To investigate the contribution of fibre arrangement to guiding the aligned growth of corneal stroma cells,aligned and randomly oriented fibrous scaffolds of gelatin and poly-L-lactic acid（PLLA） were fabricated by e...To investigate the contribution of fibre arrangement to guiding the aligned growth of corneal stroma cells,aligned and randomly oriented fibrous scaffolds of gelatin and poly-L-lactic acid（PLLA） were fabricated by electrospinning.A comparative study of two different systems with corneal stroma cells on randomly organized and aligned fibres were conducted.The efficiency of the scaffolds for inducing the aligned growth of cells was assessed by morphological observation and 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl-tetrazolium bromide（MTT） assay.Results show that the cells cultured on both randomly oriented and aligned scaffolds maintained normal morphology and well spreading as well as long term proliferation.Importantly,corneal stroma cells grew high orderly on the aligned scaffold,while the cells grew disordered on the randomly oriented scaffold.Moreover,the cells exhibited higher viability in aligned scaffold than that in randomly oriented scaffold.These results indcate that electrospinng to prepare aligned fibrous scaffolds has provided an effective approach to the aligned growth of corneal stroma cells in vitro.Our findings that fiber arrangement plays a crucial role in guiding the aligned growth of cells may be helpful to the development of better biomaterials for tissue engineered cornea.展开更多
Composite nanofibrous mats consisting of poly( L-lactideco-ε-caprolactone)( PLCL) and collagen type I( COL) were fabricated by electrospinning,and ten times simulated body fluid(10SBF) were employed to mineralize nan...Composite nanofibrous mats consisting of poly( L-lactideco-ε-caprolactone)( PLCL) and collagen type I( COL) were fabricated by electrospinning,and ten times simulated body fluid(10SBF) were employed to mineralize nanofibrous mats. Ballshaped hydroxyapatite( HA) was deposited on the surface of nanofibrous mats in 1. 5 h at room temperature. Human fetal osteoblasts( hFob) were seeded to investigate their proliferation and differentiation on mineralized composite nanofibrous mats. The results showed that hFob grew well on mineralized composite nanofibrous mats and alkaline phosphatase( ALP) activity of hFob on mineralized composite nanofibrous mats at 14 d was much higher than that on untreated nanofibrous mats. Moreover,the expression of osteocalcin of cells on mineralized composite nanofibrous mats was also much higher than those on untreated nanofibrous mats at 7 d and 14 d. This mineralized composite nanofibrous mats may have a great potential for bone tissue engineering.展开更多
基金Supported by the National Natural Science Foundation of China(No.50673032)the Graduate Student Innovation Foundation of Jilin University,China(No.20111035)
文摘To investigate the contribution of fibre arrangement to guiding the aligned growth of corneal stroma cells,aligned and randomly oriented fibrous scaffolds of gelatin and poly-L-lactic acid（PLLA） were fabricated by electrospinning.A comparative study of two different systems with corneal stroma cells on randomly organized and aligned fibres were conducted.The efficiency of the scaffolds for inducing the aligned growth of cells was assessed by morphological observation and 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl-tetrazolium bromide（MTT） assay.Results show that the cells cultured on both randomly oriented and aligned scaffolds maintained normal morphology and well spreading as well as long term proliferation.Importantly,corneal stroma cells grew high orderly on the aligned scaffold,while the cells grew disordered on the randomly oriented scaffold.Moreover,the cells exhibited higher viability in aligned scaffold than that in randomly oriented scaffold.These results indcate that electrospinng to prepare aligned fibrous scaffolds has provided an effective approach to the aligned growth of corneal stroma cells in vitro.Our findings that fiber arrangement plays a crucial role in guiding the aligned growth of cells may be helpful to the development of better biomaterials for tissue engineered cornea.
基金"111 Project"Biomedical Textile Materials Science and Technology,China(No.B07024)National Natural Science Foundations of China(No.31070871,No.31271035)+2 种基金National Medical Research Council,China(No.NMRC/1151/2008)Technologies Bureau of Jiaxing City,China(No.MTC2012-006,No.2011A Y1026)Science and Technology Agency of Zhejiang Province,China(No.2012R10012-09)
文摘Composite nanofibrous mats consisting of poly( L-lactideco-ε-caprolactone)( PLCL) and collagen type I( COL) were fabricated by electrospinning,and ten times simulated body fluid(10SBF) were employed to mineralize nanofibrous mats. Ballshaped hydroxyapatite( HA) was deposited on the surface of nanofibrous mats in 1. 5 h at room temperature. Human fetal osteoblasts( hFob) were seeded to investigate their proliferation and differentiation on mineralized composite nanofibrous mats. The results showed that hFob grew well on mineralized composite nanofibrous mats and alkaline phosphatase( ALP) activity of hFob on mineralized composite nanofibrous mats at 14 d was much higher than that on untreated nanofibrous mats. Moreover,the expression of osteocalcin of cells on mineralized composite nanofibrous mats was also much higher than those on untreated nanofibrous mats at 7 d and 14 d. This mineralized composite nanofibrous mats may have a great potential for bone tissue engineering.
