The Dendrobium kingianum is a highly valued ornamental plant belonging to the Orchidaceae family whose propagation relies on time-consuming stem cutting and protocorm-like body proliferation.To optimize its propagatio...The Dendrobium kingianum is a highly valued ornamental plant belonging to the Orchidaceae family whose propagation relies on time-consuming stem cutting and protocorm-like body proliferation.To optimize its propagation system,roots,leaves,and lateral buds of Dendrobium kingianum plants were taken to investigate embryogenic callus induction and somatic embryogenesis.Embryogenic callus was induced on MS medium containing 0.2 mg/L NAA,1.5 mg/L 6-BA,and 10−7 mol/L PSK when lateral buds served as explants.Many somatic embryos were generated on G4 medium(1.0 g/L Hyponex No.1+1.0 g/L Hyponex No.2+15 g/L sucrose+3 g/L Phytagel+2 mg/L 6-BA+0.5 mg/L IBA+10 g/L corn starch+200 mL/L coconut water),which developed into robust seedlings after rooting.The original embryogenic calluses proliferated continuously for more than half a year,and the newly proliferated ones possessed the capacity to develop into seedlings,indicating an efficient propagation system for Dendrobium kingianum via somatic embryogenesis with potential for scale production.展开更多
基金supported by Yunnan Xingdian Talents—Youth Special Project(Grant No.XDYC-QNRC-2022-0731)Yunnan Seed Laboratory Project(Grant No.202205AR070001-2024KF01)Yunnan Xingdian Talents—Special Selection Project for High-level Scientific and Technological Talents and Innovation Teams-Team Specific Project,Xingdian Talent Support Project(Grant No.CYRC2020004).
文摘The Dendrobium kingianum is a highly valued ornamental plant belonging to the Orchidaceae family whose propagation relies on time-consuming stem cutting and protocorm-like body proliferation.To optimize its propagation system,roots,leaves,and lateral buds of Dendrobium kingianum plants were taken to investigate embryogenic callus induction and somatic embryogenesis.Embryogenic callus was induced on MS medium containing 0.2 mg/L NAA,1.5 mg/L 6-BA,and 10−7 mol/L PSK when lateral buds served as explants.Many somatic embryos were generated on G4 medium(1.0 g/L Hyponex No.1+1.0 g/L Hyponex No.2+15 g/L sucrose+3 g/L Phytagel+2 mg/L 6-BA+0.5 mg/L IBA+10 g/L corn starch+200 mL/L coconut water),which developed into robust seedlings after rooting.The original embryogenic calluses proliferated continuously for more than half a year,and the newly proliferated ones possessed the capacity to develop into seedlings,indicating an efficient propagation system for Dendrobium kingianum via somatic embryogenesis with potential for scale production.
