Electroactive bacteria(EAB)can transfer electrons to exocellular solid acceptors and have widespread applications in the fields of pollutant degradation,biosynthesis,and hydrogen generation.Traditional EAB screening m...Electroactive bacteria(EAB)can transfer electrons to exocellular solid acceptors and have widespread applications in the fields of pollutant degradation,biosynthesis,and hydrogen generation.Traditional EAB screening methods can precisely measure the bacterial extracellular electron transfer ability,but they suffer from time-consuming and labor-intensive procedures.The chemiluminescence technique is effective for the rapid detection of hemoglobin but has not been applied for EAB screening.Herein,we utilized a chemiluminescent assay to identify EAB through a multiheme c-type cytochrome(c-Cyt)triggered chemiluminescent reaction.The multiheme c-Cyts-triggered chemiluminescence was conveniently determined by taking photographs with commercial smartphones and cloud-based computing.The acquired image signal shows a significant and linear relationship with the bacterial concentration.Measurement results of the chemiluminescence intensity triggered by different bacterial species with the proposed method are strongly associated with those of the microplate reading method(Pearson’s r of 0.9795;P<0.01)and dissimilatory Fe(Ⅲ)reduction method(Pearson’s r of 0.9628;P<0.01).Furthermore,this cloud-based and smartphone-assisted chemiluminescent assay is instrument-free and easy to operate,and results can be obtained within 2 min.The findings demonstrate that the proposed method can effectively detect EAB.Therefore,it will be an alternative approach for EAB screening and can have promising applications in microbiology,environmental science,and bioenergy.展开更多
The consumption of non-renewable fossil fuels has directly contributed to a dramatic rise in global carbon dioxide(CO_(2))emissions,posing an ongoing threat to the ecological security of the Earth.Microbial electrosyn...The consumption of non-renewable fossil fuels has directly contributed to a dramatic rise in global carbon dioxide(CO_(2))emissions,posing an ongoing threat to the ecological security of the Earth.Microbial electrosynthesis(MES)is an innovative energy regeneration strategy that offers a gentle and efficient approach to converting CO_(2) into high-value products.The cathode chamber is a vital component of an MES system and its internal factors play crucial roles in improving the performance of the MES system.Therefore,this review aimed to provide a detailed analysis of the key factors related to the cathode chamber in the MES system.The topics covered include inward extracellular electron transfer pathways,cathode materials,applied cathode potentials,catholyte pH,and reactor configuration.In addition,this review analyzes and discusses the challenges and promising avenues for improving the conversion of CO_(2) into high-value products via MES.展开更多
It has been more than one century since the activated sludge process was invented.Despite its proven stability and reliability,the energy(especially the electrical energy)use in wastewater treatment should evolve to m...It has been more than one century since the activated sludge process was invented.Despite its proven stability and reliability,the energy(especially the electrical energy)use in wastewater treatment should evolve to meet the increasingly urgent demand of energy efficiency.This paper discusses how the energy utilized in conventional biological wastewater treatment can be altered by switching the indirect energy input to a direct electricity injection,which is achieved by the elecfrode integration providing extra thermodynamic driving force to biodegradation.By using electrodes instead of oxygen as terminal electron acceptors,the electrical energy can be utilized more efficiently,and the key of direct use of electrical energy in biodegradation is the development of highly active electroactive biofilm and the increase of electron transfer between microbes and the electrode.Furthermore,the synergy of different microbial electrochemical units has additional benefit in energy and resource recovery,making wastewater treatment more sustainable.展开更多
It has been recently suggested that Alcaligenes use a previously unknown pathway to convert ammonium into dinitrogen gas(Dirammox)via hydroxylamine(NH2OH).This fact alone already implies a significant decrease in the ...It has been recently suggested that Alcaligenes use a previously unknown pathway to convert ammonium into dinitrogen gas(Dirammox)via hydroxylamine(NH2OH).This fact alone already implies a significant decrease in the aeration requirements for the process,but the process would still be dependent on external aeration.This work studied the potential use of a polarised electrode as an electron acceptor for ammonium oxidation using the recently described Alcaligenes strain HO-1 as a model heterotrophic nitrifier.Results indicated that Alcaligenes strain HO-1 requires aeration for metabolism,a requirement that cannot be replaced for a polarised electrode alone.However,concomitant elimination of succinate and ammonium was observed when operating a previously grown Alcaligenes strain HO-1 culture in the presence of a polarised electrode and without aeration.The usage of a polarised electrode together with aeration did not increase the succinate nor the nitrogen removal rates observed with aeration alone.However,current density generation was observed along a feeding batch test representing an electron share of 3%of the ammonium removed in the presence of aeration and 16%without aeration.Additional tests suggested that hydroxylamine oxidation to dinitrogen gas could have a relevant role in the electron discharge onto the anode.Therefore,the presence of a polarised electrode supported the metabolic functions of Alcaligenes strain HO-1 on the simultaneous oxidation of succinate and ammonium.展开更多
基金supported by the Guangdong Basic and Applied Basic Research Foundation(Grant No.2021A1515010173)the One-Hundred Young Talents of the Guangdong University of Technology(Grant No.1143–220413696)。
文摘Electroactive bacteria(EAB)can transfer electrons to exocellular solid acceptors and have widespread applications in the fields of pollutant degradation,biosynthesis,and hydrogen generation.Traditional EAB screening methods can precisely measure the bacterial extracellular electron transfer ability,but they suffer from time-consuming and labor-intensive procedures.The chemiluminescence technique is effective for the rapid detection of hemoglobin but has not been applied for EAB screening.Herein,we utilized a chemiluminescent assay to identify EAB through a multiheme c-type cytochrome(c-Cyt)triggered chemiluminescent reaction.The multiheme c-Cyts-triggered chemiluminescence was conveniently determined by taking photographs with commercial smartphones and cloud-based computing.The acquired image signal shows a significant and linear relationship with the bacterial concentration.Measurement results of the chemiluminescence intensity triggered by different bacterial species with the proposed method are strongly associated with those of the microplate reading method(Pearson’s r of 0.9795;P<0.01)and dissimilatory Fe(Ⅲ)reduction method(Pearson’s r of 0.9628;P<0.01).Furthermore,this cloud-based and smartphone-assisted chemiluminescent assay is instrument-free and easy to operate,and results can be obtained within 2 min.The findings demonstrate that the proposed method can effectively detect EAB.Therefore,it will be an alternative approach for EAB screening and can have promising applications in microbiology,environmental science,and bioenergy.
