OBJECTIVE:To investigate the short-term efficacy and safety outcomes following a sequential treatment with clearing heat and eliminating phlegm(CHEP)formula and tonifying Qi and activating blood circulation(TQABC)form...OBJECTIVE:To investigate the short-term efficacy and safety outcomes following a sequential treatment with clearing heat and eliminating phlegm(CHEP)formula and tonifying Qi and activating blood circulation(TQABC)formula in patients with acute ischemic stroke(AIS)within a 72 h time window.METHODS:In this randomized,multicenter,doubleblinded,placebo-controlled trial,500 participants will be randomly assigned in a ratio of 1∶1 to the CHEP+TQABC group or control group.In addition to guidelinebased standard medical care,participants in the treatment group will receive the CHEP formula for the first 5 consecutive days followed by the TQABC formula for another 10 consecutive days,while those in the control group will receive CHEP formula placebo and TQABC formula placebo consecutively.The primary outcome measure will be the comparison of the change in the National Institutes of Health Stroke Scale score from baseline to 15 days after randomization.The secondary outcome measures will include the scores on the modified Rankin Scale,Barthel Index,Patient-Reported Outcomes,TCM symptom pattern(Zheng-hou)evaluation Scale,and the incidence of in-hospital complications.Safety assessment will include the physical examination,laboratory detection,any adverse events or serious adverse events,and the proportion of any complications during hospitalization.DISCUSSION:The results of this study will provide objective and scientific data with which to assess the efficacy and safety of a sequential treatment based on“integrating disease and symptom pattern”for patients with AIS.展开更多
Objective: Observing the expression changes of serum proteome in model rats after intervention of the Granules of Eliminating Phlegm and Removing Blood Stasis (豁痰祛瘀颗粒 also known as GEPRB), screening out and iden...Objective: Observing the expression changes of serum proteome in model rats after intervention of the Granules of Eliminating Phlegm and Removing Blood Stasis (豁痰祛瘀颗粒 also known as GEPRB), screening out and identifying the differentially expressed proteins by mass spectrometry and bioinformatics analysis, discussing the molecular mechanism of control the Diabetes deafness by GEPRB. Methods: By use of proteomics technology, the serum protein serum proteome of the control group, model control group, Duxil and each observation group were observed for 2-DE gel pattern matching, and the difference in the relative content of 2 times was chosen for the differentially expressed proteins. Identification of differentially expressed proteins by MALDI-TOF MS/MS, the authors further analysis the phosphorylation, subcellular localization, interaction, direct regulation, and transmembrane of the differences proteins by the way of bioinformatics analysis. Sixty SPF level SD rats elected in diabetic rats model group (abbreviated as DM group) were be randomly divided into 5 groups based on random number sheet, namely model control group, positive drug control group (Du-ke-xi group) and Mai-tong-fang high, medium and low dose group respectively. In addition, set of normal control group. 10 rats in each group. Results: By Coomassie brilliant blue staining, identified 51 differential protein spots dug from 2-D gel by mass spectrometry, successfully identified 13 non-redundant proteins. Most of the identified proteins were secreted protein and belong to different protein families. There were about 12 proteins have the transmembrane region from the authors’ result, ten of them were plasma membrane proteins. Conclusion: It’s suggesting that 13 differential proteins is most likely the protein response to GEPRB in vivo, these proteins may play key role for the treatment of GEPRB to Diabetes deafness. The two highly differentially expressed proteins Apolipoprotein E (apoE) and C3 may be a potential drug target of GEPRB.展开更多
