Quanming Lu,Prosessor,Changjiang Distinguished Professor,National Science Fund for Distinguished Young Scholars.Rongbin Zhou,Prosessor,Changjiang Distinguished Professor,National Science Fund for Distinguished Young S...Quanming Lu,Prosessor,Changjiang Distinguished Professor,National Science Fund for Distinguished Young Scholars.Rongbin Zhou,Prosessor,Changjiang Distinguished Professor,National Science Fund for Distinguished Young Scholars,Deputy Director,Life Sciences and Medicine,USTC.展开更多
Han-Ping Shi,M.D.,Ph.D.,FACS,Professor of Surgery,Oncology and Nutrition.He is the current Chairman of the Department of GI Surgery/Clinical Nutrition at Beijing Shijitan Hospital,Capital Medical University,Beijing,Ch...Han-Ping Shi,M.D.,Ph.D.,FACS,Professor of Surgery,Oncology and Nutrition.He is the current Chairman of the Department of GI Surgery/Clinical Nutrition at Beijing Shijitan Hospital,Capital Medical University,Beijing,China.Dr.Shi received his M.D.degree at the School of Medicine,Wuhan University,Wuhan,and his Ph.D.degree at the Second Military Medical University,Shanghai,China.展开更多
The Editorial Office of Water Science and Engineering would like to express their sincere appreciation to the academic editors including Prof.Carlo Gualtieri from University of Napoli Federico Ⅱ,Italy,Prof.Guo-qing W...The Editorial Office of Water Science and Engineering would like to express their sincere appreciation to the academic editors including Prof.Carlo Gualtieri from University of Napoli Federico Ⅱ,Italy,Prof.Guo-qing Wang and Prof.Zhong-zhi Fu from Nanjing Hydraulic Research Institute,China,and Prof.Yan-hui Ao,Prof.Ching-sheng Huang,Prof.Guang-qiu Jin,Prof.Bin Xu,Prof.Sai-yu Yuan,Prof.Zeng Zhou,Prof.Bo Chen,and Prof.Da-wei Guan from Hohai Uiversity,China,for their great effort and contribution to WSE in the year 2024.展开更多
Editor-in-Chief:Xinhe Bao Professor,President of University of Science and Technology of China,Academician of Chinese Academy of Sciences(CAS),Academician of The World Academy of Sciences(TWAS),Honorary Fellow ofthe R...Editor-in-Chief:Xinhe Bao Professor,President of University of Science and Technology of China,Academician of Chinese Academy of Sciences(CAS),Academician of The World Academy of Sciences(TWAS),Honorary Fellow ofthe Royal Society of Chemistry(HonFRSC).展开更多
Single-base editors,including cytosine base editors(CBEs)and adenine base editors(ABEs),facilitate accurate C·G to T·A and A·T to G·C,respectively,holding promise for the precise modeling and treat...Single-base editors,including cytosine base editors(CBEs)and adenine base editors(ABEs),facilitate accurate C·G to T·A and A·T to G·C,respectively,holding promise for the precise modeling and treatment of human hereditary disorders.Efficient base editing and expanded base conversion range have been achieved in human cells through base editors fusing with Rad51 DNA binding domain(Rad51DBD),such as hyA3A-BE4max.Here,we show that hyA3A-BE4max catalyzes C-to-T substitution in the zebrafish genome and extends editing positions(C_(12)-C_(16))proximal to the protospacer adjacent motif.We develop a codon-optimized counterpart zhyA3A-CBE5,which exhibits substantially high C-to-T conversion with 1.59-to 3.50-fold improvement compared with the original hyA3A-BE4max.With these tools,disease-relevant hereditary mutations can be more efficaciously generated in zebrafish.We introduce human genetic mutation rpl11^(Q42*)and abcc6a^(R1463C) by zhyA3A-CBE5 in zebrafish,mirroring Diamond-Blackfan anemia and Pseudoxanthoma Elasticum,respectively.Our study expands the base editing platform targeting the zebrafish genomic landscape and the application of single-base editors for disease modeling and gene function study.展开更多
Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is a monogenic small vessel disease caused by mutations in the NOTCH3 gene. However, the pathogenesis of CADASIL rem...Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is a monogenic small vessel disease caused by mutations in the NOTCH3 gene. However, the pathogenesis of CADASIL remains unclear, and patients have limited treatment options. Here, we use human induced pluripotent stem cells (hiPSCs) generated from the peripheral blood mononuclear cells of a patient with CADASIL carrying a heterozygous NOTCH3 mutation (c.1261C>T, p.R421C) to develop a disease model. The correction efficiency of different adenine base editors (ABEs) is tested using the HEK293T-NOTCH3 reporter cell line. ABEmax is selected based on its higher efficiency and minimization of predicted off-target effects. Vascular smooth muscle cells (VSMCs) differentiated from CADASIL hiPSCs show NOTCH3 deposition and abnormal actin cytoskeleton structure, and the abnormalities are recovered in corrected hiPSC-derived VSMCs. Furthermore, CADASIL blood vessel organoids generated for in vivo modeling show altered expression of genes related to disease phenotypes, including the downregulation of cell adhesion, extracellular matrix organization, and vessel development. The dual adeno-associated virus (AAV) split-ABEmax system is applied to the genome editing of vascular organoids with an average editing efficiency of 8.82%. Collectively, we present potential genetic therapeutic strategies for patients with CADASIL using blood vessel organoids and the dual AAV split-ABEmax system.展开更多
The Editorial Office of Water Science and Engineering would like to express their sincere appreciation to the academic editors including Prof.Carlo Gualtieri from University of Napoli Federico II,Italy,Prof.Guo-qing W...The Editorial Office of Water Science and Engineering would like to express their sincere appreciation to the academic editors including Prof.Carlo Gualtieri from University of Napoli Federico II,Italy,Prof.Guo-qing Wang and Prof.Zhong-zhi Fu from Nanjing Hydraulic Research Institute,China,and Prof.Yan-hui Ao,Prof.Ching-sheng Huang,Prof.Guang-qiu Jin,Prof.Bin Xu,Prof.Sai-yu Yuan,Prof.Zeng Zhou,Prof.Bo Chen,and Prof Da-wei Guan from Hohai Uiversity,China,for their great effort and contribution to WSE in the year 2023.展开更多
The clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein(Cas) system has been widely used for genome editing. In this system, the cytosine base editor(CBE) and adenine base edit...The clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein(Cas) system has been widely used for genome editing. In this system, the cytosine base editor(CBE) and adenine base editor(ABE) allow generating precise and irreversible base mutations in a programmable manner and have been used in many different types of cells and organisms. However, their applications are limited by low editing efficiency at certain genomic target sites or at specific target cytosine(C) or adenine(A) residues. Using a strategy of combining optimized synergistic core components, we developed a new multiplex super-assembled ABE(sABE) in rice that showed higher base-editing efficiency than previously developed ABEs. We also designed a new type of nuclear localization signal(NLS) comprising a FLAG epitope tag with four copies of a codon-optimized NLS(F4NLS^(r2)) to generate another ABE named F4NLS-sABE. This new NLS increased editing efficiency or edited additional A at several target sites. A new multiplex super-assembled CBE(sCBE) and F4NLS^(r2) involved F4NLS-sCBE were also created using the same strategy. F4NLS-sCBE was proven to be much more efficient than sCBE in rice. These optimized base editors will serve as powerful genome-editing tools for basic research or molecular breeding in rice and will provide a reference for the development of superior editing tools for other plants or animals.展开更多
The Editorial Office of Water Science and Engineering would like to express their sincere appreciation to the academic editors including Prof. Carlo Gualtieri from University of Napoli Federico Ⅱ,Italy,Prof.Guo-qing ...The Editorial Office of Water Science and Engineering would like to express their sincere appreciation to the academic editors including Prof. Carlo Gualtieri from University of Napoli Federico Ⅱ,Italy,Prof.Guo-qing Wang and Prof.Zhong-zhi Fu from Nanjing Hydraulic Research Institute,China,and Prof.Yan-hui Ao,Prof.Ching-sheng Huang,Prof.Guang-qiu Jin,Prof.Bin Xu,Prof.Sai-yu Yuan,and Prof.Zeng Zhou from Hohai Uiversity,China,for their great effort and contribution to WSE in the year 2019.展开更多
