we propose v Fabric(short for virtual switch),a digital twin emulator for high-capacity core switching equipment.This solution implements virtual switching and network processor(NP)chip models through specialized processes,deployable on single or distributed servers via socket communication.The v Fabric emulator can realize the accurate emulation for the core switching equipment with 720 ports and 100 Gbit/s per port on the largest scale.To our knowledge,this represents the first digital twin emulation framework specifically designed for large-capacity core switching equipment in communication networks.展开更多
Multi-organ-on-a-chip(MOOC)technology represents a pivotal direction in the organ-on-a-chip field,seeking to emulate the complex interactions of multiple human organs in vitro through microfluidic systems.This technol...Multi-organ-on-a-chip(MOOC)technology represents a pivotal direction in the organ-on-a-chip field,seeking to emulate the complex interactions of multiple human organs in vitro through microfluidic systems.This technology overcomes the limitations of traditional single-organ models,providing a novel platform for investigating complex disease mechanisms and evaluating drug efficacy and toxicity.Although it demonstrates broad application prospects,its development still faces critical bottlenecks,including inadequate physiological coupling between organs,short functional maintenance durations,and limited real-time monitoring capabilities.Contemporary research is advancing along three key directions,including functional coupling,sensor integration,and full-process automation systems,to propel the technology toward enhanced levels of physiological relevance and predictive accuracy.展开更多
As a promising solution to efficiently achieving fiber to the home （FTTH）, Ethernet passive optical network （EPON） is currently improved to provide intercommunication among customers, together with normal traffic ...As a promising solution to efficiently achieving fiber to the home （FTTH）, Ethernet passive optical network （EPON） is currently improved to provide intercommunication among customers, together with normal traffic delivery, via optical local area network emulation. It is a new research direction and expected to enhance the normal EPON performances. The purpose of this article is to review the state-of -the-art solutions to emulating optical local area networks （OLANs） over EPON. We discuss the major problems involved, e.g., network architecture, control mechanisms, and other potential enhancements. We also outline areas for future researches.展开更多
Robust-by-design(RbD)is a design strategy that uses adversary emulation to strengthen the security of an information and communication infrastructure.It relies on two key components:the security twin and the threat ac...Robust-by-design(RbD)is a design strategy that uses adversary emulation to strengthen the security of an information and communication infrastructure.It relies on two key components:the security twin and the threat actor twins.The security twin is a detailed database that outlines the different parts of the infrastructure,how they are connected,and their vulnerabilities.It also highlights the types of attacks each part could enable.On the other hand,the twin of a threat actor describes its potential attack surface,the attacks it can carry out,its strategy,and its ultimate goal,if any.This information comes from threat intelligence.RbD conducts independent simulations of various threat actors against the security twin to identify all possible attack paths they could exploit.Three types of analysis use this information to improve the robustness and resilience of the infrastructure.The first analysis fills in the gaps in threat intelligence by extending in-formation on threat actors and vulnerabilities.The second analysis focuses on selecting countermeasures aimed at eliminating attack paths or at least reducing their chances of success.Possible countermeasures include patching vulnerabilities,adjusting firewall rules,and implementing network segmentation.Information on attack paths guides the choice and configuration of these countermeasures.Once the infrastructure twin is updated to reflect countermeasure deployment,RbD performs further simulations to uncover any new attack paths that could be exploited and to identify additional coun-termeasures.The final analysis seeks to address the risks associated with any remaining unaddressed attack paths.展开更多
Based on the research on the solidification of twin-roll continuous casting aluminum thin strip, the analytical model of heterogeneous nucleation, the growth kinetics of tip （KGT） and columnar dendrite transformatio...Based on the research on the solidification of twin-roll continuous casting aluminum thin strip, the analytical model of heterogeneous nucleation, the growth kinetics of tip （KGT） and columnar dendrite transformation to equiaxed dendrite （CET） of twin-roll continuous casting aluminum thin strip solidification was established by means of the principle of metal solidification and modem computer emulational technology. Meantime, based on the cellular automaton, the emulational model of twin-roll continuous casting aluminum thin strip, solidification was established. The foundation for the emulational simulation of twin-roll casting thin strip solidification structure was laid. Meanwhile, the mathematical simulation feasibility was confirmed by using the solidification process of twin-roll continuous casting aluminum thin strip.展开更多
