Electron enregy loss spectroscopy (EELS) and energy filtering transmission electron microscopy (EFTEM) investigation on 2.25Cr-lMo steel was carried out to understand the nature of evolution of secondary carbides. The...Electron enregy loss spectroscopy (EELS) and energy filtering transmission electron microscopy (EFTEM) investigation on 2.25Cr-lMo steel was carried out to understand the nature of evolution of secondary carbides. The filtered images obtained from two different ageing treatments indicate that the steel evolves to a more stable carbide namely M23C6 in comparison to M2C. Microchemical information was generated from EELS spectra. Suitable choice for estimating the microchemical state was discussed. To evaluate the behaviour of ageing an elemental ratio of Fe to Cr is employed.展开更多
The Yangtze River estuary is the main production area of Anguilla japonica in China,as well as the only existing fishery area for adult eels.Japanese eels are distributed in the main rivers and many tributaries from t...The Yangtze River estuary is the main production area of Anguilla japonica in China,as well as the only existing fishery area for adult eels.Japanese eels are distributed in the main rivers and many tributaries from the Yangtze River estuary to the upper Jinsha River,which extend to nearly 3 000 km.However,their migration behaviors remain relatively unknown.We analyzed the biological characteristics of 153 specimens of silver eels collected from the Jingjiang section of Yangtze River（31o30＇N,120o42＇E） between September and November,2008,and tested the sagittal Sr/Ca ratios of 27 specimens.Among the 153 specimens examined,85 were female and 68 were male,which translated to a female-male ratio of 1：0.8.The ages of the female specimens ranged from 3 to 7 a（average 5.52） with an average total length（TL） of（669±80） mm,average body weight（BW） of（555±229）g,average condition factor of 1.77±0.22,and average gonad somatic index（GSI） of 1.32±0.31.The ages of the males ranged from 3 to 5 a（average：4.38） with an average TL of（518±51） mm,average BW of（234±76） g,average condition factor of 1.62±0.18,and average GSI of 0.21±0.11.All biological parameters of females were significantly larger than those of the male specimens（P0.05）.According to the average Sr/Ca ratio（7.99±1.05） ×10-3 of the elver mark of sagitta,17 individuals（62.96%） were river eels and 10 individuals（37.04%） were estuarine eels.Of 16 females,13 individuals（81.25%） were river eels and 3 were estuarine eels,while of 11 males,36.36% were river eels and 63.64% were estuarine eels.The analysis on Sr/Ca ratios for every growth layer group（GLG） indicated there were no significant differences between second-age males and females.However,significant differences were observed between the third-age,fourth-age,and migration-age male and female specimens.This was likely related to the fact that second-age eels of both sexes stay in the same inhabitation waters;however,as they grow older,they move to different areas.展开更多
Nucleoporins(Nups)are important components of nuclear pore complexes(NPCs).NPCs control gene expression,cells proliferation and differentiation by mediating exchange of cellular signal molecules on both nuclear and cy...Nucleoporins(Nups)are important components of nuclear pore complexes(NPCs).NPCs control gene expression,cells proliferation and differentiation by mediating exchange of cellular signal molecules on both nuclear and cytoplasmic sides.Using subtractive screening,3'end fragment of Nup93 from the testis cDNA library of the rice field eel was obtained.Full-length cDNA of the gene was further cloned by degenerate PCR and 5'RACE methods.Sequence analysis indicated that the homology of the rice field eel Nup93 were 36.5%with yeast Nic96,94.6%and 90.5%with Nup93 of zebrafish and human,respectively.Phylogenetic analysis showed that the rice field eel Nup93 fits with Nup93 of the other fishes.Real-time PCR result showed that expression of Nup93 in gonads and kidney were much higher than in other tissues,and different expression quantities among gonads of three sexes were also observed,suggesting that Nup93 may involve in gonad development.展开更多
[Objective] The aim was to find a suitable weaning regime, including weaning period and weaning feed, for Angui//a mormorata glass eels. [Method] A weaning processing experiment was conducted indoor in fiberglass tank...[Objective] The aim was to find a suitable weaning regime, including weaning period and weaning feed, for Angui//a mormorata glass eels. [Method] A weaning processing experiment was conducted indoor in fiberglass tanks, two kinds of artificial feeds （eel grower powder feed and eel grower piece feed） were selected as weaning diets against a control group fed on natural food （minced octopus flesh）, two weaning periods, 5 and 15 d, were introduced in weaning experiment. [Result] The growth rate and survival rate of the groups with 15-day＇s weaning period were higher than that of the groups with 5-day＇s weaning period, revealing that a 20% increase every 3 d from 0% to 100% in the proportion of grower paste in the minced octopus flesh during weaning is suitable for A. mormorata glass eels. [Conclusion] A. mormorata glass eels can be weaned from natural food to the commer- cially available eel grower feeds over a certain weaning period, and of the artificial feeds with same ingredients and nutrient, A. mormorata glass eels accepted powder feed more readily over 28 d than piece feed.展开更多