基金supported by grants from National Natural Science Foundation of China (32070097 and 91951202)National Key Research and Development Program of China (2019YFA0904800).
文摘The consumption of non-renewable fossil fuels has directly contributed to a dramatic rise in global carbon dioxide(CO_(2))emissions,posing an ongoing threat to the ecological security of the Earth.Microbial electrosynthesis(MES)is an innovative energy regeneration strategy that offers a gentle and efficient approach to converting CO_(2) into high-value products.The cathode chamber is a vital component of an MES system and its internal factors play crucial roles in improving the performance of the MES system.Therefore,this review aimed to provide a detailed analysis of the key factors related to the cathode chamber in the MES system.The topics covered include inward extracellular electron transfer pathways,cathode materials,applied cathode potentials,catholyte pH,and reactor configuration.In addition,this review analyzes and discusses the challenges and promising avenues for improving the conversion of CO_(2) into high-value products via MES.
基金This work was financially supported by National Natural Science Foundation of China(No.51922051)the Fund for Distinguished Young Scholars of Tianjin(20JCJQJC00040).
文摘It has been more than one century since the activated sludge process was invented.Despite its proven stability and reliability,the energy(especially the electrical energy)use in wastewater treatment should evolve to meet the increasingly urgent demand of energy efficiency.This paper discusses how the energy utilized in conventional biological wastewater treatment can be altered by switching the indirect energy input to a direct electricity injection,which is achieved by the elecfrode integration providing extra thermodynamic driving force to biodegradation.By using electrodes instead of oxygen as terminal electron acceptors,the electrical energy can be utilized more efficiently,and the key of direct use of electrical energy in biodegradation is the development of highly active electroactive biofilm and the increase of electron transfer between microbes and the electrode.Furthermore,the synergy of different microbial electrochemical units has additional benefit in energy and resource recovery,making wastewater treatment more sustainable.
基金funded through the European Union's Horizon 2020 project ELECTRA[no.826244]National Nature Science Foundation of China(grant no.31861133002)+1 种基金S.P is a Serra Húnter Fellow(UdGAG-575)acknowledges the funding from the ICREA Academia award.LEQUiA[2021-SGR-01352]and Ecoaqua[2021-SGR-01142]have been recognized as consolidated research groups by the Catalan Government.
文摘It has been recently suggested that Alcaligenes use a previously unknown pathway to convert ammonium into dinitrogen gas(Dirammox)via hydroxylamine(NH2OH).This fact alone already implies a significant decrease in the aeration requirements for the process,but the process would still be dependent on external aeration.This work studied the potential use of a polarised electrode as an electron acceptor for ammonium oxidation using the recently described Alcaligenes strain HO-1 as a model heterotrophic nitrifier.Results indicated that Alcaligenes strain HO-1 requires aeration for metabolism,a requirement that cannot be replaced for a polarised electrode alone.However,concomitant elimination of succinate and ammonium was observed when operating a previously grown Alcaligenes strain HO-1 culture in the presence of a polarised electrode and without aeration.The usage of a polarised electrode together with aeration did not increase the succinate nor the nitrogen removal rates observed with aeration alone.However,current density generation was observed along a feeding batch test representing an electron share of 3%of the ammonium removed in the presence of aeration and 16%without aeration.Additional tests suggested that hydroxylamine oxidation to dinitrogen gas could have a relevant role in the electron discharge onto the anode.Therefore,the presence of a polarised electrode supported the metabolic functions of Alcaligenes strain HO-1 on the simultaneous oxidation of succinate and ammonium.
