LVLPGE is an angiotensin-converting enzyme(ACE)inhibitory peptide extracted from broccoli protein hydrolysates.The antihypertensive mechanism of LVLPGE was explored in the present study.The results indicated that the ...LVLPGE is an angiotensin-converting enzyme(ACE)inhibitory peptide extracted from broccoli protein hydrolysates.The antihypertensive mechanism of LVLPGE was explored in the present study.The results indicated that the IC50 value of LVLPGE was 19.49μM,and its binding to ACE occurred via a static fluorescence quenching involving the secondary structure changes of ACE.In addition,LVLPGE competitively bound to the active site of ACE through hydrogen bonding.Furthermore,LVLPGE markedly enhanced the levels of the vasodilator nitric oxide(NO)in the Ang I-induced HUVECs model,suppressed the expression of vasoconstrictor endothelin-1(ET-1),and increased the phosphorylation of eNOS.Moreover,LVLPGE was found to enhance the production of cyclic guanosine monophosphate(cGMP),which leading to a subsequent decrease in intracellular calcium concentration,ultimately facilitating the relaxation of vascular smooth muscles and lowering the blood pressure.These results demonstrated that LVLPGE achieves the antihypertensive effects by inhibiting ACE activity and activating the eNOS/NO/cGMP signaling pathway.This study provides new insights into the antihypertensive mechanism of LVLPGE and contributes to the high-value utilization of broccoli resources.展开更多
Red ginseng has been used to ameliorate erectile dysfunction(ED),a common age-related male reproductive disorder,though the active constituents responsible for this effect remain unclear.Arginyl-fructosyl-glucose(AFG)...Red ginseng has been used to ameliorate erectile dysfunction(ED),a common age-related male reproductive disorder,though the active constituents responsible for this effect remain unclear.Arginyl-fructosyl-glucose(AFG)and arginyl-fructose(FA),are two arginine-derived Amadori compounds formed via Maillard reaction during red ginseng processing.An optimized vacuum dehydration method yielded 64.58%and 87.95%,respectively for AFG and FA,a significant increase compared to previous reported best results.In D-galactoseinduced aging ED rats,the prepared AFG and FA significantly improved erectile function by reducing fibrosis and alleviating oxidative stress in the penile corpus cavernosum(PCC),alongside up-regulation of the eNOS/NO/cGMP signaling pathway within PCC.AFG and FA co-administration with 20(S)-ginsenoside Rg3 further enhanced the efficacy.These findings identify AFG and FA as key bioactives mediating the ED-improving effects of red ginseng and provide novel insights for red ginseng-based functional food development.展开更多
基金Grants from National Key R&D Program of China(2022YFF11002)Zhejiang Provincial Natural Science Foundation(LQ23C200009)National Natural Science Foundation of China(32302052).
文摘LVLPGE is an angiotensin-converting enzyme(ACE)inhibitory peptide extracted from broccoli protein hydrolysates.The antihypertensive mechanism of LVLPGE was explored in the present study.The results indicated that the IC50 value of LVLPGE was 19.49μM,and its binding to ACE occurred via a static fluorescence quenching involving the secondary structure changes of ACE.In addition,LVLPGE competitively bound to the active site of ACE through hydrogen bonding.Furthermore,LVLPGE markedly enhanced the levels of the vasodilator nitric oxide(NO)in the Ang I-induced HUVECs model,suppressed the expression of vasoconstrictor endothelin-1(ET-1),and increased the phosphorylation of eNOS.Moreover,LVLPGE was found to enhance the production of cyclic guanosine monophosphate(cGMP),which leading to a subsequent decrease in intracellular calcium concentration,ultimately facilitating the relaxation of vascular smooth muscles and lowering the blood pressure.These results demonstrated that LVLPGE achieves the antihypertensive effects by inhibiting ACE activity and activating the eNOS/NO/cGMP signaling pathway.This study provides new insights into the antihypertensive mechanism of LVLPGE and contributes to the high-value utilization of broccoli resources.
基金supported by the grants from Thirteen Five National Key Research and Development Program of China(2018YFD0400902)Changji science and technology project(2019G02).
文摘Red ginseng has been used to ameliorate erectile dysfunction(ED),a common age-related male reproductive disorder,though the active constituents responsible for this effect remain unclear.Arginyl-fructosyl-glucose(AFG)and arginyl-fructose(FA),are two arginine-derived Amadori compounds formed via Maillard reaction during red ginseng processing.An optimized vacuum dehydration method yielded 64.58%and 87.95%,respectively for AFG and FA,a significant increase compared to previous reported best results.In D-galactoseinduced aging ED rats,the prepared AFG and FA significantly improved erectile function by reducing fibrosis and alleviating oxidative stress in the penile corpus cavernosum(PCC),alongside up-regulation of the eNOS/NO/cGMP signaling pathway within PCC.AFG and FA co-administration with 20(S)-ginsenoside Rg3 further enhanced the efficacy.These findings identify AFG and FA as key bioactives mediating the ED-improving effects of red ginseng and provide novel insights for red ginseng-based functional food development.
