Sechium edule(chayote)is an important vegetable crop belonging to the Cucurbitaceae family.To decipher the chayote genome,a highquality chromosome-level chayote genome was obtained by genome sequencing and bioinformat...Sechium edule(chayote)is an important vegetable crop belonging to the Cucurbitaceae family.To decipher the chayote genome,a highquality chromosome-level chayote genome was obtained by genome sequencing and bioinformatic analysis.The total length was612.91 Mb,and 25755 genes were detected in the chayote genome.The contig N50 was more than 20.01 Mb,and the scaffold N50 was over47.11 Mb.Of the genome,60.35%were composed of repetitive sequences,and 31.18%of genome sequences belonged to long-terminal repeats.A global alignment of homologous regions in chayote and other Cucurbitaceae plant genomes was constructed using grape as a reference.Based on this genome-wide and global alignment map,researchers can easily identify homologous collinear genes of the studied genomes in most Cucurbitaceae species.Twenty-five chayote accessions were divided into two subgroups based on phylogenetic tree,population structure analysis,and principal component analysis using genome re-sequencing data.The chayote genome,re-sequencing dataset,and comprehensive genomic analysis will accelerate comparative and functional genomic analysis of chayote and other Cucurbitaceae species in the future.展开更多
This study investigated the efficiency of activated carbon from Pangium edule shells for removing phosphate from aqueous solution.The adsorption capacity of the synthesized activated carbon was determined to be 19.839...This study investigated the efficiency of activated carbon from Pangium edule shells for removing phosphate from aqueous solution.The adsorption capacity of the synthesized activated carbon was determined to be 19.8392 mg g−1.Various isotherm models were used to analyze the adsorption process,Henry,Freundlich,SIP,and Halsey isotherm fitting showed r2 values close to 1.0.These isotherms indicated a combination of physisorption and chemisorption mechanisms,with heterogeneity and multilayer formation playing important roles.A pseudo-second-order model described the adsorption kinetics well,suggesting chemisorption as the dominant mechanism with an r2 value of 1.0 and a rate constant k_(2) of 1.2550 min^(-1).The optimization was carried out using central composite design(CCD)using 3 factors(contact time(minutes),adsorbent dosage(mg),and initial phosphate concentration(ppm))with 3 levels.The CCD output was analyzed using response surface methodology(RSM)to obtain the optimum level of each factor.A contact time of one to two hours and an adsorbent dosage of more than 80 mg was recommended.Optimal removal was achieved at initial phosphate concentrations between 800 and 1150 ppm.Morphological analysis using scanning electron microscope(SEM)showed a highly irregular surface structure of activated carbon,while X-ray Diffraction(XRD)patterns indicated the presence of amorphous carbon.Fourier Transform Infrared(FTIR)analysis identified functional groups contributing to the adsorption process and Energy Dispersive X-ray Spectroscopy(EDX)analysis confirmed the presence of phosphate on the carbon surface after adsorption.In conclusion,activated carbon from P.edule shells has significant potential in phosphate removal,with a combination of high adsorption capacity,effective adsorption mechanism,and favorable kinetics,making it a promising material for water treatment.展开更多
Objective:To evaluate the anti-proliferative and apoptogenic activity of ethyl acetate extract from the leaves of Memecylon edule(EtAc-LME) in MKN-74,NUGC gastric cancer cells and non cancerous gastric mucous cells(GE...Objective:To evaluate the anti-proliferative and apoptogenic activity of ethyl acetate extract from the leaves of Memecylon edule(EtAc-LME) in MKN-74,NUGC gastric cancer cells and non cancerous gastric mucous cells(GES-1),and to explore the mechanism of EtAc-LME induced apoptosis.Methods:The mechanism of EtAc-LME induced apoptosis was explored by analysing the activation of pro-caspases,PARP cleavage,expression of cytochrome-c (Cyt-c) was determined by western blotting,mRNA expression of Bcl-2,Bax by RT-PCR,loss of mitochondrial potential using DiOC6 dye,annexin binding assay and its influence on cell cycle arrest by flow cytometry.Results:The results indicated that EtAc-LME inhibited the gastric cancer cell growth in dose-dependent manner and cytotoxicity was more towards the gastric cancer cells(NUCC and MKN-74) compared to normal gastric cells(GES-1),suggesting more specific cytotoxicity to the malignant cells.Over expression of Cyt-c and subsequent activation of caspases-3 and down regulation of Bcl-2 and loss in mitochondrial potential in EtAc-LME treated MKN-74 and NUGC cells suggested that EtAc-LME induced apoptosis by mitochondrial dependent pathway.Conclusions:The present findings suggest that ethyl acetate extract of Memecylon edule induces apoptosis selectively in gastric cancer cells emphasizing the importance of this traditional medicine for its potential in the treatment of gastric cancer.展开更多
