In this study,an online HPLC-DAD-MS coupled with 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)diammonium salt(A BTS)assay was employed for evaluating free radical scavenging activity of Eagle tea and their ...In this study,an online HPLC-DAD-MS coupled with 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)diammonium salt(A BTS)assay was employed for evaluating free radical scavenging activity of Eagle tea and their active components.Twenty-three chromatographic peaks were detected,and nineteen components had free radical scavenging activity.Among them,eight compounds were identified as flavonoids(hyperin,isoquercitrin,quercitrin,quercetin,kaempferol),catechins,chlorogenic acid and epicatechin based on MS data and standard chromatographic characters.展开更多
基金The research was supported by a grant from University of Macao(UL015/09-Y3 to Shaoping Li).
文摘In this study,an online HPLC-DAD-MS coupled with 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)diammonium salt(A BTS)assay was employed for evaluating free radical scavenging activity of Eagle tea and their active components.Twenty-three chromatographic peaks were detected,and nineteen components had free radical scavenging activity.Among them,eight compounds were identified as flavonoids(hyperin,isoquercitrin,quercitrin,quercetin,kaempferol),catechins,chlorogenic acid and epicatechin based on MS data and standard chromatographic characters.