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Formation and Evolution of Stars and Galaxies in the Cosmic Environment 被引量:1
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作者 Cui-xiang Zhong 《Journal of Environmental Science and Engineering(B)》 2025年第2期53-64,共12页
With the advancement of astronomical observation technology,people have a deeper understanding of the formation and evolution of galaxies,but many details of our own Milky Way and other external galaxies are still unk... With the advancement of astronomical observation technology,people have a deeper understanding of the formation and evolution of galaxies,but many details of our own Milky Way and other external galaxies are still unknown.Therefore,by studying the formation and orbital transformation mechanism of satellites,planets and stars,the author puts forward a new theory on the formation and evolution of stars and galaxies,thus revealing the hierarchical structure of galaxies and the formation and evolution laws of main sequence stars,red giants,white dwarfs,black dwarfs,supernovae,neutron stars,black holes and quasars.Some special phenomena in the course of star formation and evolution,such as sunspots,flares,fast radio bursts and gamma-ray bursts,have also been revealed. 展开更多
关键词 SUNSPOT red giant White Dwarf SUPERNOVA neutron star QUASAR fast radio burst gamma-ray burst.
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The phytohormone jasmonic acid is involved in rice resistance to Southern rice black-streaked dwarf virus 被引量:1
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作者 Chaorui Huang Qing Liu +7 位作者 Qingling Qi Chenfei Gao Lulu Li Yanjun Li Jianping Chen Zongtao Sun Jianlong Xu Hehong Zhang 《The Crop Journal》 2025年第1期23-30,共8页
Viruses are significant pathogens causing severe plant infections and crop losses globally.The resistance mechanisms of rice to viral diseases,particularly Southern rice black-streaked dwarf virus(SRBSDV),remain poorl... Viruses are significant pathogens causing severe plant infections and crop losses globally.The resistance mechanisms of rice to viral diseases,particularly Southern rice black-streaked dwarf virus(SRBSDV),remain poorly understood.In this study,we assessed SRBSDV susceptibility in 20 Xian/indica(XI)and 20 Geng/japonica(GJ)rice varieties.XI-1B accessions in the Xian subgroup displayed higher resistance than GJ accessions.Comparative transcriptome analysis revealed changes in processes like oxidoreductase activity,jasmonic acid(JA)metabolism,and stress response.JA sensitivity assays further linked antiviral defense to the JA pathway.These findings highlight a JA-mediated resistance mechanism in rice and offer insights for breeding SRBSDV-resistant varieties. 展开更多
关键词 Oryza sativa(rice) Jasmonic acid Southern rice black-streaked dwarf virus(SRBSDV) Variety resistance Defense response
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萱草独脚金内酯合成基因HfD27的克隆与表达分析
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作者 蒋成娣 王艺程 +4 位作者 丁寒雪 陈荣洋 曹俊峰 张志国 刘翔 《分子植物育种》 北大核心 2025年第11期3563-3573,共11页
独脚金内酯(strigolactones,SLs)通过抑制分枝的作用对植物进行株型调控,DWARF27(D27)是独脚金内酯生物合成过程的关键基因。本研究于实验室已有的萱草三代转录组数据,根据水稻、拟南芥D27基因从萱草三代转录组数据中鉴定和筛选萱草D27... 独脚金内酯(strigolactones,SLs)通过抑制分枝的作用对植物进行株型调控,DWARF27(D27)是独脚金内酯生物合成过程的关键基因。本研究于实验室已有的萱草三代转录组数据,根据水稻、拟南芥D27基因从萱草三代转录组数据中鉴定和筛选萱草D27候选基因,命名为HfD27。利用PCR技术,在萱草品种‘秋红’中克隆得到HfD27基因的CDS全长。亚细胞定位结果显示HfD27定位于叶绿体中,荧光定量PCR表明HfD27在萱草腋芽的表达量最高。本研究对于解析独脚金内酯在萱草内的生物合成路径以及HfD27基因对于分蘖的调控功能具有一定参考价值。 展开更多
关键词 萱草 独脚金内酯 DWARF27 分蘖 生物合成
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PURINE PERMEASE 4 regulates plant height in maize
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作者 Yuchen Huang Yuehui Zhang +1 位作者 Xiaofeng Cai Shui Wang 《Journal of Genetics and Genomics》 2025年第3期446-448,共3页
Plant height is an important trait that affects the crop yield and overall productivity.The Green Revolution,which began in the 1960s,brought about a remarkable surge in grain production,largely credited to the introd... Plant height is an important trait that affects the crop yield and overall productivity.The Green Revolution,which began in the 1960s,brought about a remarkable surge in grain production,largely credited to the introduction of new wheat(Triticum aestivum)and rice(Oryza sativa)varieties,specifically the dwarf variants.Short plants offer several advantages,including denser planting,resistance to lodging,and easier application of fertilizers or fungicides(Stokstad,2023). 展开更多
关键词 plant height crop yield productivity green revolution dwarf variantsshort MAIZE green revolutionwhich purine permease
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Natural variation of ClCCT promoter promotes flowering and dwarfing in adlay(Coix lacryma-jobi L.)
