Acute and infected wounds resulting from accidents,battlefield trauma,or surgical interventions have become a global healthcare burden due to the complex bacterial infection environment.However,conventional gauze dres...Acute and infected wounds resulting from accidents,battlefield trauma,or surgical interventions have become a global healthcare burden due to the complex bacterial infection environment.However,conventional gauze dressings present insufficient contact with irregular wounds and lack antibacterial activity against multi-drug-resistant bacteria.In this study,we develop in situ nanofibrous dressings tailored tofit wounds of various shapes and sizes while providing nanoscale comfort and excellent antibacterial properties.Our approach involves the fabrication of these dressings using a handheld electrospinning device that allows for the direct depo-sition of nanofiber dressings onto specific irregular wound sites,resulting in perfect conformal wound closure without any mismatch in 2 min.The nanofibrous dressings are loaded with multi-armed antibiotics that exhibit outstanding antibacterial activ-ity against Staphylococcus aureus(S.aureus)and methicillin-resistant S.aureus.Compared to conventional vancomycin,this in situ nanofibrous dressing shows great antibacterial performance against up to 98%of multi-drug-resistant bacteria.In vitro and in vivo experiments demonstrate the ability of in situ nanofibrous dressings to prevent multi-drug-resistant bacterial infection,greatly alleviate inflammation,and promote wound healing.Ourfindings highlight the potential of these personalized nanofibrous dressings for clinical applications,including emergency,accident,and surgical healthcare treatment.展开更多
基金National Key R&D Program of China,Grant/Award Number:2022YFB3804700Guangdong Innovative and Entrepreneurial Research Team Program,Grant/Award Number:2019ZT08Y191+5 种基金Shenzhen Science and Technology Program,Grant/Award Numbers:KQTD20190929172743294,JCYJ20200109141231365National Key Research and Development Program of China,Grant/Award Number:2022YFB3804700Guangdong Major Talent Introduction Project,Grant/Award Number:2019CX01Y196Beijing Institute of Genomics,Chinese Academy of Sciences,Grant/Award Number:QYZDJ-SSW-SLH039Shenzhen Key Laboratory of Smart Healthcare Engineering,Grant/Award Number:ZDSYS20200811144003009National Natural Science Foundation of China,Grant/Award Numbers:21535001,21761142006,22234004,52203243,81730051。
文摘Acute and infected wounds resulting from accidents,battlefield trauma,or surgical interventions have become a global healthcare burden due to the complex bacterial infection environment.However,conventional gauze dressings present insufficient contact with irregular wounds and lack antibacterial activity against multi-drug-resistant bacteria.In this study,we develop in situ nanofibrous dressings tailored tofit wounds of various shapes and sizes while providing nanoscale comfort and excellent antibacterial properties.Our approach involves the fabrication of these dressings using a handheld electrospinning device that allows for the direct depo-sition of nanofiber dressings onto specific irregular wound sites,resulting in perfect conformal wound closure without any mismatch in 2 min.The nanofibrous dressings are loaded with multi-armed antibiotics that exhibit outstanding antibacterial activ-ity against Staphylococcus aureus(S.aureus)and methicillin-resistant S.aureus.Compared to conventional vancomycin,this in situ nanofibrous dressing shows great antibacterial performance against up to 98%of multi-drug-resistant bacteria.In vitro and in vivo experiments demonstrate the ability of in situ nanofibrous dressings to prevent multi-drug-resistant bacterial infection,greatly alleviate inflammation,and promote wound healing.Ourfindings highlight the potential of these personalized nanofibrous dressings for clinical applications,including emergency,accident,and surgical healthcare treatment.
