Torrentophryne aspinia is a new kind of toads discovered in China recently, it lives in the forest floor in West Yunnan, the most important things for this kind of species is that they have a kind of torrent-adapted t...Torrentophryne aspinia is a new kind of toads discovered in China recently, it lives in the forest floor in West Yunnan, the most important things for this kind of species is that they have a kind of torrent-adapted tadpoles which have abdominal suckers and can adhere to the surfaces of rocks in moving waters. In this article, we described the forming procedures of abdominal sucker and some breeding behaviors, habitats and habits. After comparing with those of other toads, it is confirmed that Torrentophryne should be a new valid genus, it is derived from ancestor toads because of adaptation to the torrent habitats, by the forming of torrent -adapted organ-abdominal sucker in the early development.展开更多
Chondrus is a type of commercially produced red seaweed that widely used for food and carrageen extraction. Although the natural life history of the alga had been well understood, the factors influencing development o...Chondrus is a type of commercially produced red seaweed that widely used for food and carrageen extraction. Although the natural life history of the alga had been well understood, the factors influencing development of the tetraspore and carpospore remain poorly understood. In the perspective of seedling resources, the regulation of early development is crucial for the seedlillg nursing; therefore, it is necessary to understand the physiological influences during its early development. In this study, we studied the effects of temperature and irradiance on the early development of Chondrus ocellatus Holm under laboratory conditions. The released tetraspores and carpospores were cultivated at different temperatures （10-28℃） and irradiances （10, 60 μmol photons m^-2s^-1） with a photoperiod of 12L： 12D. The results indicate that both tetraspores and carpospores are tolerant to temperatures of 10-25℃, and have the highest relative growth rate at 20℃. Irradiance variances influenced the growth of the discoid crusts, and the influence was more significant with increasing temperature; 60 μmol photons m^-2s^-1 was more suitable than 10 μmol photons m^-2s^-1. The optimum temperature and irradiance for the development of seedlings was 20℃ and 60 μmol photons m^-2s^-1, respectively.展开更多
Grateloupia turuturu is a commercial red alga with potential value in nutraceuticals and pharmaceuticals. To supplement information on its life history and verify whether carpospores can be used for seedling culture, ...Grateloupia turuturu is a commercial red alga with potential value in nutraceuticals and pharmaceuticals. To supplement information on its life history and verify whether carpospores can be used for seedling culture, early development of G. turuturu was investigated under culture conditions （27~C, 10-13 ~mol/（mZ.s） in irradiance, photoperiod 10：14 h L：D）. Three physiological stages were recognized by continuous microscopic observation： division stage, discoid crust stage, and juvenile seedling stage. At the beginning of the division stage, the carpospores developed germ tubes into which the carpospore protoplasm was evacuated, and then the carpospore protoplasm in the germ tubes began to divide continuously until discoid crusts formed. Finally, upright thalli appeared on the discoid crusts and developed into juvenile seedlings. It took about 60 days for carpospores to develop into juvenile seedlings. The growth parameters, including germination rate for carpospores and discoid crust diameter, were recorded. These results contribute more information on the life cycle, and at the same time are of great significance in the scaling-up of artificial seedling cultures of G. turuturu.展开更多
In this study, we evaluated the effects of CO_2-induced seawater acidification on fertilization, embryogenesis and early larval development in the sea urchin Glyptocidaris crenularis, that inhabits subtidal coastal ar...In this study, we evaluated the effects of CO_2-induced seawater acidification on fertilization, embryogenesis and early larval development in the sea urchin Glyptocidaris crenularis, that inhabits subtidal coastal areas in northern China. The range in seawater p H used in experiments was based on the projections of the Intergovernmental Panel on Climate Change(IPCC), to the year 2100. A natural seawater treatment(p H_(nbs) =7.98±0.03) and three laboratory-controlled acidified treatments(OA_1, ΔpH_(nbs) =-0.3 units; OA_2, ΔpH_(nbs) =-0.4 units; OA_3, ΔpH_(nbs) =-0.5 units) were used in experiments. Results show that:(1) there was a negative effect of seawater acidification on fertilization and on the percentage of abnormal fertilized eggs;(2) the size of early cleavage stage embryos decreased in a dose-dependent manner with decreasing p H;(3) both the hatching rate of blastulae and the survival rate of four-armed pluteus larvae decreased as pH declined;(4) larval abnormalities including asymmetrical development, changes in the length of skeletal elements, and corroded spicules were observed in all seawater acidified-treatments compared with the control. These data indicate that seawater acidification has a negative impact on the early development of G. crenularis, and supports the hypothesis that the response of echinoderms to ocean acidification(OA) varies among species. Further research is required to clarify the specific cellular mechanisms involved.展开更多
Differential expression of genes is crucial to growth and development of fish. To select the appropriate genes for gene normalization during Cynoglossus semilaevis early developmental process, eight candidate referenc...Differential expression of genes is crucial to growth and development of fish. To select the appropriate genes for gene normalization during Cynoglossus semilaevis early developmental process, eight candidate reference genes （ACTB, B2M, EF1A, GADPH, RPL7, TUBA, UBCE and 18S） were tested for their adequacy by using quantitative real-time PCR. The results showed that the expression of all the examined genes exhibited tissue dependent variations in the mature C. semilaevis. EFIA was listed as the most stable reference among the 14 tissues by RefFinder. Furthermore, the recommended comprehensive ranking of the stability determined by RefFinder showed that 18S was the most stable gene during the early developmental stages （from oosphere to 90 days old） in this study. However, when divided the Ct value data of the above mentioned early developmental stages into two separate periods （embryo and post-hatching periods）, TUBA and 18S represented the most stable references of these two developmental periods, respectively. Consequently, the reference gene should be carefully and accurately chosen even for studies of the same species at various developmental processes. The relevant data may help in selecting appropriate reference genes for mRNA expression analysis, and is of great value in the studies of fish growth and development.展开更多