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作者 Jieliang Bao Yanan Wang +11 位作者 Kuoshu Cui Xiangdong Li Hong Pan Chaoyuan Shi Xiang Yan Jinglin Wang Xiaotong Wang Qing Dou Xiaolong Dong Yibing Yuan Shufeng Zhou Chao Guo 《The Crop Journal》 2025年第3期942-951,共10页
The flowering time is a key trait that determines adaptation,yield and quality of crops.Adlay,a traditional cereal crop,has developed a distinctive agricultural industry in Southwest China and Southeast Asian countrie... The flowering time is a key trait that determines adaptation,yield and quality of crops.Adlay,a traditional cereal crop,has developed a distinctive agricultural industry in Southwest China and Southeast Asian countries.However,the currently planted varieties are all semi-domesticated landraces with delayed flowering and excessive height.These defects limit yield improvement per unit area and mechanized harvesting.In this study,a major QTL associated with flowering time and plant height in adlay has been mapped and identified as Cl CCT,a gene having a conserved function and regulatory pathway for inhibiting flowering time and increasing plant height in gramineous crops.Among the six identified haplotypes of Cl CCT,the haplotype with 38-bp insertion in promoter region of Cl CCT has earlier flowering time and wider geographical distribution than other haplotypes.The insertion variation,which arises from the segmental duplication of Cl CCT,can inhibit the expression level of reporter gene and has been used in breeding for early maturity and dwarfing.These research results not only reinforce our understanding of the importance of CCT domain protein in the tropical crops adapting to high-latitude environment,but also provide a validated breeding target for the early maturity and dwarfing of adlay. 展开更多
关键词 Early maturity DWARFING CCT domain Convergent selection Genome duplication event
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TCP J07222683+6220548:A New AM CVn Type System with Infrequent Outbursts
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作者 Alexander Tarasenkov Kirill Sokolovsky +11 位作者 Alexandr Dodin Oxana Chernyshenko Stanislav Korotkiy Ivan Strakhov Marina Burlak Sergey Naroenkov Franz-Josef Hambsch Tamás Tordai Hiroshi Itoh Yasuo Sano Yusuke Tampo Ferdinand 《Research in Astronomy and Astrophysics》 2025年第7期189-202,共14页
We present the discovery of TCP J07222683+6220548,a new ultracompact binary system of the AM CVn type.This system was first identified displaying aΔV=7.6 mag outburst on 2025-01-20.9416 UTC by the New Milky Way wide-... We present the discovery of TCP J07222683+6220548,a new ultracompact binary system of the AM CVn type.This system was first identified displaying aΔV=7.6 mag outburst on 2025-01-20.9416 UTC by the New Milky Way wide-field survey for transients and later independently detected by ASAS-SN and ZTF.The outburst peaked at V_(max)=12.45 and lasted for seven days,followed by a series of rebrightenings.No previous outbursts are found in archival data.Positive superhumps with a period of 0.032546±0.000084 day(46.87±0.12 minutes),barely detectable during the main outburst,became clearly visible during the first rebrightening that lasted from day 18 to day 24 after the initial outburst.No convincing change in the superhump period was detected.Dense time-series photometry follow-up by a pair of 0.5 m INASAN robotic telescopes,together with VSNET and AAVSO observers,was essential for identifying TCP J07222683+6220548 as an AM CVn system and triggering confirmation spectroscopy with the 2.5 m CMO SAI telescope.Some outbursting AM CVn systems lacking such detailed follow-up may remain unrecognized among the newly discovered cataclysmic variable candidates. 展开更多
