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Experimental and Numerical Studies of Vitiated Air Effects on Hydrogen-fueled Supersonic Combustor Performance 被引量:7
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作者 LUO Feiteng SONG Wenyan +2 位作者 ZHANG Zhiqiang LI Weiqiang LI Jianping 《Chinese Journal of Aeronautics》 SCIE EI CSCD 2012年第2期164-172,共9页
This paper deals with the vitiation effects of test air on the scramjet performance in the ground combustion heated facilities. The primary goal is to evaluate the effects of H2O and CO2, the two major vitiated specie... This paper deals with the vitiation effects of test air on the scramjet performance in the ground combustion heated facilities. The primary goal is to evaluate the effects of H2O and CO2, the two major vitiated species generated by combustion heater, on hydrogen-fueled supersonic combustor performance with experimental and numerical approaches. The comparative experiments in the clean air and vitiated air are conducted by using the resistance heated direct-connected facility, with the typical Mach 4 flight conditions simulated. The H2O and CO2 species with accurately controlled contents are added to the high enthalpy clean air from resistance heater, to synthesize the vitiated air of a combustion-type heater. Typically, the contents of H2O species can be varied within the range of 3.5%-30% by mole, and 3.0%-10% for CO2 species. The total temperature, total pressure, Mach number and O2 mole fraction at the combustor entrance are well-matched between the clean air and vitiated air. The combustion experiments are completed at the fuel equivalence ratios of 0.53 and 0.42 respectively. Furthermore, three-dimensional (3D) reacting flow simulations of combustor flowpath are performed to provide insight into flow field structures and combustion chemistry details that cannot resolved by experimental instruments available. Finally, the experimental data, combined with computational results, are employed to analyze the effects of H2O and CO2 vitiated air on supersonic combustion characteristics and performance. It is concluded that H2O and CO2 contaminants can significantly inhibit the combustion induced pressure rise measured from combustor wall, and the pressure profile decreases with the increasing H2O and CO2 contents in nonlinear trend; simulation results agree well with experimental data and the overall vitiation effects are captured; direct extrapolation of the results from vitiated air to predict the performance of actual flight conditions could result in over-fueling the combustor, possible inlet un-start and inappropriate combustion mode transition. The detailed analysis and discussion are presented and the research conclusions are summarized. 展开更多
关键词 SCRAMJET HYDROGEN supersonic combustion vitiation effects experimental investigation numerical simulation
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Clinical and Experimental Study on Effect of Garlicin in Treating Angina Pectorls 被引量:2
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作者 史载祥 《Chinese Journal of Integrated Traditional and Western Medicine》 2003年第2期92-92,共1页
Objective: To observe the effect and mechanism of Garlicin in treating coronary heart disease (CHD) with angina pectoris. Methods: Clinical study: 55 patients were selected and randomized into the Garlicin group and t... Objective: To observe the effect and mechanism of Garlicin in treating coronary heart disease (CHD) with angina pectoris. Methods: Clinical study: 55 patients were selected and randomized into the Garlicin group and the nitroglycerin group. To Garlicin group, 60 mg of Garlicin was given by adding in 5% glucose solution for intravenous dripping once per day, the therapeutic course 展开更多
关键词 of in were Clinical and experimental Study on effect of Garlicin in Treating Angina Pectorls on
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EXPERIMENTAL RESEARCH ON THE ANTI-CANCER IMMUNOMODULA-TIVE EFFECT OF THE POLYSOCOHARIBE-PEPTIDE OF CORIOLUS VERSICOLOR 被引量:1
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作者 许良中 杨庆尧 +4 位作者 周金煦 陈瑞婷 李晓玉 胡玉娟 王筠默 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1989年第2期10-15,共6页
Polysocoharibe-peptide of Coriolus Versicolor (PSP) is a new anti-cancer immunomodulative drug. The present paper reports on the experimental research done with this drug. It was found that PSP had the ability to reco... Polysocoharibe-peptide of Coriolus Versicolor (PSP) is a new anti-cancer immunomodulative drug. The present paper reports on the experimental research done with this drug. It was found that PSP had the ability to recover hemolysin HC50, to increase the weight of the thymus, and increase the alexin of serum C3 and the IgG content of tumor bearing mice. FSP also significantly raised the pha-gocytic activity of macrophages in normal mice. PSP had a significant inhibitory effect on P38S and S180 cells. At the concentration of 1 mg/ml, PSP inhibited the proliferating activity of some human tumor call lines, such as SGC 7901, SPC, SLY and Mei. It had a direct toxic effect on SPC cells. PSP significantly inhibited the synthesis of nucleic acids of Ehrlich ascites carcinoma cells. In addition, PSP was antagonistic to the side effects of chemotherapy and radiotherapy. 展开更多