American eels are declining throughout their range requiring a better understanding of physiological requirements of all life stages and optimal conditions for laboratory rearing and aquaculture. American glass eels (...American eels are declining throughout their range requiring a better understanding of physiological requirements of all life stages and optimal conditions for laboratory rearing and aquaculture. American glass eels (Anguilla rostrata) were housed for 3 weeks at 14°C, 18°C, 22°C, or 26°C to determine optimal juvenile rearing temperature in the laboratory. All treatments exhibited weight gain over the course of the study except the 14°C treatment;however, there were only marginal differences in final weight between the 18°C and 14°C treatments and no differences in length. Variation in length and weight generally increased as temperature increased with significant differences in the standard error of weight between 14°C and the 22°C and 26°C treatments and between 18°C and 26°C. Mortality was significantly greater than expected by chance at 26°C?(7 deaths) and no mortality was observed at 14°C. Body condition (based on the residuals from the weight-length relationships), conversely, was lowest in the 14°C treatment. Considering all response variables, optimal laboratory rearing conditions were observed between 18°C - 22°C. Within a week of experimentation, evidence of gas bubble disease was observed and by completion noted in all treatments except at 14°C, likely as a function of decreased gas solubility at warmer temperatures. Levels of total gas pressure (103% - 108%) and Δp (28 - 54 mm Hg) values may account for the gas bubbles observed.展开更多
A review of our experience in range of electron spectroscopy of the physical vapor-phase deposition and growth of single- and multilayer nanostructures with atomic scale interfaces is presented. The foundation of an i...A review of our experience in range of electron spectroscopy of the physical vapor-phase deposition and growth of single- and multilayer nanostructures with atomic scale interfaces is presented. The foundation of an innovative methodology for the combined AES-EELS analysis of layered nanostructures is developed. The methodology includes: 1) determination of the composition, thickness, and the mechanism of phase transitions in nanocoatings under the probing depth most appropriated for the range of film thickness 1 - 10 ML;2) quantitative iteration Auger-analysis of the composition, thickness and growth mechanism of nanocoating;3) structural and phase analysis of nanocoatings with use of the analysis of position, shape and energy of the plasmon EELS peak and with subtracting the contribution from the substrate;4) analysis of phase transitions with use of the shift of the plasmon Auger-satellite and 5) non-destructive profiling of the composition of nanocoatings over depth with use of a dependence of the intensity and energy of EELS peaks on the value of the primary electron energy.展开更多
The present study aimed at making a rational usage for European eel bone by-products by preparing Europen eel bone peptide chelated calcium(EBPC-Ca).Nutritional properties and bioactivity of EBPC-Ca were evaluated.Res...The present study aimed at making a rational usage for European eel bone by-products by preparing Europen eel bone peptide chelated calcium(EBPC-Ca).Nutritional properties and bioactivity of EBPC-Ca were evaluated.Results showed that nutritional properties of calcium ions will cause intra-and inter-molecular folding and aggregation of peptide to uniformly form EBPC-Ca chelate.The chelated compound of EBPC and calcium ion triggered a strong apoptosis in heterogeneous human epithelial colorectal adenocarcinoma(Caco-2)in concentration-and time-dependent manners.Western blot analysis revealed that the EBPC-Ca induced apoptosis may be the result of a blocked autophagy flux through mitochondrial-dependent pathway.Additionally,the increase in FGF-23 protein expression inhibited the absorption of calcium ions and alleviated cell apoptosis.It was also found that the cell apoptosis occurs with significant increases in the levels of reactive oxygen species(ROS)and Ca^(2+)in the cells,indicating the anti-tumor potential of EBPC-Ca may involve multiple channels.展开更多
文摘Electron enregy loss spectroscopy (EELS) and energy filtering transmission electron microscopy (EFTEM) investigation on 2.25Cr-lMo steel was carried out to understand the nature of evolution of secondary carbides. The filtered images obtained from two different ageing treatments indicate that the steel evolves to a more stable carbide namely M23C6 in comparison to M2C. Microchemical information was generated from EELS spectra. Suitable choice for estimating the microchemical state was discussed. To evaluate the behaviour of ageing an elemental ratio of Fe to Cr is employed.