关键词 (stars:)white dwarfs (stars:)novae cataclysmic variables stars:dwarf novae stars:individual(TCP J07222683+6220548)
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Star Formation Efficiency of Blue Compact Galaxies Through(OⅡ),(Hα),(IR),and 1.4 GHz Radio Continuum
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作者 Gemechu Muleta Kumssa 《Research in Astronomy and Astrophysics》 2025年第2期142-149,共8页
This paper investigates the star formation efficiency(SFE)of blue compact galaxies(BCGs)by analyzing various indicators,including(O II),(H_(α)),infrared emissions,and the 1.4 GHz radio continuum.The assessment of SFE... This paper investigates the star formation efficiency(SFE)of blue compact galaxies(BCGs)by analyzing various indicators,including(O II),(H_(α)),infrared emissions,and the 1.4 GHz radio continuum.The assessment of SFE was conducted using publicly accessible data that concentrated on star formation rates(SFRs)and depletion timescales.By employing the fundamental equation,I determined the SFE in relation to its freefall time,which highlighted variations in efficiencies associated with SFR masses derived from different spectra.The results demonstrated that the highest efficiency value was observed in the analysis of the 1.4 GHz radio continuum,emphasizing the importance of studying star-forming clouds across a range of electromagnetic wavelengths to achieve a comprehensive understanding of star formation mechanisms.The study's findings indicated a peak efficiency of star formation value of approximately 0.9%,while the relative minimum obtained from(O II)was around 0.42%,aligning with the range of SFEs documented in the existing literature.Additionally,investigating the factors that influence the variability in efficiency when examining clouds in BCGs across different wave bands is crucial for deepening our comprehension of the underlying processes of star formation. 展开更多
关键词 catalogs methods:analytical TURBULENCE shock waves accretion accretion disks stars:dwarf novae
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Enhanced Multi-Object Dwarf Mongoose Algorithm for Optimization Stochastic Data Fusion Wireless Sensor Network Deployment
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作者 Shumin Li Qifang Luo Yongquan Zhou 《Computer Modeling in Engineering & Sciences》 2025年第2期1955-1994,共40页
Wireless sensor network deployment optimization is a classic NP-hard problem and a popular topic in academic research.However,the current research on wireless sensor network deployment problems uses overly simplistic ... Wireless sensor network deployment optimization is a classic NP-hard problem and a popular topic in academic research.However,the current research on wireless sensor network deployment problems uses overly simplistic models,and there is a significant gap between the research results and actual wireless sensor networks.Some scholars have now modeled data fusion networks to make them more suitable for practical applications.This paper will explore the deployment problem of a stochastic data fusion wireless sensor network(SDFWSN),a model that reflects the randomness of environmental monitoring and uses data fusion techniques widely used in actual sensor networks for information collection.The deployment problem of SDFWSN is modeled as a multi-objective optimization problem.The network life cycle,spatiotemporal coverage,detection rate,and false alarm rate of SDFWSN are used as optimization objectives to optimize the deployment of network nodes.This paper proposes an enhanced multi-objective mongoose optimization algorithm(EMODMOA)to solve the deployment problem of SDFWSN.First,to overcome the shortcomings of the DMOA algorithm,such as its low convergence and tendency to get stuck in a local optimum,an encircling and