Objective:To systematically evaluate the efficacy and safety of the method of tonifying qi,resolving phlegm and eliminating stasis in the treatment of acute exacerbation of chronic obstructive pulmonary disease.Method...Objective:To systematically evaluate the efficacy and safety of the method of tonifying qi,resolving phlegm and eliminating stasis in the treatment of acute exacerbation of chronic obstructive pulmonary disease.Methods:Search the China Knowledge Network(CNKI),VIP Chinese Science and Technology Journal Database(VIP),Wanfang Data Knowledge Service Platform,China Biomedical Literature Database(CBM),PubMed,Embase,Cochrane Library,and select the databases that meet the requirements for tonifying qi,resolving phlegm and eliminating stasis.The stasis method in the treatment of AECOPD was included in the standard literature,and RevMan 5.4 software was used for statistical analysis.Results:A total of 10 articles were included,with a total of 854 patients,435 in the treatment group and 419 in the control group.Systematic analysis shows that the method of tonifying qi,resolving phlegm and eliminating stasis combined with conventional Western medicine treatment of AECOPD is significantly different in total clinical effective rate than conventional Western medicine treatment alone[OR=3.98,95%CI(2.23,7.11),P<0.00001];In terms of lung function,FEV1/FVC[MD=6.08,95%CI(5.01,7.15),P<0.00001],FEV1[MD=0.07,95%CI(0.01,0.13),P=0.02],FEV1%[MD=4.56,95%CI(3.09,6.02),P<0.00001]is significantly higher than the control group;arterial oxygen partial pressure(PaO_(2))[MD=18.47,95%CI(16.77,20.16),P<0.00001]Significant improvement compared to the control group;arterial partial pressure of carbon dioxide(PaCO_(2))[MD=-7.48,95%CI(-8.7,-6.26),P<0.00001]was significantly lower than the control group.Conclusion:The current evidence shows that the method of tonifying qi,resolving phlegm and eliminating stasis combined with conventional Western medicine treatment of chronic obstructive pulmonary disease in acute exacerbations can improve clinical efficacy and improve the quality of life of patients.展开更多
Objective:To observe the clinical effect of electroacupuncture on improving polycystic ovary syndrome(PCOS)of kidney deficiency and phlegm dampness type and serum oxidative stress level.Methods:There were 68 patients ...Objective:To observe the clinical effect of electroacupuncture on improving polycystic ovary syndrome(PCOS)of kidney deficiency and phlegm dampness type and serum oxidative stress level.Methods:There were 68 patients with polycystic ovary syndrome were randomly divided into observation group(34 cases)and the control group(34 cases),The observation group was treated by electroacupuncture,while the control group was treated by placebo electroacupuncture.Combined with the evaluation of menstrual cycle,menstrual color,quality,quantity and TCM symptom integral index,the difference of curative effect between the two groups was observed,and the levels of peripheral blood,sex hormones,insulin resistance and oxidative stress were detected before and after treatment for 3 months.Results:The total effective rate in the observation group was better than that in the control group(P<0.01);After intervention,serum LH,FSH,E_(2) and LH/FSH in the observation group were significantly different from those before intervention.The D values of serum LH,FSH,LH/FSH and E_(2) before and after intervention were significantly different between the two groups(P<0.01);Comparing the level of androgen,the serum A4 and T in the observation group were significantly different from those before intervention(P<0.01);After intervention,there was a significant difference in serum A4 and T between the two groups(P<0.01),and there was a significant difference in D value between the two groups before and after intervention;There was a difference in insulin resistance in the observation group before and after intervention(P<0.05),and there was a significant difference in D value between the two groups before and after intervention(P<0.01);After the intervention,the serum SOD,MDA,ROS in the observation group were significantly different from those before the intervention;After intervention,the results of serum SOD and ROS in the observation group were significantly different from those in the control group;There was no significant difference in serum MDA results.There were significant differences in SOD,MDA,ROS between the two groups before and after intervention.Conclusion:Electroacupuncture can improve oxidative stress injury,insulin resistance and hyperandrogenism in PCOS patients.展开更多