vip Editor: Yonghui Deng, Department of Chemistry, Fudan University He received his B.S. in chemistry from Nanchang University (2000) and PhD in polymer chemistry and physics from Fudan University (2005). He worked ...vip Editor: Yonghui Deng, Department of Chemistry, Fudan University He received his B.S. in chemistry from Nanchang University (2000) and PhD in polymer chemistry and physics from Fudan University (2005). He worked as a postdoctoral researcher with Prof. Dongyuan Zhao (2005.10-2007.09), and was promoted as associate professor (2007.09) and full professor (2011) in Department of Chemistry at Fudan University. He worked as a visiting scholar with Prof. Peidong Yang in University of California at Berkeley (2009-2010).展开更多
OBJECTIVE:To explore consensus among Traditional Chinese Medicine(TCM)/ integrative medicine(IM) journal executive editors in China regarding:(a)Their perceptions about the differences between papers published in Chin...OBJECTIVE:To explore consensus among Traditional Chinese Medicine(TCM)/ integrative medicine(IM) journal executive editors in China regarding:(a)Their perceptions about the differences between papers published in Chinese/English journals;(b)the key elements required for publishing a good quality paper;(c) how editors manage and decide on the manuscripts submitted to their journals.METHODS:A three classic round Delphi was conducted with a purposive sample of TCIWIM Chinese journal executive editors.The key themes and items generated from six open ended questions in the first round were redistributed to the editors in two subsequent questionnaire rounds and scored on a five-point Likert scale.Consensus,reliability and stability of responses were assessed between the editors in the same round and between rounds.RESULTS:All ten editors initially agreeing to participate completed the three rounds,provided 60 comments in the first round,relating to 6 themes on:elements of publishing good quality article;differences between Chinese and English published articles;reasons for low quality of Chinese studies;maintaining the journal's good reputation;initiatives and strategies;and differences between Chinese and English versions of the same journal.High levels of consensus were reached for 97%items.Intra-class correlation(ICC) coefficient for agreement among editors was high in both second and third round and there was a good stability between the two rounds.CONCLUSION:High inter/intra-editor agreement was identified in the differences between TCM/IM research papers published in English and Chinese.Publishing standards and probity are keys in elevating the reputation of research.Editors' perspectives in maintaining and improving quality journal can be viewed as fundamental for editors and researchers in publishing any articles.展开更多
文摘Quanming Lu,Prosessor,Changjiang Distinguished Professor,National Science Fund for Distinguished Young Scholars.Rongbin Zhou,Prosessor,Changjiang Distinguished Professor,National Science Fund for Distinguished Young Scholars,Deputy Director,Life Sciences and Medicine,USTC.
文摘Han-Ping Shi,M.D.,Ph.D.,FACS,Professor of Surgery,Oncology and Nutrition.He is the current Chairman of the Department of GI Surgery/Clinical Nutrition at Beijing Shijitan Hospital,Capital Medical University,Beijing,China.Dr.Shi received his M.D.degree at the School of Medicine,Wuhan University,Wuhan,and his Ph.D.degree at the Second Military Medical University,Shanghai,China.
文摘The Editorial Office of Water Science and Engineering would like to express their sincere appreciation to the academic editors including Prof.Carlo Gualtieri from University of Napoli Federico Ⅱ,Italy,Prof.Guo-qing Wang and Prof.Zhong-zhi Fu from Nanjing Hydraulic Research Institute,China,and Prof.Yan-hui Ao,Prof.Ching-sheng Huang,Prof.Guang-qiu Jin,Prof.Bin Xu,Prof.Sai-yu Yuan,Prof.Zeng Zhou,Prof.Bo Chen,and Prof.Da-wei Guan from Hohai Uiversity,China,for their great effort and contribution to WSE in the year 2024.