hunting strategy is introduced into the original algorithm to propose the EDMOA algorithm.The EDMOA algorithm is designed as the EMODMOA algorithm by selecting reference points using the K-Nearest Neighbor(KNN)algorithm.To verify the effectiveness of the proposed algorithm,the EMODMOA algorithm was tested at CEC 2020 and achieved good results.In the SDFWSN deployment problem,the algorithm was compared with the Non-dominated Sorting Genetic Algorithm II(NSGAII),Multiple Objective Particle Swarm Optimization(MOPSO),Multi-Objective Evolutionary Algorithm based on Decomposition(MOEA/D),and Multi-Objective Grey Wolf Optimizer(MOGWO).By comparing and analyzing the performance evaluation metrics and optimization results of the objective functions of the multi-objective algorithms,the algorithm outperforms the other algorithms in the SDFWSN deployment results.To better demonstrate the superiority of the algorithm,simulations of diverse test cases were also performed,and good results were obtained. 展开更多
关键词 Stochastic data fusion wireless sensor networks network deployment spatiotemporal coverage dwarf mongoose optimization algorithm multi-objective optimization
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Eleven Local Volume Dwarf Galaxies in the FASHI Survey
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作者 Aleksandra Nazarova Dmitry Makarov +2 位作者 Igor Karachentsev Chuan-Peng Zhang Ming Zhu 《Research in Astronomy and Astrophysics》 2025年第12期157-166,共10页
We determined HI parameters for eleven nearby late-type dwarf galaxies using FASHI data cubes,despite the fact that the first version of the FAS HI catalog does not list any radio sources that could correspond to thes... We determined HI parameters for eleven nearby late-type dwarf galaxies using FASHI data cubes,despite the fact that the first version of the FAS HI catalog does not list any radio sources that could correspond to these galaxies.Four of them are probable peripheral satellites of the bright spiral galaxies:NGC 3556,NGC 4258,NGC 4274 and NGC 4490,while others are isolated objects.The considered sample has the following median parameters:a heliocentric velocity of V_(h)=542 km s^(-1),an HI-line width of W_(50)=28 km s^(-1),a hydrogen mass of log(M_(HI)/M_(⊙))=6.83,a stellar mass of log(M_(*)/M_(⊙))=7.19,and a specific star formation rate of sSFR=-10.17 yr^(-1). 展开更多
关键词 galaxies:dwarf surveys galaxies:distances and redshifts
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Properties of Standard,Fuzzy,and Self-interacting Dark Matter Haloes in Dwarf Galaxies
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作者 Fahmi M.Al Farisy Hesti R.T.Wulandari Azriel J.Dante 《Research in Astronomy and Astrophysics》 2025年第4期194-208,共15页
This study examines the properties of standard cold dark matter(CDM),fuzzy dark matter(FDM),and selfinteracting dark matter(SIDM)haloes by analyzing the rotation curves of selected dwarf galaxies from SPARC and LITTLE... This study examines the properties of standard cold dark matter(CDM),fuzzy dark matter(FDM),and selfinteracting dark matter(SIDM)haloes by analyzing the rotation curves of selected dwarf galaxies from SPARC and LITTLE THINGS in 3D catalogs.Utilizing the Markov Chain Monte Carlo(MCMC)method for model fitting and Bayesian Information Criterion for model comparison,we find that compared to CDM,both FDM and SIDM haloes generally provide better fits to the observed rotation curves.Our findings reveal that the concentration–mass relation derived from the dark matter-only simulations is not followed by concentrations or masses obtained from the rotation curve data.Our analysis highlights a positive correlation between the core sizes of FDM and SIDM haloes and the effective radius of the galaxy,attributable to gravitational couplings between baryonic and dark matter components.Moreover,our exploration of dark matter fractions at characteristic radii indicates considerable diversity in dark matter distributions across dwarf galaxies.Notably,FDM and SIDM exhibit greater diversity than CDM in this respect. 展开更多
