The DNA vaccine pCIHA5 encoding hemagglutinin can protect SPF chicken against lethal H5N1 avian influenza virus challenge. The more characters about its protection efficacity were studied. The protective rates in 10...The DNA vaccine pCIHA5 encoding hemagglutinin can protect SPF chicken against lethal H5N1 avian influenza virus challenge. The more characters about its protection efficacity were studied. The protective rates in 10, 40, 70, 100 and 150 μg groups immunized with pCIHA5 were 12.5 (1/8), 58.3 (7/12), 72.7 (8/11), 50.0 (6/12) and 66.7% (8/12), respectively. The protective rates in 5, 20, 35 and 50 μg groups were 145.5 (5/11), 58.3 (7/12), 58.3 (7/12) and 91.7% (11/12), respectively. The 70, 100 and 5 μg groups have virus shedding of 1/8, 2/6 and 1/5. Though the inactived oil-emulsion vaccine has high HI antibody titers and 100% protective rate, the AGP antibody could be detected after vaccination. Results show that the pCIHA5 is fit to boost by intramuscular injection. This would be useful to the study on gene engineering vaccine of avian influenza virus.展开更多
AIM To evaluate portal vein(PV) stenosis and stent patency after hepatobiliary and pancreatic surgery, using abdominal computed tomography(CT).METHODS Percutaneous portal venous stenting was attempted in 22 patients w...AIM To evaluate portal vein(PV) stenosis and stent patency after hepatobiliary and pancreatic surgery, using abdominal computed tomography(CT).METHODS Percutaneous portal venous stenting was attempted in 22 patients with significant PV stenosis(> 50%)-after hepatobiliary or pancreatic surgery- diagnosed by abdominal CT. Stents were placed in various stenotic lesions after percutaneous transhepatic portography. Pressure gradient across the stenotic segment was measured in 14 patients. Stents were placed when the pressure gradient across the stenotic segment was > 5 mm Hg or PV stenosis was > 50%, as observed on transhepatic portography. Patients underwent followup abdominal CT and technical and clinical success, complications, and stent patency were evaluated.RESULTS Stent placement was successful in 21 patients(technical success rate: 95.5%). Stents were positioned through the main PV and superior mesenteric vein(n = 13), main PV(n = 2), right and main PV(n = 1), left and main PV(n = 4), or main PV and splenic vein(n = 1). Patients showed no complications after stent placement. The time between procedure and final follow-up CT was 41-761 d(mean: 374.5 d). Twenty stents remained patent during the entire follow-up. Stent obstruction-caused by invasion of the PV stent by a recurrent tumor- was observed in 1 patient in a follow-up CT performed after 155 d after the procedure. The cumulative stent patency rate was 95.7%. Small in-stent low-density areas were found in 11(55%) patients; however, during successive follow-up CT, the extent of these areas had decreased.CONCLUSION Percutaneous transhepatic stent placement can be safe and effective in cases of PV stenosis after hepatobiliary and pancreatic surgery. Stents show excellent patency in follow-up abdominal CT, despite development of small in-stent low-density areas.展开更多
Background:High-definition transcranial direct current stimulation(HD-tDCS)and repetitive transcranial magnetic stimulation(rTMS)demonstrate significant potential for improving depressive symptoms and cognitive functi...Background:High-definition transcranial direct current stimulation(HD-tDCS)and repetitive transcranial magnetic stimulation(rTMS)demonstrate significant potential for improving depressive symptoms and cognitive function;however,their effectiveness varies greatly among individuals.Functional near-infrared spectroscopy enables real-time monitoring of brain function during cognitive tasks in patients with psychiatric disorders.Methods:A 4-week longitudinal study was conducted involving 61 patients with depression and 26 healthy controls.Patients were randomly assigned to HD-tDCS,rTMS,and antidepressant(AD)groups.Changes in depressive symptoms,adverse event rates,and prefrontal cortical oxyhemoglobin concentrations were assessed.Result:At week 4,remission rates were 62.5%(15),61.9%(13),and 62.5%(10)in the HD-tDCS,rTMS,and AD groups,respectively(x^(2)=0.002,p=1.000).Response rates were 66.7%(16),71.4%(15),and 68.8%(11),respectively,with no significant difference between groups(x^(2)=0.12,p=0.941).All groups demonstrated significant improvement in depressive symptoms and cognitive function.The rTMS group exhibited a significantly greater decrease in Hamilton Depression Scale score compared with the HDtDCS and AD groups.After 2 weeks of treatment,patients exhibited improved depressive symptoms and reduced activation during the verbalfluency task.However,these changes were not significantly correlated(r=-0.159 to 0.240,p=0.121-0.988).Limitations:All patients had concomitant use of ADs,which may impact near-infrared spectroscopy signaling and have an indeterminate effect on cognition.Conclusion:HD-tDCS,rTMS,and ADs were equally effective,safe,and well-tolerated.HD-tDCS and rTMS were more effective for working memory,attention,executive functioning,and mood regulation.展开更多
文摘The DNA vaccine pCIHA5 encoding hemagglutinin can protect SPF chicken against lethal H5N1 avian influenza virus challenge. The more characters about its protection efficacity were studied. The protective rates in 10, 40, 70, 100 and 150 μg groups immunized with pCIHA5 were 12.5 (1/8), 58.3 (7/12), 72.7 (8/11), 50.0 (6/12) and 66.7% (8/12), respectively. The protective rates in 5, 20, 35 and 50 μg groups were 145.5 (5/11), 58.3 (7/12), 58.3 (7/12) and 91.7% (11/12), respectively. The 70, 100 and 5 μg groups have virus shedding of 1/8, 2/6 and 1/5. Though the inactived oil-emulsion vaccine has high HI antibody titers and 100% protective rate, the AGP antibody could be detected after vaccination. Results show that the pCIHA5 is fit to boost by intramuscular injection. This would be useful to the study on gene engineering vaccine of avian influenza virus.