文摘Editor-in-Chief:Xinhe Bao Professor,President of University of Science and Technology of China,Academician of Chinese Academy of Sciences(CAS),Academician of The World Academy of Sciences(TWAS),Honorary Fellow ofthe Royal Society of Chemistry(HonFRSC).
基金supported by grants from Ministry of Science and Technology of the People's Republic of China(2018YFA0801004 and 2018YFA0800103)the National Natural Science Foundation of China(NSFC31530044,NSFC31970780,NSFC82202056).
文摘Single-base editors,including cytosine base editors(CBEs)and adenine base editors(ABEs),facilitate accurate C·G to T·A and A·T to G·C,respectively,holding promise for the precise modeling and treatment of human hereditary disorders.Efficient base editing and expanded base conversion range have been achieved in human cells through base editors fusing with Rad51 DNA binding domain(Rad51DBD),such as hyA3A-BE4max.Here,we show that hyA3A-BE4max catalyzes C-to-T substitution in the zebrafish genome and extends editing positions(C_(12)-C_(16))proximal to the protospacer adjacent motif.We develop a codon-optimized counterpart zhyA3A-CBE5,which exhibits substantially high C-to-T conversion with 1.59-to 3.50-fold improvement compared with the original hyA3A-BE4max.With these tools,disease-relevant hereditary mutations can be more efficaciously generated in zebrafish.We introduce human genetic mutation rpl11^(Q42*)and abcc6a^(R1463C) by zhyA3A-CBE5 in zebrafish,mirroring Diamond-Blackfan anemia and Pseudoxanthoma Elasticum,respectively.Our study expands the base editing platform targeting the zebrafish genomic landscape and the application of single-base editors for disease modeling and gene function study.
基金funded by the National Natural Science Foundation of China(31971365)the Guangdong Basic and Applied Basic Research Foundation(2020B1515120090)the Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program(2019BT02Y276).
文摘Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is a monogenic small vessel disease caused by mutations in the NOTCH3 gene. However, the pathogenesis of CADASIL remains unclear, and patients have limited treatment options. Here, we use human induced pluripotent stem cells (hiPSCs) generated from the peripheral blood mononuclear cells of a patient with CADASIL carrying a heterozygous NOTCH3 mutation (c.1261C>T, p.R421C) to develop a disease model. The correction efficiency of different adenine base editors (ABEs) is tested using the HEK293T-NOTCH3 reporter cell line. ABEmax is selected based on its higher efficiency and minimization of predicted off-target effects. Vascular smooth muscle cells (VSMCs) differentiated from CADASIL hiPSCs show NOTCH3 deposition and abnormal actin cytoskeleton structure, and the abnormalities are recovered in corrected hiPSC-derived VSMCs. Furthermore, CADASIL blood vessel organoids generated for in vivo modeling show altered expression of genes related to disease phenotypes, including the downregulation of cell adhesion, extracellular matrix organization, and vessel development. The dual adeno-associated virus (AAV) split-ABEmax system is applied to the genome editing of vascular organoids with an average editing efficiency of 8.82%. Collectively, we present potential genetic therapeutic strategies for patients with CADASIL using blood vessel organoids and the dual AAV split-ABEmax system.
文摘The Editorial Office of Water Science and Engineering would like to express their sincere appreciation to the academic editors including Prof.Carlo Gualtieri from University of Napoli Federico II,Italy,Prof.Guo-qing Wang and Prof.Zhong-zhi Fu from Nanjing Hydraulic Research Institute,China,and Prof.Yan-hui Ao,Prof.Ching-sheng Huang,Prof.Guang-qiu Jin,Prof.Bin Xu,Prof.Sai-yu Yuan,Prof.Zeng Zhou,Prof.Bo Chen,and Prof Da-wei Guan from Hohai Uiversity,China,for their great effort and contribution to WSE in the year 2023.
