详细解读道达尔GS EP PLR 205标准的主要内容。分析认为,与API Spec 5L标准相比,GS EP PLR205标准对化学成分要求更严,提出了偏析控制的要求;对性能如纵横向拉伸、冲击、硬度、氢致开裂和硫化物应力腐蚀开裂和应变时效等要求做了规定;...详细解读道达尔GS EP PLR 205标准的主要内容。分析认为,与API Spec 5L标准相比,GS EP PLR205标准对化学成分要求更严,提出了偏析控制的要求;对性能如纵横向拉伸、冲击、硬度、氢致开裂和硫化物应力腐蚀开裂和应变时效等要求做了规定;尺寸偏差特别是对管端内径、内径不圆度要求更严;对探伤检验的人员资质、校样要求和探伤方式进行了详细规定。解读GS EP PLR 205标准有利于制造商对标一线要求,寻找差距,改进工艺和控制能力,为进入国际高端市场做准备。展开更多
Deep oil and gas reservoirs exist under high-temperature conditions.In situ temperature-preserved coring(ITP-Coring)is an innovative and crucial method for evaluating and exploiting deep oil and gas resources.Thermal ...Deep oil and gas reservoirs exist under high-temperature conditions.In situ temperature-preserved coring(ITP-Coring)is an innovative and crucial method for evaluating and exploiting deep oil and gas resources.Thermal insulation materials are key to achieving successful ITP-Coring.Materials composed of hollow glass microspheres(HGMs)as fillers and epoxy resin(EP)as the matrix are promising thermal insulation materials for application in ITP-Coring to exploit deep resources.The compressive mechanical properties of these materials significantly influence their applicability and reliability.However,few studies have focused on the compressive mechanical behavior of the materials under high-temperature and high-pressure(HTHP)coupled conditions.Therefore,compressive mechanical tests on materials under temperatures and pressures of up to 150℃and 140 MPa were conducted innovatively.The compressive stress-strain curves of the materials were divided into three stages:elastic,yield,and failure,at temperatures ranging from 25℃to 100℃.Increasing temperature and pressure resulted in a decrease in compressive mechanical properties.Notably,high pressure damaged the HGMs,increasing compressive strain as the temperature rose.Additionally,the compressive failure mode shifted from compound failure to shear failure at different thresholds of HTHP conditions.Finally,a constitutive model of compressive mechanics that considered multiple coupled factors was established,demonstrating good agreement with the experimental results.These findings provide both experimental and theoretical support for the optimization and engineering application of HGMs/EP materials.展开更多
目的:探讨乳酸对三阴性乳腺癌(triple-negative breast cancer,TNBC)细胞恶性行为的影响,并揭示胰岛素样生长因子2 mRNA结合蛋白3(insulin-like growth factor 2 m RNA binding protein 3,IGF2BP3)通过m6A依赖性方式调控EP300,进而介导...目的:探讨乳酸对三阴性乳腺癌(triple-negative breast cancer,TNBC)细胞恶性行为的影响,并揭示胰岛素样生长因子2 mRNA结合蛋白3(insulin-like growth factor 2 m RNA binding protein 3,IGF2BP3)通过m6A依赖性方式调控EP300,进而介导代谢重编程的表观遗传学机制。方法:通过外源性添加乳酸钠处理TNBC细胞,采用CCK-8、集落形成、划痕愈合和Transwell实验检测细胞增殖与迁移能力;利用shRNA慢病毒载体构建IGF2BP3稳定敲低细胞模型,结合Western blot分析组蛋白乳酸化修饰水平;进一步通过RIP-seq、MeRIP-seq筛选IGF2BP3的m6A靶基因,并利用qRT-PCR和Western blot验证关键分子表达。结果:乳酸钠处理显著增强了TNBC细胞的增殖和迁移能力,且泛乳酸化水平随乳酸浓度梯度升高而增加。IGF2BP3敲低可降低组蛋白乳酸化修饰水平。通过整合RIP-seq和MeRIP-seq数据,鉴定出699个其转录本同时被IGF2BP3特异性结合且携带m6A修饰的候选基因,这些基因显著富集于表观遗传调控和代谢相关通路。EP300转录本上IGF2BP3的结合区域与m6A修饰位点高度重合,且EP300的表达水平在IGF2BP3敲低后明显下调。结论:外源性乳酸通过泛乳酸化修饰促进TNBC的恶性进展。IGF2BP3通过m6A依赖性机制调控EP300的表达,进而介导组蛋白乳酸化修饰与代谢重编程的协同作用,最终驱动TNBC的肿瘤进展。上述发现为靶向代谢-表观遗传交互调控的新型治疗策略提供了理论依据。展开更多
文摘详细解读道达尔GS EP PLR 205标准的主要内容。分析认为,与API Spec 5L标准相比,GS EP PLR205标准对化学成分要求更严,提出了偏析控制的要求;对性能如纵横向拉伸、冲击、硬度、氢致开裂和硫化物应力腐蚀开裂和应变时效等要求做了规定;尺寸偏差特别是对管端内径、内径不圆度要求更严;对探伤检验的人员资质、校样要求和探伤方式进行了详细规定。解读GS EP PLR 205标准有利于制造商对标一线要求,寻找差距,改进工艺和控制能力,为进入国际高端市场做准备。
基金supported by the National Key Research and Development Program of China(Grant No.2023YFB2390200)the National Natural Science Foundation of China(Grant No.52304033)the Guangdong Provincial Key Laboratory of Deep Earth Sciences and Geothermal Energy Exploitation and Utilization(Grant No.DESGEEU-2023-10).