Chondrus is an economically important red algae widely used for food and biochemical purpose.It early development is crucial for the culture and seedling propagation.We chose tetraspores and carpospores of Chondrus oc...Chondrus is an economically important red algae widely used for food and biochemical purpose.It early development is crucial for the culture and seedling propagation.We chose tetraspores and carpospores of Chondrus ocellatus as examples for experiment of the culture,induction and release in laboratory condition,aiming to understand early development of C.ocellatus and to apply in seedling production.Mature C.ocellatus were collected in Qingdao,China,from Nov.to Dec.2004.After the gametophyte and tetrasporophyte were brushed and washed with sterilized seawater,the algal materials were treated in 1.5%KI for 20 min,then were dried for lh to stimulate the releasing of spores.After the spores released overnight,it were cultured in PES medium,incubated at 18℃,10±2μmol/(m^(2).s^(1))in 12:12h(light:dark).The observation and recording under microscope were carried out.Continuous observation of the early development showed that both tetraspore and carpospore are similar to each other.In general,three stages of the early development were shown being division,discoid crust and seedling stages.To the division stage,the most obvious feature was the increasing of cell number;during the discoid crust stage,the discoid crust had a three-dimensional axis,and it began to differentiate into two types of cells:the basal cells and the apical cells;and to the seedling stage,several protuberances-like appeared on the discoid crusts and formed juvenile seedlings.Carpospores and tetraspores exhibited a similar development process that included division stage,discoid crust stage and seedling stage.展开更多
Study on the early development of sporelings from carpospores of Gracilaria asiatica Chang et Xia was conducted indoors under controlled culture conditions. Besides normal development of sporelings, a new developmenta...Study on the early development of sporelings from carpospores of Gracilaria asiatica Chang et Xia was conducted indoors under controlled culture conditions. Besides normal development of sporelings, a new developmental phenomenon of filamentous frond was observed. It was composed of one or two rows of cells, and took place from the outmost brim of the basal disc. During the early disc stage of germinated carpospores, one or two filamentous fronds formed on about 10% basal discs. Simul-taneously, young fronds began to arch slightly from the centers of single and coalescent discs; lately more filamentous fronds up to 80% appeared on the brims of basal discs. Meanwhile one or more upright fronds protuberated on the basal discs. Generally, filamentous fronds exhibited in self-existence or co-existence forms with normal young sporelings on the same basal disc, and single cell detached from filamentous fronds developed into a new filamentous frond. This new phenomenon exhibited a unique differentiation pathway during the early development of G. asiatica, which would be potential for the ap-plication in artificial sporelings nursery.展开更多
Polysiphonia urceolata is one type of potential commercial red seaweeds used for breeding and cultivation, because of its significant biochemical and biomedical application. However, the information of breeding and se...Polysiphonia urceolata is one type of potential commercial red seaweeds used for breeding and cultivation, because of its significant biochemical and biomedical application. However, the information of breeding and seedling incubation for cultivation is limited, especially the early development. In this study, tetrasporohyte and gametophyte of P. urceolata were taken as the study materials in Huiquan Bay, Qingdao, China. The cleaned and sterilized tetrasporophytes and gametophytes were pre-cultured in sterilized seawater, then nurtured at 18℃, 25 μmol photons m^-2 s^-1 in 12：12 h （light：dark） photoperiod. Continuous observation under microscope showed that the early development consists of bipolar division stage and seedling stage. In the division stage, tetraspores germinate into bipolar sporelings that further differentiate into a colorless rhizoidal portion and a lightly pigmented upright shoot. The lightly pigmented rhizoidal cell develops to a rhizoid and the larger pigmented cell transforms to an erect axis. In the seedling stage, several quasi-protuberances appear on the erect axis and form juvenile seedlings. The results demonstrate the culture of P. urceolata from tetraspores under laboratory conditions.展开更多
We studied early development of peer dominance relationships in a captive group of Japanese macaques Macaca fuscata fuscata at the Primate Research Institute of Kyoto University. This study aims to give detailed descr...We studied early development of peer dominance relationships in a captive group of Japanese macaques Macaca fuscata fuscata at the Primate Research Institute of Kyoto University. This study aims to give detailed descriptions on characteristic patterns of maternal rank acquisition from infant to juvenile. Focal subjects were 22 young monkeys belonging to three cohorts born in 2002, 2003 and 2005. Data were collected with a total 2130 sessions of 30-minute continuous recording of focal subjects combined with all occurrence-sampling methods. The onset of aggressive behavior varied per cohort and was delayed in cohorts with fewer close-aged associates. More than 60% of dyadic combinations in agonistic interactions between peers were unidirectional throughout the study period. Although some bidirectional interactions could have involved unstable relationships between particular individuals, most of the bidirectional interactions included a few continuous series of alternating one-sided interactions. A linear order could be found among peers from the first appearance of aggressive behavior, and nearly 90% of those dyads were concordant with that of their mother＇s rank order. Young males were responsible for most of the dominance relations that would not be predicted based on their mother＇s rank. These results suggest that infant monkeys may recognize their own social status relative to their opponent＇s before onset of aggressive behavior and adjust themselves into the matrilineal rank system accordingly.展开更多
Silvetia babingtonii is a potentially economic brown alga for sources of food and high-value added utilization. So far, sporeling nursery and field cultivation has not been successful. The lack of knowledge on develop...Silvetia babingtonii is a potentially economic brown alga for sources of food and high-value added utilization. So far, sporeling nursery and field cultivation has not been successful. The lack of knowledge on development and life cycle of this alga hinder the development of techniques for the sporeings and cultivation. In this study, internal structure of oogonium and antherium of S. babingtonii was observed with hematoxylin and eosin staining and through microscope. Meanwhile, early development from zygotes to juvenile sporelings was studied at 20℃ under 60–100 μmol photons m-2 s-1. Zygotes germinated and divided into thallus and rhizoid cells. The larger thallus cells further divided and developed into juvenile sporelings; while the smaller rhizoid cells divided and elongated into rhizoid hairs. These findings documented the life cycle of S. babingtonii and provided fundamental knowledge for sporeling nursery in the near future.展开更多