文摘AIM To evaluate portal vein(PV) stenosis and stent patency after hepatobiliary and pancreatic surgery, using abdominal computed tomography(CT).METHODS Percutaneous portal venous stenting was attempted in 22 patients with significant PV stenosis(> 50%)-after hepatobiliary or pancreatic surgery- diagnosed by abdominal CT. Stents were placed in various stenotic lesions after percutaneous transhepatic portography. Pressure gradient across the stenotic segment was measured in 14 patients. Stents were placed when the pressure gradient across the stenotic segment was > 5 mm Hg or PV stenosis was > 50%, as observed on transhepatic portography. Patients underwent followup abdominal CT and technical and clinical success, complications, and stent patency were evaluated.RESULTS Stent placement was successful in 21 patients(technical success rate: 95.5%). Stents were positioned through the main PV and superior mesenteric vein(n = 13), main PV(n = 2), right and main PV(n = 1), left and main PV(n = 4), or main PV and splenic vein(n = 1). Patients showed no complications after stent placement. The time between procedure and final follow-up CT was 41-761 d(mean: 374.5 d). Twenty stents remained patent during the entire follow-up. Stent obstruction-caused by invasion of the PV stent by a recurrent tumor- was observed in 1 patient in a follow-up CT performed after 155 d after the procedure. The cumulative stent patency rate was 95.7%. Small in-stent low-density areas were found in 11(55%) patients; however, during successive follow-up CT, the extent of these areas had decreased.CONCLUSION Percutaneous transhepatic stent placement can be safe and effective in cases of PV stenosis after hepatobiliary and pancreatic surgery. Stents show excellent patency in follow-up abdominal CT, despite development of small in-stent low-density areas.
基金funded by the Research and Innovation Support Project for Postgraduates at Xinxiang Medical University(YJSCX202203Z,YJSCX202253Y,YJSCX202293Y,YJSCX2022115Y)the Second Affiliated Hospital of Xinxiang Medical University opened the project of psychiatry(XYEFYJSSJ-2023-07)+1 种基金Henan Provincial Key Laboratory of Nerve Repair(HNSJXF-2021-003)the Project of Higher Education Research of Henan Province(2021SXHLX072).
文摘Background:High-definition transcranial direct current stimulation(HD-tDCS)and repetitive transcranial magnetic stimulation(rTMS)demonstrate significant potential for improving depressive symptoms and cognitive function;however,their effectiveness varies greatly among individuals.Functional near-infrared spectroscopy enables real-time monitoring of brain function during cognitive tasks in patients with psychiatric disorders.Methods:A 4-week longitudinal study was conducted involving 61 patients with depression and 26 healthy controls.Patients were randomly assigned to HD-tDCS,rTMS,and antidepressant(AD)groups.Changes in depressive symptoms,adverse event rates,and prefrontal cortical oxyhemoglobin concentrations were assessed.Result:At week 4,remission rates were 62.5%(15),61.9%(13),and 62.5%(10)in the HD-tDCS,rTMS,and AD groups,respectively(x^(2)=0.002,p=1.000).Response rates were 66.7%(16),71.4%(15),and 68.8%(11),respectively,with no significant difference between groups(x^(2)=0.12,p=0.941).All groups demonstrated significant improvement in depressive symptoms and cognitive function.The rTMS group exhibited a significantly greater decrease in Hamilton Depression Scale score compared with the HDtDCS and AD groups.After 2 weeks of treatment,patients exhibited improved depressive symptoms and reduced activation during the verbalfluency task.However,these changes were not significantly correlated(r=-0.159 to 0.240,p=0.121-0.988).Limitations:All patients had concomitant use of ADs,which may impact near-infrared spectroscopy signaling and have an indeterminate effect on cognition.Conclusion:HD-tDCS,rTMS,and ADs were equally effective,safe,and well-tolerated.HD-tDCS and rTMS were more effective for working memory,attention,executive functioning,and mood regulation.