基金supported by the Chinese National Science Foundation under the Grant(30771650)the Key Programs of Science and Technology Commission Foundation of Shanghai(08391910200)+3 种基金Special Fund for Agroscientific Research in the Public Interest(201203065)Research Fund for the Doctoral Program of Higher Education of China(20070264001)E-class Programs of Shanghai Municipal Education Commission(E03009)the Key Subject Construction of Shanghai(S30701)
文摘The Yangtze River estuary is the main production area of Anguilla japonica in China,as well as the only existing fishery area for adult eels.Japanese eels are distributed in the main rivers and many tributaries from the Yangtze River estuary to the upper Jinsha River,which extend to nearly 3 000 km.However,their migration behaviors remain relatively unknown.We analyzed the biological characteristics of 153 specimens of silver eels collected from the Jingjiang section of Yangtze River（31o30＇N,120o42＇E） between September and November,2008,and tested the sagittal Sr/Ca ratios of 27 specimens.Among the 153 specimens examined,85 were female and 68 were male,which translated to a female-male ratio of 1：0.8.The ages of the female specimens ranged from 3 to 7 a（average 5.52） with an average total length（TL） of（669±80） mm,average body weight（BW） of（555±229）g,average condition factor of 1.77±0.22,and average gonad somatic index（GSI） of 1.32±0.31.The ages of the males ranged from 3 to 5 a（average：4.38） with an average TL of（518±51） mm,average BW of（234±76） g,average condition factor of 1.62±0.18,and average GSI of 0.21±0.11.All biological parameters of females were significantly larger than those of the male specimens（P0.05）.According to the average Sr/Ca ratio（7.99±1.05） ×10-3 of the elver mark of sagitta,17 individuals（62.96%） were river eels and 10 individuals（37.04%） were estuarine eels.Of 16 females,13 individuals（81.25%） were river eels and 3 were estuarine eels,while of 11 males,36.36% were river eels and 63.64% were estuarine eels.The analysis on Sr/Ca ratios for every growth layer group（GLG） indicated there were no significant differences between second-age males and females.However,significant differences were observed between the third-age,fourth-age,and migration-age male and female specimens.This was likely related to the fact that second-age eels of both sexes stay in the same inhabitation waters;however,as they grow older,they move to different areas.
基金Supported by the National Natural Science Foundation of China,the National Key Basic Research Project(No.2004CB117400)the Program for New Century Excellent Talents in Universitythe Key Project of Chinese Ministry of Education.
文摘Nucleoporins(Nups)are important components of nuclear pore complexes(NPCs).NPCs control gene expression,cells proliferation and differentiation by mediating exchange of cellular signal molecules on both nuclear and cytoplasmic sides.Using subtractive screening,3'end fragment of Nup93 from the testis cDNA library of the rice field eel was obtained.Full-length cDNA of the gene was further cloned by degenerate PCR and 5'RACE methods.Sequence analysis indicated that the homology of the rice field eel Nup93 were 36.5%with yeast Nic96,94.6%and 90.5%with Nup93 of zebrafish and human,respectively.Phylogenetic analysis showed that the rice field eel Nup93 fits with Nup93 of the other fishes.Real-time PCR result showed that expression of Nup93 in gonads and kidney were much higher than in other tissues,and different expression quantities among gonads of three sexes were also observed,suggesting that Nup93 may involve in gonad development.