Ejaculated mammalian spermatozoa contain a complex yet specific population of mRNA. However, the possible roles that mRNA has in early zygotic and embryonic development remain unclear. We found that Dby mRNA is select...Ejaculated mammalian spermatozoa contain a complex yet specific population of mRNA. However, the possible roles that mRNA has in early zygotic and embryonic development remain unclear. We found that Dby mRNA is selectively retained in capacitated mouse spermatozoa, and is transferred into the oocyte during fertilization by reverse transcription-polymerase chain reaction even though no DBY protein expression is detected. The cellular location ofDby mRNA is seen in the post-acrosome region, and it comprises nearly half of the mouse spermatozoa in in situ hybridization. In contrast, transcripts of the control gene, Smcy, are not detected in capacitated mouse spermatozoa, although the H-Y antigen encoded by Smcy is expressed on the surface of the spermatozoa. In our microinjection experiment, the zygotic development rate of the as-Dby male pronucleus injection group was significantly lower than that of the as-Smcy male pronucleus injection group （35.9% vs. 95%, P = 0.001） and the as-Dby female pronucleus injection group （35.9% vs. 93.8%, P = 0.001）. The rate of male-developed zygotes was also lower than that of the as-Smcy male pronucleus injection group （17.4% vs. 57.9%, P = 0.002） and the as-Dby female pronucleus injection group （17.4% vs. 54.1%, P = 0.002）. Thus, we conclude that Dby mRNA is selectively retained in capaci- tated mouse spermatozoa, and it has an important role in the early zygotic development of male mouse zygotes. This might imply that spermatozoa mRNA is involved in early zygotic and embryonic stages of reproduction.展开更多
The early developmental period is a critical window during which brain cells mature and contribute to both brain development and later life functions.Gamma-aminobutyric acid(GABA),recognized as a major neurotransmitte...The early developmental period is a critical window during which brain cells mature and contribute to both brain development and later life functions.Gamma-aminobutyric acid(GABA),recognized as a major neurotransmitter,plays a crucial role in coordinating synapse formation,neuronal proliferation,and migration during this time.展开更多
More and more studies have demonstrated that pseudogenes possess coding ability,and the functions of their transcripts in the development of diseases have been partially revealed.However,the role of pseudogenes in mai...More and more studies have demonstrated that pseudogenes possess coding ability,and the functions of their transcripts in the development of diseases have been partially revealed.However,the role of pseudogenes in maintenance of normal physiological states and life activities has long been neglected.Here,we identify pseudogenes that are dynamically expressed during human early embryogenesis,showing different expression patterns from that of adult tissues.We explore the expression correlation between pseudogenes and the parent genes,partly due to their shared gene regulatory elements or the potential regulation network between them.The essential role of three pseudogenes,PI4KAP1,TMED10P1,and FBXW4P1,in maintaining self-renewal of human embryonic stem cells is demonstrated.We further find that the three pseudogenes might perform their regulatory functions by binding to proteins or microRNAs.The pseudogene-related single-nucleotide polymorphisms are significantly associated with human congenital disease,further illustrating their importance during early embryonic development.Overall,this study is an excavation and exploration of functional pseudogenes during early human embryonic development,suggesting that pseudogenes are not only capable of being specifically activated in pathological states,but also play crucial roles in the maintenance of normal physiological states.展开更多
Introduction:Early childhood development(ECD)centres are important community hubs in South Africa and act as sites for community detection of childhood nutrition problems.This study aimed to assess the ability of trai...Introduction:Early childhood development(ECD)centres are important community hubs in South Africa and act as sites for community detection of childhood nutrition problems.This study aimed to assess the ability of trained ECD practitioners with optimal support to correctly classify the nutritional status of infants and young children at ECD centres in the Nelson Mandela Bay.Methods:A descriptive,cross-sectional study was used to collect data from 1645 infants and children at 88 ECD centres.Anthropometric measurements were taken by trained fieldworkers and growth monitoring and promotion infrastructure was audited at ECD centres.Results:Of the sample,4.4%(n=72)were underweight by weight for age Z-score(WAZ<-2)and 0.8%(n=13)were severely underweight(WAZ<-3).Results showed that 13.1%(n=214)were stunted by height for age Z-score(HAZ<-2)and 4.5%(n=74)were severely stunted(HAZ<-3).The prevalence of moderate acute malnutrition was 1.2%and severe acute malnutrition was 0.5%,while the prevalence of overweight was 9.2%and the prevalence of obesity was 4%.A significant level of agreement between the correct interpretation and the ECD practitioners'interpretation was observed across all the anthropometric indicators investigated.The true positive wasting cases had a mean mid-upper arm circumference(MUAC)of 14.6 cm,which may explain the high false negative rate found in terms of children identified with wasting,where ECD practitioners fail to use the weight for height Z-score(WHZ)interpretation for screening.Conclusion:By using ECD centres as hub to screen for malnutrition,it may contribute to the early identification of failure to thrive among young children.Although it was concerning that trained ECD practitioners are missing some children with an unacceptably high false negative rate,it may have been due to the fact that wasting in older children cannot be identified with MUAC alone and that accurate WFH plotting is needed.Onsite mentorship by governmental health workers may provide ECD practitioners with more confidence to screen children for growth failure based on regular WFH measurements.Moreover,ECD practitioners will be more confident to monitor the Road to Health booklets for missed vaccinations,vitamin A and deworming opportunities.展开更多
Cell polarization and asymmetric cell divisions play important roles during development in many multicellular eukaryotes. Fucoid algae have a long history as models for studying early developmental processes, probably...Cell polarization and asymmetric cell divisions play important roles during development in many multicellular eukaryotes. Fucoid algae have a long history as models for studying early developmental processes, probably because of the ease with which zygotes can be observed and manipulated in the laboratory. This review discusses cell polarization and asymmetric cell divisions in fucoid algal zygotes with an emphasis on the roles played by the cytoskeleton.展开更多
