Aims:To compare the efficacy and safety of vonoprazan-based bismuth-containing quadruple therapy(VBCQ)versus proton pump inhibitor(PPI)-based bismuth-containing quadruple Therapy(PBCQ)for Helicobacter pylorieradicatio...Aims:To compare the efficacy and safety of vonoprazan-based bismuth-containing quadruple therapy(VBCQ)versus proton pump inhibitor(PPI)-based bismuth-containing quadruple Therapy(PBCQ)for Helicobacter pylorieradication.Methods:We performed a systematic search in PubMed,Embase,Web of Science,Cochrane Library,CNKI,Wanfang databases for relevant randomized controlled trials up to March 2024.Meta-analysis by RevMan 5.4 software.Results:Ten randomized controlled trials were evaluated in this meta-analysis.In intention-to-treat(ITT)analysis and per-protocol(PP)analysis,the eradication rate of VBCQ was signifi cantly higher than PBCQ[ITT overall eradication rate:86.7%vs 82.8%,RR:1.05,95%CI(1.05,1.09),P<0.05;PP overall eradication rate:92.0%vs 88.0%;RR=1.05,95%CI(1.05,1.08),P<0.05].The incidence of adverse events in VBCQ was higher than PBCQ(36.7%vs 36.5%),there was no signifi cant diff erence(P>0.05).Conclusions:VBCQ has a higher eradication rate and comparable safety.展开更多
Molecular cloning remains a cornerstone technique in genetic engineering and synthetic biology.In this study,we conducted a systematic comparative analysis between the classical cloning method and the Golden Gate asse...Molecular cloning remains a cornerstone technique in genetic engineering and synthetic biology.In this study,we conducted a systematic comparative analysis between the classical cloning method and the Golden Gate assembly technique,utilizing Escherichia coli as the model organism.Through polymerase chain reaction(PCR)amplification,restriction enzyme digestion,ligation,transformation,and Sanger sequencing,we assessed the operational efficiency and cloning fidelity of both strategies.Our results demonstrated that Golden Gate assembly,leveraging type IIS restriction enzymes and simultaneous ligation,significantly enhanced cloning efficiency and precision,particularly for seamless multi-fragment assembly.In contrast,the classical cloning approach maintained certain advantages in simplicity and robustness for specific experimental conditions.Challenges encountered during transformation and sequencing highlighted the critical impact of technical accuracy on experimental outcomes.This study underscores the importance of selecting appropriate cloning methodologies tailored to experimental objectives and laboratory capabilities,providing a foundation for optimized molecular cloning workflows in future synthetic biology and biotechnology applications.展开更多
AIM: To investigate the efficacy of tandospirone in patients with irritable bowel syndrome-diarrhea (IBS-D) and anxiety in a prospective, randomized, controlled study.
Based on the Data Envelopment Analysis method,and by using CCR and BCC model,Super Efficiency model and Malmquist model guided by input efficiency,the input-output efficiency of elements of urban construction land in ...Based on the Data Envelopment Analysis method,and by using CCR and BCC model,Super Efficiency model and Malmquist model guided by input efficiency,the input-output efficiency of elements of urban construction land in different jurisdictions of Beijing from 2005 to 2015 was studied.The results showed that there were obvious differences between input-output efficiency of elements of urban construction land in different jurisdictions of Beijing,among which the efficiency of the core area of capital,Yanqing District,Fangshan District and Huairou District was relatively high,while the efficiency of Daxing District,Fengtai District and Miyun District was relatively low.There was no obvious correlation between efficiency differentiation and location factors,which is mainly caused by whether the land use in each jurisdiction has scale effect,whether the technology is improved,whether the input is redundant and whether the output is insufficient.For the jurisdiction of inefficient land use,we should strengthen the consciousness of intensive land use,improve the technical level,appropriately reduce the redundancy of input elements,and pay attention to the output of social and ecological benefits.展开更多
This study seeks to evaluate the comparative productivity of 32 listed tourism companies which are the main suppliers of China tourism, using the popular methodology known as the data envelopment analysis(DEA). This s...This study seeks to evaluate the comparative productivity of 32 listed tourism companies which are the main suppliers of China tourism, using the popular methodology known as the data envelopment analysis(DEA). This study analyzes the productivity of listed tourism companies from business and region aspects based on the calculation of Malmquist index. The results show that(1) the overall productivity is non-effi cient(0.954);(2) the productivity of accommodation and catering is biggest, which shows the tourism develops quickly with supports from technology;(3) the productivity in western China is highest, where the economy and tourism attraction are better than other regions; and(4) the effi ciency differences among the listed tourism companies are not signifi cant, and they attribute to the scale effi-ciency, that is the input of the fi nance, resource, talents and policy.展开更多