关键词 PSP experimentAL RESEARCH ON THE ANTI-CANCER IMMUNOMODULA-TIVE effect OF THE POLYSOCOHARIBE-PEPTIDE OF CORIOLUS VERSICOLOR SPC
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Study on effect of HDL on experimental atheroslerosis in rabbits
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《中国输血杂志》 CAS CSCD 2001年第S1期423-,共1页
关键词 HDL Study on effect of HDL on experimental atheroslerosis in rabbits
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Effect of Wave Accessibility on Lower Hybrid Wave Current Drive in Experimental Advanced Superconductor Tokamak with H-Mode Operation 被引量:1
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作者 李新霞 项农 甘春芸 《Chinese Physics Letters》 SCIE CAS CSCD 2015年第3期74-77,共4页
The effect of the wave accessibility condition on the lower hybrid cm'rent drive in the experimental advanced superconductor Tokamak (EAST) plasma with H-mode operation is studied. Based on a simplified model, a mo... The effect of the wave accessibility condition on the lower hybrid cm'rent drive in the experimental advanced superconductor Tokamak (EAST) plasma with H-mode operation is studied. Based on a simplified model, a mode conversion layer of the lower hybrid wave between the fast wave branch and the slow wave branch is proved to exist in the plasma periphery for typical EAST H-mode parameters. Under the framework of the lower hybrid wave simulation code (LSC), the wave ray trajectory and the associated current drive are calculated numerically. The results show that the wave accessibility condition plays an important role on the lower hybrid current drive in EAST plasma. For wave rays with parallel refractive index n||= 2.1 or n|| = 2.5 launched from the outside midplane, the wave rays may penetrate the core plasma due to the toroida] geometry effect, while numerous reflections of the wave ray trajectories in the plasma periphery occur. However, low current drive efficiency is obtained. Meanwhile, the wave accessibility condition is improved if a higher confined magnetic field is applied. The simulation results show that for plasma parameters under present EAST H-mode operation, a significant lower hybrid wave current drive could be obtained for the wave spectrum with peak value n|| = 2.1 if a toroidal magnetic field BT =2.5 T is applied. 展开更多
关键词 effect of Wave Accessibility on Lower Hybrid Wave Current Drive in experimental Advanced Superconductor Tokamak with H-Mode Operation
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A Fluid Experiment of Large-Scale Topography Effect on Baroclinic Wave Flows
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作者 李国庆 Robin Kung Richard L.Pfeffer 《Advances in Atmospheric Sciences》 SCIE CAS CSCD 1992年第1期17-28,共12页
The effects of topography on baroclinic wave flows are studied experimentally in a thermally driven rotating annulus of fluid.Fourier analysis and complex principal component (CPC) analysis of the experimental data sh... The effects of topography on baroclinic wave flows are studied experimentally in a thermally driven rotating annulus of fluid.Fourier analysis and complex principal component (CPC) analysis of the experimental data show that, due to topographic forcing, the flow is bimodal rather than a single mode. Under suitable imposed experimental parameters, near thermal Rossby number ROT = 0.1 and Taylor number Ta = 2.2 × 107, the large-scale topography produces low-frequency oscillation in the flow and rather long-lived flow pattern resembling blocking in the atmospheric circulation. The 'blocking' phenomenon is caused by the resonance of travelling waves and the quasi-stationary waves forced by topography.The large-scale topography transforms wavenumber-homogeneous flows into wavenumber-dispersed flows, and the dispersed flows possess lower wavenumbers. 展开更多
关键词 A Fluid experiment of Large-Scale Topography effect on Baroclinic Wave Flows
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Experimental Studies of the Doppler Effect
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作者 L. A. Pobedonostsev (Radium Institute, Pr. Shvernika 28, 194021 St. Petersburg, Russia) 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 1995年第4期115-128,共14页
Recent experimental results of the Doppler effect formula verification are reported. The moving emitters are hydrogen atoms in the excited state. The results of the work corroborate the classical not the relativistic,... Recent experimental results of the Doppler effect formula verification are reported. The moving emitters are hydrogen atoms in the excited state. The results of the work corroborate the classical not the relativistic,formula for the Doppler effect. 展开更多
关键词 Doppler effect Hydrogen atom experimental results Relativity.