Objective: to explore the effect of early intervention nursing measures to promote the development of infant growth and development in children. Methods: in order to comprehensively improve the early growth and develo...Objective: to explore the effect of early intervention nursing measures to promote the development of infant growth and development in children. Methods: in order to comprehensively improve the early growth and development of children and implement the intervention nursing measures, 80 cases of infants who visited our department from July 2019 to July 2020 were selected and divided into the control group and the research group. The control group received routine early childhood care. On the basis of the control group, the research group adopted the child health care intervention nursing measures to give full play to the influence of the two groups in caring for the growth and development of children. Results: effective analysis of the physical conditions of infants in different groups temporarily after the 12-month intervention nursing intervention showed that their average height was (76.85±6.27)cm, and the data from the comprehensive analysis study group were significantly superior to those of the control group (73.02±1.93)cm. The data on the related clinical nursing intervention effects of infants in the two groups were statistically significant (P < 0.05). In terms of NET score, by exploring the growth and development of infants in two groups, the score data of the research group at 6 months, 9 months and 12 months were significantly better than the application related data of the control group. The difference between the two groups was statistically significant (P < 0.05). Conclusion: the effective combination of early implementation of scientific management interventions in two groups of infants significantly promotes the growth and development of children in all aspects. Through comparative analysis, the growth and development as well as the intelligence development of infants measured by the growth and development status of infants in the research group are significantly better than those in the control group, which is worthy of clinical promotion.展开更多
[ Objective] To study the growth-development of Boer goats from embryo transplant. [ Method] With Boer goats from embryo transplant as the tested animals and natural breeding Boer goats born in the same period as CK, ...[ Objective] To study the growth-development of Boer goats from embryo transplant. [ Method] With Boer goats from embryo transplant as the tested animals and natural breeding Boer goats born in the same period as CK, the weight of Boer goats at birth and at the age of 1,3 and 6 months as well as the body size at the age of 3 and 6 months were investigated in this study, while their health conditions were also observed and recorded. [ Result] Under the normal feeding conditions, Boer goats from embryo transplant developed well at early stage, and their meat-using body type were also obvious. The average birth weight of bucks was （4.25±0.95） kg, and that of does was （3.74±0.10） kg. The average weight of bucks at the age of 6 months was （31.90 ±0.74） kg and that of was （25.90 ±0.67） kg. The daily gains of bucks and ewes from birth to 6 months of age were 153.64 and 123.11 g respectively, while their body length, body height and chest circumference increased obviously, and there was no significant difference in body weight or body size between them and natural breeding goats. [ Condusioa] The study lays a foundation for further studies on the breedinq and utilization of Boer noats from embryo transplant at early staae.展开更多
RanBP1 is a binding protein of Ran that plays a pivotal role in nucleocytoplasmic transport.In this study,the localization and possible functions of RanBP1 were examined,during the early embryonic development of mice....RanBP1 is a binding protein of Ran that plays a pivotal role in nucleocytoplasmic transport.In this study,the localization and possible functions of RanBP1 were examined,during the early embryonic development of mice.Immunofluorescence results showed that RanBP1 was mainly localized in cytoplasm at mitosis interphase,and its concentration was lower in nucleus and the lowest in nucleolus.With the formation of the spindle in the early embryonic cells,RanBP1 condensed area took the shape of spindle microtubule,the concentration of RanBP1 was low in the site of chromosome.During the formation of nucleus,RanBP1 concentrated in nucleus and there were few dots of RanBP1 around the nucleolus.These dots were lost after the nucleus full growth.The results showed that RanBP1 had important roles in nucleocytoplasmic transport,spindle formation and nuclear assembly in the early embryonic development of mice.展开更多
The paper sought to explore issues of access, equity, and quality in the early childhood development （ECD） sub-sector in Zimbabwe. The massive expansion of ECD services prompted the author to undertake the study. Da...The paper sought to explore issues of access, equity, and quality in the early childhood development （ECD） sub-sector in Zimbabwe. The massive expansion of ECD services prompted the author to undertake the study. Data were collected using focus group discussions, individual interviews, document analysis, and observations. Information from literature, research reports, policies, and practices was also analyzed in order to establish the state of affairs in the provision of ECD. The data were analyzed using theme identification methods to solicit emerging issues. Generally, it was found that on issues of access, equity, and quality in ECD in Zimbabwe, there is a quality-quantity dilemma with qualitative progress less conspicuous than quantitative progress. The resultant specific challenges include： large teacher-child ratio; lack of parental involvement, lack of trained ECD specialists with expertise in using a play-based curriculum; and shortage of equipment, material resources, and age appropriate infrastructure. In most ECD settings, there were inadequate health and nutrition provisions. Water, sanitation and health （WASH） facilities were found to be insufficient in most centres. The study recommended that there is need for： equal access to quality early childhood education; provision of infrastructure, provision of ability and age appropriate resources; and health, sanitation, and nutrition services especially in rural settings. Overall, ECD programmes in Zimbabwe should strive to attain quality more than quantity services. The need to train more early childhood specialists who would drive the ECD sub-sector is emphasized.展开更多
文摘Torrentophryne aspinia is a new kind of toads discovered in China recently, it lives in the forest floor in West Yunnan, the most important things for this kind of species is that they have a kind of torrent-adapted tadpoles which have abdominal suckers and can adhere to the surfaces of rocks in moving waters. In this article, we described the forming procedures of abdominal sucker and some breeding behaviors, habitats and habits. After comparing with those of other toads, it is confirmed that Torrentophryne should be a new valid genus, it is derived from ancestor toads because of adaptation to the torrent habitats, by the forming of torrent -adapted organ-abdominal sucker in the early development.