eMule是建立在点对点(peer to peer)技术上,以eDonkey协议为基础的文件共享软件。eMule的集中程度是混合性的,并非是完全无中心服务器。文章从协议的角度论述了eMule是如何实现文件共享,分析了客户端到服务器端、客户端到客户端之间的TC...eMule是建立在点对点(peer to peer)技术上,以eDonkey协议为基础的文件共享软件。eMule的集中程度是混合性的,并非是完全无中心服务器。文章从协议的角度论述了eMule是如何实现文件共享,分析了客户端到服务器端、客户端到客户端之间的TCP通讯、UDP通讯的原理及相关的信息序列图。展开更多
Based on the theory of random process and the characteristic of the functioning of a system component subject to a certain specific probability distribution, emulated the whole process of system production on Iongwall...Based on the theory of random process and the characteristic of the functioning of a system component subject to a certain specific probability distribution, emulated the whole process of system production on Iongwall face was emulated, in which the methods of time pace and computer random emulation were adopted. The paper provides an objective basis for the analysis of a mining Iongwall face and the varied lines of effectiveness, machine stoppage rate and coal flow of components and system, and output etc.lt also offers some theories to improve productivity by discussing the application of the transformation plan and optimization of reasonable coal stock capacity, as well as selection of system equipment and matching optimization.展开更多
文摘EMulate Therapeutics has developed a system for emulating the effects of solvated molecules via their magnetic field recordings. Recordings of magnetic field emissions of select small inhibitor RNAs (siRNAs;murine targeting CTLA-4 and murine targeting PD-1) were tested on C57Bl/6 mice implanted subcutaneously with the GL261 murine tumor cell line. A signal composed of concatenated recordings of siRNA molecules targeting the murine CTLA-4 and PD-1 receptors (labeled A2) was used in immune competent C57Bl/6 mice. The mice were flank implanted with the murine glioblastoma cell line GL261. Mice were exposed to the signal continuously (24 hours a day) until tumor volumes reached the designated volume limit. Tumors were excised and analyzed via PAGE/Western blot for the expression of CTLA-4, PD-1, Ki67, Caspase 3, CD4 and CD8. Terminal blood draws were used for CBCs. We report the down regulation of the checkpoint inhibitors CTLA-4 in the exposed mice. Significant tumor volume reduction was observed in mice exposed to the siRNA signal compared to control mice;no adverse events were recorded. Cell blood counts (CBC) and protein expression patterns were observed to correlate with the expected function of protein expression inhibition of the targets.
基金supported by project SERICS(PE00000014)under the MUR National Recovery and Resilience Plan funded by the European Union-NextGenerationEU.
文摘Blockchain,like any other complex technology,needs a strong testing methodology to support its evolution in both research and development contexts.Setting up meaningful tests for permissionless blockchain technology is a notoriously complex task for several reasons:software is complex,a large number of nodes are involved,the network is non-ideal,etc.Developers usually adopt small virtual laboratories or costly real devnets based on real software.Researchers usually prefer simulations of a large number of nodes based on simplified models.In this paper,we aim to obtain the advantages of both approaches,i.e.,performing large,realistic,inexpensive,and flexible experiments,using real blockchain software within a virtual environment.To do that,we address the challenge of running large blockchain networks in a single physical machine,leveraging Linux and Docker.We analyze a number of problems that arise when large blockchain networks are emulated,and we provide technical solutions for all of them.Finally,we describe two experiences of emulating fairly large blockchain networks on a single machine:adopting both research-oriented and production-oriented software and involving more than 3000 containers.
基金supported by the National Natural Science Foundation of China under Grant No.62131012ZTE Industry-University-Institute Cooperation Funds under Grant No.IA20230712005。
文摘This paper presents a space network emulation system based on a user-space network stack named Nos to solve space networks'unique architecture and routing issues and kernel stacks'inefficiency and development complexity.Our low Earth orbit satellite scenario emulation verifies the dynamic routing function of the protocol stack.The proposed system uses technologies like Open vSwitch(OVS)and traffic control(TC)to emulate the space network's highly dynamic topology and time-varying link characteristics.The emulation results demonstrate the system's high reliability,and the user-space network stack reduces development complexity and debugging difficulty,providing convenience for the development of space network protocols and network functions.