Objective:This study mainly explores the clinical effect of dispelling wind,eliminating lung and relieving cough combined with western medicine in the treatment of cough variant asthma.Methods:80 children with cough v...Objective:This study mainly explores the clinical effect of dispelling wind,eliminating lung and relieving cough combined with western medicine in the treatment of cough variant asthma.Methods:80 children with cough variant asthma accepted by our hospital from January 2018 to December 2020 were randomly selected for the study and divided into two groups.One group was the reference group(40 cases)treated with procaterol hydrochloride tablets and montelukast sodium,and the other group was the research group(40 cases)・The method of eliminating wind,eliminating lung and relieving cough was combined with procaterol hydrochloride tablets and montelukast sodium to observe and compare the curative effects of the two groups.Results:There was no significant difference in TCM symptom score and eosinophil(EOS)count between the two groups before treatment(P>0.05);After treatment,the TCM symptom scores of coughs,pharyngeal itching,expectoration,nasal congestion and nasal itching in the research group were lower than those in the reference group,and the EOS count was lower than that in the reference group(P<0.05);The effective rate of research group was higher than that of reference group(P<0.05).Conclusions:For children with cough variant asthma,Qufeng Sufei cough relieving method combined with procaterol hydrochloride and montelukast sodium can improve children's symptoms and reduce eosinophil count.展开更多
Since the water supply crisis in the Taihu Lake on May 29,2007,the control of the Taihu Lake has achieved remarkable results,and the eutrophication has been greatly reduced.Existing problems are as follows:outbreaks o...Since the water supply crisis in the Taihu Lake on May 29,2007,the control of the Taihu Lake has achieved remarkable results,and the eutrophication has been greatly reduced.Existing problems are as follows:outbreaks of blue-green alga still appear seriously every year,and there is no goal to eliminate outbreaks of blue-green alga;the load into the lake greatly exceeds the environmental capacity;reed wetlands are greatly reduced;there is still the possibility of a water supply crisis;the research of eliminating outbreak of blue-green alga is weak.In this paper,the experience of controlling the Taihu Lake is summarized,and the ultimate goals of eliminating eutrophication and outbreaks of blue-green alga and restoring wetlands and biodiversity are proposed.Control measures are proposed,such as deepening the promotion of the lake chief system,establishing the lake chief system in an all-round way,and increasing applied scientific and technological efforts and capital investment.The necessity and possibility of further controlling the Taihu Lake to eliminate outbreaks of blue-green alga should be recognized.Relying on controlling eutrophication alone cannot eliminate outbreaks of blue-green alga.The point that phosphorus control is the key to eliminate outbreaks of blue-green alga is not suitable for the Taihu Lake.The integration ideas of"three types"of technologies should be innovated.The first one is"double reduction and double increase"measures to eliminate eutrophication,of which"double reduction"is to reduce external point sources and non-point sources and internal blue-green alga in sediment;for external sources,the treatment standard of sewage plants should be paid special attention to.The second one is"double algae removal"measures to salvage blue-green alga,suppresses and kills algae.The blue-green alga on the surface,middle and bottom of water should be thoroughly salvaged and eliminated from various water areas.The third one is"double restoration"measures to restore wetlands and biodiversity.The area of wetlands should be restored to the area from the 1960s to the 1970s before outbreaks of blue-green alga,and vegetation coverage reaches 25%-30%.The Taihu Lake is controlled in different waters to ensure water supply safety in water sources.Five suggestions are proposed,such as revising the"Overall Plan for the Comprehensive Treatment of Water Environment in the Taihu Lake Basin"again,incorporating the goal of eliminating outbreaks of blue-green alga in the plan,setting up this research topic,greatly improving sewage treatment standards in local legislation.展开更多