基金Supported by the Shandong Agriculture Seedstocks Project and the Knowledge Innovation Program of the Chinese Academy of Sciences (No. KSCX2-YW-N-47-02)the National High Techmology Research and Development Program of China (863 Program) (No.2006AA09Z421)and Experimental Marine Biology Lab, IOCAS
文摘Chondrus is a type of commercially produced red seaweed that widely used for food and carrageen extraction. Although the natural life history of the alga had been well understood, the factors influencing development of the tetraspore and carpospore remain poorly understood. In the perspective of seedling resources, the regulation of early development is crucial for the seedlillg nursing; therefore, it is necessary to understand the physiological influences during its early development. In this study, we studied the effects of temperature and irradiance on the early development of Chondrus ocellatus Holm under laboratory conditions. The released tetraspores and carpospores were cultivated at different temperatures （10-28℃） and irradiances （10, 60 μmol photons m^-2s^-1） with a photoperiod of 12L： 12D. The results indicate that both tetraspores and carpospores are tolerant to temperatures of 10-25℃, and have the highest relative growth rate at 20℃. Irradiance variances influenced the growth of the discoid crusts, and the influence was more significant with increasing temperature; 60 μmol photons m^-2s^-1 was more suitable than 10 μmol photons m^-2s^-1. The optimum temperature and irradiance for the development of seedlings was 20℃ and 60 μmol photons m^-2s^-1, respectively.
基金Supported by the Natural Science Foundation of Shandong Province,China(No.Y2007D42)
文摘Grateloupia turuturu is a commercial red alga with potential value in nutraceuticals and pharmaceuticals. To supplement information on its life history and verify whether carpospores can be used for seedling culture, early development of G. turuturu was investigated under culture conditions （27~C, 10-13 ~mol/（mZ.s） in irradiance, photoperiod 10：14 h L：D）. Three physiological stages were recognized by continuous microscopic observation： division stage, discoid crust stage, and juvenile seedling stage. At the beginning of the division stage, the carpospores developed germ tubes into which the carpospore protoplasm was evacuated, and then the carpospore protoplasm in the germ tubes began to divide continuously until discoid crusts formed. Finally, upright thalli appeared on the discoid crusts and developed into juvenile seedlings. It took about 60 days for carpospores to develop into juvenile seedlings. The growth parameters, including germination rate for carpospores and discoid crust diameter, were recorded. These results contribute more information on the life cycle, and at the same time are of great significance in the scaling-up of artificial seedling cultures of G. turuturu.
基金Supported by the National Natural Science Foundation of China(No.41206128)the Program for Liaoning Excellent Talents in University(No.LJQ2013079)the National High Technology Research and Development Program of China(863 Program)(No.2012AA10A412)
文摘In this study, we evaluated the effects of CO_2-induced seawater acidification on fertilization, embryogenesis and early larval development in the sea urchin Glyptocidaris crenularis, that inhabits subtidal coastal areas in northern China. The range in seawater p H used in experiments was based on the projections of the Intergovernmental Panel on Climate Change(IPCC), to the year 2100. A natural seawater treatment(p H_(nbs) =7.98±0.03) and three laboratory-controlled acidified treatments(OA_1, ΔpH_(nbs) =-0.3 units; OA_2, ΔpH_(nbs) =-0.4 units; OA_3, ΔpH_(nbs) =-0.5 units) were used in experiments. Results show that:(1) there was a negative effect of seawater acidification on fertilization and on the percentage of abnormal fertilized eggs;(2) the size of early cleavage stage embryos decreased in a dose-dependent manner with decreasing p H;(3) both the hatching rate of blastulae and the survival rate of four-armed pluteus larvae decreased as pH declined;(4) larval abnormalities including asymmetrical development, changes in the length of skeletal elements, and corroded spicules were observed in all seawater acidified-treatments compared with the control. These data indicate that seawater acidification has a negative impact on the early development of G. crenularis, and supports the hypothesis that the response of echinoderms to ocean acidification(OA) varies among species. Further research is required to clarify the specific cellular mechanisms involved.
基金The National Natural Science Foundation of China under contract No.31201981China Postdoctoral Science Foundation under contract No.2013M531658the Special Scientific Research Funds for Central Non-profit Institutes,Yellow Sea Fisheries Research Institutes under contract No.20603022012032
文摘Differential expression of genes is crucial to growth and development of fish. To select the appropriate genes for gene normalization during Cynoglossus semilaevis early developmental process, eight candidate reference genes （ACTB, B2M, EF1A, GADPH, RPL7, TUBA, UBCE and 18S） were tested for their adequacy by using quantitative real-time PCR. The results showed that the expression of all the examined genes exhibited tissue dependent variations in the mature C. semilaevis. EFIA was listed as the most stable reference among the 14 tissues by RefFinder. Furthermore, the recommended comprehensive ranking of the stability determined by RefFinder showed that 18S was the most stable gene during the early developmental stages （from oosphere to 90 days old） in this study. However, when divided the Ct value data of the above mentioned early developmental stages into two separate periods （embryo and post-hatching periods）, TUBA and 18S represented the most stable references of these two developmental periods, respectively. Consequently, the reference gene should be carefully and accurately chosen even for studies of the same species at various developmental processes. The relevant data may help in selecting appropriate reference genes for mRNA expression analysis, and is of great value in the studies of fish growth and development.
