The rates of oxygen consumption, tolerance of hypoxia and desiccation of the Chinese black sleeper （ Bostrichthys sinensis） and mudskipper （Boleophthalmus pectinirostris） embryos were investigated. The pattern of ...The rates of oxygen consumption, tolerance of hypoxia and desiccation of the Chinese black sleeper （ Bostrichthys sinensis） and mudskipper （Boleophthalmus pectinirostris） embryos were investigated. The pattern of oxygen consumption of the Chinese black sleeper embryos was similar to that of the mudskipper ones. The lowest rates of oxygen consumption [ （ 1.65 ±0. 66） nmol/ （ind. ·h） ] of the Chinese black sleeper embryos 16 h after fertilization and the lowest rates of oxygen consumption [ （0.79± 0.08 ）nmolf（ ind. · h） ] of the mudskipper embryos 6 h after fertilization were recorded, respectively. Then the rates of oxygen consumption of these two species embryos increased gradually until hatching [ （8.26 ± 1.70 ） nmolf（ ind.· h） in the Chinese black sleeper, （2.69 ± 0.23 ）nmolf（ ind. · h） in mudskipper]. After exposure to hypoxia water （0.16 mg/dm^3）, bradycardia of the embryos occurred in both the Chinese black sleeper and the mudskipper. However, the Chinese black sleeper embryos survived approximately 45 min longer than the mudskipper ones. After exposure to desiccation at a relative humidity of 58%, bradycardia of the embryos was observed in both the Chinese black sleeper and the mudskipper, and the Chinese black sleeper embryos lived approximately 9 min longer than the mudskipper ones.展开更多
Objective To analyse factors influencing the outcome of frozen-thawed embryo transfer （FET）. Method A retrospective analysis was performed in our center on 129 thawing cycles from March 2001 to April 2003. The relat...Objective To analyse factors influencing the outcome of frozen-thawed embryo transfer （FET）. Method A retrospective analysis was performed in our center on 129 thawing cycles from March 2001 to April 2003. The related parameters were compared between conceived and non-conceived cycles. Results There were totally 129 clinical pregnancies in these transfers （pregnancy rate： 27.1%）. Frozen-thawed embryos were transferred to natural cycles and CC cycling and hormone replacement treatment had equal success. Groups of IVF and ICSI did not differ significantly in pregnancy rates （P〉0.05）. The pregnancy rates for one, two, three and four pre-embryos transfer were 0, 20.0%,44.1% and 75.0%, respectively （P〈0.05）. There were statistical differences between pregnancy group or non- pregnancy group in the endometrial thickness, CES, CES/No. of embryo. A higher pregnancy rate was observed in embryo transfers which had at least one 4-cell grade I embryo （d 2）（P〈0.01）. Conclusions The most important factors influencing the implantation rate and pregnancy rate of frozen-thawed embryo transfer are age, endometrium thickness, and the number, morphology and growth rate of transferred frozen embryos of women participants.展开更多
【目的】奶牛体外胚胎生产-移植(in vitro embryo production and embryo transfer,IVP-ET)是现代奶牛养殖的重要繁殖手段之一。为解析奶牛体外胚胎移植受胎率的影响因素,本研究利用大规模数据分析了荷斯坦牛体外胚胎移植受胎率的表型特...【目的】奶牛体外胚胎生产-移植(in vitro embryo production and embryo transfer,IVP-ET)是现代奶牛养殖的重要繁殖手段之一。为解析奶牛体外胚胎移植受胎率的影响因素,本研究利用大规模数据分析了荷斯坦牛体外胚胎移植受胎率的表型特征,为优化体外胚胎生产移植技术体系提供可用信息。【方法】本研究收集了11个规模化奶牛场4534头荷斯坦牛的5155条体外胚胎移植记录及其对应的孕检记录,采用Logistic回归模型分析各非遗传因素(如胚胎因素等)对奶牛体外胚胎移植受胎率的影响。【结果】荷斯坦青年牛体外胚胎移植总体受胎率为51.29%。胚胎因素(保存状态、发育阶段、体外培养天数)及受体牛移植次数对移植受胎率有极显著影响,其中,移植新鲜胚胎的受胎率极显著高于冷冻胚胎(P<0.01);不同发育阶段的胚胎中,发育至扩张囊胚的受胎率极显著高于囊胚(P<0.01);体外培养天数不同的胚胎中,培养至第6天下午、第7天上午及下午的胚胎的移植受胎率均极显著高于培养至第8天下午的胚胎(P<0.01);首次移植的受胎率极显著高于非首次移植(P<0.01)。受体牛月龄和胚胎父本对移植受胎率有显著影响,其中>14.5且≤15月龄受体牛的受胎率最高(52.59%),且极显著高于<14月龄的受体牛(P<0.01);胚胎父本显著影响移植受胎率(P<0.05),表明由父本决定的胚胎内在质量是影响移植成功与否的关键生产端因素,进一步提示胚胎生产与胚胎移植息息相关。【结论】基于大规模胚胎移植记录,本研究发现胚胎保存状态、发育阶段、体外培养天数及受体牛移植次数对移植受胎率有显著影响,优化胚胎质量与选择适宜受体是提高荷斯坦牛体外胚胎移植受胎率的关键。展开更多