该研究基于药效、血浆药物化学、网络药理学及药代动力学等方法探讨咳宁颗粒止咳祛痰的质量标志物(Q-marker)。通过浓氨水引咳和酚红排泌试验评估咳宁颗粒的止咳、祛痰药效作用。采用超高效液相色谱-四极杆-静电场轨道阱串联质谱(UHPLC-...该研究基于药效、血浆药物化学、网络药理学及药代动力学等方法探讨咳宁颗粒止咳祛痰的质量标志物(Q-marker)。通过浓氨水引咳和酚红排泌试验评估咳宁颗粒的止咳、祛痰药效作用。采用超高效液相色谱-四极杆-静电场轨道阱串联质谱(UHPLC-Q-Exactive Plus Orbitrap HRMS)技术分析鉴定到咳宁颗粒的原型入血成分26个。网络药理学筛选出咳宁颗粒止咳祛痰的11个潜在活性成分,涉及IL6、TLR4、STAT3等40个共同靶点,主要参与PI3K/Akt、HIF-1、EGFR等信号通路。利用药代动力学定量分析了7个原型入血成分。通过有效性、传递性、可测性将壬二酸、咖啡酸、香草醛3个化合物作为咳宁颗粒止咳祛痰的质量标志物。该研究结果对于深入明确咳宁颗粒的药效物质基础、优化质量标准以及推动其临床应用具有重要意义。展开更多
基金Supported by the National Key Research and Development Program of China(Evidence-based Evaluation of TCM Key Syndrome Differentiation and Treatment for Acute Ischemic Stroke,No.2018YFC1705002)
文摘OBJECTIVE:To investigate the short-term efficacy and safety outcomes following a sequential treatment with clearing heat and eliminating phlegm(CHEP)formula and tonifying Qi and activating blood circulation(TQABC)formula in patients with acute ischemic stroke(AIS)within a 72 h time window.METHODS:In this randomized,multicenter,doubleblinded,placebo-controlled trial,500 participants will be randomly assigned in a ratio of 1∶1 to the CHEP+TQABC group or control group.In addition to guidelinebased standard medical care,participants in the treatment group will receive the CHEP formula for the first 5 consecutive days followed by the TQABC formula for another 10 consecutive days,while those in the control group will receive CHEP formula placebo and TQABC formula placebo consecutively.The primary outcome measure will be the comparison of the change in the National Institutes of Health Stroke Scale score from baseline to 15 days after randomization.The secondary outcome measures will include the scores on the modified Rankin Scale,Barthel Index,Patient-Reported Outcomes,TCM symptom pattern(Zheng-hou)evaluation Scale,and the incidence of in-hospital complications.Safety assessment will include the physical examination,laboratory detection,any adverse events or serious adverse events,and the proportion of any complications during hospitalization.DISCUSSION:The results of this study will provide objective and scientific data with which to assess the efficacy and safety of a sequential treatment based on“integrating disease and symptom pattern”for patients with AIS.
文摘Objective: Observing the expression changes of serum proteome in model rats after intervention of the Granules of Eliminating Phlegm and Removing Blood Stasis (豁痰祛瘀颗粒 also known as GEPRB), screening out and identifying the differentially expressed proteins by mass spectrometry and bioinformatics analysis, discussing the molecular mechanism of control the Diabetes deafness by GEPRB. Methods: By use of proteomics technology, the serum protein serum proteome of the control group, model control group, Duxil and each observation group were observed for 2-DE gel pattern matching, and the difference in the relative content of 2 times was chosen for the differentially expressed proteins. Identification of differentially expressed proteins by MALDI-TOF MS/MS, the authors further analysis the phosphorylation, subcellular localization, interaction, direct regulation, and transmembrane of the differences proteins by the way of bioinformatics analysis. Sixty SPF level SD rats elected in diabetic rats model group (abbreviated as DM group) were be randomly divided into 5 groups based on random number sheet, namely model control group, positive drug control group (Du-ke-xi group) and Mai-tong-fang high, medium and low dose group respectively. In addition, set of normal control group. 10 rats in each group. Results: By Coomassie brilliant blue staining, identified 51 differential protein spots dug from 2-D gel by mass spectrometry, successfully identified 13 non-redundant proteins. Most of the identified proteins were secreted protein and belong to different protein families. There were about 12 proteins have the transmembrane region from the authors’ result, ten of them were plasma membrane proteins. Conclusion: It’s suggesting that 13 differential proteins is most likely the protein response to GEPRB in vivo, these proteins may play key role for the treatment of GEPRB to Diabetes deafness. The two highly differentially expressed proteins Apolipoprotein E (apoE) and C3 may be a potential drug target of GEPRB.