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Experimental Study on the Effect of Sediment Composition Ratio on Shallow Water Delta
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作者 ZENG Can YIN Taiju +1 位作者 SONG Yakai YAN Xiujin 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2017年第S1期146-147,共2页
1 Introduction Shallow water delta in the middle-newborn Stratum Widely developed with huge oil and gas in China(Hu Shengwu et al.,2013).The control factors on the deltadevelopment like Climate,sea level,tectonic subs... 1 Introduction Shallow water delta in the middle-newborn Stratum Widely developed with huge oil and gas in China(Hu Shengwu et al.,2013).The control factors on the deltadevelopment like Climate,sea level,tectonic subsidence,sediment supply(flow,type),the geometric characteristics of the upstream river,the energy(wave, 展开更多
关键词 experimental Study on the effect of Sediment Composition Ratio on Shallow Water Delta
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Experimental study of protective effects of magnetic field on blood stored at 4℃
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《中国输血杂志》 CAS CSCD 2001年第S1期351-,共1页
关键词 experimental study of protective effects of magnetic field on blood stored at 4
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Experimental study on effect of endothelin in the proliferation and collagen synthesis of human scar-derived fibroblasts
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作者 刘德伍 《外科研究与新技术》 2003年第2期124-125,共2页
Objective To investigate the role of endothelin(ET) in the proliferation and collagen synthesis of human scar-derived fibroblasts and the moduktion of its antagonists such as nitric oxide(NO), tetrandrine ( Tet). Meth... Objective To investigate the role of endothelin(ET) in the proliferation and collagen synthesis of human scar-derived fibroblasts and the moduktion of its antagonists such as nitric oxide(NO), tetrandrine ( Tet). Methods With the cultured fibroblasts from the scarring tissue, the cell pdiferation was determined by[3H]-TdR incorporation, while the collagen synthesis