Each stage of embryonic development,including normal gamete maturation,fertilization,zygotic genome activation,and cleavage,is crucial for human reproduction.Early embryo arrest is a common phenomenon.It is estimated ...Each stage of embryonic development,including normal gamete maturation,fertilization,zygotic genome activation,and cleavage,is crucial for human reproduction.Early embryo arrest is a common phenomenon.It is estimated that about 40%–70%of human embryos are arrested at early developmental stages.However,the exact mechanism remains largely uncertain.Embryos can be investigated in vitro by way of the development of in vitro fertilization/intracytoplasmic sperm injection.In addition to iatrogenic factors related to abnormal oocyte/embryo development,multiple gene mutations have been found to be involved in such phenotypes.Based on the knowledge of known etiological factors,several therapies are proposed to improve clinical outcomes.Here,we shed light on current and potential therapies for treating these conditions through reviewing articles and combining with our clinical and research experience.展开更多
To investigate the relationship of the variation of virulence and the external capsid proteins of the pandemic duck hepatitis A virus type 1(DHAV-1) isolates,the virulence,cross neutralization assays and the complete ...To investigate the relationship of the variation of virulence and the external capsid proteins of the pandemic duck hepatitis A virus type 1(DHAV-1) isolates,the virulence,cross neutralization assays and the complete sequence of the virion protein 1(VP1) gene of nine virulent DHAV-1 strains,which were isolated from infected ducklings with clinical symptoms in Shandong province of China in 2007-2008,were tested.The fifth generation duck embryo allantoic liquids of the 9 isolates were tested on 12-day-old duck embryos and on 7-day-old ducklings for the median embryonal lethal doses(ELD 50 s) and the median lethal doses(LD 50 s),respectively.The results showed that the ELD 50 s of embryonic duck eggs of the 9 DHAV-1 isolates were between 1.9 × 10 6 /mL to 1.44 × 10 7 /mL,while the LD 50 s were 2.39 × 10 5 /mL to 6.15 × 10 6 /mL.Cross-neutralization tests revealed that the 9 DHAV-1 isolates were completely neutralized by the standard serum and the hyperimmune sera against the 9 DHAV-1 isolates,respectively.Compared with other virulent,moderate virulent,attenuated vaccine and mild strains,the VP1 genes of the 9 strains shared 89.8%-99.7% similarity at the nucleotide level and 92.4%-99.6% at amino acid level with other DHAV-1 strains.There were three hypervariable regions at the C-terminus(aa 158-160,180-193 and 205-219) and other variable points in VP1 protein,but which didn't cause virulence of DHAV-1 change.展开更多
Dear Editor,Large-scale phenotyping efforts have shown that placental defects correlate strongly with embryonic lethal mouse mutants(Perez-Garcia et al.,2018).To rescue normal development of these mutant embryos,tetra...Dear Editor,Large-scale phenotyping efforts have shown that placental defects correlate strongly with embryonic lethal mouse mutants(Perez-Garcia et al.,2018).To rescue normal development of these mutant embryos,tetraploid complementation can be a reliable strategy,in which,tetraploid cells form extraembryonic lineages thus providing the embryo with a wild-type placenta(Nagy et al.,1990).展开更多
基金This project was supported by The Third Batch of China Post-Doctoral Special Funding(Grant 2021TQ0372 to H.L.)the Key Research and Development Plan of Hunan Province(Grant 2021SK2028 to H.L.).
文摘Each stage of embryonic development,including normal gamete maturation,fertilization,zygotic genome activation,and cleavage,is crucial for human reproduction.Early embryo arrest is a common phenomenon.It is estimated that about 40%–70%of human embryos are arrested at early developmental stages.However,the exact mechanism remains largely uncertain.Embryos can be investigated in vitro by way of the development of in vitro fertilization/intracytoplasmic sperm injection.In addition to iatrogenic factors related to abnormal oocyte/embryo development,multiple gene mutations have been found to be involved in such phenotypes.Based on the knowledge of known etiological factors,several therapies are proposed to improve clinical outcomes.Here,we shed light on current and potential therapies for treating these conditions through reviewing articles and combining with our clinical and research experience.
基金the Chinese National Natural Sciences Foundation(30871878)Shandong Province Higher Educational Science and Technology Program(J08LF07)the Science and Technology Commission of Shandong Province(2010GNC10914),China
文摘To investigate the relationship of the variation of virulence and the external capsid proteins of the pandemic duck hepatitis A virus type 1(DHAV-1) isolates,the virulence,cross neutralization assays and the complete sequence of the virion protein 1(VP1) gene of nine virulent DHAV-1 strains,which were isolated from infected ducklings with clinical symptoms in Shandong province of China in 2007-2008,were tested.The fifth generation duck embryo allantoic liquids of the 9 isolates were tested on 12-day-old duck embryos and on 7-day-old ducklings for the median embryonal lethal doses(ELD 50 s) and the median lethal doses(LD 50 s),respectively.The results showed that the ELD 50 s of embryonic duck eggs of the 9 DHAV-1 isolates were between 1.9 × 10 6 /mL to 1.44 × 10 7 /mL,while the LD 50 s were 2.39 × 10 5 /mL to 6.15 × 10 6 /mL.Cross-neutralization tests revealed that the 9 DHAV-1 isolates were completely neutralized by the standard serum and the hyperimmune sera against the 9 DHAV-1 isolates,respectively.Compared with other virulent,moderate virulent,attenuated vaccine and mild strains,the VP1 genes of the 9 strains shared 89.8%-99.7% similarity at the nucleotide level and 92.4%-99.6% at amino acid level with other DHAV-1 strains.There were three hypervariable regions at the C-terminus(aa 158-160,180-193 and 205-219) and other variable points in VP1 protein,but which didn't cause virulence of DHAV-1 change.
基金supported by the Genome Tagging Project and grants from the National Key Research and Development Program of China(2024YFA1800010,2020YFA0509000)the National Science and Technology Major Project of China(2023ZD0500500)+2 种基金the National Natural Science Foundation of China(32488101,32030029,32293231,32370909)partly supported by Shanghai Municipal Science and Technology Major Project(22YS1400900,23HC1401000)support of the Sanofi Scholarship Program.
文摘Dear Editor,Large-scale phenotyping efforts have shown that placental defects correlate strongly with embryonic lethal mouse mutants(Perez-Garcia et al.,2018).To rescue normal development of these mutant embryos,tetraploid complementation can be a reliable strategy,in which,tetraploid cells form extraembryonic lineages thus providing the embryo with a wild-type placenta(Nagy et al.,1990).