文摘Deep oil and gas reservoirs exist under high-temperature conditions.In situ temperature-preserved coring(ITP-Coring)is an innovative and crucial method for evaluating and exploiting deep oil and gas resources.Thermal insulation materials are key to achieving successful ITP-Coring.Materials composed of hollow glass microspheres(HGMs)as fillers and epoxy resin(EP)as the matrix are promising thermal insulation materials for application in ITP-Coring to exploit deep resources.The compressive mechanical properties of these materials significantly influence their applicability and reliability.However,few studies have focused on the compressive mechanical behavior of the materials under high-temperature and high-pressure(HTHP)coupled conditions.Therefore,compressive mechanical tests on materials under temperatures and pressures of up to 150℃and 140 MPa were conducted innovatively.The compressive stress-strain curves of the materials were divided into three stages:elastic,yield,and failure,at temperatures ranging from 25℃to 100℃.Increasing temperature and pressure resulted in a decrease in compressive mechanical properties.Notably,high pressure damaged the HGMs,increasing compressive strain as the temperature rose.Additionally,the compressive failure mode shifted from compound failure to shear failure at different thresholds of HTHP conditions.Finally,a constitutive model of compressive mechanics that considered multiple coupled factors was established,demonstrating good agreement with the experimental results.These findings provide both experimental and theoretical support for the optimization and engineering application of HGMs/EP materials.
文摘目的:探讨乳酸对三阴性乳腺癌(triple-negative breast cancer,TNBC)细胞恶性行为的影响,并揭示胰岛素样生长因子2 mRNA结合蛋白3(insulin-like growth factor 2 m RNA binding protein 3,IGF2BP3)通过m6A依赖性方式调控EP300,进而介导代谢重编程的表观遗传学机制。方法:通过外源性添加乳酸钠处理TNBC细胞,采用CCK-8、集落形成、划痕愈合和Transwell实验检测细胞增殖与迁移能力;利用shRNA慢病毒载体构建IGF2BP3稳定敲低细胞模型,结合Western blot分析组蛋白乳酸化修饰水平;进一步通过RIP-seq、MeRIP-seq筛选IGF2BP3的m6A靶基因,并利用qRT-PCR和Western blot验证关键分子表达。结果:乳酸钠处理显著增强了TNBC细胞的增殖和迁移能力,且泛乳酸化水平随乳酸浓度梯度升高而增加。IGF2BP3敲低可降低组蛋白乳酸化修饰水平。通过整合RIP-seq和MeRIP-seq数据,鉴定出699个其转录本同时被IGF2BP3特异性结合且携带m6A修饰的候选基因,这些基因显著富集于表观遗传调控和代谢相关通路。EP300转录本上IGF2BP3的结合区域与m6A修饰位点高度重合,且EP300的表达水平在IGF2BP3敲低后明显下调。结论:外源性乳酸通过泛乳酸化修饰促进TNBC的恶性进展。IGF2BP3通过m6A依赖性机制调控EP300的表达,进而介导组蛋白乳酸化修饰与代谢重编程的协同作用,最终驱动TNBC的肿瘤进展。上述发现为靶向代谢-表观遗传交互调控的新型治疗策略提供了理论依据。