was evaluated by[3H]-proline incorporation. Results The ET-1 was significantly increasing the proliferation and collagen synthesis of human scar-derived fibroblasts. The values of [3H]-TdR absorption in the 2.5 ng/ml,25 ng/ml and 100 ng/ml of ET-1 groups were 1.8 times,4 times and 4.9 times more than in the control group, respectively(P【0. 01),while the values of the [3H]-proline incorporation were 1.1 times,3.1 times and 3.8 times respectively(P【0.01). The fibroblasts, treated with 50 μg/ml of S-nitroso-N-acetyl penicillamine(SNAP), were no detectable effect on the basal level of DNA synthesis,but produced decreasing effect on the 展开更多
关键词 of experimental study on effect of endothelin in the proliferation and collagen synthesis of human scar-derived fibroblasts
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Experimental study of the effect of adipose stromal vascular fraction cells on the survival rate of fat transplantation
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作者 付冰川 《外科研究与新技术》 2011年第2期132-132,共1页
Objective To investigate the effect of adipose stromal vascular fraction cells(SVFs)on the survival rate of fat ransplantation.Methods 0.5mL autologous fat tissue was mixed with: ① DiI-labeled autologous SVFs (Group ... Objective To investigate the effect of adipose stromal vascular fraction cells(SVFs)on the survival rate of fat ransplantation.Methods 0.5mL autologous fat tissue was mixed with: ① DiI-labeled autologous SVFs (Group A);② 展开更多
关键词 rate ASCs experimental study of the effect of adipose stromal vascular fraction cells on the survival rate of fat transplantation
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An experimental study on preventive effect of continuous irrigation from peridural adhesion after laminectomy
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作者 马泉 《外科研究与新技术》 2011年第2期93-93,共1页
Objective To observe the preventive effect of continuous irrigation with sodium hyaluronate injection from peridural adhesionaminectomy.Methods Larninectomies were performed in L1,L4,L7 of 25 New Zealand rabbits respe... Objective To observe the preventive effect of continuous irrigation with sodium hyaluronate injection from peridural adhesionaminectomy.Methods Larninectomies were performed in L1,L4,L7 of 25 New Zealand rabbits respectively with 3 展开更多