基金Supported by NSFC(No.40376049)Shandong Agricultural Seed Stock Breeding Project.
文摘Chondrus is an economically important red algae widely used for food and biochemical purpose.It early development is crucial for the culture and seedling propagation.We chose tetraspores and carpospores of Chondrus ocellatus as examples for experiment of the culture,induction and release in laboratory condition,aiming to understand early development of C.ocellatus and to apply in seedling production.Mature C.ocellatus were collected in Qingdao,China,from Nov.to Dec.2004.After the gametophyte and tetrasporophyte were brushed and washed with sterilized seawater,the algal materials were treated in 1.5%KI for 20 min,then were dried for lh to stimulate the releasing of spores.After the spores released overnight,it were cultured in PES medium,incubated at 18℃,10±2μmol/(m^(2).s^(1))in 12:12h(light:dark).The observation and recording under microscope were carried out.Continuous observation of the early development showed that both tetraspore and carpospore are similar to each other.In general,three stages of the early development were shown being division,discoid crust and seedling stages.To the division stage,the most obvious feature was the increasing of cell number;during the discoid crust stage,the discoid crust had a three-dimensional axis,and it began to differentiate into two types of cells:the basal cells and the apical cells;and to the seedling stage,several protuberances-like appeared on the discoid crusts and formed juvenile seedlings.Carpospores and tetraspores exhibited a similar development process that included division stage,discoid crust stage and seedling stage.
基金Supported by NSFC (No. 40376049) and the Knowledge Innovation Program of Chinese Academy of Sciences and the Shandong Agricultural Seed Stock Breeding Project (Granted to D. L. Duan).
文摘Study on the early development of sporelings from carpospores of Gracilaria asiatica Chang et Xia was conducted indoors under controlled culture conditions. Besides normal development of sporelings, a new developmental phenomenon of filamentous frond was observed. It was composed of one or two rows of cells, and took place from the outmost brim of the basal disc. During the early disc stage of germinated carpospores, one or two filamentous fronds formed on about 10% basal discs. Simul-taneously, young fronds began to arch slightly from the centers of single and coalescent discs; lately more filamentous fronds up to 80% appeared on the brims of basal discs. Meanwhile one or more upright fronds protuberated on the basal discs. Generally, filamentous fronds exhibited in self-existence or co-existence forms with normal young sporelings on the same basal disc, and single cell detached from filamentous fronds developed into a new filamentous frond. This new phenomenon exhibited a unique differentiation pathway during the early development of G. asiatica, which would be potential for the ap-plication in artificial sporelings nursery.
基金Supported by National Key Technology Support Program, Development Program of China (No.2006AA09Z21)National Natural Science Foundation of China (No. 40618001 and N_CUHK438/06)Shandong Agricultural Seed Stock Breeding Project
文摘Polysiphonia urceolata is one type of potential commercial red seaweeds used for breeding and cultivation, because of its significant biochemical and biomedical application. However, the information of breeding and seedling incubation for cultivation is limited, especially the early development. In this study, tetrasporohyte and gametophyte of P. urceolata were taken as the study materials in Huiquan Bay, Qingdao, China. The cleaned and sterilized tetrasporophytes and gametophytes were pre-cultured in sterilized seawater, then nurtured at 18℃, 25 μmol photons m^-2 s^-1 in 12：12 h （light：dark） photoperiod. Continuous observation under microscope showed that the early development consists of bipolar division stage and seedling stage. In the division stage, tetraspores germinate into bipolar sporelings that further differentiate into a colorless rhizoidal portion and a lightly pigmented upright shoot. The lightly pigmented rhizoidal cell develops to a rhizoid and the larger pigmented cell transforms to an erect axis. In the seedling stage, several quasi-protuberances appear on the erect axis and form juvenile seedlings. The results demonstrate the culture of P. urceolata from tetraspores under laboratory conditions.
文摘We studied early development of peer dominance relationships in a captive group of Japanese macaques Macaca fuscata fuscata at the Primate Research Institute of Kyoto University. This study aims to give detailed descriptions on characteristic patterns of maternal rank acquisition from infant to juvenile. Focal subjects were 22 young monkeys belonging to three cohorts born in 2002, 2003 and 2005. Data were collected with a total 2130 sessions of 30-minute continuous recording of focal subjects combined with all occurrence-sampling methods. The onset of aggressive behavior varied per cohort and was delayed in cohorts with fewer close-aged associates. More than 60% of dyadic combinations in agonistic interactions between peers were unidirectional throughout the study period. Although some bidirectional interactions could have involved unstable relationships between particular individuals, most of the bidirectional interactions included a few continuous series of alternating one-sided interactions. A linear order could be found among peers from the first appearance of aggressive behavior, and nearly 90% of those dyads were concordant with that of their mother＇s rank order. Young males were responsible for most of the dominance relations that would not be predicted based on their mother＇s rank. These results suggest that infant monkeys may recognize their own social status relative to their opponent＇s before onset of aggressive behavior and adjust themselves into the matrilineal rank system accordingly.
基金sponsored by the Shandong Agricultural Seedstock Breeding Projectthe Scientific Research Foundation for the Returned Overseas Chinese Scholars, Ministry of Education of China (No. 2010-1561)
文摘Silvetia babingtonii is a potentially economic brown alga for sources of food and high-value added utilization. So far, sporeling nursery and field cultivation has not been successful. The lack of knowledge on development and life cycle of this alga hinder the development of techniques for the sporeings and cultivation. In this study, internal structure of oogonium and antherium of S. babingtonii was observed with hematoxylin and eosin staining and through microscope. Meanwhile, early development from zygotes to juvenile sporelings was studied at 20℃ under 60–100 μmol photons m-2 s-1. Zygotes germinated and divided into thallus and rhizoid cells. The larger thallus cells further divided and developed into juvenile sporelings; while the smaller rhizoid cells divided and elongated into rhizoid hairs. These findings documented the life cycle of S. babingtonii and provided fundamental knowledge for sporeling nursery in the near future.