<strong>Objective:</strong> The objective is to explore the long-term efficacy of total enteral nutrition in the treatment of abdominal abscess in Crohn’s disease. <strong>Methods: </strong>Pa...<strong>Objective:</strong> The objective is to explore the long-term efficacy of total enteral nutrition in the treatment of abdominal abscess in Crohn’s disease. <strong>Methods: </strong>Patients treated with EEN in our hospital and whose abdominal abscess disappeared after 12 weeks of treatment were included, and the data of abscess recurrence and surgical treatment during follow-up were included. <strong>Results:</strong> A total of 44 consecutive cases meeting the criteria were included. The 1-year and 2-year recurrence rates were 22.5% and 39.9% respectively. Among the patients with recurrence, 10 patients chose EEN treatment again, and 5 patients received direct surgical treatment. Of the patients who chose to undergo EEN treatment again, 8 still eventually required surgery. The 1-year operative rate was 16.9%, and the 2-year operative rate was 35.6%. The median operative time was 33.3 (95% CI: 21.3, 45.4) months. <strong>Conclusions: </strong>The 2-year cumulative incidence of recurrence of abdominal abscess in CD patients whose abdominal abscess disappeared after EEN was 39.9%, and the 2-year cumulative surgical rate was 35.6%. The operative stomy rate decreased after EEN, and the primary anastomosis rate increased significantly.展开更多
Objective: to determine the efficacy, intraocular pressure, and visual field defects of eyes with primary open-angle glaucoma treated with travoprost ophthalmic solution. Methods: a total of 120 primary open-angle gla...Objective: to determine the efficacy, intraocular pressure, and visual field defects of eyes with primary open-angle glaucoma treated with travoprost ophthalmic solution. Methods: a total of 120 primary open-angle glaucoma patients who volunteered to participate were recruited as test subjects and divided into a control group (n 60, pilocarpine eye drop therapy) versus an observation group (n 60, travoprost eye drop therapy) according to the randomization principle, and the time of the patients admission to the ophthalmology department was in the range of February 2020 to August 2021. The clinical effects after treatment were compared between different groups;The IOP and visual field defects were analyzed before and after treatment in different groups. Results: the overall response rate was 66.67% in the control group while it was 90.00% in the observation group, which was obviously an excellent treatment modality( χ 28.298,P0.004);There was no difference in the IOP performance of the different groups before treatment (P > 0.05), and the IOP improvement after treatment showed a trend toward reduction, and the reduction was more pronounced in the observation group (P 0.05);There was no significant difference in the incidence of conjunctival hyperemia between the two groups (P 1.000), and periocular hyperpigmentation was observed in the observation group compared with the control group( χ 2 4.386, P 0.036) and thicker and longer eyelashes( χ 2 9.784, P 0.002). Conclusions: travoprost ophthalmic solution, when used in the treatment of patients with primary open angle glaucoma, although it can lead to the thickening of the patients eyelashes growth and periocular pigmentation, it can significantly improve the patients clinical efficacy, has an indispensable role in reducing intraocular pressure and protecting the patients visual field and vision, and has high clinical application and promotion value.展开更多
In this paper, we study the efficiency issue of inexact Newton-type methods for smooth unconstrained optimization problems under standard assumptions from theoretical point of view by discussing a concrete Newton-PCG ...In this paper, we study the efficiency issue of inexact Newton-type methods for smooth unconstrained optimization problems under standard assumptions from theoretical point of view by discussing a concrete Newton-PCG algorithm. In order to compare the algorithm with Newton's method, a ratio between the measures of their approximate efficiencies is investigated. Under mild conditions, it is shown that first, this ratio is larger than 1, which implies that the Newton-PCG algorithm is more efficient than Newton's method, and second, this ratio increases when the dimension n of the problem increases and tends to infinity at least at a rate In n/In 2 when n→∞, which implies that in theory the Newton-PCG algorithm is much more efficient for middle- and large-scale problems. These theoretical results are also supported by our preliminary numerical experiments.展开更多