基金Supported by Xiamen Marine Research and Development Institute [K10102(1)]~~
文摘[Objective] The aim was to find a suitable weaning regime, including weaning period and weaning feed, for Angui//a mormorata glass eels. [Method] A weaning processing experiment was conducted indoor in fiberglass tanks, two kinds of artificial feeds （eel grower powder feed and eel grower piece feed） were selected as weaning diets against a control group fed on natural food （minced octopus flesh）, two weaning periods, 5 and 15 d, were introduced in weaning experiment. [Result] The growth rate and survival rate of the groups with 15-day＇s weaning period were higher than that of the groups with 5-day＇s weaning period, revealing that a 20% increase every 3 d from 0% to 100% in the proportion of grower paste in the minced octopus flesh during weaning is suitable for A. mormorata glass eels. [Conclusion] A. mormorata glass eels can be weaned from natural food to the commer- cially available eel grower feeds over a certain weaning period, and of the artificial feeds with same ingredients and nutrient, A. mormorata glass eels accepted powder feed more readily over 28 d than piece feed.
文摘American eels are declining throughout their range requiring a better understanding of physiological requirements of all life stages and optimal conditions for laboratory rearing and aquaculture. American glass eels (Anguilla rostrata) were housed for 3 weeks at 14°C, 18°C, 22°C, or 26°C to determine optimal juvenile rearing temperature in the laboratory. All treatments exhibited weight gain over the course of the study except the 14°C treatment;however, there were only marginal differences in final weight between the 18°C and 14°C treatments and no differences in length. Variation in length and weight generally increased as temperature increased with significant differences in the standard error of weight between 14°C and the 22°C and 26°C treatments and between 18°C and 26°C. Mortality was significantly greater than expected by chance at 26°C?(7 deaths) and no mortality was observed at 14°C. Body condition (based on the residuals from the weight-length relationships), conversely, was lowest in the 14°C treatment. Considering all response variables, optimal laboratory rearing conditions were observed between 18°C - 22°C. Within a week of experimentation, evidence of gas bubble disease was observed and by completion noted in all treatments except at 14°C, likely as a function of decreased gas solubility at warmer temperatures. Levels of total gas pressure (103% - 108%) and Δp (28 - 54 mm Hg) values may account for the gas bubbles observed.
文摘A review of our experience in range of electron spectroscopy of the physical vapor-phase deposition and growth of single- and multilayer nanostructures with atomic scale interfaces is presented. The foundation of an innovative methodology for the combined AES-EELS analysis of layered nanostructures is developed. The methodology includes: 1) determination of the composition, thickness, and the mechanism of phase transitions in nanocoatings under the probing depth most appropriated for the range of film thickness 1 - 10 ML;2) quantitative iteration Auger-analysis of the composition, thickness and growth mechanism of nanocoating;3) structural and phase analysis of nanocoatings with use of the analysis of position, shape and energy of the plasmon EELS peak and with subtracting the contribution from the substrate;4) analysis of phase transitions with use of the shift of the plasmon Auger-satellite and 5) non-destructive profiling of the composition of nanocoatings over depth with use of a dependence of the intensity and energy of EELS peaks on the value of the primary electron energy.
基金supported by National Natural Science Foundation of China(NSFC,Grant No.31801459,Grant No.32072209)China Postdoctoral Science Foundation Funded Project(2020M682073)+1 种基金the Science and Technology General Projects of Fujian Province(2019J01393)Program of Innovative Research Team in Science and Technology in Fujian Province University([2020]12)。
文摘The present study aimed at making a rational usage for European eel bone by-products by preparing Europen eel bone peptide chelated calcium(EBPC-Ca).Nutritional properties and bioactivity of EBPC-Ca were evaluated.Results showed that nutritional properties of calcium ions will cause intra-and inter-molecular folding and aggregation of peptide to uniformly form EBPC-Ca chelate.The chelated compound of EBPC and calcium ion triggered a strong apoptosis in heterogeneous human epithelial colorectal adenocarcinoma(Caco-2)in concentration-and time-dependent manners.Western blot analysis revealed that the EBPC-Ca induced apoptosis may be the result of a blocked autophagy flux through mitochondrial-dependent pathway.Additionally,the increase in FGF-23 protein expression inhibited the absorption of calcium ions and alleviated cell apoptosis.It was also found that the cell apoptosis occurs with significant increases in the levels of reactive oxygen species(ROS)and Ca^(2+)in the cells,indicating the anti-tumor potential of EBPC-Ca may involve multiple channels.
