[Objective] The aim was to investigate the possible interaction between SET and eEF1A1 in human liver cells. [Method] Firstly the total proteins of human L-02 liver cells were extracted under non-denaturing conditions...[Objective] The aim was to investigate the possible interaction between SET and eEF1A1 in human liver cells. [Method] Firstly the total proteins of human L-02 liver cells were extracted under non-denaturing conditions; then,mouse anti-human SET and rabbit anti-human eEF1A1 antibodies were used to perform the co-immunoprecipitation respectively; subsequently,the immunoprecipitations was correspondingly detected with rabbit anti-human eEF1A1 and mouse anti-human SET antibodies by Western Blot. [Result] EEF1A1 was detected in protein complex from the immunoprecipitations by using anti-SET antibody,and SET also was detected in immunoprecipitations by using anti-eEF1A1 antibody. [Conclusion] The interaction between SET and eEF1A1 in human liver cells was confirmed.展开更多
Objective: To investigate the effects of eEF1A1 expression on cancer cell load and proliferation activity in lung cancer lesions. Methods: A total of 200 patients with non-small cell lung cancer who received radical o...Objective: To investigate the effects of eEF1A1 expression on cancer cell load and proliferation activity in lung cancer lesions. Methods: A total of 200 patients with non-small cell lung cancer who received radical operation for lung cancer in our hospital were enrolled in lung cancer group, and 78 patients with pulmonary bullae who accepted emergency surgical treatment in our hospital during the same period were enrolled in pulmonary bullae group. Differences in eEF1A1 and proliferation gene expression in lesion tissue were compared between the two groups, and the contents of serum tumor markers and angiogenesis indexes were detected. Pearson test was used to evaluate the correlation of eEF1A1 expression with tumor markers, angiogenesis indexes and proliferation genes in the lesions of patients with non-small cell lung cancer. Results: eEF1A1 expression in lesion tissue of lung cancer group was higher than that of pulmonary bullae group;serum CYFRA21-1, ProGRP, CEA, SCC Ag, HIF-1 and VEGF levels of lung cancer group were higher than those of pulmonary bullae group and positively correlated with the eEF1A1 expression;LRRC3B, TUSC3 and VPS33B mRNA expression in lesion tissue of lung cancer group were lower than those of pulmonary bullae group and negatively correlated with the eEF1A1 expression whereas MACC1 and RACK1 mRNA expression were higher than those of pulmonary bullae group and positively correlated with the eEF1A1 expression. Conclusion: eEF1A1 is highly expressed in non-small cell lung cancer tissues, and the specific expression can objectively reflect the tumor load and the proliferation activity of tumor cells.展开更多
基金Supported by National Natural Science Foundation (30972454)Shenzhen Science and Technology Plan Major Project (200801010)~~
文摘[Objective] The aim was to investigate the possible interaction between SET and eEF1A1 in human liver cells. [Method] Firstly the total proteins of human L-02 liver cells were extracted under non-denaturing conditions; then,mouse anti-human SET and rabbit anti-human eEF1A1 antibodies were used to perform the co-immunoprecipitation respectively; subsequently,the immunoprecipitations was correspondingly detected with rabbit anti-human eEF1A1 and mouse anti-human SET antibodies by Western Blot. [Result] EEF1A1 was detected in protein complex from the immunoprecipitations by using anti-SET antibody,and SET also was detected in immunoprecipitations by using anti-eEF1A1 antibody. [Conclusion] The interaction between SET and eEF1A1 in human liver cells was confirmed.
文摘Objective: To investigate the effects of eEF1A1 expression on cancer cell load and proliferation activity in lung cancer lesions. Methods: A total of 200 patients with non-small cell lung cancer who received radical operation for lung cancer in our hospital were enrolled in lung cancer group, and 78 patients with pulmonary bullae who accepted emergency surgical treatment in our hospital during the same period were enrolled in pulmonary bullae group. Differences in eEF1A1 and proliferation gene expression in lesion tissue were compared between the two groups, and the contents of serum tumor markers and angiogenesis indexes were detected. Pearson test was used to evaluate the correlation of eEF1A1 expression with tumor markers, angiogenesis indexes and proliferation genes in the lesions of patients with non-small cell lung cancer. Results: eEF1A1 expression in lesion tissue of lung cancer group was higher than that of pulmonary bullae group;serum CYFRA21-1, ProGRP, CEA, SCC Ag, HIF-1 and VEGF levels of lung cancer group were higher than those of pulmonary bullae group and positively correlated with the eEF1A1 expression;LRRC3B, TUSC3 and VPS33B mRNA expression in lesion tissue of lung cancer group were lower than those of pulmonary bullae group and negatively correlated with the eEF1A1 expression whereas MACC1 and RACK1 mRNA expression were higher than those of pulmonary bullae group and positively correlated with the eEF1A1 expression. Conclusion: eEF1A1 is highly expressed in non-small cell lung cancer tissues, and the specific expression can objectively reflect the tumor load and the proliferation activity of tumor cells.
