In order to effectively improve meshing performance of spiral bevel and hypoid gears generated by the duplex helical method, the effects of straight lined and circular cutting edges profile on meshing and contact of s...In order to effectively improve meshing performance of spiral bevel and hypoid gears generated by the duplex helical method, the effects of straight lined and circular cutting edges profile on meshing and contact of spiral bevel and hypoid gears were investigated analytically. Firstly, a mathematical model of spiral bevel and hypoid gears with circular blade profile was established according to the cutting characteristics of the duplex helical method. Based on a hypoid gear drive, the tooth bearings and the functions of transmission errors of four design cases were analyzed respectively by the use of the tooth contact analysis(TCA), and the contact stresses of the four design cases were analyzed and compared using simulation software. Finally, the curvature radius of the circular profile blade was optimized. The results show that the contact stresses are availably reduced, and the areas of edge contact and severe contact stresses can be avoided by selecting appropriate circular blade profile. In addition, the convex and concave sides are separately modified by the use of different curvature radii of inside and outside blades, which can increase the flexibility of the duplex helical method.展开更多
In this work,synchronous cutting of concave and convex surfaces was achieved using the duplex helical method for the hypoid gear,and the problem of tooth surface error correction was studied.First,the mathematical mod...In this work,synchronous cutting of concave and convex surfaces was achieved using the duplex helical method for the hypoid gear,and the problem of tooth surface error correction was studied.First,the mathematical model of the hypoid gears machined by the duplex helical method was established.Second,the coordinates of discrete points on the tooth surface were obtained by measurement center,and the normal errors of the discrete points were calculated.Third,a tooth surface error correction model is established,and the tooth surface error was corrected using the Levenberg-Marquard algorithm with trust region strategy and least square method.Finally,grinding experiments were carried out on the machining parameters obtained by Levenberg-Marquard algorithm with trust region strategy,which had a better effect on tooth surface error correction than the least square method.After the tooth surface error is corrected,the maximum absolute error is reduced from 30.9μm before correction to 6.8μm,the root mean square of the concave error is reduced from 15.1 to 2.1μm,the root mean square of the convex error is reduced from 10.8 to 1.8μm,and the sum of squared errors of the concave and convex surfaces was reduced from 15471 to 358μm^(2).It is verified that the Levenberg-Marquard algorithm with trust region strategy has a good accuracy for the tooth surface error correction of hypoid gear machined by duplex helical method.展开更多
The paper aimed to establish a duplex PCR method for simultaneous detection of Pseudomonas savastanoi pv. Phaseolicola （Psp） and Curtobaeterium /accumfadens pv. Flaccumfaciens （Cff）. Based on the argK gene of Psp ...The paper aimed to establish a duplex PCR method for simultaneous detection of Pseudomonas savastanoi pv. Phaseolicola （Psp） and Curtobaeterium /accumfadens pv. Flaccumfaciens （Cff）. Based on the argK gene of Psp in GenBank, the primers PSPF1/PSPR2 were designed. The duplex PCR assay was dereloped using the combined primers PSPF1/PSPR2 and CflF1/CffR2, which were specific primers for Cff. The reaction conditions were optimized and specificity md sensitivity of the duplex PCR were tested. The expected DNA fragment was specifically amplified from the genomic DNA of Psp and Cff. Specificity was conirmed in the artificially inoculated soybean samples imparted. Thus, the duplex PCR developed in this study could be used for the simultaneous detection of Psp md Cff from imported soybean.展开更多
基金Project(2011CB706800-G)supported by the National Basic Research Program of ChinaProject(51375159)supported by the National Natural Science Foundation of China+1 种基金Project(20120162110004)supported by the Postdoctoral Science Foundation of ChinaProject(2015JJ5020)supported by the Science Foundation of Hunan Province,China
文摘In order to effectively improve meshing performance of spiral bevel and hypoid gears generated by the duplex helical method, the effects of straight lined and circular cutting edges profile on meshing and contact of spiral bevel and hypoid gears were investigated analytically. Firstly, a mathematical model of spiral bevel and hypoid gears with circular blade profile was established according to the cutting characteristics of the duplex helical method. Based on a hypoid gear drive, the tooth bearings and the functions of transmission errors of four design cases were analyzed respectively by the use of the tooth contact analysis(TCA), and the contact stresses of the four design cases were analyzed and compared using simulation software. Finally, the curvature radius of the circular profile blade was optimized. The results show that the contact stresses are availably reduced, and the areas of edge contact and severe contact stresses can be avoided by selecting appropriate circular blade profile. In addition, the convex and concave sides are separately modified by the use of different curvature radii of inside and outside blades, which can increase the flexibility of the duplex helical method.
基金Projects(52075552,51575533,51805555,11662004)supported by the National Natural Science Foundation of China。
文摘In this work,synchronous cutting of concave and convex surfaces was achieved using the duplex helical method for the hypoid gear,and the problem of tooth surface error correction was studied.First,the mathematical model of the hypoid gears machined by the duplex helical method was established.Second,the coordinates of discrete points on the tooth surface were obtained by measurement center,and the normal errors of the discrete points were calculated.Third,a tooth surface error correction model is established,and the tooth surface error was corrected using the Levenberg-Marquard algorithm with trust region strategy and least square method.Finally,grinding experiments were carried out on the machining parameters obtained by Levenberg-Marquard algorithm with trust region strategy,which had a better effect on tooth surface error correction than the least square method.After the tooth surface error is corrected,the maximum absolute error is reduced from 30.9μm before correction to 6.8μm,the root mean square of the concave error is reduced from 15.1 to 2.1μm,the root mean square of the convex error is reduced from 10.8 to 1.8μm,and the sum of squared errors of the concave and convex surfaces was reduced from 15471 to 358μm^(2).It is verified that the Levenberg-Marquard algorithm with trust region strategy has a good accuracy for the tooth surface error correction of hypoid gear machined by duplex helical method.
基金Supported by Science and Technology Project of AQSIQ(2013IK277)National Rice Industry System Development Program
文摘The paper aimed to establish a duplex PCR method for simultaneous detection of Pseudomonas savastanoi pv. Phaseolicola （Psp） and Curtobaeterium /accumfadens pv. Flaccumfaciens （Cff）. Based on the argK gene of Psp in GenBank, the primers PSPF1/PSPR2 were designed. The duplex PCR assay was dereloped using the combined primers PSPF1/PSPR2 and CflF1/CffR2, which were specific primers for Cff. The reaction conditions were optimized and specificity md sensitivity of the duplex PCR were tested. The expected DNA fragment was specifically amplified from the genomic DNA of Psp and Cff. Specificity was conirmed in the artificially inoculated soybean samples imparted. Thus, the duplex PCR developed in this study could be used for the simultaneous detection of Psp md Cff from imported soybean.