基金Zhongheng Zhang received funding from the National Natural Science Foundation of China(82272180,82472243)the China National Key Research and Development Program(2023YFC3603104)+5 种基金the Huadong Medicine Joint Funds of the Zhejiang Provincial Natural Science Foundation of China(LHDMD24H150001)the China National Key Research and Development Program(2022YFC2504500)a collaborative scientific project co-established by the Science and Technology Department of the National Administration of Traditional Chinese Medicine and the Zhejiang Provincial Administration of Traditional Chinese Medicine(GZY-ZJKJ-24082)the General Health Science and Technology Program of Zhejiang Province(2024KY1099)Project of Zhejiang University Longquan Innovation Center(ZJDXLQCXZCJBGS2024016)Hongying Ni received funding from Jinhua Science and Technology Planning Project(2024-3-047).
文摘Objective:To develop and demonstrate a comprehensive methodological framework for target trial emulation(TTE)implementation via post-laparoscopic septic shock fluid management as a clinical case study.Methods:We designed a simulated study of 10,000 patients in which colloid resuscitation was compared with crystalloid resuscitation.The framework incorporated precise eligibility criteria,standardized intervention protocols,and balanced treatment allocation with temporal anchoring.The primary analyses focused on 28-day mortality and were evaluated through survival analysis and comparative statistics.Results:The methodological framework successfully demonstrated the systematic implementation of TTE principles,providing reproducible protocols for eligibility determination,intervention standardization,and outcome assessment in observational studies.Conclusion:This work bridges the gap between TTE theory and practice by providing a detailed technical roadmap that enhances methodological transparency and reproducibility in comparative effectiveness research.
基金supported in part by the National Natural Science Foundation of China(NSFC)under Grant Nos.62171085,62272428,62001087,U20A20156,and 61871097the ZTE Industry-University-Institute Cooperation Funds under Grant No.HC-CN-20220722010。
文摘The proliferation of heterogeneous networks,such as the Internet of Things(IoT),unmanned aerial vehicle(UAV)networks,and edge networks,has increased the complexity of network operation and administration,driving the emergence of digital twin networks(DTNs)that create digital-physical network mappings.While DTNs enable performance analysis through emulation testbeds,current research focuses on network-level systems,neglecting equipment-level emulation of critical components like core switches and routers.To address this issue,we propose v Fabric(short for virtual switch),a digital twin emulator for high-capacity core switching equipment.This solution implements virtual switching and network processor(NP)chip models through specialized processes,deployable on single or distributed servers via socket communication.The v Fabric emulator can realize the accurate emulation for the core switching equipment with 720 ports and 100 Gbit/s per port on the largest scale.To our knowledge,this represents the first digital twin emulation framework specifically designed for large-capacity core switching equipment in communication networks.
基金supported by the Shenzhen Medical Research Fund(Grant No.A2303049)Guangdong Basic and Applied Basic Research(Grant No.2023A1515010647)+1 种基金National Natural Science Foundation of China(Grant No.22004135)Shenzhen Science and Technology Program(Grant No.RCBS20210706092409020,GXWD20201231165807008,20200824162253002).
文摘Multi-organ-on-a-chip(MOOC)technology represents a pivotal direction in the organ-on-a-chip field,seeking to emulate the complex interactions of multiple human organs in vitro through microfluidic systems.This technology overcomes the limitations of traditional single-organ models,providing a novel platform for investigating complex disease mechanisms and evaluating drug efficacy and toxicity.Although it demonstrates broad application prospects,its development still faces critical bottlenecks,including inadequate physiological coupling between organs,short functional maintenance durations,and limited real-time monitoring capabilities.Contemporary research is advancing along three key directions,including functional coupling,sensor integration,and full-process automation systems,to propel the technology toward enhanced levels of physiological relevance and predictive accuracy.