基金supported by the National Natural Science Foundation of China Project(Grant No.32260097)the National Guidance Foundation for Local Science and Technology Development of China(Grant No.[2023]009)the Natural Science Foundation for Distinguished Young Scholars of Hebei(Grant No.C2022209010)。
文摘Sechium edule(chayote)is an important vegetable crop belonging to the Cucurbitaceae family.To decipher the chayote genome,a highquality chromosome-level chayote genome was obtained by genome sequencing and bioinformatic analysis.The total length was612.91 Mb,and 25755 genes were detected in the chayote genome.The contig N50 was more than 20.01 Mb,and the scaffold N50 was over47.11 Mb.Of the genome,60.35%were composed of repetitive sequences,and 31.18%of genome sequences belonged to long-terminal repeats.A global alignment of homologous regions in chayote and other Cucurbitaceae plant genomes was constructed using grape as a reference.Based on this genome-wide and global alignment map,researchers can easily identify homologous collinear genes of the studied genomes in most Cucurbitaceae species.Twenty-five chayote accessions were divided into two subgroups based on phylogenetic tree,population structure analysis,and principal component analysis using genome re-sequencing data.The chayote genome,re-sequencing dataset,and comprehensive genomic analysis will accelerate comparative and functional genomic analysis of chayote and other Cucurbitaceae species in the future.
基金funded by DRPM,Institut Teknologi Sepuluh Nopember,under Research Grant No.1284/PKS/ITS/2024,dated 29 February 2024.
文摘This study investigated the efficiency of activated carbon from Pangium edule shells for removing phosphate from aqueous solution.The adsorption capacity of the synthesized activated carbon was determined to be 19.8392 mg g−1.Various isotherm models were used to analyze the adsorption process,Henry,Freundlich,SIP,and Halsey isotherm fitting showed r2 values close to 1.0.These isotherms indicated a combination of physisorption and chemisorption mechanisms,with heterogeneity and multilayer formation playing important roles.A pseudo-second-order model described the adsorption kinetics well,suggesting chemisorption as the dominant mechanism with an r2 value of 1.0 and a rate constant k_(2) of 1.2550 min^(-1).The optimization was carried out using central composite design(CCD)using 3 factors(contact time(minutes),adsorbent dosage(mg),and initial phosphate concentration(ppm))with 3 levels.The CCD output was analyzed using response surface methodology(RSM)to obtain the optimum level of each factor.A contact time of one to two hours and an adsorbent dosage of more than 80 mg was recommended.Optimal removal was achieved at initial phosphate concentrations between 800 and 1150 ppm.Morphological analysis using scanning electron microscope(SEM)showed a highly irregular surface structure of activated carbon,while X-ray Diffraction(XRD)patterns indicated the presence of amorphous carbon.Fourier Transform Infrared(FTIR)analysis identified functional groups contributing to the adsorption process and Energy Dispersive X-ray Spectroscopy(EDX)analysis confirmed the presence of phosphate on the carbon surface after adsorption.In conclusion,activated carbon from P.edule shells has significant potential in phosphate removal,with a combination of high adsorption capacity,effective adsorption mechanism,and favorable kinetics,making it a promising material for water treatment.
文摘Objective:To evaluate the anti-proliferative and apoptogenic activity of ethyl acetate extract from the leaves of Memecylon edule(EtAc-LME) in MKN-74,NUGC gastric cancer cells and non cancerous gastric mucous cells(GES-1),and to explore the mechanism of EtAc-LME induced apoptosis.Methods:The mechanism of EtAc-LME induced apoptosis was explored by analysing the activation of pro-caspases,PARP cleavage,expression of cytochrome-c (Cyt-c) was determined by western blotting,mRNA expression of Bcl-2,Bax by RT-PCR,loss of mitochondrial potential using DiOC6 dye,annexin binding assay and its influence on cell cycle arrest by flow cytometry.Results:The results indicated that EtAc-LME inhibited the gastric cancer cell growth in dose-dependent manner and cytotoxicity was more towards the gastric cancer cells(NUCC and MKN-74) compared to normal gastric cells(GES-1),suggesting more specific cytotoxicity to the malignant cells.Over expression of Cyt-c and subsequent activation of caspases-3 and down regulation of Bcl-2 and loss in mitochondrial potential in EtAc-LME treated MKN-74 and NUGC cells suggested that EtAc-LME induced apoptosis by mitochondrial dependent pathway.Conclusions:The present findings suggest that ethyl acetate extract of Memecylon edule induces apoptosis selectively in gastric cancer cells emphasizing the importance of this traditional medicine for its potential in the treatment of gastric cancer.