关键词 galaxies:dwarf galaxies:kinematics and dynamics galaxies:haloes (cosmology:)dark matter
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Multi-wavelength Study for Gamma-Ray Nova V1405 Cas
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作者 Zi-Wei Ou Pak-Hin Thomas Tam +2 位作者 Hui-Hui Wang Song-Peng Pei Wen-Jun Huang 《Research in Astronomy and Astrophysics》 2025年第2期224-231,共8页
Novae are found to have GeV to TeVγ-ray emission,which reveals the shock acceleration from the white dwarfs.Recently,V1405 Cas was reported to radiate suspiciousγ-ray by Fermi-LAT with low signi?cance(4.1σ)after th... Novae are found to have GeV to TeVγ-ray emission,which reveals the shock acceleration from the white dwarfs.Recently,V1405 Cas was reported to radiate suspiciousγ-ray by Fermi-LAT with low signi?cance(4.1σ)after the optical maximum.Radio observations reveal that it is one of the?ve brightest novae surrounded by low-density ionized gas columns.Here we report a continuous search for GeVγ-ray from Fermi-LAT.Noγ-ray was found.For V1405 Cas the?ux level is lower than other well-studied Fermi novae,and theγ-ray maximum appears at t0+145 days.γ-ray of V1405 Cas is used to search potentialγ-ray periodicity.Noγ-ray periodicity was found during the time of observation.By comparing multi-wavelength data,theγ-ray upper limit to optical?ux ratio with a value at around 10^(-4)is obtained to constrain the shock acceleration.Long-term analysis from Swift-XRT gets X-ray spectral in the post-shock phase,which indicates that V1405 Cas became a super-soft source.The best-?t black body temperature at the super soft state is 0.11-0.19 keV. 展开更多
关键词 gamma-rays:general (stars:)novae cataclysmic variables (stars:)white dwarfs
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Producing Type Ia Supernovae from Hybrid CONe White Dwarfs with Mainsequence Binary Companions at Low Metallicity of Z=0.0001
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作者 Boyang Guo Xiangcun Meng +2 位作者 Zhijia Tian Jingxiao Luo Zhengwei Liu 《Research in Astronomy and Astrophysics》 2025年第1期344-356,共13页
The nature of progenitors of Type Ia supernovae(SNe Ia)and their explosion mechanism remains unclear.It has been suggested that SNe Ia may have resulted from thermonuclear explosions of hybrid carbon-oxygen-neon white... The nature of progenitors of Type Ia supernovae(SNe Ia)and their explosion mechanism remains unclear.It has been suggested that SNe Ia may have resulted from thermonuclear explosions of hybrid carbon-oxygen-neon white dwarfs(CONe WDs)when they grow in mass to approach the Chandrasekhar mass limit by accreting matter from a binary main-sequence(MS)companion.In this work,we combine the results of detailed binary evolution calculations with population synthesis models to investigate the rates and delay times of SNe Ia in the CONe WD+MS channel at a low metallicity environment of Z=0.0001.For a constant star formation rate of 5M_(⊙)yr^(−1),our calculations predict that the SN Ia rates in the CONe WD+MS channel at low metallicity of Z=0.0001 is about 0.11−3.89×10^(−4) yr^(−1).In addition,delay times in this channel cover a wide range of 0.05−2.5 Gyr.We further compare our results to those given by a previous study for the CONe WD+MS channel with a higher metallicity of Z=0.02 to explore the influence of metallicity on the results.We find that these two metallicity environments give a slight difference in rates and delay times of SNe Ia from the CONe WD+MS channel,although SNe Ia produced at a low metallicity environment of Z=0.0001 have relatively longer delay times. 展开更多