基金the National Science Fund for Distinguished Young Scholars(Grant No.60725104)the National Basic Research Program of China(Grant No.2007CB310706)+2 种基金the National High Technology Research and Development Program of China(Grant Nos.2007AA01Z246 and 2007AA01Z227)the National Natural Science Foundation of China(Grant No.60672045)the Research Fund for the Doctoral Program of Higher Education(Grant No.20060614018)
文摘As a promising solution to efficiently achieving fiber to the home （FTTH）, Ethernet passive optical network （EPON） is currently improved to provide intercommunication among customers, together with normal traffic delivery, via optical local area network emulation. It is a new research direction and expected to enhance the normal EPON performances. The purpose of this article is to review the state-of -the-art solutions to emulating optical local area networks （OLANs） over EPON. We discuss the major problems involved, e.g., network architecture, control mechanisms, and other potential enhancements. We also outline areas for future researches.
文摘Robust-by-design(RbD)is a design strategy that uses adversary emulation to strengthen the security of an information and communication infrastructure.It relies on two key components:the security twin and the threat actor twins.The security twin is a detailed database that outlines the different parts of the infrastructure,how they are connected,and their vulnerabilities.It also highlights the types of attacks each part could enable.On the other hand,the twin of a threat actor describes its potential attack surface,the attacks it can carry out,its strategy,and its ultimate goal,if any.This information comes from threat intelligence.RbD conducts independent simulations of various threat actors against the security twin to identify all possible attack paths they could exploit.Three types of analysis use this information to improve the robustness and resilience of the infrastructure.The first analysis fills in the gaps in threat intelligence by extending in-formation on threat actors and vulnerabilities.The second analysis focuses on selecting countermeasures aimed at eliminating attack paths or at least reducing their chances of success.Possible countermeasures include patching vulnerabilities,adjusting firewall rules,and implementing network segmentation.Information on attack paths guides the choice and configuration of these countermeasures.Once the infrastructure twin is updated to reflect countermeasure deployment,RbD performs further simulations to uncover any new attack paths that could be exploited and to identify additional coun-termeasures.The final analysis seeks to address the risks associated with any remaining unaddressed attack paths.
基金Project (u0837601) supported by the New Joint Fund of National Natural Science Foundation of ChinaProject (50874054) supported by the National Natural Science Foundation of China
文摘Based on the research on the solidification of twin-roll continuous casting aluminum thin strip, the analytical model of heterogeneous nucleation, the growth kinetics of tip （KGT） and columnar dendrite transformation to equiaxed dendrite （CET） of twin-roll continuous casting aluminum thin strip solidification was established by means of the principle of metal solidification and modem computer emulational technology. Meantime, based on the cellular automaton, the emulational model of twin-roll continuous casting aluminum thin strip, solidification was established. The foundation for the emulational simulation of twin-roll casting thin strip solidification structure was laid. Meanwhile, the mathematical simulation feasibility was confirmed by using the solidification process of twin-roll continuous casting aluminum thin strip.
文摘eMule是建立在点对点(peer to peer)技术上,以eDonkey协议为基础的文件共享软件。eMule的集中程度是混合性的,并非是完全无中心服务器。文章从协议的角度论述了eMule是如何实现文件共享,分析了客户端到服务器端、客户端到客户端之间的TCP通讯、UDP通讯的原理及相关的信息序列图。
基金Supported by the Special Topics of Major Scientific and Technological Research Projects Shanxi Province (2006031099-01)Youth Science Funds of Shanxi Province (2006021024)Science and Technology Project of Taiyuan City (07010728)
文摘Based on the theory of random process and the characteristic of the functioning of a system component subject to a certain specific probability distribution, emulated the whole process of system production on Iongwall face was emulated, in which the methods of time pace and computer random emulation were adopted. The paper provides an objective basis for the analysis of a mining Iongwall face and the varied lines of effectiveness, machine stoppage rate and coal flow of components and system, and output etc.lt also offers some theories to improve productivity by discussing the application of the transformation plan and optimization of reasonable coal stock capacity, as well as selection of system equipment and matching optimization.