Objective To investigate the effect of FTY720-treated immature bone marrow-derived dendritic cells（BMDCs） on the embryo resorption rate in the CBA/J× DBA/2 abortion mouse model.Methods The dendritic cells（DCs...Objective To investigate the effect of FTY720-treated immature bone marrow-derived dendritic cells（BMDCs） on the embryo resorption rate in the CBA/J× DBA/2 abortion mouse model.Methods The dendritic cells（DCs） were derived from bone marrow of DBA/2 mice, and then co-cultured with FTY720. The abortion mouse models were established by mating female CBA/J mice with DBA/2 mice. Via the CBA/J×DBA/2 abortion mouse model, six groups were established, group A： normal pregnancy model; group B： abortion mouse model with no treatment; group C： abortion mouse model injected with DC culture medium（DCCM）; group D： abortion mouse model injected with DC; group E： abortion mouse model injected with FTY720; group F： abortion model mouse injected with FTY720-DC. The differences were compared in the embryo resorption rates of the CBA/J ×DBA/2 abortion mouse model treated with FTY720-DC or different controls observed on gestation day 12 to 14, and then the microenvironment in murine pregnancy was investigated.Results The embryo resorption rate was statistically significantly decreased in group D and group E when they compared with group B and group C（P〈0.05, respectively）.Furthermore, the embryo resorption rate in group F showed a statistically significant decrease when compared with the other groups except group A（P〈0.01）. These resultssuggest that FTY720-DCs possess a notable advantage over DCs or FTY720 in reducing the embryo resorption rate of the abortion mouse model. The percentage of Th17（IL-17＋CD4＋T cells） in peripheral blood mononuclear cell（PBMC） in the abortion mouse model was 4.35%±0.34% before treated with FTY720-DC, and was1.34%±0.28% after treated with FTY720-DC（P〈0.01）. The percentage of Tregs（CD4~＋CD25~＋Foxp3~＋T cells） in PBMC was significantly increased in group F（8.35%±1.80%） as compared with group B（2.68%±0.65%）（P〈0.01）.Conclusion Adoptive transfer of FTY720-DC can statistically significantly reduce the embryo resorption rate in the CBA/J×DBA/2 abortion mouse model. The lower embryo resorption rate in the FTY720-DC treated abortion mouse model may be caused by the imbalance of Treg/Th17.展开更多
The structures of mature embryo sacs in 13 genetic stock lines of autotetraploid rice （Oryza sativa L.）, including indica, japonica and javanica, were studied by using the whole-mount stain-clearing laser scanning c...The structures of mature embryo sacs in 13 genetic stock lines of autotetraploid rice （Oryza sativa L.）, including indica, japonica and javanica, were studied by using the whole-mount stain-clearing laser scanning confocal microscopy （WCLSM）. Among the 13 autotetraploid rice, the majority of ovaries possess normal polygonum-type embryo sacs, while a few ovaries were characterized by abnormal embryo sacs. The abnormalities of embryo sacs could be classified into six categories, i. e. no female germ unit, abnormal polar nuclei, embryo sac degeneration, no egg apparatus, small embryo sac and ＇double set＇ of embryo sacs. The frequency of abnormal embryo sac in japonica （26.6%） was higher than that in indica （19.34%）. In addition, the major abnormalities in each autotetraploid line varied, suggesting that the abnormalities may be related to the genotypes of the varieties.展开更多
基金supported by the National Natural Science Foundation of China under contract No.40476056the Science and Technology Key Project of Fujian Province of China under contract Nos 2003N026 and 2004SZ01-02.