关键词 methods numerical-(stars:)binaries(including multiple) close-stars evolution-(stars:)supernovae general-(stars:)white dwarfs
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Mode Identification Revisit and Asteroseismology of the DAV Star TIC 231277791
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作者 Z.K.Yao Y.H.Chen M.Y.Tang 《Research in Astronomy and Astrophysics》 2025年第4期235-243,共9页
White dwarfs(WDs)are the final stage for most low and intermediate mass stars,which play an important role in understanding stellar evolution and galactic history.Here we performed an asteroseismological analysis on T... White dwarfs(WDs)are the final stage for most low and intermediate mass stars,which play an important role in understanding stellar evolution and galactic history.Here we performed an asteroseismological analysis on TIC 231277791 based on 10 independent modes reported by Romero et al.Two groups of modes were identified with frequency splitting:mode identification_1 with one l=1,m=0 mode,two l=2,m=0 modes,and three l=1 or 2,m=0 modes,and mode identification_2 with one l=1,m=0 mode,three l=2,m=0 modes,and one l=1 or 2,m=0 mode.The rotation period is derived to be 41.64±2.73 hr for TIC 231277791.We established a large sample(7,558,272)of DAV star models using the White Dwarf Evolution Code(WDEC;2018,v16),resulting of optimal models with model_1(mode identification_1):M_(*)=0.570±0.005 M⊙,Teff=11300±10 K,-log(MH/M_(*))=9.15±0.01,-log(M_(He)/M_(*))=4.94±0.01,andσrms=0.06 s,and model_2(mode identification_2):M_(*)=0.720±0.005 M⊙,Teff=1910±10 K,-log(M_(H)/M_(*))=6.11±0.01,-log(M_(He)/M_(*))=3.09±0.01,andσrms=0.04 s.The central oxygen abundances are 0.71(optimal model_1)and 0.72(optimal model_2),respectively,which are consistent with the results of stellar structure and evolution theory. 展开更多
关键词 ASTEROSEISMOLOGY (stars:)white dwarfs (stars:)pulsars:individual(TIC 231277791)
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A Helium Nova[HP99]159:Past,Present and Future
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作者 Xin-Jie Luo Dong-Dong Liu Bo Wang 《Research in Astronomy and Astrophysics》 2025年第9期148-153,共6页
Type Ia supernovae(SNe Ia)are among the most extreme phenomena in the Universe and play a crucial role in the studies of stellar evolution,galactic chemical evolution and cosmology.However,the progenitors of SNe Ia ar... Type Ia supernovae(SNe Ia)are among the most extreme phenomena in the Universe and play a crucial role in the studies of stellar evolution,galactic chemical evolution and cosmology.However,the progenitors of SNe Ia are still under debate.It has been suggested that SNe Ia could be produced by helium(He)novae in the singledegenerate channel.Recently,a He nova named[HP99]159 was proposed to be a progenitor candidate for SNe Ia,in which the white dwarf(WD)has a mass of 1.2_(0.4)^(+0.18)M_(⊙),the He star’s mass ranges from 0.8M_(⊙)to 2.0M_(⊙)and the orbital period was suggested to be 2.33 or 1.16 days.In the present work,we evolved a large number of primordial binaries to the formation of WD+He star systems and investigated their future evolution.We provided a representative evolutionary track of[HP99]159 and found that[HP99]159 may originate from a primordial binary with a 6.16M_(⊙)primary and a 4.32M_(⊙)secondary with an initial orbital period of 5110 days.We also found that[HP99]159 might evolve to an SN Ia explosion as suggested by previous studies,or it may also form an accretion-induced collapse event or a wide double WD.Further detailed observations are needed in future studies to provide more information about the precise nature of[HP99]159. 展开更多