关键词 An experimental study on preventive effect of continuous irrigation from peridural adhesion after laminectomy
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甘草减轻黄药子致小鼠肝毒性机制研究
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作者 黄智锋 华碧春 +4 位作者 周奕 陈雪梅 陈水钫 郑乾文 黄铮 《山西中医》 2026年第2期69-72,共4页
目的:通过microRNA测序,从基因测序水平探究甘草配伍黄药子减轻肝毒性的实验机理。方法:将实验用小鼠随机分为黄药子组、配伍组、空白对照组,每组各10只,分别连续灌胃14天后,HE染色进行肝组织形态观察,同时采用转录组学技术测序肝组织mi... 目的:通过microRNA测序,从基因测序水平探究甘草配伍黄药子减轻肝毒性的实验机理。方法:将实验用小鼠随机分为黄药子组、配伍组、空白对照组,每组各10只,分别连续灌胃14天后,HE染色进行肝组织形态观察,同时采用转录组学技术测序肝组织microRNA基因表达谱,应用软件分析表达谱提示存在差异表达的基因。结果:共筛选出具有差异表达的microRNA共93个,其中黄药子组有57个microRNA显著下调,显著上调的有36个。结论:甘草可能通过膜电位调节、突触后特化结构、离子信号活性等生物功能;以及轴突导向和Ras信号通路、细胞信号转导通路(MAPK)等多通路调节降低黄药子毒性导致肝损伤。 展开更多
关键词 黄药子 肝毒性 甘草 减毒作用 MICRORNA 实验研究
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污水毒性快速分析实验设计与环境类专业教学实践
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作者 张瑾 赵远帆 +1 位作者 崔子焱 李佳鸿 《生态毒理学报》 北大核心 2026年第1期76-84,共9页
实验教学是培养环境类专业素质高、创新能力强的复合型人才的有效途径。废水分析是环境类专业实验教学的重要内容之一,而废水毒性的快速分析将是该实验教学内容的重要补充,也是解决目前污水厂处理高毒性废水时所遇难题的技术手段之一。... 实验教学是培养环境类专业素质高、创新能力强的复合型人才的有效途径。废水分析是环境类专业实验教学的重要内容之一,而废水毒性的快速分析将是该实验教学内容的重要补充,也是解决目前污水厂处理高毒性废水时所遇难题的技术手段之一。因此,本研究设计了基于3种短生命周期生物大肠杆菌(Escherichia coli)、青海弧菌(Vibrio qinghaiensis sp.-Q67)和蛋白核小球藻(Chlorella pyrenoidosa)的快速毒性分析实验方法,并应用于安徽省某典型城市污水处理厂进出水及其8种高检出污染物(锌、镉、铜、铅、双酚、氯霉素、氧氟沙星和十二烷基苯磺酸钠)对3种指示生物的毒性效应测定,重点考察了污水中不同受试生物、不同污染物及暴露浓度和暴露时间等因素的影响,并优化了方法。目前,该实验方法已成功应用于环境生态工程专业环境生态监测课程的本科教学实践。通过该实验,使学生掌握了生物的培养、污水毒性数据的采集与数据处理方法,有效深化学生对污水生物毒性动态变化规律及监测意义的理解,引导学生理论与实践相结合,培养了学生的创新思维、实验操作能力、分析问题与解决问题以及科技论文撰写能力,增强了学生保护环境的使命感和科学报国情怀。同时,该方法还可用于环境工程、环境科学、给水排水等专业的水污染控制工程、环境监测、环境毒理学等课程的实践教学。 展开更多
关键词 污水 毒性效应 实验设计 教学实践
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舰船装备系统级电磁环境效应试验鉴定与评估思考
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作者 张勇 蔡明娟 +1 位作者 商兴华 金祖升 《舰船科学技术》 北大核心 2026年第2期174-178,共5页