Extensive urban land expansion and heavy industrialization have increased energy consumption and caused environmental problems, both of which present serious threats to humans. Consequently, improved land use efficien...Extensive urban land expansion and heavy industrialization have increased energy consumption and caused environmental problems, both of which present serious threats to humans. Consequently, improved land use efficiency and realization of green development are imperative. Based on a detailed analysis of spatial- temporal evolution of urban land use efficiency, this paper analyzes the synergistic effect of industrial structure and city size, as well as the effect of environmental quality, by using panel data from 283 cities at or above prefecture- level in China from 2003 to 2012. It was concluded that 1） environmental quality has an obvious ＂crowding out effect＂ on urban land use efficiency and 2） urban land use efficiency shows a significant spatial auto-correlation. The effect of industrial structure is dependent on popula- tion size of the city. It has been found that a threshold population size of more than 108.45 （10,000 persons） is needed for an optimized benefit from industrial linkages. The urban population size presents an inverted-U shape against the urban land use efficiency, and the marginal benefit of urban size increases when the industrial structure shifts from secondary industry to tertiary industry. Additionally we found that the actual urban size of 98.2% is less than the cities＇ optimal sizes.展开更多
文摘Aims:To compare the efficacy and safety of vonoprazan-based bismuth-containing quadruple therapy(VBCQ)versus proton pump inhibitor(PPI)-based bismuth-containing quadruple Therapy(PBCQ)for Helicobacter pylorieradication.Methods:We performed a systematic search in PubMed,Embase,Web of Science,Cochrane Library,CNKI,Wanfang databases for relevant randomized controlled trials up to March 2024.Meta-analysis by RevMan 5.4 software.Results:Ten randomized controlled trials were evaluated in this meta-analysis.In intention-to-treat(ITT)analysis and per-protocol(PP)analysis,the eradication rate of VBCQ was signifi cantly higher than PBCQ[ITT overall eradication rate:86.7%vs 82.8%,RR:1.05,95%CI(1.05,1.09),P<0.05;PP overall eradication rate:92.0%vs 88.0%;RR=1.05,95%CI(1.05,1.08),P<0.05].The incidence of adverse events in VBCQ was higher than PBCQ(36.7%vs 36.5%),there was no signifi cant diff erence(P>0.05).Conclusions:VBCQ has a higher eradication rate and comparable safety.
文摘Molecular cloning remains a cornerstone technique in genetic engineering and synthetic biology.In this study,we conducted a systematic comparative analysis between the classical cloning method and the Golden Gate assembly technique,utilizing Escherichia coli as the model organism.Through polymerase chain reaction(PCR)amplification,restriction enzyme digestion,ligation,transformation,and Sanger sequencing,we assessed the operational efficiency and cloning fidelity of both strategies.Our results demonstrated that Golden Gate assembly,leveraging type IIS restriction enzymes and simultaneous ligation,significantly enhanced cloning efficiency and precision,particularly for seamless multi-fragment assembly.In contrast,the classical cloning approach maintained certain advantages in simplicity and robustness for specific experimental conditions.Challenges encountered during transformation and sequencing highlighted the critical impact of technical accuracy on experimental outcomes.This study underscores the importance of selecting appropriate cloning methodologies tailored to experimental objectives and laboratory capabilities,providing a foundation for optimized molecular cloning workflows in future synthetic biology and biotechnology applications.
文摘AIM: To investigate the efficacy of tandospirone in patients with irritable bowel syndrome-diarrhea (IBS-D) and anxiety in a prospective, randomized, controlled study.
文摘Based on the Data Envelopment Analysis method,and by using CCR and BCC model,Super Efficiency model and Malmquist model guided by input efficiency,the input-output efficiency of elements of urban construction land in different jurisdictions of Beijing from 2005 to 2015 was studied.The results showed that there were obvious differences between input-output efficiency of elements of urban construction land in different jurisdictions of Beijing,among which the efficiency of the core area of capital,Yanqing District,Fangshan District and Huairou District was relatively high,while the efficiency of Daxing District,Fengtai District and Miyun District was relatively low.There was no obvious correlation between efficiency differentiation and location factors,which is mainly caused by whether the land use in each jurisdiction has scale effect,whether the technology is improved,whether the input is redundant and whether the output is insufficient.For the jurisdiction of inefficient land use,we should strengthen the consciousness of intensive land use,improve the technical level,appropriately reduce the redundancy of input elements,and pay attention to the output of social and ecological benefits.
基金supported by the project of Shaanxi Normal University(Grant No.999521)Xianyang Normal University(Grant Nos.11XSYK316,201002001)
文摘This study seeks to evaluate the comparative productivity of 32 listed tourism companies which are the main suppliers of China tourism, using the popular methodology known as the data envelopment analysis(DEA). This study analyzes the productivity of listed tourism companies from business and region aspects based on the calculation of Malmquist index. The results show that(1) the overall productivity is non-effi cient(0.954);(2) the productivity of accommodation and catering is biggest, which shows the tourism develops quickly with supports from technology;(3) the productivity in western China is highest, where the economy and tourism attraction are better than other regions; and(4) the effi ciency differences among the listed tourism companies are not signifi cant, and they attribute to the scale effi-ciency, that is the input of the fi nance, resource, talents and policy.