文摘The rates of oxygen consumption, tolerance of hypoxia and desiccation of the Chinese black sleeper （ Bostrichthys sinensis） and mudskipper （Boleophthalmus pectinirostris） embryos were investigated. The pattern of oxygen consumption of the Chinese black sleeper embryos was similar to that of the mudskipper ones. The lowest rates of oxygen consumption [ （ 1.65 ±0. 66） nmol/ （ind. ·h） ] of the Chinese black sleeper embryos 16 h after fertilization and the lowest rates of oxygen consumption [ （0.79± 0.08 ）nmolf（ ind. · h） ] of the mudskipper embryos 6 h after fertilization were recorded, respectively. Then the rates of oxygen consumption of these two species embryos increased gradually until hatching [ （8.26 ± 1.70 ） nmolf（ ind.· h） in the Chinese black sleeper, （2.69 ± 0.23 ）nmolf（ ind. · h） in mudskipper]. After exposure to hypoxia water （0.16 mg/dm^3）, bradycardia of the embryos occurred in both the Chinese black sleeper and the mudskipper. However, the Chinese black sleeper embryos survived approximately 45 min longer than the mudskipper ones. After exposure to desiccation at a relative humidity of 58%, bradycardia of the embryos was observed in both the Chinese black sleeper and the mudskipper, and the Chinese black sleeper embryos lived approximately 9 min longer than the mudskipper ones.
文摘Objective To analyse factors influencing the outcome of frozen-thawed embryo transfer （FET）. Method A retrospective analysis was performed in our center on 129 thawing cycles from March 2001 to April 2003. The related parameters were compared between conceived and non-conceived cycles. Results There were totally 129 clinical pregnancies in these transfers （pregnancy rate： 27.1%）. Frozen-thawed embryos were transferred to natural cycles and CC cycling and hormone replacement treatment had equal success. Groups of IVF and ICSI did not differ significantly in pregnancy rates （P〉0.05）. The pregnancy rates for one, two, three and four pre-embryos transfer were 0, 20.0%,44.1% and 75.0%, respectively （P〈0.05）. There were statistical differences between pregnancy group or non- pregnancy group in the endometrial thickness, CES, CES/No. of embryo. A higher pregnancy rate was observed in embryo transfers which had at least one 4-cell grade I embryo （d 2）（P〈0.01）. Conclusions The most important factors influencing the implantation rate and pregnancy rate of frozen-thawed embryo transfer are age, endometrium thickness, and the number, morphology and growth rate of transferred frozen embryos of women participants.