基金Joint Fund Project of National Natural Science Foundation of China(No.U20A20398)National Natural Science Foundation of China(No.81974569)the Anhui Provincial Natural Science Foundation of Youth Project(No.1908085QH369)。
文摘Objective:To systematically evaluate the efficacy and safety of the method of tonifying qi,resolving phlegm and eliminating stasis in the treatment of acute exacerbation of chronic obstructive pulmonary disease.Methods:Search the China Knowledge Network(CNKI),VIP Chinese Science and Technology Journal Database(VIP),Wanfang Data Knowledge Service Platform,China Biomedical Literature Database(CBM),PubMed,Embase,Cochrane Library,and select the databases that meet the requirements for tonifying qi,resolving phlegm and eliminating stasis.The stasis method in the treatment of AECOPD was included in the standard literature,and RevMan 5.4 software was used for statistical analysis.Results:A total of 10 articles were included,with a total of 854 patients,435 in the treatment group and 419 in the control group.Systematic analysis shows that the method of tonifying qi,resolving phlegm and eliminating stasis combined with conventional Western medicine treatment of AECOPD is significantly different in total clinical effective rate than conventional Western medicine treatment alone[OR=3.98,95%CI(2.23,7.11),P<0.00001];In terms of lung function,FEV1/FVC[MD=6.08,95%CI(5.01,7.15),P<0.00001],FEV1[MD=0.07,95%CI(0.01,0.13),P=0.02],FEV1%[MD=4.56,95%CI(3.09,6.02),P<0.00001]is significantly higher than the control group;arterial oxygen partial pressure(PaO_(2))[MD=18.47,95%CI(16.77,20.16),P<0.00001]Significant improvement compared to the control group;arterial partial pressure of carbon dioxide(PaCO_(2))[MD=-7.48,95%CI(-8.7,-6.26),P<0.00001]was significantly lower than the control group.Conclusion:The current evidence shows that the method of tonifying qi,resolving phlegm and eliminating stasis combined with conventional Western medicine treatment of chronic obstructive pulmonary disease in acute exacerbations can improve clinical efficacy and improve the quality of life of patients.
基金The Budget Project of Shanghai University of Traditional Chinese Medicine(2020LK083)General Project of Traditional Chinese Medicine Research of Shanghai Hongkou District Health Association(HKQ-ZYY-2021-15)+2 种基金Shanghai Natural Science Foundation Project(19ZR1451700)Shanghai Young Science and Technology Talents Sailing Program Project(20YF1445800)Chinese Medicine Research Project of Shanghai Health and Wellness Committee(2020JQ004)。
文摘Objective:To observe the clinical effect of electroacupuncture on improving polycystic ovary syndrome(PCOS)of kidney deficiency and phlegm dampness type and serum oxidative stress level.Methods:There were 68 patients with polycystic ovary syndrome were randomly divided into observation group(34 cases)and the control group(34 cases),The observation group was treated by electroacupuncture,while the control group was treated by placebo electroacupuncture.Combined with the evaluation of menstrual cycle,menstrual color,quality,quantity and TCM symptom integral index,the difference of curative effect between the two groups was observed,and the levels of peripheral blood,sex hormones,insulin resistance and oxidative stress were detected before and after treatment for 3 months.Results:The total effective rate in the observation group was better than that in the control group(P<0.01);After intervention,serum LH,FSH,E_(2) and LH/FSH in the observation group were significantly different from those before intervention.The D values of serum LH,FSH,LH/FSH and E_(2) before and after intervention were significantly different between the two groups(P<0.01);Comparing the level of androgen,the serum A4 and T in the observation group were significantly different from those before intervention(P<0.01);After intervention,there was a significant difference in serum A4 and T between the two groups(P<0.01),and there was a significant difference in D value between the two groups before and after intervention;There was a difference in insulin resistance in the observation group before and after intervention(P<0.05),and there was a significant difference in D value between the two groups before and after intervention(P<0.01);After the intervention,the serum SOD,MDA,ROS in the observation group were significantly different from those before the intervention;After intervention,the results of serum SOD and ROS in the observation group were significantly different from those in the control group;There was no significant difference in serum MDA results.There were significant differences in SOD,MDA,ROS between the two groups before and after intervention.Conclusion:Electroacupuncture can improve oxidative stress injury,insulin resistance and hyperandrogenism in PCOS patients.