基金This work is supported by the Agricultural Key Foundation of Shanghai, China （No. 2006-5-6）, the National Natural Science Foundation of China （No. 3091490）, the National Basic Research Program, China （No. 2009CB941704）, PhD Programs Foundation of Ministry of Education of China （No. 200802480026） and the Shanghai Leading Academic Discipline Project, China （No. B205）. We would like to extend our appreciation to Prof. Yi-Tao Zeng of the Shanghai Institute of Medical Genetics and Professor Lin He of the Bio-X Center of the Shanghai Jiao Tong University for their insightful comments.
文摘Ejaculated mammalian spermatozoa contain a complex yet specific population of mRNA. However, the possible roles that mRNA has in early zygotic and embryonic development remain unclear. We found that Dby mRNA is selectively retained in capacitated mouse spermatozoa, and is transferred into the oocyte during fertilization by reverse transcription-polymerase chain reaction even though no DBY protein expression is detected. The cellular location ofDby mRNA is seen in the post-acrosome region, and it comprises nearly half of the mouse spermatozoa in in situ hybridization. In contrast, transcripts of the control gene, Smcy, are not detected in capacitated mouse spermatozoa, although the H-Y antigen encoded by Smcy is expressed on the surface of the spermatozoa. In our microinjection experiment, the zygotic development rate of the as-Dby male pronucleus injection group was significantly lower than that of the as-Smcy male pronucleus injection group （35.9% vs. 95%, P = 0.001） and the as-Dby female pronucleus injection group （35.9% vs. 93.8%, P = 0.001）. The rate of male-developed zygotes was also lower than that of the as-Smcy male pronucleus injection group （17.4% vs. 57.9%, P = 0.002） and the as-Dby female pronucleus injection group （17.4% vs. 54.1%, P = 0.002）. Thus, we conclude that Dby mRNA is selectively retained in capaci- tated mouse spermatozoa, and it has an important role in the early zygotic development of male mouse zygotes. This might imply that spermatozoa mRNA is involved in early zygotic and embryonic stages of reproduction.
基金supported by the Center for Cognition and Sociality,Institute for Basic Science(IBS)(IBS-R001-D2)(to WK).
文摘The early developmental period is a critical window during which brain cells mature and contribute to both brain development and later life functions.Gamma-aminobutyric acid(GABA),recognized as a major neurotransmitter,plays a crucial role in coordinating synapse formation,neuronal proliferation,and migration during this time.
基金supported by the National Key Research and Development Program of China(2021YFA0805703,2019YFA0801800,and 2019YFA0802600)the National Natural Science Foundation of China(82330007,82122005,92268205 and 81970101)+1 种基金CAMS Innovation Fund for Medical Sciences(2021-I2M1-019)Haihe Laboratory of Cell Ecosystem Innovation Fund(22HHXBSS00027)。
文摘More and more studies have demonstrated that pseudogenes possess coding ability,and the functions of their transcripts in the development of diseases have been partially revealed.However,the role of pseudogenes in maintenance of normal physiological states and life activities has long been neglected.Here,we identify pseudogenes that are dynamically expressed during human early embryogenesis,showing different expression patterns from that of adult tissues.We explore the expression correlation between pseudogenes and the parent genes,partly due to their shared gene regulatory elements or the potential regulation network between them.The essential role of three pseudogenes,PI4KAP1,TMED10P1,and FBXW4P1,in maintaining self-renewal of human embryonic stem cells is demonstrated.We further find that the three pseudogenes might perform their regulatory functions by binding to proteins or microRNAs.The pseudogene-related single-nucleotide polymorphisms are significantly associated with human congenital disease,further illustrating their importance during early embryonic development.Overall,this study is an excavation and exploration of functional pseudogenes during early human embryonic development,suggesting that pseudogenes are not only capable of being specifically activated in pathological states,but also play crucial roles in the maintenance of normal physiological states.
基金Nelson Mandela University,London Metropolitan University and UNICEF。
文摘Introduction:Early childhood development(ECD)centres are important community hubs in South Africa and act as sites for community detection of childhood nutrition problems.This study aimed to assess the ability of trained ECD practitioners with optimal support to correctly classify the nutritional status of infants and young children at ECD centres in the Nelson Mandela Bay.Methods:A descriptive,cross-sectional study was used to collect data from 1645 infants and children at 88 ECD centres.Anthropometric measurements were taken by trained fieldworkers and growth monitoring and promotion infrastructure was audited at ECD centres.Results:Of the sample,4.4%(n=72)were underweight by weight for age Z-score(WAZ<-2)and 0.8%(n=13)were severely underweight(WAZ<-3).Results showed that 13.1%(n=214)were stunted by height for age Z-score(HAZ<-2)and 4.5%(n=74)were severely stunted(HAZ<-3).The prevalence of moderate acute malnutrition was 1.2%and severe acute malnutrition was 0.5%,while the prevalence of overweight was 9.2%and the prevalence of obesity was 4%.A significant level of agreement between the correct interpretation and the ECD practitioners'interpretation was observed across all the anthropometric indicators investigated.The true positive wasting cases had a mean mid-upper arm circumference(MUAC)of 14.6 cm,which may explain the high false negative rate found in terms of children identified with wasting,where ECD practitioners fail to use the weight for height Z-score(WHZ)interpretation for screening.Conclusion:By using ECD centres as hub to screen for malnutrition,it may contribute to the early identification of failure to thrive among young children.Although it was concerning that trained ECD practitioners are missing some children with an unacceptably high false negative rate,it may have been due to the fact that wasting in older children cannot be identified with MUAC alone and that accurate WFH plotting is needed.Onsite mentorship by governmental health workers may provide ECD practitioners with more confidence to screen children for growth failure based on regular WFH measurements.Moreover,ECD practitioners will be more confident to monitor the Road to Health booklets for missed vaccinations,vitamin A and deworming opportunities.