文摘【目的】奶牛体外胚胎生产-移植(in vitro embryo production and embryo transfer,IVP-ET)是现代奶牛养殖的重要繁殖手段之一。为解析奶牛体外胚胎移植受胎率的影响因素,本研究利用大规模数据分析了荷斯坦牛体外胚胎移植受胎率的表型特征,为优化体外胚胎生产移植技术体系提供可用信息。【方法】本研究收集了11个规模化奶牛场4534头荷斯坦牛的5155条体外胚胎移植记录及其对应的孕检记录,采用Logistic回归模型分析各非遗传因素(如胚胎因素等)对奶牛体外胚胎移植受胎率的影响。【结果】荷斯坦青年牛体外胚胎移植总体受胎率为51.29%。胚胎因素(保存状态、发育阶段、体外培养天数)及受体牛移植次数对移植受胎率有极显著影响,其中,移植新鲜胚胎的受胎率极显著高于冷冻胚胎(P<0.01);不同发育阶段的胚胎中,发育至扩张囊胚的受胎率极显著高于囊胚(P<0.01);体外培养天数不同的胚胎中,培养至第6天下午、第7天上午及下午的胚胎的移植受胎率均极显著高于培养至第8天下午的胚胎(P<0.01);首次移植的受胎率极显著高于非首次移植(P<0.01)。受体牛月龄和胚胎父本对移植受胎率有显著影响,其中>14.5且≤15月龄受体牛的受胎率最高(52.59%),且极显著高于<14月龄的受体牛(P<0.01);胚胎父本显著影响移植受胎率(P<0.05),表明由父本决定的胚胎内在质量是影响移植成功与否的关键生产端因素,进一步提示胚胎生产与胚胎移植息息相关。【结论】基于大规模胚胎移植记录,本研究发现胚胎保存状态、发育阶段、体外培养天数及受体牛移植次数对移植受胎率有显著影响,优化胚胎质量与选择适宜受体是提高荷斯坦牛体外胚胎移植受胎率的关键。
基金supported by the National Natural Science Foundation of China(Grant No.81200477)
文摘Objective To investigate the effect of FTY720-treated immature bone marrow-derived dendritic cells（BMDCs） on the embryo resorption rate in the CBA/J× DBA/2 abortion mouse model.Methods The dendritic cells（DCs） were derived from bone marrow of DBA/2 mice, and then co-cultured with FTY720. The abortion mouse models were established by mating female CBA/J mice with DBA/2 mice. Via the CBA/J×DBA/2 abortion mouse model, six groups were established, group A： normal pregnancy model; group B： abortion mouse model with no treatment; group C： abortion mouse model injected with DC culture medium（DCCM）; group D： abortion mouse model injected with DC; group E： abortion mouse model injected with FTY720; group F： abortion model mouse injected with FTY720-DC. The differences were compared in the embryo resorption rates of the CBA/J ×DBA/2 abortion mouse model treated with FTY720-DC or different controls observed on gestation day 12 to 14, and then the microenvironment in murine pregnancy was investigated.Results The embryo resorption rate was statistically significantly decreased in group D and group E when they compared with group B and group C（P〈0.05, respectively）.Furthermore, the embryo resorption rate in group F showed a statistically significant decrease when compared with the other groups except group A（P〈0.01）. These resultssuggest that FTY720-DCs possess a notable advantage over DCs or FTY720 in reducing the embryo resorption rate of the abortion mouse model. The percentage of Th17（IL-17＋CD4＋T cells） in peripheral blood mononuclear cell（PBMC） in the abortion mouse model was 4.35%±0.34% before treated with FTY720-DC, and was1.34%±0.28% after treated with FTY720-DC（P〈0.01）. The percentage of Tregs（CD4~＋CD25~＋Foxp3~＋T cells） in PBMC was significantly increased in group F（8.35%±1.80%） as compared with group B（2.68%±0.65%）（P〈0.01）.Conclusion Adoptive transfer of FTY720-DC can statistically significantly reduce the embryo resorption rate in the CBA/J×DBA/2 abortion mouse model. The lower embryo resorption rate in the FTY720-DC treated abortion mouse model may be caused by the imbalance of Treg/Th17.
基金supported by the National Natural Science Foundations of China(Grant No.30270814)the Teaching and Research Award Program for 0utstanding Young Teachers in Higher Education Institutions of M0E,P.R.China.
文摘The structures of mature embryo sacs in 13 genetic stock lines of autotetraploid rice （Oryza sativa L.）, including indica, japonica and javanica, were studied by using the whole-mount stain-clearing laser scanning confocal microscopy （WCLSM）. Among the 13 autotetraploid rice, the majority of ovaries possess normal polygonum-type embryo sacs, while a few ovaries were characterized by abnormal embryo sacs. The abnormalities of embryo sacs could be classified into six categories, i. e. no female germ unit, abnormal polar nuclei, embryo sac degeneration, no egg apparatus, small embryo sac and ＇double set＇ of embryo sacs. The frequency of abnormal embryo sac in japonica （26.6%） was higher than that in indica （19.34%）. In addition, the major abnormalities in each autotetraploid line varied, suggesting that the abnormalities may be related to the genotypes of the varieties.