关键词 (stars:)binaries(including multiple) close-stars individual([HP99]159)-stars evolution-(stars:)supernovae general-(stars:)white dwarfs
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A Double-degenerate Scenario with a Merger to Explosion Delay Time to Explain Type Ia Supernova SN 2020aeuh
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作者 Noam Soker 《Research in Astronomy and Astrophysics》 2025年第11期1-5,共5页
I suggest the double-degenerate(DD)scenario with a merger-to-explosion delay(MED)time(the DD-MED scenario)of about 1-2 yr to explain the rare properties of the recently analyzed typeⅠa supernova(SN Ia)SN2020aeuh.The ... I suggest the double-degenerate(DD)scenario with a merger-to-explosion delay(MED)time(the DD-MED scenario)of about 1-2 yr to explain the rare properties of the recently analyzed typeⅠa supernova(SN Ia)SN2020aeuh.The rare properties are the SNⅠa ejecta interacting with a carbon-oxygen(CO)-rich circumstellar material(CSM)at approximately 50 days post-explosion.In this DD-MED scenario,two massive CO white dwarfs(WDs),with masses of M_(1)■1.1M_(☉)and M_(2)■M_(☉),merge to leave a rapidly rotating lonely WD of about the Chandrasekhar mass.The merger process ejects M_(CSM)■0.7M_(☉)to form a nonspherical CO-rich CSM.At the explosion,there is a lonely WD and a detached hydrogen-and helium-deficient CSM.Studies proposed the other lonely WD scenario,the core-degenerate(CD)scenario,to explain several specific SNe Ia and SN Ia remnants.SN 2020aeuh is the first particular SN Ia that is attributed to the DD-MED scenario.Besides being slightly brighter than typical SNe Ia and the CSM interaction,SN 2020aeuh is a normal SN Ia.Therefore,this study strengthens the claim of earlier studies,which are based on other arguments,like the properties of SN Ia remnants,that the lonely WD scenarios,i.e.,the DD-MED and CD scenarios,might account for most,if not all,normal SNe Ia.These earlier studies also argue that all SN Ia scenarios,whether lonely WD or not,might contribute to peculiar SNe Ia. 展开更多
关键词 (stars:)white dwarfs (stars:)supernovae:general (stars:)supernovae:individual(SN 2020aeuh) (stars:)binaries(including multiple):close
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水稻矮化并花发育异常突变体dwarf and deformed flower 2(ddf2)的基因定位与候选基因分析 被引量:8
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作者 张玲 郭爽 +5 位作者 汪玲 张天泉 庄慧 龙珏臣 何光华 李云峰 《中国农业科学》 CAS CSCD 北大核心 2015年第10期1873-1881,共9页
【目的】对一个同时导致营养和生殖器官发育异常的水稻突变体进行表型鉴定、基因定位和候选基因分析,为下一步的基因克隆与功能分析奠定基础。【方法】在水稻籼型恢复系602组织培养后代中,发现一个矮化并花发育异常突变体dwarf and defo... 【目的】对一个同时导致营养和生殖器官发育异常的水稻突变体进行表型鉴定、基因定位和候选基因分析,为下一步的基因克隆与功能分析奠定基础。【方法】在水稻籼型恢复系602组织培养后代中,发现一个矮化并花发育异常突变体dwarf and deformed flower 2(ddf2)。抽穗期,以野生型为对照,对ddf2株高、主穗长、节间和功能叶的长宽等性状进行统计分析;同时利用冷冻切片等技术对茎、叶和花器官进行详细的形态和组织学分析。分别以西农1A和中花11为母本,以DDF2/ddf2杂合株系为父本构建2个F2群体进行遗传分析和基因定位,并对候选基因进行实时荧光定量PCR(real-time PCR)分析。【结果】相较于野生型,突变体各节间的长和茎粗均极显著降低,叶片极显著变短、变窄,同时花序也极显著变短。组织细胞学分析发现,突变体大叶脉数目和相邻2个大叶脉之间的小叶脉数都没有明显的变化,但相邻2个大叶脉之间的宽度明显减小,进一步比较2个小叶脉之间的叶肉细胞,发现在突变体中细胞数目和尺寸均显著降低;突变体茎秆维管束的数目与野生型相比没有明显的变化,但统计发现2个大维管束之间基本组织细胞的数量和细胞的大小都显著小于野生型,表明ddf2突变体茎、叶细胞分裂和膨胀都受到了抑制;此外,ddf2突变体的花器官特征发育受到了严重干扰:第一轮外稃顶部弯曲、内稃不同程度退化,第三轮雄蕊器官严重退化,部分甚至转化为雌蕊状器官,另外部分ddf2小穗的护颖过度发育,转变成稃片状,一些小穗还表现分生组织确定性的丢失,发育出2个以上的小花。遗传分析表明该突变性状受1对隐性基因控制。利用中花11/ddf2的1 024株F2分离群体,最终将DDF2精细定位在第11染色体短臂近着丝粒位置处,位于insertion/deletion(in/del)标记S-11和S-14之间,遗传距离分别为0.049和0.098c M,物理距离为90.295 kb,并与标记S-24共分离。分析定位区间的基因,发现共有MSU注释基因12个,其中一个编码Sec3_C蛋白的LOC_Os11g17600内部包含共分离标记S-24,进一步对该基因进行表达分析,发现该基因在突变体的叶、茎和穗中都表现出明显的下调,初步将LOC_Os11g17600作为DDF2候选基因。【结论】DDF2是一个同时控制水稻茎/叶和花器官发育的新基因。 展开更多
关键词 水稻 矮化 花器官 DWARF and DEFORMED FLOWER 2(ddf2) 基因定位
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Molecular Tagging of a New Resistance Gene to Maize Dwarf Mosaic Virus Using Microsatellite Markers 被引量:6