文摘Cell polarization and asymmetric cell divisions play important roles during development in many multicellular eukaryotes. Fucoid algae have a long history as models for studying early developmental processes, probably because of the ease with which zygotes can be observed and manipulated in the laboratory. This review discusses cell polarization and asymmetric cell divisions in fucoid algal zygotes with an emphasis on the roles played by the cytoskeleton.
文摘Objective: to explore the effect of early intervention nursing measures to promote the development of infant growth and development in children. Methods: in order to comprehensively improve the early growth and development of children and implement the intervention nursing measures, 80 cases of infants who visited our department from July 2019 to July 2020 were selected and divided into the control group and the research group. The control group received routine early childhood care. On the basis of the control group, the research group adopted the child health care intervention nursing measures to give full play to the influence of the two groups in caring for the growth and development of children. Results: effective analysis of the physical conditions of infants in different groups temporarily after the 12-month intervention nursing intervention showed that their average height was (76.85±6.27)cm, and the data from the comprehensive analysis study group were significantly superior to those of the control group (73.02±1.93)cm. The data on the related clinical nursing intervention effects of infants in the two groups were statistically significant (P < 0.05). In terms of NET score, by exploring the growth and development of infants in two groups, the score data of the research group at 6 months, 9 months and 12 months were significantly better than the application related data of the control group. The difference between the two groups was statistically significant (P < 0.05). Conclusion: the effective combination of early implementation of scientific management interventions in two groups of infants significantly promotes the growth and development of children in all aspects. Through comparative analysis, the growth and development as well as the intelligence development of infants measured by the growth and development status of infants in the research group are significantly better than those in the control group, which is worthy of clinical promotion.
基金supported by Talent Research Foundation of Henan University of Technology (09001095)Scientific and Technological Project of Henan Province " Research and Demonstration of Supporting Production Technology Systemfor High-quality Funiu White Goats" (072102130004)
文摘[ Objective] To study the growth-development of Boer goats from embryo transplant. [ Method] With Boer goats from embryo transplant as the tested animals and natural breeding Boer goats born in the same period as CK, the weight of Boer goats at birth and at the age of 1,3 and 6 months as well as the body size at the age of 3 and 6 months were investigated in this study, while their health conditions were also observed and recorded. [ Result] Under the normal feeding conditions, Boer goats from embryo transplant developed well at early stage, and their meat-using body type were also obvious. The average birth weight of bucks was （4.25±0.95） kg, and that of does was （3.74±0.10） kg. The average weight of bucks at the age of 6 months was （31.90 ±0.74） kg and that of was （25.90 ±0.67） kg. The daily gains of bucks and ewes from birth to 6 months of age were 153.64 and 123.11 g respectively, while their body length, body height and chest circumference increased obviously, and there was no significant difference in body weight or body size between them and natural breeding goats. [ Condusioa] The study lays a foundation for further studies on the breedinq and utilization of Boer noats from embryo transplant at early staae.
基金Supported by Heilongjiang Provincial Natural Science Foundation(Face Project)(C2016021)Open Project of Key Laboratory of Feed Science,Northeast Agricultural University,Heilongjiang Province(yy-2012-10)。
文摘RanBP1 is a binding protein of Ran that plays a pivotal role in nucleocytoplasmic transport.In this study,the localization and possible functions of RanBP1 were examined,during the early embryonic development of mice.Immunofluorescence results showed that RanBP1 was mainly localized in cytoplasm at mitosis interphase,and its concentration was lower in nucleus and the lowest in nucleolus.With the formation of the spindle in the early embryonic cells,RanBP1 condensed area took the shape of spindle microtubule,the concentration of RanBP1 was low in the site of chromosome.During the formation of nucleus,RanBP1 concentrated in nucleus and there were few dots of RanBP1 around the nucleolus.These dots were lost after the nucleus full growth.The results showed that RanBP1 had important roles in nucleocytoplasmic transport,spindle formation and nuclear assembly in the early embryonic development of mice.
文摘The paper sought to explore issues of access, equity, and quality in the early childhood development （ECD） sub-sector in Zimbabwe. The massive expansion of ECD services prompted the author to undertake the study. Data were collected using focus group discussions, individual interviews, document analysis, and observations. Information from literature, research reports, policies, and practices was also analyzed in order to establish the state of affairs in the provision of ECD. The data were analyzed using theme identification methods to solicit emerging issues. Generally, it was found that on issues of access, equity, and quality in ECD in Zimbabwe, there is a quality-quantity dilemma with qualitative progress less conspicuous than quantitative progress. The resultant specific challenges include： large teacher-child ratio; lack of parental involvement, lack of trained ECD specialists with expertise in using a play-based curriculum; and shortage of equipment, material resources, and age appropriate infrastructure. In most ECD settings, there were inadequate health and nutrition provisions. Water, sanitation and health （WASH） facilities were found to be insufficient in most centres. The study recommended that there is need for： equal access to quality early childhood education; provision of infrastructure, provision of ability and age appropriate resources; and health, sanitation, and nutrition services especially in rural settings. Overall, ECD programmes in Zimbabwe should strive to attain quality more than quantity services. The need to train more early childhood specialists who would drive the ECD sub-sector is emphasized.