文摘Objective:This study mainly explores the clinical effect of dispelling wind,eliminating lung and relieving cough combined with western medicine in the treatment of cough variant asthma.Methods:80 children with cough variant asthma accepted by our hospital from January 2018 to December 2020 were randomly selected for the study and divided into two groups.One group was the reference group(40 cases)treated with procaterol hydrochloride tablets and montelukast sodium,and the other group was the research group(40 cases)・The method of eliminating wind,eliminating lung and relieving cough was combined with procaterol hydrochloride tablets and montelukast sodium to observe and compare the curative effects of the two groups.Results:There was no significant difference in TCM symptom score and eosinophil(EOS)count between the two groups before treatment(P>0.05);After treatment,the TCM symptom scores of coughs,pharyngeal itching,expectoration,nasal congestion and nasal itching in the research group were lower than those in the reference group,and the EOS count was lower than that in the reference group(P<0.05);The effective rate of research group was higher than that of reference group(P<0.05).Conclusions:For children with cough variant asthma,Qufeng Sufei cough relieving method combined with procaterol hydrochloride and montelukast sodium can improve children's symptoms and reduce eosinophil count.
文摘Since the water supply crisis in the Taihu Lake on May 29,2007,the control of the Taihu Lake has achieved remarkable results,and the eutrophication has been greatly reduced.Existing problems are as follows:outbreaks of blue-green alga still appear seriously every year,and there is no goal to eliminate outbreaks of blue-green alga;the load into the lake greatly exceeds the environmental capacity;reed wetlands are greatly reduced;there is still the possibility of a water supply crisis;the research of eliminating outbreak of blue-green alga is weak.In this paper,the experience of controlling the Taihu Lake is summarized,and the ultimate goals of eliminating eutrophication and outbreaks of blue-green alga and restoring wetlands and biodiversity are proposed.Control measures are proposed,such as deepening the promotion of the lake chief system,establishing the lake chief system in an all-round way,and increasing applied scientific and technological efforts and capital investment.The necessity and possibility of further controlling the Taihu Lake to eliminate outbreaks of blue-green alga should be recognized.Relying on controlling eutrophication alone cannot eliminate outbreaks of blue-green alga.The point that phosphorus control is the key to eliminate outbreaks of blue-green alga is not suitable for the Taihu Lake.The integration ideas of"three types"of technologies should be innovated.The first one is"double reduction and double increase"measures to eliminate eutrophication,of which"double reduction"is to reduce external point sources and non-point sources and internal blue-green alga in sediment;for external sources,the treatment standard of sewage plants should be paid special attention to.The second one is"double algae removal"measures to salvage blue-green alga,suppresses and kills algae.The blue-green alga on the surface,middle and bottom of water should be thoroughly salvaged and eliminated from various water areas.The third one is"double restoration"measures to restore wetlands and biodiversity.The area of wetlands should be restored to the area from the 1960s to the 1970s before outbreaks of blue-green alga,and vegetation coverage reaches 25%-30%.The Taihu Lake is controlled in different waters to ensure water supply safety in water sources.Five suggestions are proposed,such as revising the"Overall Plan for the Comprehensive Treatment of Water Environment in the Taihu Lake Basin"again,incorporating the goal of eliminating outbreaks of blue-green alga in the plan,setting up this research topic,greatly improving sewage treatment standards in local legislation.
文摘该研究基于药效、血浆药物化学、网络药理学及药代动力学等方法探讨咳宁颗粒止咳祛痰的质量标志物(Q-marker)。通过浓氨水引咳和酚红排泌试验评估咳宁颗粒的止咳、祛痰药效作用。采用超高效液相色谱-四极杆-静电场轨道阱串联质谱(UHPLC-Q-Exactive Plus Orbitrap HRMS)技术分析鉴定到咳宁颗粒的原型入血成分26个。网络药理学筛选出咳宁颗粒止咳祛痰的11个潜在活性成分,涉及IL6、TLR4、STAT3等40个共同靶点,主要参与PI3K/Akt、HIF-1、EGFR等信号通路。利用药代动力学定量分析了7个原型入血成分。通过有效性、传递性、可测性将壬二酸、咖啡酸、香草醛3个化合物作为咳宁颗粒止咳祛痰的质量标志物。该研究结果对于深入明确咳宁颗粒的药效物质基础、优化质量标准以及推动其临床应用具有重要意义。