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作者 吴建宇 汤继华 +1 位作者 夏宗良 陈伟程 《Acta Botanica Sinica》 CSCD 2002年第2期177-180,共4页
With joint analysis based on the parents, F 1, F 2 and backcrosses, the authors found that the resistance of the maize inbred line Huangzaosi to the maize dwarf mosaic virus strain B was conditioned by a major gene ... With joint analysis based on the parents, F 1, F 2 and backcrosses, the authors found that the resistance of the maize inbred line Huangzaosi to the maize dwarf mosaic virus strain B was conditioned by a major gene and polygene, and identified a new major gene. Bulked segregate and microsatellite analysis of a F 2 progeny from the combination of Huangzaosi×Mo17 were used to identify the resistance gene, mdm1(t), on the long arm of chromosome 6. This new resistance gene is tightly linked to and located between the microsatellite markers loci, phi077 and bnlg391. The linkage distances between phi077-mdm1(t) and mdm1(t)-bnlg391 are 4.74 centiMorgan (cM) and 6.72 cM respectively. 展开更多
关键词 MAIZE maize dwarf mosaic virus resistance gene MICROSATELLITE molecular tagging
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两个新水稻 Dwarf18 基因强等位突变体的表型分析及分子鉴定 被引量:3
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作者 侯雷 袁守江 +4 位作者 尹亮 赵金凤 万国峰 张文会 李学勇 《作物学报》 CAS CSCD 北大核心 2012年第8期1416-1424,共9页
通过EMS诱变日本晴获得了s2-9和s1-146a两个矮秆突变体,其植株矮小,成熟期株高分别为日本晴的26.3%和19.2%;苗期叶片较宽,叶色深绿;穗型仍为散穗但穗长变短,粒型未发生改变。对水稻胚乳的α-淀粉酶诱导实验表明,这2个矮秆突变体与GA的... 通过EMS诱变日本晴获得了s2-9和s1-146a两个矮秆突变体,其植株矮小,成熟期株高分别为日本晴的26.3%和19.2%;苗期叶片较宽,叶色深绿;穗型仍为散穗但穗长变短,粒型未发生改变。对水稻胚乳的α-淀粉酶诱导实验表明,这2个矮秆突变体与GA的信号传导途径无关,外源活性GA3对水稻幼苗株高的促进实验显示它们应与赤霉素的生物合成有关。利用突变体与籼稻品种Dular分别杂交构建了F2群体,精细定位表明这2个突变体的表型与水稻Dwarf18(D18)基因紧密连锁。序列分析发现这2个矮秆突变体的D18基因均发生了突变,在s2-9突变体中D18基因的内含子3'拼接点发生单碱基突变,s1-146a中D18基因编码区的单碱基突变导致提前终止密码子的出现。RT-PCR结果显示,在s1-146a中D18基因表达明显增强,但在s2-9中未检测到D18基因的表达。 展开更多
关键词 水稻 矮秆突变体 Dwarf18 赤霉素 精细定位
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Molecular Tagging and Effect Analysis of a New Small Grain Dwarf Gene in Rice 被引量:4
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作者 李秀兰 吴成 +3 位作者 邓晓建 王平荣 李仁端 杨志荣 《Acta Botanica Sinica》 CSCD 2003年第7期757-761,共5页
Plant height is one of the important agronomic traits of rice. Over higher plant would easily result in plant lodging and output reducing. On the other hand, the dwarf varieties with proper plant height had higher lod... Plant height is one of the important agronomic traits of rice. Over higher plant would easily result in plant lodging and output reducing. On the other hand, the dwarf varieties with proper plant height had higher lodging resistance and a greater harvest index, allowing for the increased use of nitrogen fertilizer. Dwarf breeding had made a great breakthrough in the rice breeding. The breeding and extension of excellent dwarf varieties remarkably improved the yield potential of rice. Therefore, the plant height is still one of the focuses in rice genetic research. 展开更多
关键词 Oryza sativa dwarf gene gene mapping microsatellite marker gene effect analysis
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棉花油菜素类固醇合成酶基因GhDWF1的克隆和表达分析 被引量:2
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作者 罗明 周建平 +4 位作者 肖月华 李先碧 侯磊 肖忠意 裴炎 《中国农业科学》 CAS CSCD 北大核心 2007年第7期1337-1344,共8页
【目的】研究油菜素类固醇物质(BRs)在棉花纤维生长发育过程中的作用。【方法】通过棉花EST序列的筛选和整合,从陆地棉徐州142纤维中克隆了一个BRs合成酶基因GhDWF1。【结果】其cDNA序列全长1849bp,包含一个1692bp的开放阅读框,推导编码... 【目的】研究油菜素类固醇物质(BRs)在棉花纤维生长发育过程中的作用。【方法】通过棉花EST序列的筛选和整合,从陆地棉徐州142纤维中克隆了一个BRs合成酶基因GhDWF1。【结果】其cDNA序列全长1849bp,包含一个1692bp的开放阅读框,推导编码563个氨基酸,与水稻、玉米、豌豆、番茄和拟南芥的BRs合成酶DWARF1/DIMINUTO有较高的同源性,具有该类蛋白的典型结构。GhDWF1基因在开花当天的胚珠和纤维中表达最强,在根、幼茎和纤维发育的各个时期表达较高。与徐州142野生型相比,无绒无絮突变体胚珠中表达量较低。【结论】暗示GhDWF1基因的表达与棉花纤维的生长发育有密切关系。 展开更多
关键词 棉花 DWARF1基因 纤维 油菜素类固醇 植物固醇
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