新形势下,试验鉴定要加强实战电磁环境下系统级电磁环境效应试验的鉴定与评估。通过分析当前工作与新要求的差距,对系统级电磁环境效应试验鉴定进行总体设计,提出了不同平台不同场景试验要求的确定方法,明确了全寿命期舰船系统级电磁环... 新形势下,试验鉴定要加强实战电磁环境下系统级电磁环境效应试验的鉴定与评估。通过分析当前工作与新要求的差距,对系统级电磁环境效应试验鉴定进行总体设计,提出了不同平台不同场景试验要求的确定方法,明确了全寿命期舰船系统级电磁环境效应试验鉴定工作项目集,以及系统级电磁环境效应试验实施和评估方法,为面向实战、全系统和全要素的电磁环境效应试验鉴定提供指导,对提升实战环境下的舰船战斗力具有重要意义。 展开更多
关键词 舰船 系统级 电磁环境效应 试验鉴定 评估
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新闻价值如何影响体育新闻的在线传播效果?--一项基于亲近性与显要性的实验研究
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作者 方格格 胡世鑫 《体育学刊》 北大核心 2026年第1期64-74,共11页
在新媒体环境下,体育新闻如何获得更佳的传播效果?媒体常需在报道重大赛事与讲述共情故事之间做出权衡。为探究最优新闻价值配比方案,通过实验法分析“亲近性”与“显要性”两个新闻价值要素对新闻可信度感知及在线传播效果的影响。结... 在新媒体环境下,体育新闻如何获得更佳的传播效果?媒体常需在报道重大赛事与讲述共情故事之间做出权衡。为探究最优新闻价值配比方案,通过实验法分析“亲近性”与“显要性”两个新闻价值要素对新闻可信度感知及在线传播效果的影响。结果显示,虽然亲近性与显要性都能提升体育新闻的传播效果,但二者的失衡会带来负面效果。即当一则体育新闻显要性很高但亲近性不足时,受众的可信度感知会显著降低,进而抑制其互动意愿。新闻可信度在此过程中起到关键的中介作用。基于新闻价值配比视角,通过实证分析把握体育新闻在线传播的最佳实践组合规律,研究认为体育新闻记者在追求重大主题报道的同时,需通过生动的细节与个人化的叙事来增强报道亲近性,以此夯实新闻的公信力根基,激发广泛的受众情感共鸣,从而有效传递体育赛事所蕴含的多元社会人文价值。 展开更多
关键词 体育新闻 新闻价值 在线传播效果 实验研究 可信度感知
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水系灭火剂抑制电动自行车锂电池火灾的实验研究
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作者 王晓冬 胡玢 +1 位作者 余章龙 谢鹏 《安全》 2026年第2期30-34,共5页
为探究水系灭火剂对电动自行车锂电池火灾的抑制效能,本文以磷酸铁锂电池模组为对象,模拟充电柜热失控场景开展灭火实验。通过监测灭火过程温度变化、明火熄灭时间及复燃情况,系统评估灭火有效性。结果显示:水系灭火剂可快速降低磷酸铁... 为探究水系灭火剂对电动自行车锂电池火灾的抑制效能,本文以磷酸铁锂电池模组为对象,模拟充电柜热失控场景开展灭火实验。通过监测灭火过程温度变化、明火熄灭时间及复燃情况,系统评估灭火有效性。结果显示:水系灭火剂可快速降低磷酸铁锂电池模组燃烧产生的热量,实现5 min内灭火、灭火后30 min不复燃的防控目标;但当水系灭火剂仅作用于锂电池模组金属外壳而未直接接触电芯时,灭火时间显著延长。研究结果可为水系灭火剂在锂电池模组火灾防控中的应用提供实验依据。 展开更多
关键词 水系灭火剂 电动自行车 锂电池火灾 灭火效能 实验
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石菖蒲在神经系统疾病中的应用研究进展
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作者 宋叶彤 史正刚 +2 位作者 高旅 李征 刘莉霞 《中国中医药信息杂志》 2026年第2期160-164,F0003,共6页
神经系统疾病表现为认知、运动、感觉障碍等,发病机制复杂,严重影响患者身心健康。石菖蒲具有开窍豁痰、醒神益脑、化湿开胃等功效,可多靶点、多通路、多途径发挥对中枢神经系统的保护作用。本文梳理石菖蒲及其有效成分治疗抑郁症、癫... 神经系统疾病表现为认知、运动、感觉障碍等,发病机制复杂,严重影响患者身心健康。石菖蒲具有开窍豁痰、醒神益脑、化湿开胃等功效,可多靶点、多通路、多途径发挥对中枢神经系统的保护作用。本文梳理石菖蒲及其有效成分治疗抑郁症、癫痫、阿尔茨海默病、帕金森病、抽动障碍等神经系统疾病的研究进展,石菖蒲富含挥发油、黄酮类等多种活性成分,可通过调控炎症因子、调节神经递质、抗氧化应激、保护血脑屏障等机制发挥疗效,可为其基础研究及临床应用提供研究思路。 展开更多
关键词 石菖蒲 神经系统疾病 实验研究 药理作用 综述
