[Objectives]This study was conducted to provide a new way for the rapid reproduction of Symplocos paniculata.[Methods]With mature embryos of S.paniculata as explants,through the study of somatic embryogenesis,the effe...[Objectives]This study was conducted to provide a new way for the rapid reproduction of Symplocos paniculata.[Methods]With mature embryos of S.paniculata as explants,through the study of somatic embryogenesis,the effects of different media on the induction and differentiation of S.paniculata somatic embryos were discussed,and the somatic embryogenesis system for S.paniculata was preliminarily established.[Results]Calli suitable for somatic embryogenesis could be induced using modified MS(referred to as GMS)as the basic medium with the addition of 0.2 mg/L 6-BA and 0.1 mg/L NAA;the best medium combination for somatic embryo differentiation was GMS+0.25 mg/L 6-BA+0.15 mg/L NAA,and the differentiation rate could reach 71.99%;and plant regeneration needed to be carried out at low sugar concentrations.[Conclusions]This study provides a technical basis for resources protection,genetic transformation,artificial seed production,and large-scale development and utilization of S.paniculata.展开更多
基金Supported by Changsha Science and Technology Plan Key Project(kq1801080)State Key Laboratory of Woody Oil Resource Utilization Project(2019XK2002)。
文摘[Objectives]This study was conducted to provide a new way for the rapid reproduction of Symplocos paniculata.[Methods]With mature embryos of S.paniculata as explants,through the study of somatic embryogenesis,the effects of different media on the induction and differentiation of S.paniculata somatic embryos were discussed,and the somatic embryogenesis system for S.paniculata was preliminarily established.[Results]Calli suitable for somatic embryogenesis could be induced using modified MS(referred to as GMS)as the basic medium with the addition of 0.2 mg/L 6-BA and 0.1 mg/L NAA;the best medium combination for somatic embryo differentiation was GMS+0.25 mg/L 6-BA+0.15 mg/L NAA,and the differentiation rate could reach 71.99%;and plant regeneration needed to be carried out at low sugar concentrations.[Conclusions]This study provides a technical basis for resources protection,genetic transformation,artificial seed production,and large-scale development and utilization of S.paniculata.