文摘<strong>Objective:</strong> The objective is to explore the long-term efficacy of total enteral nutrition in the treatment of abdominal abscess in Crohn’s disease. <strong>Methods: </strong>Patients treated with EEN in our hospital and whose abdominal abscess disappeared after 12 weeks of treatment were included, and the data of abscess recurrence and surgical treatment during follow-up were included. <strong>Results:</strong> A total of 44 consecutive cases meeting the criteria were included. The 1-year and 2-year recurrence rates were 22.5% and 39.9% respectively. Among the patients with recurrence, 10 patients chose EEN treatment again, and 5 patients received direct surgical treatment. Of the patients who chose to undergo EEN treatment again, 8 still eventually required surgery. The 1-year operative rate was 16.9%, and the 2-year operative rate was 35.6%. The median operative time was 33.3 (95% CI: 21.3, 45.4) months. <strong>Conclusions: </strong>The 2-year cumulative incidence of recurrence of abdominal abscess in CD patients whose abdominal abscess disappeared after EEN was 39.9%, and the 2-year cumulative surgical rate was 35.6%. The operative stomy rate decreased after EEN, and the primary anastomosis rate increased significantly.
文摘Objective: to determine the efficacy, intraocular pressure, and visual field defects of eyes with primary open-angle glaucoma treated with travoprost ophthalmic solution. Methods: a total of 120 primary open-angle glaucoma patients who volunteered to participate were recruited as test subjects and divided into a control group (n 60, pilocarpine eye drop therapy) versus an observation group (n 60, travoprost eye drop therapy) according to the randomization principle, and the time of the patients admission to the ophthalmology department was in the range of February 2020 to August 2021. The clinical effects after treatment were compared between different groups;The IOP and visual field defects were analyzed before and after treatment in different groups. Results: the overall response rate was 66.67% in the control group while it was 90.00% in the observation group, which was obviously an excellent treatment modality( χ 28.298,P0.004);There was no difference in the IOP performance of the different groups before treatment (P > 0.05), and the IOP improvement after treatment showed a trend toward reduction, and the reduction was more pronounced in the observation group (P 0.05);There was no significant difference in the incidence of conjunctival hyperemia between the two groups (P 1.000), and periocular hyperpigmentation was observed in the observation group compared with the control group( χ 2 4.386, P 0.036) and thicker and longer eyelashes( χ 2 9.784, P 0.002). Conclusions: travoprost ophthalmic solution, when used in the treatment of patients with primary open angle glaucoma, although it can lead to the thickening of the patients eyelashes growth and periocular pigmentation, it can significantly improve the patients clinical efficacy, has an indispensable role in reducing intraocular pressure and protecting the patients visual field and vision, and has high clinical application and promotion value.
基金supported by the National Natural Science Foundation of China(Grant No..10371131)the Strategic Research of City University of Hong Kong(Grant No.7001713).
文摘In this paper, we study the efficiency issue of inexact Newton-type methods for smooth unconstrained optimization problems under standard assumptions from theoretical point of view by discussing a concrete Newton-PCG algorithm. In order to compare the algorithm with Newton's method, a ratio between the measures of their approximate efficiencies is investigated. Under mild conditions, it is shown that first, this ratio is larger than 1, which implies that the Newton-PCG algorithm is more efficient than Newton's method, and second, this ratio increases when the dimension n of the problem increases and tends to infinity at least at a rate In n/In 2 when n→∞, which implies that in theory the Newton-PCG algorithm is much more efficient for middle- and large-scale problems. These theoretical results are also supported by our preliminary numerical experiments.
文摘Extensive urban land expansion and heavy industrialization have increased energy consumption and caused environmental problems, both of which present serious threats to humans. Consequently, improved land use efficiency and realization of green development are imperative. Based on a detailed analysis of spatial- temporal evolution of urban land use efficiency, this paper analyzes the synergistic effect of industrial structure and city size, as well as the effect of environmental quality, by using panel data from 283 cities at or above prefecture- level in China from 2003 to 2012. It was concluded that 1） environmental quality has an obvious ＂crowding out effect＂ on urban land use efficiency and 2） urban land use efficiency shows a significant spatial auto-correlation. The effect of industrial structure is dependent on popula- tion size of the city. It has been found that a threshold population size of more than 108.45 （10,000 persons） is needed for an optimized benefit from industrial linkages. The urban population size presents an inverted-U shape against the urban land use efficiency, and the marginal benefit of urban size increases when the industrial structure shifts from secondary industry to tertiary industry. Additionally we found that the actual urban size of 98.2% is less than the cities＇ optimal sizes.