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复方黄芪汤调控VEGF-AKT信号通路抑制血管新生的抗肺癌机制
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作者 钟怡 尹春燕 +6 位作者 廉源沛 蔡佳丽 王地均 聂雪珂 刘产明 申春悌 颜晓静 《中药新药与临床药理》 北大核心 2026年第1期70-81,共12页
目的基于网络药理学、分子对接及动物实验验证揭示复方黄芪汤抗肺癌分子机制。方法通过网络药理学预测复方黄芪汤抗肺癌的核心成分、靶基因和关键信号通路并进行分子对接;构建Lewis荷瘤小鼠模型,设立空白对照组、荷瘤对照组、Pd^(-1)抗... 目的基于网络药理学、分子对接及动物实验验证揭示复方黄芪汤抗肺癌分子机制。方法通过网络药理学预测复方黄芪汤抗肺癌的核心成分、靶基因和关键信号通路并进行分子对接;构建Lewis荷瘤小鼠模型,设立空白对照组、荷瘤对照组、Pd^(-1)抗体组(10 mg·kg^(-1))及复方黄芪汤低、高剂量组(30、60 g·kg^(-1)),统计肿瘤质量和抑瘤率;苏木精-伊红(HE)染色法检测肿瘤及肺组织病理变化,免疫组化法检测肿瘤组织中微血管密度蛋白(FactorⅧ)表达,酶联免疫吸附测定法(ELISA)检测血清中血管内皮生长因子(VEGF)含量,Western Blot法检测肿瘤组织中磷酸化蛋白激酶B(p-AKT)和蛋白激酶B(AKT)蛋白表达。结果从数据库筛选出复方黄芪汤活性成分56个,交集靶点181个,核心靶点9个,对应活性成分8个;基因本体(GO)分析及基因组百科全书(KEGG)信号通路富集分析结果显示,其主要通过调控PI3K-AKT、JAK-STAT、EGFR酪氨酸激酶抑制剂耐药等信号通路抗肺癌。分子对接提示黄芪紫檀烷苷与VEGF及山柰酚、熊竹素、异鼠李素与蛋白激酶Bα(AKT1)具有良好结合力。动物实验验证结果显示,与荷瘤对照组比较,复方黄芪汤高剂量组能显著抑制肿瘤生长,降低肿瘤质量(P<0.01)。HE染色结果显示肿瘤细胞的细胞核大且核分裂相明显,而复方黄芪汤干预后肿瘤细胞排列松散,细胞间隙大,细胞活跃度低。与荷瘤对照组比较,复方黄芪汤高剂量组能显著降低FactorⅧ水平(P<0.001)及VEGF含量(P<0.01);Western Blot结果显示其能显著下调p-AKT/AKT比值(P<0.01)。结论复方黄芪汤可能通过干预VEGF-AKT信号通路,抑制肿瘤血管生成,进而发挥抑瘤作用。 展开更多
关键词 复方黄芪汤 肺癌 抑瘤网络药理学 VEGF-AKT信号通路 血管新生 实验验证 小鼠
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清解扶正颗粒对5-氟尿嘧啶所致大鼠心脏损伤保护作用研究
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作者 刘云 贾沛芝 +1 位作者 刘锦洪 林久茂 《山西中医》 2026年第2期65-68,共4页
目的:观察5-氟尿嘧啶(5-FU)对大鼠心脏的影响以及清解扶正颗粒对5-氟尿嘧啶所致大鼠心脏损伤的保护作用。方法:采用18只Wistar大鼠,随机分为对照组、模型组、治疗组,每组各6只。对照组予生理盐水注射,模型组予5-FU腹腔注射3周,治疗组在5... 目的:观察5-氟尿嘧啶(5-FU)对大鼠心脏的影响以及清解扶正颗粒对5-氟尿嘧啶所致大鼠心脏损伤的保护作用。方法:采用18只Wistar大鼠,随机分为对照组、模型组、治疗组,每组各6只。对照组予生理盐水注射,模型组予5-FU腹腔注射3周,治疗组在5-FU腹腔注射同时给予清解扶正颗粒灌胃3周。通过HE染色观察心脏的病理形态变化;TUNEL检测心脏组织的凋亡;免疫组化检测Ki-67、Bax、Bcl-2蛋白表达;透射电镜与MitoMark RedⅠ染色评估线粒体的损伤情况。结果:与对照组比较,HE染色结果显示模型组大鼠心脏组织出现明显损伤,治疗组大鼠较模型组心脏组织间隙减小,细胞核排列紊乱损伤程度缓解。与对照组比较,TUNEL结果显示模型组大鼠心脏组织凋亡数量增加,治疗组凋亡数量较模型组有所减少。免疫组化结果显示,与对照组比较,模型组增殖标志物Ki-67、抗凋亡蛋白Bcl-2表达降低,促凋亡蛋白Bax表达升高,治疗组较模型组Ki-67、Bcl-2蛋白表达增加,Bax蛋白表达降低(P﹤0.05)。透射电镜及MitoMark RedⅠ染色显示,与对照组比较,模型组大鼠线粒体明显减少,MitoMark RedⅠ荧光强度变弱,治疗组大鼠的线粒体数量与荧光强度较模型组均有有所提升。结论:5-FU对大鼠心脏具有明显的毒性作用,清解扶正颗粒能够减轻5-FU引起的心脏损伤,对心脏具有保护作用。 展开更多
关键词 心脏损伤 5-氟尿嘧啶 副作用 清解扶正颗粒 实验研究
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