基金supported by the Beijing Scholars Program[BSP041]。
文摘The clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein(Cas) system has been widely used for genome editing. In this system, the cytosine base editor(CBE) and adenine base editor(ABE) allow generating precise and irreversible base mutations in a programmable manner and have been used in many different types of cells and organisms. However, their applications are limited by low editing efficiency at certain genomic target sites or at specific target cytosine(C) or adenine(A) residues. Using a strategy of combining optimized synergistic core components, we developed a new multiplex super-assembled ABE(sABE) in rice that showed higher base-editing efficiency than previously developed ABEs. We also designed a new type of nuclear localization signal(NLS) comprising a FLAG epitope tag with four copies of a codon-optimized NLS(F4NLS^(r2)) to generate another ABE named F4NLS-sABE. This new NLS increased editing efficiency or edited additional A at several target sites. A new multiplex super-assembled CBE(sCBE) and F4NLS^(r2) involved F4NLS-sCBE were also created using the same strategy. F4NLS-sCBE was proven to be much more efficient than sCBE in rice. These optimized base editors will serve as powerful genome-editing tools for basic research or molecular breeding in rice and will provide a reference for the development of superior editing tools for other plants or animals.
文摘The Editorial Office of Water Science and Engineering would like to express their sincere appreciation to the academic editors including Prof. Carlo Gualtieri from University of Napoli Federico Ⅱ,Italy,Prof.Guo-qing Wang and Prof.Zhong-zhi Fu from Nanjing Hydraulic Research Institute,China,and Prof.Yan-hui Ao,Prof.Ching-sheng Huang,Prof.Guang-qiu Jin,Prof.Bin Xu,Prof.Sai-yu Yuan,and Prof.Zeng Zhou from Hohai Uiversity,China,for their great effort and contribution to WSE in the year 2019.
文摘vip Editor: Yonghui Deng, Department of Chemistry, Fudan University He received his B.S. in chemistry from Nanchang University (2000) and PhD in polymer chemistry and physics from Fudan University (2005). He worked as a postdoctoral researcher with Prof. Dongyuan Zhao (2005.10-2007.09), and was promoted as associate professor (2007.09) and full professor (2011) in Department of Chemistry at Fudan University. He worked as a visiting scholar with Prof. Peidong Yang in University of California at Berkeley (2009-2010).
文摘OBJECTIVE:To explore consensus among Traditional Chinese Medicine(TCM)/ integrative medicine(IM) journal executive editors in China regarding:(a)Their perceptions about the differences between papers published in Chinese/English journals;(b)the key elements required for publishing a good quality paper;(c) how editors manage and decide on the manuscripts submitted to their journals.METHODS:A three classic round Delphi was conducted with a purposive sample of TCIWIM Chinese journal executive editors.The key themes and items generated from six open ended questions in the first round were redistributed to the editors in two subsequent questionnaire rounds and scored on a five-point Likert scale.Consensus,reliability and stability of responses were assessed between the editors in the same round and between rounds.RESULTS:All ten editors initially agreeing to participate completed the three rounds,provided 60 comments in the first round,relating to 6 themes on:elements of publishing good quality article;differences between Chinese and English published articles;reasons for low quality of Chinese studies;maintaining the journal's good reputation;initiatives and strategies;and differences between Chinese and English versions of the same journal.High levels of consensus were reached for 97%items.Intra-class correlation(ICC) coefficient for agreement among editors was high in both second and third round and there was a good stability between the two rounds.CONCLUSION:High inter/intra-editor agreement was identified in the differences between TCM/IM research papers published in English and Chinese.Publishing standards and probity are keys in elevating the reputation of research.Editors' perspectives in maintaining and improving quality journal can be viewed as fundamental for editors and researchers in publishing any articles.
